| 长臂虾科线粒体基因组结构与系统进化分析 |
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| 引用本文： | 朱陇强,朱志煌,林琪,朱雷宇,王定全,王健鑫.长臂虾科线粒体基因组结构与系统进化分析[J].中国水产科学,2021,28(7):852-862. |
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| 作者姓名： | 朱陇强 朱志煌 林琪 朱雷宇 王定全 王健鑫 |
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| 作者单位： | 浙江海洋大学海洋微生物分子生态与应用实验室, 浙江 舟山 316000 ；福建省水产研究所, 福建 厦门 361013 ；福建省海洋生物增养殖与高值化利用重点实验室, 福建 厦门 361013 |
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| 基金项目： | 国家重点研发计划项目(2019YFD0901305)； 福建省海洋与渔业结构调整专项(2020HYJG01, 2020HYJG08)； 舟山市科技局项目(2019C21011). |
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| 摘 要： | 综合分析了长臂虾科(Palaemonidae)14 个物种线粒体基因组的全序列, 发现长臂虾科线粒体基因组的长度为 15396~15967 bp, A+T 含量为 58.97%~69.09%; 长臂虾科内不同属线粒体基因组的基因排列各不相同, 与十足目 (Decapoda)线粒体基因组的原始排列相比, 除沼虾属(Macrobrachium)的基因排列顺序未发生改变外, 长臂虾属 (Palaemon)、叶颚虾属(Hymenocera)和贝隐虾属(Anchistus)的基因排列顺序均发生了不同程度的变化, 此外贝隐虾属(Anchistus)还出现基因缺失现象; 长臂虾属和沼虾属线粒体 13 个蛋白编码基因(protein-coding genes, PCGs)的 Ka/Ks<<1, 显示出很强的纯化选择; 长臂虾科 13 PCGs 在密码子的使用中, 被编码的氨基酸均体现相似的偏好性; 在线粒体基因组的差异位点分析中, 发现 nd5、nd4 和 nd2 基因为理想的分子标记。基于 13 PCGs 系统发育结果表明, 长臂虾属与沼虾属互为姊妹关系, 叶颚虾属和贝隐虾属单独聚为一大支。分子钟的估算结果推测长臂虾科物种可能起源于二叠纪, 随后进一步分化为具有现代表征的长臂虾种类。本研究为快速鉴定长臂虾科生物提供了可靠的分子标记, 并为分析长臂虾科物种遗传多样性提供理论基础。
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| 关 键 词： | 长臂虾科 线粒体基因组 结构特征 系统发育 |
Characteristics and phylogenetic analysis of the mitochondrial genome in Palaemonidae |
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| Zhu Longqiang,Zhu Zhihuang,Lin Qi,Zhu Leiyu,Wang Dingquan,Wang Jianxin.Characteristics and phylogenetic analysis of the mitochondrial genome in Palaemonidae[J].Journal of Fishery Sciences of China,2021,28(7):852-862. |
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| Authors: | Zhu Longqiang Zhu Zhihuang Lin Qi Zhu Leiyu Wang Dingquan Wang Jianxin |
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| Abstract: | The basic characteristics of Palaeminidae mitogenomes were revealed by the comprehensive mitogenome analysis of 14 Palaeminidae species. The results showed that the lengths of the 14 mitogenomes varied from 15396 bp to 15967 bp. The content of A + T ranged from 58.97% to 69.09%. Compared with the gene order of a Decapoda ancestor, the gene arrangement order of the Macrobrachium had not changed, but the gene arrangement order of Palaemon, Anchistus, and Hymenocera changed to varying degrees. Furthermore, gene deletion was detected in the mitogenome of Anchistus. The Ka/Ks ratios of 13 protein coding genes (PCGs) in Palaemon and Macrobrachium was much lower than one, indicating strong purification. In addition, the codon usage of the 13 PCGs in Palaeminidae showed that the encoded amino acids had similar preferences. Among the two rRNA genes and 13 PCGs identified the mitogenome, the analysis of the different sites between Palaemon and Macrobrachium showed that the genes nd5, nd4, and nd2 were ideal molecular markers. The phylogenetic trees based on the 13 PCGs showed that Palaemon and Macrobrachium were sisters, and Anchistus and Hymenocera were gathered together into a large branch. Finally, according to the estimation results of the molecular clock, it is speculated that Palaeminidae species may have originated in the Permian, before further differentiating into a species with modern characteristics. This study provides a reliable molecular marker for the rapid identification of Palaeminidae species and a theoretical basis for analyzing the genetic diversity of Palaeminidae species. |
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