Enriching Artemia nauplii with a high DHA‐containing lipid emulsion: search for an optimal protocol |
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| Authors: | Elena Viciano Óscar Monroig Ana Salvador Jaume Amat Susana Fiszman Juan Carlos Navarro |
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| Affiliation: | 1. Departamento de Cultivo, Biología y Patología de Especies Marinas, Instituto de Acuicultura de Torre de la sal (IATS‐CSIC), Ribera de Cabanes, Castellón, Spain;2. Departamento de Conservación y Calidad de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos (IATA‐CSIC), Paterna, Valencia, Spain;3. Bioemprèn, Barcelona, Barcelona |
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| Abstract: | This study aimed to investigate practical strategies to optimize the use of a high‐docosahexaenoic acid (DHA) lipid emulsion (M70), a product with great potential in live prey enrichments for marine larviculture. Considering its particularly high content in DHA (22:6n‐3), the adequate utilization of the emulsion for Artemia enrichments was evaluated in a series of six experiments. More specifically, the bioencapsulation efficiency of M70 into Artemia nauplii was tested under different experimental conditions of oxygen source, aeration flow, incubation temperature, concentration and dosage, as well as nauplial densities. Our results showed that an optimal utilization of M70 is achieved with incubation temperatures of 28°C, moderate aeration flows and nauplial densities of 300 ind per mL. In addition, the emulsion can be dispensed in the enrichment medium in one single dose of 0.8 g L?1, with no apparent detrimental effects on its oxidative stability and Artemia nauplii survival during enrichment. |
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| Keywords: |
Artemia
docosahexaenoic acid essential fatty acid lipid emulsion live prey enrichment |
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