| 黑格尔七彩神仙鱼摩氏摩根菌分离鉴定及耐药性分析 |
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| 引用本文： | 王磊,孙培英,陈再忠,罗小溪,高建忠,李仲璞,韩灵莉.黑格尔七彩神仙鱼摩氏摩根菌分离鉴定及耐药性分析[J].南方农业学报,2016,47(10):1772-1777. |
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| 作者姓名： | 王磊 孙培英 陈再忠 罗小溪 高建忠 李仲璞 韩灵莉 |
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| 作者单位： | 上海海洋大学 水产与生命学院,上海,201306 |
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| 基金项目： | 上海市科技兴农重点攻关项目(沪农科种字[2015]第19号)；水产动物遗传育种中心上海市协同创新中心科研项目(ZF1206) |
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| 摘 要： | 【目的】对患病七彩神仙鱼进行摩氏摩根菌(Morganellamorganii)分离鉴定及药敏试验,为七彩神仙鱼养殖过程中摩氏摩根菌感染的临床诊断和科学用药提供科学依据。【方法】从患病野生黑格尔七彩神仙鱼体表分离病原菌,然后对其进行形态观察、生理生化指标测定及16S rDNA序列分析,并采用纸片扩散法(K-B)进行药敏试验。【结果】从患病野生黑格尔七彩神仙鱼分离获得一株优势菌株(DS2015),该菌为革兰氏阴性杆菌,其生理生化测定结果与摩氏摩根菌的相似度达100%。菌株DS2015的16S rDNA序列1208 bp(GenBank收录号KR866070),由基于16S rDNA序列构建的系统发育进化树可知,菌株DS2015与摩氏摩根菌聚为一簇,Bootstrap检验值为99;Biolog微生物自动鉴定结果也显示,菌株DS2015最具可能性为摩氏摩根菌。药敏试验结果表明,菌株DS2015对庆大霉素、新霉素、卡那霉素、哌拉西林、哌拉西林/他唑巴坦、头孢曲松、氯霉素、诺氟沙星、氧氟沙星和复方新诺明等抗生素高度敏感,对米诺环素中度敏感,对青霉素G、头孢氨苄、头孢拉定、四环素、多西环素、红霉素、克林霉素、万古霉素和呋喃唑酮等抗生素已产生耐药性。【结论】摩氏摩根菌可引起七彩神仙鱼身体发黑、皮肤大量分泌黏液及鳍条溃烂等症状,严重者甚至引起死亡,生产中可选用庆大霉素、新霉素、卡那霉素、哌拉西林、哌拉西林/他唑巴坦、头孢曲松、氯霉素、诺氟沙星、氧氟沙星和复方新诺明等抗生素进行防治。
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| 关 键 词： | 七彩神仙鱼 摩根氏菌 分离鉴定 药敏试验 |
Isolation,identification and drug resistance analysis of Morganella morganii from Symphysodon discus |
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| WANG Lei,SUN Pei-ying,CHEN Zai-zhong,LUO Xiao-xi,GAO Jian-zhong,LI Zhong-pu,HAN Ling-li.Isolation,identification and drug resistance analysis of Morganella morganii from Symphysodon discus[J].Journal of Southern Agriculture,2016,47(10):1772-1777. |
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| Authors: | WANG Lei SUN Pei-ying CHEN Zai-zhong LUO Xiao-xi GAO Jian-zhong LI Zhong-pu HAN Ling-li |
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| Abstract: | Objective]The present experiment was conducted to isolate and identify Morganella morganii from dis-eased Symphysodon discus and analyze its drug sensitivity, in order to provide scientific basis for clinical diagnosis and rational treatment of S. discus infected with M. morganii. Method]Pathogen was isolated from diseased S. discus, and then identified by observing its morphology, determining physiological and biochemical indexes, analysing 16S rDNA se-quence. Drug sensitivity was analyzed by Kirby-Bauer’s agar diffusion method. Result]Results showed that, one dom-inant bacterial strain DS2015 that was isolated from diseased S. discus was gram-negative bacterium and shared 100%similarity in physiological and biochemical indexes with M. morganii. Its 16S rDNA sequence was 1208 bp in length (Gen-Bank database No. KR866070). Phylogenetic tree based on 16S rDNA sequence indicated that strain DS2015 was clus-tered with M. morganii, and Bootstrap test value was 99. In addition, the identification result based on Biolog also showed that, strain DS2015 was most likely to be M. morganii. The drug sensitivity test indicated that, strain DS2015 was highly sensitive to gentamycin, neomycin, kanamycin, piperacillin, piperacillin/sulbactam, ceftriaxone, chloramphenicol, nor-floxacin, ofloxacin and sulfamethoxazole/trimethoprim, but was resistant to 9 drugs such as penicillin G, cefalexin, cefra-dine, tetracycline, doxycycline, erythromycin, clindamycin, vancomycin and furazolidone. Conclusion]M. morganii can cause S. discus infected symptoms such as body blackening, skin secreting massive mucus and fin festering, even lead to death in severe cases. Therefore, 10 kinds of antibiotics can be selected to treat S. discus infected with M. morganii, in-cluding gentamycin, neomycin, kanamycin, piperacillin, piperacillin/sulbactam, ceftriaxone, chloramphenicol, norfloxacin, ofloxacin and sulfamethoxazole/trimethoprim. |
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| Keywords: | Symphysodon discus Morganella morganii isoltation and identification drug sensitivity test |
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