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虎纹蛙白内障病病原的分离鉴定及其免疫防治
引用本文:周永灿,朱传华,等.虎纹蛙白内障病病原的分离鉴定及其免疫防治[J].上海海洋大学学报,2001(1):16-21.
作者姓名:周永灿  朱传华
作者单位:[1]海南大学水产系,海南海口570228 [2]中国水产科学院南海水产研究所,广东广州510300
摘    要:本文报道了海南养殖虎纹蛙白内障病病原及其免疫学防治方法。对该典型患病个体进行病原分离并鉴定表明,被分离出的病原为脑膜炎败血黄杆菌。在所实验的20种抗菌药物中,该菌只对万古霉素敏感,对头孢哌酮中度敏感,而对其他18种药物均不敏感。通过筛选该病原强毒株制备甲醛灭活疫苗,对健康虎纹蛙进行免疫接种后,可明显提高其对该病的抵抗能力,表明在生产上用免疫学方法防治该病是可行的。

关 键 词:虎纹蛙  脑膜炎败血黄杆菌  白内障病  分离  鉴定  免疫学防治
修稿时间:2000/10/30 0:00:00

Isolation and identification of pathogen of the cataract disease and its immunological control in Rana tigrina rugulosa
ZHOU Yong can,ZHU Chuan hu,CHEN Guo hu,SU Wen qiang,TANG Ze feng.Isolation and identification of pathogen of the cataract disease and its immunological control in Rana tigrina rugulosa[J].Journal of Shanghai Ocean University,2001(1):16-21.
Authors:ZHOU Yong can  ZHU Chuan hu  CHEN Guo hu  SU Wen qiang  TANG Ze feng
Abstract:This paper reports the pathogen and the immunological protective method of the cataract disease in cultured Rana tigrina rugulosa in Hainan. The pathogen isolated from the ill frog with typical characteristics was identified as Flavobacterium meningitidis. In all the 20 kinds of antimicrobial agents tested in this experiment, only vancomycin is susceptible and cefoperazone is intermediate susceptible to the pathogen, as to the other 18 kinds of antimicrobics are unsusceptible. After inoculation the health frogs with the vaccine prepared from the formalin killed Flavobacterium meningitidis, their sera agglutination titer and resistance to cataract disease improved obviously. It shows that the cataract disease in cultured Rana tigrina rugulosa can be successfully prevented by the vaccine.
Keywords:Rana tigrina rugulosa  Flavobacterium meningitidis  cataract disease  immunology  pathogen
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