| 抑制黄瓜枯萎病的链霉菌S-101及其生防作用研究 |
| |
| 引用本文： | 圆圆,张虹,苏道拉呼,峥嵘,石玲玲,吴慧玲.抑制黄瓜枯萎病的链霉菌S-101及其生防作用研究[J].中国生物防治学报,2019,35(5):813-820. |
| |
| 作者姓名： | 圆圆 张虹 苏道拉呼 峥嵘 石玲玲 吴慧玲 |
| |
| 作者单位： | 1. 内蒙古师范大学生命科学与技术学院, 呼和浩特 010022;2. 北京市农林科学院植物保护环境保护研究所, 北京 100097 |
| |
| 基金项目： | 国家重点研发计划项目（2018YFD0201300）；北京市自然科学基金（6192008）；北京市农林科学院创新专项（KJCX20170410） |
| |
| 摘 要： | 为了获得抑制黄瓜枯萎病的高效生防菌，对青海湖附近植被根系土壤中的微生物进行分离筛选。测定拮抗菌的抑菌活性及其对黄瓜枯萎病的防控效果。本文通过平板对峙法分离筛选出一株生防链霉菌S-101，该菌株对供试的几种病原真菌都有较好的拮抗作用，其中对尖镰孢菌黄瓜专化型的抑制率为53.3%，对草莓炭疽菌的抑菌率为45.5%；并通过形态特征及16S rDNA序列分析对菌株S-101初步鉴定为Streptomyces luridus；盆栽试验结果表明，菌株S-101发酵液对黄瓜枯萎病的防治效果达57.11%；通过构建绿色荧光蛋白标记的基因工程菌S-101-GFP，检测了该菌株在黄瓜根部及根围土壤中的定殖能力，随着定殖天数的增加，数量由处理时的1×108 cfu/g逐渐减少到1×106 cfu/g，0～14 d下降较快，21～28 d下降较慢，28～35 d趋于稳定，孢子数维持在1×106 cfu/g。因此，菌株S-101是防治黄瓜枯萎病的潜在生防菌株，具有开发成为微生物农药的应用价值。
|
| 关 键 词： | 生防菌 黄瓜枯萎病 分离鉴定 生防效应 定殖 |
| 收稿时间： | 2019-01-15 |
Biocontrol Effect of Streptomyces sp. S-101 against Cucumber Wilt |
| |
| YUAN Yuan,ZHANG Hong,SU Daolahu,ZHENG Rong,SHI Lingling,WU Huiling.Biocontrol Effect of Streptomyces sp. S-101 against Cucumber Wilt[J].Chinese Journal of Biological Control,2019,35(5):813-820. |
| |
| Authors: | YUAN Yuan ZHANG Hong SU Daolahu ZHENG Rong SHI Lingling WU Huiling |
| |
| Affiliation: | 1. College of Life Sciences and Technology, Inner Mongolia Normal University, Hohhot 010022, China;2. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China |
| |
| Abstract: | In order to obtain efficient biocontrol bacteria against cucumber wilt, the microorganisms in the vegetation root soil near Qinghai Lake were isolated and screened. The activity of antagonistic bacteria and its control effect on cucumber wilt disease were determined. Streptomyces sp. strain S-101 was isolated and screened by plate confrontation method and its antagonism against different plant pathogens was investigated. The results showed that the strain had good effect on several pathogenic fungi, among which the inhibition rates on Fusarium oxysporum f. sp. cucumebrium and Colletotrichum gloeosporioides were 53.3% and 45.5%, respectively. Based on morphological observation and 16S rDNA sequence analysis, the strain S-101 was preliminarily identified as Streptomyces luridus. Pot experiment showed that the fermentation broth displayed significant control effect on cucumber wilt with the efficacy of 57.11%.The strain S-101-GFP was able to colonize at the roots of cucumber. With the increase of colonization days, the number of labeled bacteria decreased from 1×108 cfu/g to 1×106 cfu/g. The number of colonies dropped rapidly in 0-14 days, fell slowly in 21-28 days and maintained a stable amount in 28-35 days at 1×106 cfu/g. Therefore, strain S-101 is a potential biocontrol strain against cucumber wilt, and has the application value of being developed into microbial fungicide. |
| |
| Keywords: | biocontrol bacteria cucumber wilt isolation and identification biocontrol effect colonization |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物防治学报》浏览原始摘要信息 |
|
点击此处可从《中国生物防治学报》下载全文 |
|
