共查询到18条相似文献，搜索用时 406 毫秒
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《（《农业科学与技术》）编辑部》2008,(2)
[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [Method] The genomic DNAs were extracted from tender leaves of 24 peanut cultivars and from the liver,lung and kidney of white mouse through the specifically modified CTAB method. The DNAs were run on agarose gel,next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [Result] The clear and orderly bands were observed in gel detection,and the values of OD260/OD280 for DNAs extracted via modified CTAB method were between 1.77-1.83. The DNAs performed well in PCR amplification. [Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification. 相似文献
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A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method 总被引：1，自引：0，他引：1
YAN Miao-miao WEI Guang-cheng PAN Xiao-hong MA Huai-lei LI Wei-zhen Yantai Campus of Binzhou Medical College Yantai 《（《农业科学与技术》）编辑部》2008,(2)
[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [Method] The genomic DNAs were extracted from tender leaves of 24 peanut cultivars and from the liver,lung and kidney of white mouse through the specifically modified CTAB method. The DNAs were run on agarose gel,next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [Result] The clear and orderly bands were observed in gel detection,and the values of OD260/OD280 for DNAs extracted via modified CTAB method were between 1.77-1.83. The DNAs performed well in PCR amplification. [Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification. 相似文献
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大白菜基因组DNA的提取及AFLP反应体系的建立(英文) 总被引：1，自引：0，他引：1
[Objective] The obtained clear AFLP fingerprint of Chinese cabbage provided basis for studies on the molecular markers of Chinese cabbage cultivars and the phylogenetic relationship among Chinese cabbage cultivars. [Method] With the test materials of leaves of Chinese cabbages, the high-quality total DNA from leaves of Chinese cabbages was extracted by the modified CTAB method. DNA restriction-ligase reaction, pre-amplification and selective amplification were optimized, and the AFLP silver-staining reaction system for Chinese cabbage was established. [Result] The quality of DNA template influenced restriction enzyme digestion and the subsequent ligase amplification reaction, while the modified CTAB extraction method could be used in AFLP analysis of Chinese cabbage to obtain a clear AFLP fingerprint. The optimum conditions for restriction enzyme digestion of genomic DNA from Chinese cabbage were as follows: 150 g DNA template, 12.5 μl reaction volume, 1.25 U Eco R Ⅰ, 1.25 U Mse Ⅰ and 5×Reaction Buffer with 4 h at 37 ℃. The ligation reaction with 2.5 h at 20 ℃ was the optimum condition. Six pairs of primers including E-AAC/M-CAG, E-AAG/M-CAC, E-ACA/M-CTG, E-ACT/M-CAC, E-ACT/M-CTT and E-ACT/M-CTC all had its own stable and clear patterns. [Conclusion] With abundant bands and high polymorphism, AFLP selective amplification is an efficient molecular marker for genomic polymorphism of Chinese cabbage. 相似文献
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《（《农业科学与技术》）编辑部》2008,(2)
[Objective] The objective of this study is to explore a rapid and efficient method of extracting genomic DNA from Artemisia abrotanum. [Method] Three methods of Cutting [Method],Liquid Nitrogen [Method] and Quartz Sand [Method] based on SDS method were employed to extract Artemisia abrotanum genomic DNA from tender leaf at seedling stage,tender spike and old leaf at heading stage. The obtained DNAs were detected by absorbance detection,agarose gel and PCR amplification. [Result] Cutting [Method] performed better than the other two methods compared in purity,extracting cycle and cost,accordingly more suitable for PCR amplification. The results also show that young spike is the best material for extracting genomic DNA from Artemisia Annua. 相似文献
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An Improved Method of Extracting Artemisia abrotanum Genomic DNA 总被引：8，自引：0，他引：8
SHI Kai-ming ZHOU Yi-feng . College of Biology Science Technology Hubei Institute for Nationalities Enshi . Key Laboratory of Biological Resource Protection Utilization in Hubei Province Enshi . Key Laboratory of Biotechnology in Traditional Chinese Medicine of Hubei Province Hubei University Wuhan 《（《农业科学与技术》）编辑部》2008,(2)
[Objective] The objective of this study is to explore a rapid and efficient method of extracting genomic DNA from Artemisia abrotanum. [Method] Three methods of Cutting [Method],Liquid Nitrogen [Method] and Quartz Sand [Method] based on SDS method were employed to extract Artemisia abrotanum genomic DNA from tender leaf at seedling stage,tender spike and old leaf at heading stage. The obtained DNAs were detected by absorbance detection,agarose gel and PCR amplification. [Result] Cutting [Method] performed better than the other two methods compared in purity,extracting cycle and cost,accordingly more suitable for PCR amplification. The results also show that young spike is the best material for extracting genomic DNA from Artemisia Annua. 相似文献
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ZHANG Wen-hui GUO Hua WANG Wei-li LIU Ming QIAN Ai-dong .Jilin Agricultural University Changchun .Jilin Entry-Exit Inspection Quarantine Bureau of the People's Republic of China Changchun .National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute of CAAS Harbin 《（《农业科学与技术》）编辑部》2008,(1)
[Objective] The research aimed to design primers that are suitable for detecting H5 and H7 subtypes of avian influenza virus(AIV);[Method] DNAStar was used to analyze the homology of the sequences of H5 and H7 subtypes of AIV accessed in GenBank,and design primers(by Primer Premier 5.0)on high homologous region of these sequences,and then amplified by RT-PCR.[Result] The multiplex RT-PCR amplification,agarose gel electrophoresis and sequencing results showed that the self-designed primers are successful for detecting AIV.[Conclusion] It is feasible to rapidly diagnose AIV through this method. 相似文献
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The ISSR method was developed to analyze the genetic diversity of 20 wild Wusuli raccoon dog from Nenjiang district. The results showed that there were significant genetic diversity and high polymorphism among individuals of the wild Wusuli raccoon dog. A total of 41 DNA bands were amplified by 9 primers, and 37 of them were polymorphism, the proportion of polymorphism was 90.24%, 2-8 polymorphism bands could be amplified by each primer, and 4.56 on average, the length of the product was 200-2 000 bp. 相似文献
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CHEN Li-hong YAN Wei XU Yan 《中国农业科学(英文版)》2007,6(8):956-963
To identify Cenococcum geophilum Fr., estimate their genetic diversity and study the effects on their genetic variation, 27 Chinese C. geophilum isolates from 6 host plant species and 5 French C. geophilum isolates were analyzed using morphological and molecular methods. The universal primers ITS1/ITS4 were used in PCR-RFLP to amplify the rDNA internal transcribed spacer （ITS） of tested C. geophilum isolates. The amplified products were digested with EcoR Ⅰ, Hinf Ⅰ, and Mbo Ⅰ, and the digested fragments of PCR products showed that there were obvious differences. A random primer （5′-CGCACCGCAC-3′） was employed in RAPD to amplify the genomic DNA of C. geophilum, and 19 detectable and reliable DNA bands of 300-2000 bp size were observed. According to the number, position, and strength of the DNA bands in agarose gel, the genetic distance and the genetic similarity among C. geophilum isolates were calculated using the PopGen Ver. 1.31 dendrogram analysis software. A phylogenetic tree was constructed based on the genetic distance by the Neighbor-Joining/UPGMA in PHYLIP. The results suggest the high level of genetic diversity among C. geophilum isolates from the same or different hosts. The effects of geographical factors or host plant species on C. geophilum genetic variation are not obvious. 相似文献
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[目的]探索基于AFLP的拮抗链霉菌DNA模板制备方法及其扩增体系,为AFLP技术在链霉菌乃至放线菌资源分析中的应用提供依据。[方法]以改进的CTAB法提取DNA,利用Pst I／Mse I型AFLP试剂盒及其反应体系进行扩增,采用5％变性聚丙烯酰胺凝胶电泳分析扩增结果。[结果]提取了10个拮抗链霉菌菌株的基因组DNA,0.8％琼脂糖凝胶电泳检测显示其主带清晰,片段大小为37.64-40.86Kb,无降解现象,亦无RNA残留;其OD260/OD280为1.625-1.833;Pst I／Mse I双酶切产物琼脂糖电泳呈弥散荧光长带,说明酶解充分;筛选出的3对引物对DNA模板的扩增谱带清晰,多态性丰富。[结论]该研究建立的DNA模板制备方法及其扩增反应体系可用于链霉菌的AFLP分析。 相似文献
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A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method 总被引：6，自引：0，他引：6
[Objective] The study aimed to introduea a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [Method] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the spe-cifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [Result] The clear and orderly bands were observed in gel detection, and the val-ues of OD260/OD280 for DNAs extracted via modified CTAB method were between 1.77 - I. 83. The DNAs performed well in PCR amplification. [Conclu-sion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification. 相似文献
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辣椒细胞质雄性不育恢复基因的AFLP标记 总被引：2，自引：0，他引：2
采用AFLP技术对辣椒细胞质雄性不育系21A及其相应的恢复系湘紫基因组DNA进行多态性比较,筛选了64对AFLP选择性引物PstⅠ-NNN+MseⅠ-NNN组合,有58对引物组合在两系间扩增出5156条带,多态性位点为4个,主要表现为条带数目、条带位置和条带强弱的差异。其中在恢复系材料中仅有3个多态性片断,找到了辣椒细胞质雄性不育恢复系湘紫基因组DNA特异的AFLP片段ACA/CGC300、AGT/GGT375和AGC/CGC380,并对这些特异片段的来源及其在细胞质雄性不育恢复系中的作用进行了讨论。 相似文献
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通过采用不同的酶切组合,即EcoRⅠ/MseⅠ组合240对引物、PstⅠ/MseⅠ组合96对引物、PstⅠ/TaqⅠ组合96对引物,分别对花生高油酸品种Sunoleic95R和低油酸品种汕油162进行AFLP分析.分析了两亲本之间的差异,回收了72条AFLP多态性条带,在所选定高和低O/L比值亲本材料之间建立了AFLP... 相似文献
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[目的]探索并建立悬铃木方翅网蝽总DNA提取方法。[方法]采用改良SDS法结合Silica吸附柱提取悬铃木方翅网蝽总DNA,通过分光光度法、琼脂糖凝胶电泳对获得的DNA进行浓度、纯度及质量检测。利用PCR技术,分别扩增悬铃木方翅网蝽线粒体基因细胞色素氧化酶亚基Ⅰ（COⅠ）和基因组28S基因,克隆测序。[结果]悬铃木方翅网蝽总DNA浓度为52.8μg/ml,A260/A280为2.06,琼脂糖凝胶电泳条带清晰。PCR扩增产物条带清晰特异,经克隆并测序后表明为悬铃木方翅网蝽COⅠ和28S基因。[结论]该方法可得到较纯净完整和扩增效果良好的DNA,能满足进一步分子生物学研究的需要。 相似文献