共查询到20条相似文献，搜索用时 62 毫秒
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George JW O'Neill SL 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2001,30(1):16-18
Abstract: Most hand-held medical refractometers have internal scales that limit protein measurement to results ≥ 2.5 g/dL. Tables for conversion of refraction (r) to protein concentration for values as low as 0.1 g/dL were published in the 1960s, but their accuracy for use on body fluids has not been established. The purpose of this study was to assess the reliability of body cavity fluid protein determination by refractometry. We compared the protein concentration of 25 body cavity fluids as determined by 2 Goldberg type hand-held refractometers with results obtained by the biuret method. Published charts converting refraction (r) to protein concentration were used to determine protein concentration in samples with protein <2.5 g/dL. Higher protein values were read directly from the instruments. The range of comparison was limited to ≥ 0.6 g/dL, the lowest concentration of the biuret method's standard curve. Twenty-one peritoneal fluid, 2 pleural fluid and 2 pericardial fluid samples from 16 horses, 5 cattle, 3 dogs, 2 llamas and 1 cat were tested. The results obtained by the two refractometers were closely and linearly related to biuret results (P<.001), with slopes by linear regression analysis close to 1, and correlation coefficients >0.977. Based on this study, the range for quantification of body cavity fluid protein concentration by refractometry can be extended below 2.5 g/dL, allowing for quantitative assessment of most clinical samples. 相似文献
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Miyagawa Y Tominaga Y Toda N Takemura N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(5):679-681
One of the most important functions of the kidney is to concentrate urine through the reabsorption of water. Urine specific gravity (USG) is used in routine tests of urine concentration and can be estimated using a refractometer. However, as the scale of Japanese refractometer is based on experimental data from healthy Japanese people, and human USG obtained by Japanese refractometers show higher values than that by refractometer produced in Europe or the U.S.A. The purpose of this study was to establish correction formulas for the USG of dogs and cats measured using Japanese refractometers. In this study, we found that Japanese refractometers overestimated USG in both dogs and cats. This study shows that the correlation formulas described in this study are useful for the accurate evaluation of USG. 相似文献
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Urine specific gravity (USG) is used clinically as a measure of urine concentration, and is routinely assessed by refractometry. A comparison between optical analogue and digital refractometers for evaluation of canine urine has not been reported. The aim of this study was to compare a digital and an optical analogue hand-held refractometer for the measurement of canine USG, and to assess correlation with urine osmolality. Prospective study. Free-catch urine samples were collected from 285 hospitalised adult dogs, and paired USG readings were obtained with a digital and an optical analogue refractometer. In 50 dogs, urine osmolality was also measured using a freezing point depression osmometer. There was a small but statistically significant difference between the two refractometers (P<0.001), with the optical analogue refractometer reading higher than the digital refractometer (mean difference 0.0006, sd 0.0012). Paired refractometer measurements varied by <0.002 in 91.5 per cent of cases. The optical analogue and digital refractometer readings showed excellent correlation with osmolality (r=0.980 and r=0.977, respectively, P<0.001 in both cases). Despite statistical significance, the difference between the two refractometers is unlikely to be clinically significant. Both instruments provide an accurate assessment of USG in dogs. 相似文献
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Wallace MM Jarvie BD Perkins NR Leslie KE 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(6):573-575
Monitoring the passive transfer of immunoglobulins from colostrums particularly in noncentrifuged samples can be useful for calf health management programs. The results of total solids refractometry from centrifuge and noncentrifuge harvested sources of serum were highly correlated (R2 = 0.95). Results from a digital and a hand-held refractometer were highly correlated (R2 = 0.96). 相似文献
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N E Pfeiffer T C McGuire R B Bendel J M Weikel 《American journal of veterinary research》1977,38(5):693-698
Laboratory methods designed to quantitate serum immunoglobulin were evaluated: single radial immunodiffusion, zinc sulfate turbidity, and serum electrophoresis. Estimation of immunoglobulin concentration in neonatle calves, using total protein measurements, was also evaluated. Single radial immunodiffusion proved useful for quantitation when either class or subclass information was needed. Zinc sulfate turbidity measurements gave accurate results for total immunoglobulin except when hemolysis was present in the sample. A correction factor for hemoglobin that will minimize errors resulting from hemolysis was presented. Serum electrophoresis was also found to be an accurate quantitation method. Large errors were encountered in attempting to estimate immunoglobulin on the basis of total protein. 相似文献
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选择中国大陆最早分离的H9N2亚型禽流感病毒(avian influenza virus,AIV)A/Chicken/Guangdong/SS/94(H9N2)(缩写为SS株)和1998年大流行时期分离的H9N2亚型AIVA/Chicken/Shanghai/F/98(H9N2)(缩写为F株)为研究对象,对其在SPF鸡体内的复制能力和传播途径特性比较后发现,F株在4周龄SPF鸡气管中的复制能力高于SS株,F株可以经气溶胶传播途径传播,SS株不能经气溶胶传播途径传播;利用反转录-聚合酶链反应(RT-PCR)方法获取F株和SS株的HA和NA基因的cDNA,序列分析得知,F株和SS株的HA和NA基因的同源性分别是96.6%和98.1%;HA基因的裂解位点氨基酸序列都是PARSSR↓GL,但有5个氨基酸的差异,即166位N(F)→D(SS)、198位A(F)→V(SS)、217位V(F)→I(SS)、335位G(F)→R(SS)、504位L(F)→S(SS);2株病毒的NA基因在63~65位都存在氨基酸缺失,但在NA基因红细胞吸附位点的氨基酸序列不同,分别是IKKDSRSG(F)和IKEDLRSG(SS)。F株和SS株的传播特性差异是否与其表面基因序列有关,有待进一步研究。 相似文献
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本文系统地回顾了近60年以来国内外乡土灌草青贮利用与牛羊健康饲喂的研究现状与进展。通过对已有研究文献的年度分布、研究内容与研究区域进行分析,结合饲草青贮技术领域、草食畜牧业领域以及社会发展的背景,将乡土灌草青贮与牛羊健康饲喂划分为3个阶段,20世纪50年代到80年代末期为萌芽阶段、20世纪80年代末期到21世纪初期为缓慢增长阶段、21世纪初期至今为快速增长阶段。其次,根据研究内容从理论研究、监测评价、技术示范、技术研发4个方面进行归纳总结,分析了各分支领域的主要成果与关键问题,提出下阶段应重点研究的内容,应加强对乡土灌草饲料生产与牛羊健康饲喂的研究,综合提高饲料能量与蛋白质的利用率,尽可能改善牛羊的能氮平衡,促进畜牧业和饲料加工业的发展。 相似文献
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为分析猪葡萄球菌脱落毒素EXHC和SHETA基因结构并预测其所编码蛋白的结构和功能,本试验根据GenBank已报道的猪葡萄球菌脱落毒素EXHC和SHETA基因序列分别设计了1对特异性引物,从猪葡萄球菌GDZC株中扩增获得EXHC和SHETA基因片段,大小分别为1007和971 bp。序列分析结果表明,EXHC基因与猪葡萄球菌丹麦分离株(GenBank登录号:AF515455)及猪源松鼠葡萄球菌河北分离株(GenBank登录号:JF755400)的核苷酸序列、氨基酸序列同源性均为100.0%,而与其他葡萄球菌脱落毒素基因的核苷酸、氨基酸序列同源性分别为3.3%~53.9%和7.9%~44.2%。SHETA基因与日本分离株(GenBank登录号:AB036768)的核苷酸序列同源性为96.2%,氨基酸序列同源性为98.4%,在保守区共有31个碱基发生突变。利用DNAStar软件对EXHC和SHETA蛋白结构进行分析,结果显示EXHC基因编码的蛋白为亲水性蛋白,SHETA基因编码蛋白为疏水性蛋白,抗原性较差。本研究从猪葡萄球菌GDZC株中成功扩增获得EXHC和SHETA基因片段并对其编码的蛋白结构进行了预测,证实了中国分离的猪葡萄球菌同时携带有EXHC和SHETA 2种毒素基因,为进一步研究猪葡萄球菌的致病机理及毒素之间的相互作用提供了理论依据。 相似文献
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通过观察狐貂自咬症疾病行为特点,分析发病原因,传播途径,及时诊断治疗,以提高狐,貉养殖的经济效益。 相似文献
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果洛州草地鼠虫害情况及防治措施 总被引：9，自引：0，他引：9
１９９６年的调查，果洛州草地鼠虫害面积约为２４７．６万ｈｍ２，因鼠害每年损失牧草约１５９８．７万ｔ，相当少养１０９５．０１万羊单位。近１０年来使用Ｃ型肉毒梭菌毒素灭治取得很大成绩，累计灭治面积达到１８３．１万ｈｍ２。 相似文献
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