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利用抗、染根肿病F_2群体构建大白菜AFLP遗传连锁图谱 总被引：5，自引：2，他引：3
以易感染根肿病的大白菜(Brassica campestris ssp.pekinensis)自交系94SK和抗根肿病的CR Shinki DH系为亲本,通过杂交获得F1,并选择一株F1植株进行自交,构建了F2作图群体.利用扩增出与根肿病抗性基因CRb连锁标记的17对AFLP引物组合Pst+GNN/Mse+CNN构建大白菜遗传连锁图谱.这些AFLP引物组合共扩增出322个亲本间表现出多态性的AFLP标记.应用其中6个与CRb基因紧密连锁的AFLP标记转化成的SCAR标记和清晰可见的大于100 bp的211个AFLP标记构建了一张包含179个标记位点、10个连锁群、覆盖长度为576 cM,平均图距3.3 cM的遗传图谱.抗根肿病基因CRb定位在第一条连锁群9 cM的范围内.研究表明,利用Pst/Mse Ⅰ和EcoR Ⅰ/Mse Ⅰ引物组合以及其他分子标记类型构建遗传连锁图谱,并有效减少遗传图谱中聚集和间隙现象的产生提供了一定的科学依据.同时,该图谱的构建对在基因组范围内通过分子标记辅助选择选育大白菜根肿病抗性品种奠定了基础. 相似文献
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一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发 总被引：1，自引：0，他引：1
为寻找与TuMV抗性基因紧密连锁的分子标记,选用高抗病毒病大白菜自交系91-112和高感病毒病自交系T12-19以及由二者为双亲构建的包含100个株系的DH群体为材料,通过SSR和InDel标记的遗传分析,在A09上定位了一个新的与大白菜苗期TuMV-C4抗性相关的主效QTL位点BrTuA09。在此基础上,针对该QTL位点所在的标记区间,根据作图群体双亲的重测序结果,设计合成27对引物,其中11个InDel在双亲间具有多态性,且条带单一、扩增稳定;连锁分析发现,11个InDel标记均被定位在A09连锁群上BrTuA09的置信区间。利用BC1群体进行标记验证发现,这些标记对高抗单株选择的准确率均达到78%以上,可应用于分子标记辅助选择,为大白菜TuMV抗病分子育种奠定了良好的基础。 相似文献
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ET-ISJ标记的开发及陆地棉遗传图谱构建 总被引：1，自引：0，他引：1
根据植物结构基因外显子拼接位点的保守序列,设计扩增外显子的ET-ISJ (exon targeted intron-exon splice junction)标记引物。利用1 280对ET-ISJ引物组合,在陆地棉品种渝棉1号和T586中,筛选获得69对多态性引物组合,占引物组合的5.4%。用多态性ET-ISJ引物组合检测(渝棉1号×T586)F2:7重组近交系群体,得到70个位点。以70个ET-ISJ标记位点与523个SSR、59个IT-ISJ、29个SRAP和8个形态标记进行连锁分析,构建的遗传连锁图谱包括59个连锁群和673个位点(68个ET-ISJ、510个SSR、58个IT-ISJ、29个SRAP和8个形态标记)。连锁图覆盖3 216.7 cM,占棉花基因组的72.3%,标记间平均长度为4.8 cM。68个ET-ISJ标记分布于20条染色体。研究表明ET-ISJ标记多态性较高、稳定性好,可有效用于棉花与其他植物遗传连锁图谱构建。 相似文献
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大白菜遗传图谱的构建及与染色体关联分析 总被引：5，自引：0，他引：5
为构建大白菜遗传图谱,并与国际上公认的白菜参照图谱相关联。以大白菜晚抽薹DH系Y177-12和早抽薹DH系Y195-93为亲本建立的183个DH系为作图群体,利用SRAP、SSR、AFLP、STS、ESTP和CAPS等多种分子标记和形态标记构建大白菜遗传图谱,通过参照图上的锚定标记确定连锁群,并用最新的连锁群命名法进行命名。构建了包含10个连锁群、233个标记位点的分子遗传图谱,其中包括145个SRAP标记、62个SSR标记、12个AFLP标记、6个STS标记、4个ESTP标记、3个形态标记和1个CAPS标记。图谱总长度1 036 cM,标记间的平均图距为4.4cM。通过其中的38个SSR,4个ESTP和2个STS标记将各个连锁群与国际上认可A1-A10的参照连锁群相关联。该图谱的构建将为不同图谱的比较与整合及重要农艺性状的基因定位奠定基础。 相似文献
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花生AFLP遗传图谱构建及青枯病抗性QTL分析 总被引：6，自引：0，他引：6
青枯病抗性分子标记能为花生抗青枯病育种提供辅助选择技术,以抗青枯病品种远杂9102与感病品种Chico杂交构建的重组自交系群体(RIL)为材料,用66对具多态性的引物(EcoR I/MseI引物组合35对,MluI/MseI引物组合14对,PstI/MseI引物组合17对)对其进行扩增,共检测到324个多态性位点。应用JoinMap(3.0软件对这些多态性位点进行遗传连锁分析,构建了一张栽培种花生的AFLP遗传连锁图。该图谱包含98个AFLP标记,涉及20个连锁群,覆盖总距离285 cM,标记间平均图距为2.90 cM。结合RIL群体的青枯病抗性鉴定结果,利用分析软件QTLNetwork(2.0共检测到与青枯病抗性相关的3个QTL(qBWr1、qBWr2和qBWr3)。其中,qBWr1和qBWr2均位于第4连锁群,qBWr3位于第14连锁群。这3个QTL形成2对具有加性×加性上位性互作效应的QTL区段(qBWr1/qBWr3和qBWr2/qBWr3),贡献率分别为12.81%和16.56%,共解释青枯病抗性总变异的21.62%。 相似文献
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基于EST-SSR的木薯分子标记遗传连锁图谱的构建 总被引：3，自引：2，他引：1
此实验以木薯推广品种‘KU50’为母本,‘SC124’为父本通过杂交得到包含240个单株的F1分离群体,利用300对EST-SSR引物,20对SSR和20对SRAP引物组合对亲本和部分群体株系进行多态性分子标记筛选,共获得具有多态性的引物110对。在此基础上,利用这110对多态性引物对该F1群体进行分子标记的多态性分析,共获得269个多态性标记。利用JoinMap 3.0软件对这269个多态性标记进行分组和遗传图谱构建,最后获得了一张包含140个标记的木薯分子遗传连锁图谱,其中EST-SSR标记111个,SSR标记22个,SRAP标记7个;共21个连锁群,其中连锁群1和3（LG1、LG3）上的标记位点最多（15个）,LG21标记位点最少（2个）。此遗传连锁图谱的总长度为1314.775 cM,单个连锁群最长为132.904 cM（LG3）,最短为0.431 cM（LG19）,标记间平均长度9.391 cM。 相似文献
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本研究利用马尾松单株160粒种子的胚乳（大配子体）和235对微卫星（SSR）引物构建马尾松遗传图谱。经过筛选得到30对具有多态性的引物,产生97个分离位点,平均1.62位点/引物,偏分离率为11.34%。利用FsLinkageMap2.0软件对其进行连锁关系分析,74个标记分布在11个连锁群,有23个标记未连锁到任何连锁群上。该图谱覆盖的基因组总长度为927.91cM,最大连锁群图距为359.89cM,最小连锁群图距为20.44cM。平均每个连锁群的长度为84.36cM,标记间最大间距为28.2cM,最小间距为0,标记平均距离为12.54cM。估算的马尾松基因组大小为2541.21cM,基因组覆盖度为36.5%。利用马尾松大配子体构建该树种较饱和的遗传图谱,大约需要超过1250个标记,并应积极尝试AFLP和SRAP等标记;群体数目应超过200个;同时应开发适于半同胞群体的作图软件。 相似文献
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甘蓝型油菜SRAP、SSR、AFLP和TRAP标记遗传图谱构建 总被引：27，自引：0，他引：27
以黄籽GH06为母本、黑籽P174为父本杂交得到的第6代重组自交系188个株系为作图群体,通过SRAP、SSR、AFLP和TRAP四种分子标记对该群体进行遗传连锁分析,构建了一张包含20个连锁群、300个标记位点的甘蓝型油菜分子遗传图谱(LOD≥3.0),包括202个SRAP标记、65个SSR标记、23个AFLP标记和10个TRAP标记。图谱总长度1273.7cM,标记间平均距离为4.25cM。连锁群上的标记数在4～56个之间,连锁群长度变动在37.1～109.2cM之间,群内平均图距在1.80～14.20cM之间。LG1包含的标记最多,有56个;标记最少的连锁群(LG9、LG18、LG20)只有4个。LG13的平均图距最大,为14.20cM;LG6的平均图距最小,仅为1.80cM。在整个图谱上,存在图距大于20cM的空隙6个。本研究首次将SRAP及TRAP标记用于甘蓝型油菜遗传图谱的构建,结果表明,SRAP及TRAP标记在甘蓝型油菜遗传图谱的构建上是一种良好的标记系统。 相似文献
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《分子植物育种》2017,(1)
本研究以海岛棉品种新海3号及吉扎82为亲本构建的190个F2:3家系为材料,利用SSR分子标记构建了新疆海岛棉的遗传连锁图谱,为海岛棉分子标记辅助选育提供帮助。其主要研究如下:用从4 886对棉花SSR引物中筛选获得的双亲间多态性明显且重复性好的107对SSR引物对海岛棉(新海3号×吉扎82)F2群体进行基因型鉴定,共得120个稳定的SSR多态性位点,多态性频率为2.4%。初步构建了一个包含22个连锁群,74个标记,总长893 c M,覆盖海岛棉基因组20.10%的分子标记遗传连锁图谱。该图谱标记平均间距12.06 c M,最大图距54 c M,最小为1 c M,平均每个连锁群的标记数是3.4个。 相似文献
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David Okeh Igwe Celestine Azubuike Afiukwa 《Journal of Crop Science and Biotechnology》2017,20(4):263-278
Assessment of genetic diversity of Bambara groundnut (Vigna subterranea (L.) Verdcourt) accessions from Nigeria using informative molecular markers has become imperative for their genetic improvement and conservation. Comparative analysis of 30 V. subterranea from different locations in Nigeria was investigated using Directed Amplified Minisatellite DNA (DAMD) and Start codon targeted (SCoT) markers. The DNA was extracted using CTAB method for amplification. Both markers resolved the accessions into eight major groups using dendrograms and six clusters by principal component analysis. Alleles of 25 and 53 were obtained with DAMD and SCoT, respectively. Mean alleles, gene diversity, and polymorphic information content detected with DAMD were 10.2000, 0.6950, and 0.6600, while SCoT yielded 16.200, 0.847, and 0.836, respectively. Polymorphic loci were 130 and 142 in DAMD and SCoT, respectively. Both markers produced high polymorphism of 60.00-80% and 40.33-96.67% in DAMD and SCoT, respectively. Effective alleles (Ne) in both markers (DAMD: 1.1828-1.5927; SCoT: 1.1830-1.6779) were high. The Nei’s value (H) ranged from 0.1959-0.3238 in DAMD and 0.1533-0.3782 in SCoT. The Shannon’s information index (I) obtained from DAMD and SCoT were 0.3281-0.4635 and 0.1420-0.5557, respectively. Total gene diversity (Ht), diversity within population (Hs), coefficient of gene differentiation (Gst), and estimate of gene flow (Nm) from DAMD were 0.3304, 0.2673, 0.1907, and 2.1215, while SCoT had 0.3927, 0.3163, 0.1945, and 2.0713, respectively. Our study demonstrated that SCoT markers are more competent than DAMD and should be integrated in the exploration of genetic diversity and selection of unique accessions for improvement and utilization of V. subterranea. 相似文献
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Genetic study on important traits of tea is difficult because of its self-incompatibility in nature. Moreover, development of a new variety usually needs more than 20 years, since it takes many years from seedling to matured plants for trait investigation. Genetic map is an essential tool for genetic study and breeding. In this study, we have developed an integrated genetic map of tea (Camellia sinensis) using a segregating F1 population derived from a cross between two commercial cultivars (‘TTES 19’ and ‘TTES 8’). A total of 574 polymorphic markers (including SSR, CAPS, STS, AFLP, ISSR and RAPD), 69 markers with highly significant levels of segregation distortion (P < 0.001) (12.0 %) were excluded from further analyses. Of the 505 mapped markers, there were 265 paternal markers (52.5 %), 163 maternal markers (32.3 %), 65 doubly heterozygous dominant markers (12.9 %), and 12 co-dominant markers (2.4 %). The co-dominant markers and doubly heterozygous dominant markers were used as bridge loci for the integration of the paternal and maternal maps. The integrated map comprised 367 linked markers, including 36 SSR, 3 CAPS, 1 STS, 250 AFLP, 13 ISSR and 64 RAPD that were assigned to 18 linkage groups. The linkage groups represented a total map length of 4482.9 cM with a map density of 12.2 cM. This genetic map has the highest genetic coverage so far, which could be applied to comparative mapping, QTL mapping and marker assisted selection in the future. 相似文献
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YM型小麦温敏雄性不育系的不育基因被定位在1Bs染色体片段上, 但已发现的相邻分子标记与该基因的遗传距离较大, 达10 cM以上。为寻找与该基因连锁更紧密的分子标记, 以YM型温敏雄性不育系ATM3314与恢复系中国春杂交的F2代200株为作图群体, 从1Bs的22个SSR引物中筛选出5个在亲本和F2代中分离的SSR引物, 构建了1个包含5个标记的1Bs局部遗传连锁图谱。结合F2代个体的育性调查, 采用复合区间作图法在YM型温敏雄性不育系的1Bs染色体上检测到不育基因的1个主效QTLrfv1-1和1个微效QTLrfv1-2。rfv1-1位于SSR标记Xgwm18和Xwmc406之间, 与两标记的遗传距离分别为6.0 cM和4.6 cM, LOD值为8.80, 加性效应23.87, 显性效应10.44, 可解释表型变异的23.91%; rfv1-2位于Xwmc406和Xbarc8之间, 与两标记的遗传距离分别为4.0 cM和3.4 cM, LOD值为3.10, 加性效应17.59, 显性效应5.99, 可解释表型变异的7.78%。本研究初步定位了YM型小麦温敏雄性不育系1Bs染色体片段上不育基因的QTL, 为进一步准确定位该基因奠定了基础。 相似文献
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This work aimed to proceed molecular characterization of seven banana accessions (Borneo, Grand Naine, 1304-06, 4249-05, 0337-02,
0323-03 and 4279-06) resistance to the nematode Radopholus similis. These accessions were selected taking in account the reproduction factor (RF) among 26 banana genotypes from a working collection
belonging to Embrapa Mandioca e Fruticultura Tropical. The genomic DNA of the seven accessions was extracted, and 36 decamere
primers had been used to obtain RAPD markers. The resulting markers were converted into a matrix of binary data. From that
matrix the genetic distance between the accessions were estimated, for further clustering and graphic dispersion analyses.
From a total of 521 RAPD markers generated, 420 (81%) were polymorphic, including 140 (27%) potentially promising for application
on works related to genetic mapping of the resistance to R. similis. OPE-15, OPH-17, and OPG-09 were the primers that contributed to the highest number of bands promising for genetic mapping
of resistance (12, 8, and 8, respectively). The genetic distances between accessions ranged from 0.106 to 0.455, with the
longest one observed between cv. Borneo and the genotype 4279-06, considered as highly susceptible and resistant, respectively,
to the nematode according to the RF. The graphic dispersion distinguished three groups of accessions, and most of resistant
genotypes clustered together in the same group. The most contrastant genotypes for resistance (Borneo and 4249-05) were separated
by a genetic distance of 0.374, and possessed a total of 114 polymorphic bands promising for genetic mapping of resistance.
In addition, the results of pathogenicity tests were congruent with those obtained by RAPD analyses. 相似文献
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韭菜栽培品种遗传多样性的ISSR和RAPD研究 《中国农学通报》2005,21(4):44-44
利用RAPD(Random Amplified Polymorphic DNA)和ISSR(Inter-simple Sequence Repeat)两种分子标记技术对20份韭菜栽培品种进行了遗传多样性研究。结果表明，筛选后选用的12个ISSR引物和15个RAPD引物分析分别产生了258和101条扩增产物带，其中多态性条带(即20个韭菜品种中一个或多个但不是全部具有的带)分别为132和40条，分别占总数的51.2%和39.6%。，也就是说12个ISSR引物和15个SSR引物对韭菜不同品种的扩增可分别产生51 相似文献
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A pepper (Capsicum annuum) inbred line ‘BJ0747-1-3-1-1’ was found to exhibit resistance to a cucumber mosaic virus (CMV) local isolate (CMV-HB). In order to exploit the genetic mechanism of CMV resistance in this pepper line, inheritance and genetic linkages of CMV resistance were studied. The CMV-resistant ‘BJ0747-1-3-1-1’ (P1) was crossed to the susceptible line ‘XJ0630-2-1-2-1-1’ (P2), and the F2 and back-cross populations (B1 and B2) were generated for segregation analysis following a mixed inheritance model. Analysis of the segregation data revealed that CMV resistance in ‘BJ0747-1-3-1-1’ is controlled by two partially additive-dominant major genes and additive-dominant polygenes. Analysis of the results from two growth seasons also identified two stable and major QTLs for CMV resistance that collectively explained 55 % of the trait variation. One of the two major QTLs was found on linkage group 8 (LG8) between markers TS52 and HpmsE1-43, and accounted for 37.7–43.5 % of the variation in the two inoculation experiments. The other QTL on LG4 between markers UBC843 and S74 was found to be responsible for 10.7–11.2 % of the trait variation. QTLs with minor effects on CMV resistance were also identified. This work provides an example for genetic analysis and QTL mapping of other disease-resistance genes in pepper and the findings should be useful for molecular marker-assisted breeding programs that aim at developing disease-resistant cultivars in peppers. 相似文献
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Jorge Luis Fuentes Fabio Escobar Alba Alvarez Geraldo Gallego Miriam Cristina Duque Mirle Ferrer Juan Enrrique Deus Joe M. Tohme 《Euphytica》1999,109(2):107-115
A survey of the genetic diversity among the major cuban rice cultivars was conducted using isozyme, RAPD and AFLP markers.
Polymorphisms were detected for esterases, peroxidases, alcohol dehydrogenases and polyphenoloxidases systems; 21 RAPD primers
and four AFLP primer combinations. Heterozygosity arithmetic mean value (Hav(p)), the effective multiplex ratio (EMR) and the marker index (MI), were calculated for isozyme, RAPD and AFLP markers. The
mean value of genetic similarity among the different varieties was 0.92 for isozyme, 0.73 for RAPD and 0.58 for AFLP analyses.
Thus, AFLP were able to detect polymorphisms with higher efficiency than RAPD (+15%) and isozyme (+34%). Data from the isozyme,
RAPD and AFLP analyses were used to compute matrices of genetic similarities. The efficiency of the UPGMA for the estimation
of genetic relatedness among varieties was supported by cophenetic correlation coefficients. The resulting values indicated
that the distortion level for the estimated similarities was minimal. The correlation coefficients obtained by the Mantel
matrix correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated
values of genetic relationship given for isozyme and RAPD markers (r = 0.89), as well as for AFLP and RAPD markers (r = 0.82)
were properly related. However, AFLP and isozyme data showed only moderate correlation (r = 0.63). Although the genetic variability
found among the different cultivars was low, both RAPD and AFLP markers proved to be efficient tools in assessing the genetic
diversity of rice genotypes.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
19.
海岛棉(Gossypium barbadense)是世界上最重要的栽培棉种之一。海岛棉纤维品质优良,是优质棉的重要产源。为了研究海岛棉的遗传多样性,为海岛棉育种提供参考依据,从海岛棉遗传标准系中分离基因组来源的微卫星标记用于海岛棉遗传评价。采用两种方法分离微卫星标记,一是用ISSR (inter simple sequence repeat) 引物扩增Pima3-79,克隆测序后从中开发微卫星标记;二是利用简并引物扩增Pima3-79,克隆测序后从中开发微卫星标记。共挑选1 447个克隆,筛选出239个独立克隆。测序后得到214个单一序列,其中包含微卫星并可用于引物设计的序列70个,获得86对引物。86对引物用于扩增56个海岛棉材料和4个陆地棉材料,16对引物没有扩增,43对引物在所有材料中没有多态性;27对引物在海岛棉和陆地棉之间有多态性,19对引物在海岛棉中表现多态性。利用Jaccard相似系数和UPGMA方法进行聚类分析可以明显区分陆地棉和海岛棉,并且将海岛棉分为4类。14对引物在BC1群体中表现多态性,产生14个位点。9个位点整合到BC1连锁图的7个染色体上,4个位于A亚基因组,5个位于D亚基因组。海岛棉微卫星标记扩展了棉花微卫星标记,有助于海岛棉遗传多样性的研究,有利于棉花遗传图谱的进一步丰富。 相似文献
20.
Guangjie Liu John L. Bernhardt Melissa H. Jia Yeshi A. Wamishe Yulin Jia 《Euphytica》2008,159(1-2):73-82
Recombinant inbred line (RIL) populations of rice are useful genetic sources for map-based cloning of agronomically important
genes. Zhe733 is a high-yielding indica cultivar from China conferring resistance to rice blast (RB), rice water weevil (RWW) and straighthead; whereas Kaybonnet
low-phytic acid 1-1 (KBNTlpa) is a mutant of a tropical japonica cultivar from the US containing low-phytic acid with average yield, and is susceptible to some RB races, RWW, and straighthead.
A 355 RIL F10−11 population derived from the cross of KBNTlpa × Zhe733 was recently released. Simple sequence repeat (SSR) markers were used to evaluate 269 RILs of this population. A
total of 107 polymorphic markers were mapped on all rice chromosomes representing a total of 1,016.3 cM of genetic distance.
Two hundred and thirty-five KBNTlpa × Zhe733 RILs (KZRILs) were clustered into seven groups based on allele frequencies of SSR markers. Twenty-three markers
(21.1%) on chromosomes 3, 6, 7, 9, and 11 were found to favor Zhe733 (χ
2 = 16.8−189.7 and P < 0.01) and five markers (4.6%) on chromosome 1 and 6 were found to favor KBNTlpa (χ
2 = 18.5−46.6 and P < 0.01). Marker segregations were observed to be normal for both parents except 26 (10.2%) KZRILs were found to skew toward
Zhe733 (χ
2 > 15.7 and P < 0.01). Furthermore, the average frequencies of heterozygosity and non-parental alleles per KZRIL were 1.3% (0.0−38.9%)
and 0.4% (0.0−15.0%), respectively. Thirteen heterozygous KZRILs were found at more than five markers loci and nine KZRILs
were found with more than five non-parental alleles representing 5.1 and 3.5% of 255 KZRILs. Overall, this KZRIL population
is a good population with relatively low frequencies of heterozygosity and non-parental alleles, and with relatively low percentages
of skewed markers and skewed KZRILs. The profiles of these SSR markers should facilitate molecular tagging critical genes
controlling yield, RB, RWW, and straighthead resistance. 相似文献