| 重金属铜暴露对吉富罗非鱼组织残留及抗氧化酶活性的影响 |
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| 引用本文： | 单丹,钟欢,郭忠宝,周毅,肖俊,黄一帆,黄和明,罗永巨.重金属铜暴露对吉富罗非鱼组织残留及抗氧化酶活性的影响[J].南方农业学报,2016(10):1784-1789. |
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| 作者姓名： | 单丹 钟欢 郭忠宝 周毅 肖俊 黄一帆 黄和明 罗永巨 |
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| 作者单位： | 1. 上海海洋大学 水产与生命学院，上海 201306; 广西水产科学研究院，南宁 530021;2. 广西水产科学研究院,南宁,530021;3. 广西分析测试研究中心,南宁,530022 |
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| 基金项目： | 现代农业产业技术体系专项项目(CARS-49)；广西科学研究与技术开发计划项目(桂科重14121004-2-2)；广西科技基础条件平台建设计划项目(15-235-10B) |
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| 摘 要： | 【目的】探讨重金属铜在鱼体内的蓄积情况,并从抗氧化酶活性强弱变化分析铜暴露对鱼体组织造成的损害及其规律,为发展罗非鱼健康养殖提供参考依据。【方法】分别设0(CK)、1.0、2.0、4.0和6.0 mg/L不同铜暴露浓度的养殖水体,进行为期40 d的暴露试验后,取罗非鱼的鳃、肝脏、肾脏、肌肉和肠道组织测定其铜离子含量和抗氧化酶活性。【结果】各铜暴露处理组罗非鱼的肝体比均显著高于CK组(P0.05,下同)。在相同铜暴露浓度下,罗非鱼各组织富集铜离子的能力排序为肝脏肠道肾脏鳃肌肉。随着铜暴露浓度的升高,罗非鱼肝脏超氧化物歧化酶(SOD)活性先降低后升高,而肾脏SOD活性先升高后降低;在不同组织中,以肾脏过氧化氢酶(CAT)活性最强,且随铜暴露浓度升高呈先升高后降低的变化趋势,在2.0 mg/暴露浓度下其活性最强;丙二醛(MDA)含量在肝脏、肾脏中的变化规律与SOD活性一致,但转折的铜暴露浓度为4.0 mg/L。【结论】铜暴露能够使其富集在罗非鱼的不同组织中,其中以肝脏组织中的富集量最高,肌肉组织中的富集量较低,即对商品罗非鱼主要食用部分影响不明显。铜暴露对罗非鱼的毒害作用主要表现为肝脏和肾脏受损、体内代谢紊乱。
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| 关 键 词： | 吉富罗非鱼 铜 组织残留 抗氧化酶活性 |
Antioxidant enzyme activity and tissue residue of GIFT tilapia after copper exposure |
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| Abstract: | Objective]The present study was conducted to investigate copper accumulation in GIFT tilapia , analyzed the damages caused by copper in fish body based on enzyme activity variation and its regulation , in order to provide refer-ence for healthy culture of GIFT tilapia. Method]The experiment set different waters of 0 (CK),1.0, 2.0, 4.0 and 6.0 mg/L copper concentrations. After the fishes were cultured in corresponding waters for 40 days , copper ion contents and antioxidant enzyme activities of gill , liver , kidney , muscle and intestine were measured . Result]Liver and body weight ratio of GIFT tilapia in copper exposure treatments were significantly higher than that in control (P<0.05, the same below). In the same copper concentration exposure, accumulation ability of copper ions in different tissues were ranked as follows:liver>intestine>kidney>gill>muscle. As copper exposure concentration increased , SOD activity in liver reduced and then increased, whereas SOD activity in kidney increased first and then decreased. Among all tissues, CAT activity in kidney was the highest, and went through an up-down variation as copper exposure concentration increased. CAT activity in kidney reached maximum in 2.0 mg/L concentration. Variation regulations of MDA in liver and kidney were as the same as those of SOD activity, but the turning point was 4.0 mg/L concentration. Conclusion]Copper exposure leads to copper accumulation in different tissues in GIFT tilapia. Accumulation content in liver is the highest , whereas accumulation in muscle is low, which means it has insignificant influence on edible part of GIFT tilapia. Copper exposure causes damages in liver and kidney, and metabolic disorder. |
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| Keywords: | GIFT tilapia copper tissue residue antioxidant enzyme activity |
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