| 北京地区番茄青枯病健康和发病植株根际细菌群落比较分析 |
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| 引用本文： | 马荣,马毅楠,王星,谷医林,魏海雷,张晓霞.北京地区番茄青枯病健康和发病植株根际细菌群落比较分析[J].中国生物防治学报,2022,38(4):920-928. |
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| 作者姓名： | 马荣 马毅楠 王星 谷医林 魏海雷 张晓霞 |
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| 作者单位： | 中国农业科学院农业资源与农业区划研究所/农业农村部农业微生物资源收集与保藏重点实验室, 北京 100081 |
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| 基金项目： | 国家重点研发计划(2019YFD1002000);现代农业产业体系北京市特色作物创新团队土壤生态评价与产品开发岗位;北京市乡村振兴科技项目农业微生物制剂创制与落地平谷产业化开发 |
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| 摘 要： | 本研究利用高通量测序技术研究了北京地区番茄青枯病健康与发病植株根际细菌组成差异,构建相关性网络,并从健康植株根际分离和筛选青枯菌拮抗菌群。结果显示,pH和速效磷是影响各采样地区土壤细菌群落组成的主要因子;发病植株根际土壤群落结构比健康植株更复杂,群落间相互作用数量增多;网络分析表明有15个属与Ralstonia直接相关;通过拮抗实验获得14株对青枯菌具有显著拮抗能力的根际细菌,其中包括3个不常见拮抗细菌类群,为无色杆菌属Achromobacter、剑菌属Ensifer和赖氨酸芽胞杆菌属Lysinibacillus。该研究结果为北京地区番茄青枯病预警和防控提供了理论基础和资源储备。
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| 关 键 词： | 高通量测序 番茄青枯病 根际群落 相关性网络 |
| 收稿时间： | 2022-06-26 |
Comparative Analysis of Healthy and Diseased Rhizosphere Bacterial Communities of Tomato Bacterial Wilt in Beijing Region |
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| MA Rong,MA Yinan,WANG Xing,GU Yilin,WEI Hailei,ZHANG Xiaoxia.Comparative Analysis of Healthy and Diseased Rhizosphere Bacterial Communities of Tomato Bacterial Wilt in Beijing Region[J].Chinese Journal of Biological Control,2022,38(4):920-928. |
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| Authors: | MA Rong MA Yinan WANG Xing GU Yilin WEI Hailei ZHANG Xiaoxia |
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| Affiliation: | Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081 |
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| Abstract: | In this study,the difference of rhizosphere bacteria composition between healthy and bacterial wilt tomato plants was investigated by high-throughput sequencing,and the correlation network was constructed.Antagonistic bacteria against Ralstonia solanacearum were isolated from the rhizosphere of healthy plants.It was found that soil pH and available phosphorus in different regions were the main factors to shape the rhizosphere bacterial community.The rhizosphere community structure of the diseased plants was more complex than that of healthy plants,which demonstrated by the increase of interactive nodes.Fifteen bacterial genera directly related to Ralstonia was defined.Fourteen strains of rhizosphere bacteria were obtained by antagonistic experiments,including three uncommon antagonistic groups,Achromobacter,Ensifer and Lysinibacillus.The results provided information for the early warning and control of tomato wilt in Beijing region. |
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| Keywords: | high-throughput sequencing tomato bacterial wilt rhizosphere community correlation network |
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