Vaccination strategies with oral booster for surubim hybrid (Pseudoplatystoma corruscans × P. reticulatum) against haemorrhagic septicaemia |
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| Authors: | Gabriella do Vale Pereira Bruno Corrêa da Silva Felipe do Nascimento Vieira Walter Quadros Seiffert Thiago Tetsuo Ushizima José Luiz Pedreira Mouriño Maurício Laterça Martins |
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| Affiliation: | 1. Laboratory of Marine Shrimp – LCM, Aquaculture Department, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil;2. AQUOS – Aquatic Organisms Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil;3. Mar & Terra Ind. Com de pescados S/A, Research and Development Division, Mato Grosso do Sul, Brazil |
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| Abstract: | This study evaluated the efficiency of differently prepared vaccines against Aeromonas hydrophila in the hybrid surubim (Pseudoplatystoma corruscans × P. reticulatum). Survival and haemato‐immunological parameters were compared between the treatments: non‐vaccinated fish (C); bacterin‐vaccinated fish (B); bacterin plus oral booster vaccinated fish (B+O); bacterin and toxoid‐vaccinated fish (B+T) and bacterin, toxoid and oral booster‐vaccinated fish (B+T+O). Fourteen‐days vaccinated fish from B+O and B+T+O were fed with an oral booster for 4 days. After 1 week, the fish were intraperitoneally challenged with 2 × 108 CFU mL?1 of A. hydrophila. Fish from the treatment B+T+O showed the lowest cumulative mortality (11.36%) 96 h after challenge, compared with other treatments (22.72–44.04%), and a relative survival of 74%. Serum immunoglobulin in B+T+O fish was higher than in other treatments. All vaccinated fish showed an increased agglutination titre when compared with non‐vaccinated fish, both before and after challenge. Fish fed with oral booster showed an increase in phagocytic percentage before and after challenge. It can be inferred that the oral booster vaccination was efficient in reducing mortality in hybrid surubim by enhancing the response against haemorrhagic septicaemia due to A. hydrophila infection. |
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| Keywords: |
Pseudoplatystoma
Aeromonas hydrophila
immunization and oral booster |
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