| 辣木果荚腐病病原菌分离及鉴定 |
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| 引用本文： | 蔡志英,曾艳华,熊斌,刘一贤,杨焱,龙继明,殷振华,郭涵,李海泉.辣木果荚腐病病原菌分离及鉴定[J].南方农业学报,2016,47(9):1517-1521. |
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| 作者姓名： | 蔡志英 曾艳华 熊斌 刘一贤 杨焱 龙继明 殷振华 郭涵 李海泉 |
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| 作者单位： | 云南省热带作物科学研究所,云南 景洪,666100勐腊县勐醒农场 农林水综合服务中心,云南 勐腊,666304景洪市气象局,云南 景洪,666100 |
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| 基金项目： | 农业部“948”计划项目(2015-Z72)；云南省国际科技合作项目(2014ID010) |
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| 摘 要： | 【目的】明确辣木果荚腐病病原菌种类及其分类地位,为辣木果荚腐病防治提供理论依据。【方法】对云南省西双版纳州辣木种植基地感病、带黑色颗粒物病残体的辣木果荚进行症状观察、病原菌分离培养、致病性测定、显微形态特征观察、ITS和β-tubulin基因序列分析。【结果】辣木果荚腐病病原菌的分生孢子不分隔、透明,孢子顶端呈尖锥形弯曲,基部平截,具油滴,大小为15.7~22.1μm×2.5~4.3μm;厚垣孢子壁厚,呈黑褐色,簇生或成链状。该菌株ITS和β-tubulin基因的序列与Colletotrichum chlorophyti菌株IMI103806的同源性高达99%(ITS登录号:GU227894;TUB2登录号:GU228188)。多基因联合系统发育分析结果表明,供试菌株与兰生炭疽菌(C. chlorophyti)具有很近的遗传关系。【结论】兰生炭疽菌(C. chlorophyti)能侵染辣木引起果荚腐病,是为害辣木果荚的致病菌。
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| 关 键 词： | 辣木 果荚腐烂病 致病菌 鉴定 |
Isolation and identification of pathogens causing pod rot of Moringa oleifera |
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| CAI Zhi-ying,ZENG Yan-hua,XIONG Bin,LIU Yi-xian,YANG Yan,LONG Ji-ming,YIN Zhen-hua,GUO Han,LI Hai-quan.Isolation and identification of pathogens causing pod rot of Moringa oleifera[J].Journal of Southern Agriculture,2016,47(9):1517-1521. |
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| Authors: | CAI Zhi-ying ZENG Yan-hua XIONG Bin LIU Yi-xian YANG Yan LONG Ji-ming YIN Zhen-hua GUO Han LI Hai-quan |
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| Abstract: | Objective]In order to provide theory basis for controlling pod rot of Moringa oleifera, the pathogen was i-dentified, and its taxonomic status was studied. Method]The infected M. oleifera pods with black particles were collected from M. oleifera planting base in Xishuangbanna, Yunnan province. Based on disease symptom observation, morphological characteristics of pathogen, pathogenicity, rDNA-ITS and β-tubulin gene sequence analysis, the pathogen causing pod rot of M. oleifera was identified. Result]The conidia of pathogen were aseptate, hyaline, smooth, curved with tapered ends and a truncated base, and ranged from 15.7-22.1 μm×2.5-4.3 μm in size. Chlamydospores were dark brown, thick-walled, and fascicled or catenulate. The gene sequences of ITS and TUB2 gene showed 99% homology with Colletotrichum chlorophyti strain IMI103806 (ITS accession number: GU227894; TUB2 accession number: GU228188). Multi-gene phylogenetic analysis showed that the tested strain had the closest genetic relationship with C. chlorophyti. Conclusion]C. chlorophyti can cause pod rot of M. oleifera, which is pathogen damaging pod of M. oleifera. |
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| Keywords: | Moringa oleifera pod rot pathogen identification |
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