| 黄鳝出血病三联微胶囊口服疫苗制备及免疫效果研究 |
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| 引用本文： | 杨宗英,王玉兰,曾柳根,许亮清.黄鳝出血病三联微胶囊口服疫苗制备及免疫效果研究[J].南方农业学报,2016(6):1039-1044. |
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| 作者姓名： | 杨宗英 王玉兰 曾柳根 许亮清 |
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| 作者单位： | 南昌市农业科学院,南昌,330038 |
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| 基金项目： | 南昌市农业科技支撑计划项目(洪财企[2012]80号) |
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| 摘 要： | 【目的】制备出能有效防御黄鳝出血病的三联微胶囊口服疫苗,促进黄鳝养殖业健康发展。【方法】以嗜水气单胞菌、弗氏柠檬酸杆菌及温和气单胞菌为抗原,海藻酸钠为壁材,利用乳化法制备三联微胶囊口服疫苗,然后直接拌入饵料对健康黄鳝进行口服免疫,7 d后进行加强免疫,测定各项血清免疫指标。加强免疫结束15 d后,经腹腔注射3种活菌(1.0×109 CFU/m L)进行攻毒试验,检验三联微胶囊口服疫苗对黄鳝的免疫保护率。【结果】制备获得的三联微胶囊口服疫苗的平均粒径为44.5μm,平均含菌量为4.56×109 CFU/mg。按0.4和0.6 g/kg的剂量口服免疫黄鳝,其血清补体C3含量、超氧化物歧化酶(SOD)活性、酸性磷酸酶(ACP)活性均显著高于对照组(P0.05),血清平均抗体效价分别为7.5949和11.0651,对应的免疫保护率为60%和65%,且0.6 g/kg剂量组的各项指标均高于0.4 g/kg剂量组。【结论】制备获得的三联微胶囊口服疫苗能显著提高黄鳝血清SOD、ACP活性及刺激血清补体C3产生,有效抵御由嗜水气单胞菌、温和气单胞菌和弗氏柠檬酸杆菌引起的出血病,生产使用时须进行加强免疫,并适当加大免疫剂量。
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| 关 键 词： | 黄鳝 出血病 三联微胶囊口服疫苗 补体C3 超氧化物歧化酶 酸性磷酸酶 抗体效价 免疫保护率 |
Preparation of triple microencapsulated oral vaccine for Monopterus albus hemorrhagic septicemia disease and its immune effect |
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| Abstract: | Objective]The triple microencapsulated oral vaccine was prepared to defend against hemorrhagic sep-ticemia disease of Monopterus albus, in order to promote development of M. albus aquaculture industry. Method]Using Aeromonas hydrophila, Citrobacter freundii and Aeromonas sobria as antigens and sodium alginate as shell-material, the triple microencapsulated oral vaccine was prepared with emulsification method , and healthy M. albus were intragastrically immunized with the triple microencapsulated vaccine added into the basic diet, and booster immunization was conducted, then the immune indexes were determined. After 15 days of booster immunization, three alive strains(1.0×109 CFU/mL) were injected to enterocoelia of M. albus, so as to determine immune protective rate of triple microencapsulated oral vac-cine. Result]The average particle size of prepared microencapsulated vaccine was 44.5 μm, and the average bacterial content was 4.56×109 CFU/mg. After taking orally triple microencapsulated oral vaccine of 0.4 and 0.6 g/kg, the superox-ide dismutase(SOD) activity, acid phosphatase(ACP) activity, alexine C3 content in serum of M. albus were signifi-cantly higher than those in control group(P<0.05), and the corresponding average antibody titer in serum were 7.5949 and 11.0651, respectively, the corresponding immune protective rates were 60%and 65%, respectively. All the indexes of M. albus in 0.6 g/kg dosage group were higher than those in 0.4 g/kg dosage group. Conclusion]The triple microencapsu-lated vaccine can improve the activities of SOD and ACP, and stimulate production of alexine C3, it is effective to help M. albus to defend against hemorrhagic septicemia disease induced by A. hydrophila, C. freundii and A. sobria. Further-more, booster immunization should be conducted, and dosage should be increased properly in aquaculture. |
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| Keywords: | Monopterus albus hemorrhagic septicemia disease triple microencapsulated oral vaccine alexine C3 SOD activity ACP activity antibody titer immune protective rate |
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