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‘天汪1号’是‘红星’苹果的短枝型芽变．1980年发现于天水市秦州区（原天水县）杏湾村果园．1981年春季高接并繁育小苗，经多年多点栽培观察、品种比较试验、区域试验及生产试栽。确认其比‘红星’树体相对矮小，结果早．丰产；成熟期提前．短枝性状稳定．尤其在山地的适应性好于新红星等元帅系短枝型品种．2003年通过国家林业局林木品种审定委员会审定。现在天水果区栽培面积达12000公顷，并在甘肃、陕西、河北、辽宁、山东等地引种栽培。 相似文献
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对元帅系短枝型品种‘新红星’、‘玫瑰红’、‘岱红’和普通型“红星’的果形指数(L/D)变化动态和影响L/D斩因素进行观察研究表明,这几个短技品种的L/D变化动态与普通型‘红星’基本相似。花期喷布400～1400倍的普洛马林能显著提高L/D,最适喷布时期为盛花期至落花期。结果量、结果枝类型、花朵开放的早晚、花朵着生部位等对果型指数都有一定的影响。提高贮藏营养水平和花芽质量的措施都能改进果形。 相似文献
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苹果短枝型品种矮化早果机制研究：营养特性 总被引：3，自引：0，他引：3
比较了在同一立地条件下生长年龄、砧木完全一致的苹果短枝型品种和普通型品种的营养特性。结果表明：（１）短枝型品种地上部的Ｎ素营养、碳素营养明显高于普通型品种，地下部根系的营养含量相反；（２）短枝型品种耐肥性较高；（３）两类品种对铵态氮和硝态氮肥的田间反应差异。 相似文献
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富士芽变系品种花粉形态初探 总被引：3，自引：0，他引：3
用扫描电镜对富士和长富2号、福岛短枝富士、早熟富士、望山红等芽变系品种的花粉进行了形态观察。结果表明: 这些富士芽变系品种的花粉粒侧面观为长椭圆形, 极面观为三角形; 具3拟孔沟;花粉表面为条状纹饰, 有穿孔。‘福岛短枝富士’花粉粒大, 极轴长最长, 为42.25 μm; 赤道轴长为25.08μm, 略短于‘早熟富士’, 明显长于其它3个品种; ‘富士’的P /E值最大, 为1.75, 其次是‘长富2号’和‘望山红’, 分别为1.73和1.71, 三者明显大于‘早熟富士’的P /E值。P /E值可作为芽变品种鉴定的重要指标。每个品种的花粉纹饰各有特点, 可通过花粉的扫描电镜观察来鉴别以上的富士芽变系品种。 相似文献
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元帅系短枝型品种的适采期自１９８５年以来，在我市大量定植的新红星、首红等一批元帅系短枝型品种均已挂果。早实、丰产、经济效益高是元帅、红星等普通型品种不可比拟的。但从近年当地的习惯采收情况看，大多果商于８月６日就开始收购果实，直至中秋节前５天为抢收高峰... 相似文献
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苹果短枝型品种的施肥技术一、短枝型品种的需肥特点（１）短枝型品种在富含有机质的沙壤土中生长，结果最好。土壤有机质含量应达到２％－３％才能适应丰产栽培的需求。（２）由于短枝型品种结果早，易丰产，因此对肥水条件要求较普通型品种高。另外对微量元素的需求量也... 相似文献
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抗寒优质苹果新品种‘短枝寒富’‘短枝寒富’是由沈阳农业大学教授李怀玉等用东光×富士杂交选育出的抗寒优质苹果新品种，已经通过省级审定。该品种集双亲的优良性状于一身，以短果枝结果为主，还兼有腋花芽结果的特性，花序坐果率达８２．５％，花朵坐果率达４６．２％... 相似文献
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为探索梨树砧木的矮生机制,以矮生型梨砧木‘PY-9’、‘BA-29’、‘OHF-87’、"东7-7"、"中矮Ⅱ号"、"FOX11"等为试材,以普通型梨砧木杜梨(Pyrus betulifolia)、豆梨(P.calleryana)、砂梨(P.pyrifolia)、秋子梨(P.ussuriensis)、木梨(P.xerophila)、川梨(P.pashia)、褐梨(P.phaeocarpa)、杏叶梨(P.armeniacaefolia)、柳叶梨(P.salicfolia)等为对照,采用组织离析、显微照相与生物学统计方法,对1年生枝条导管分子的形态和大小进行了观察。结果表明:普通型和矮生型梨砧木的导管分子形态多为圆柱形,均属于孔纹导管;其矮生型导管分子的穿孔板类型、有尾情况、端壁倾斜状况、端尾长度与普通型无明显差异;矮生型导管分子平均长度在275.1~300.8μm,平均直径在26.1~29.9μm;普通型梨砧木枝条导管分子平均长度在331.6~399.3μm,平均直径在31.2~36.0μm;矮生型梨砧木导管分子长度与直径显著小于普通型梨砧木。以上结果表明,矮生型梨砧木表现出小导管性,这可能是矮生型梨砧木致矮的细胞学原因之一。 相似文献
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Asthma is a common respiratory disease. In recent years, with the aggravation of environmental pollution, the morbidity and mortality of asthma have shown a significant upward trend. Recent literature provides evidence that transient receptor potential cation channel subfamily V member 1 (TRPV1) has both promoting and inhibitory effects on asthma and plays an important role in the development of asthma. Therefore, it is necessary to identify of TRPV1 receptor in asthma, in order to clarify the relationship between TRPV1 receptor and asthma, and to provide theoretical basis for drug research. 相似文献
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Serpin peptidase inhibitor clade E member 2 (SerpinE2), a member of serine peptidase inhibitor super family, regulates multiple serine proteases. Extracellular matrix (ECM) plays an important role in stem cell differentiation, signal transduction, tumor metastasis, osteoarthritis physiological and pathological processes and so on. SerpinE2 regulates the protein of extracellular matrix by serine protease system and matrix metalloproteinase (MMP) system. In this paper, we elaborate the effect of SerpinE2 on metabolism of extracellular matrix, and it will provide new ideas to find new therapeutic targets. 相似文献
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AIM:To investigate the effects of propofol (P) on the inflammatory response of microglia induced by lipopolysaccharide (LPS) and the mechanisms. METHODS:Mouse microglia BV2 cells were treated with LPS at 100 μg/L to establish a neuroinflammatory injury model. The BV2 cells were divided into 4 groups:control group (C group), model group (L group), L+P group and LPS+AMG517 group (L+A group). The level of tumor necrosis factor-α (TNF-α) in the cell culture supernatant was measured by ELISA. The mRNA expression of transient receptor potential cation channel subfamily V member 1 (TRPV1) was detected by real-time PCR. The protein levels of TRPV1, TNF-α, interleukin-1β (IL-1β), interleukin-6 (IL-6) and phosphorylated calcium/calmodulin-dependent protein kinase Ⅱ (p-CaMKⅡ) were determined by Western blot. The content of free Ca2+ in the microglia BV2 cells was detected by Fluo-3 AM assay. RESULTS:Compared with C group, the level of TNF-α was significantly increased in L group (P<0.01), but that in P group was not changed. Compared with L group, the level of TNF-α was significantly lower than that in L+P group within 4 h (P<0.01). Compared with C group, the mRNA expression of TRPV1 was significantly increased in L group (P<0.01). Compared with L group, the mRNA expression of TRPV1 was significantly down-regulated in L+P group (P<0.01).Compared with L group, the protein levels of TNF-α, IL-1β, IL-6 and p-CaMKⅡ and intracellular Ca2+ concentration were significantly lower than those in L+P group and L+A group (P<0.01). CONCLUSION:Propofol inhibits the inflammatory response of microglia by reducing the expression of TNF-α, IL-1 and IL-6, which may be related to the down-regulation of TRPV1 and p-CaMKⅡ and the reduction of intracellular Ca2+ concentration. 相似文献
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MA Hong-ming HUANG Yuan-shun HUANG Miao-ling ZHANG Miao CHEN Yang HUANG Zhi-hong CUI Miao LIANG Wan-ling LIU Sheng-ming CAI Xing-dong 《园艺学报》2000,36(9):1680-1689
AIM To investigate the significance of transient receptor potential cation channel subfamily M member 8 （TRPM8） protein expression in lung adenocarcinoma. METHODS The tumor samples from 112 cases of patients with lung adenocarcinoma were collected in our hospital， and 4~5 years of follow-up was conducted. The protein expression of TRPM8 was analyzed by immunohistochemical staining， and the correlations between the TRPM8 protein expression and the clinical characteristics including prognosis of the patients with lung adenocarcinoma were investigated. After TRPM8 protein expression was up-regulated in A549 lung adenocarcinoma cells by lentiviral infection， the proliferation of A549 cells was analyzed by CCK-8 assay and colony formation assay， the cell cycle and apoptosis were analyzed by flow cytometry， and the migration and invasion abilities of the cells were measured by scratch experiment and Transwell assay. The TRPM8 protein expression was stably up-regulated in H1299 cells by lentiviral infection， and then the left and right buttocks of the immunodeficient mice were subcutaneously injected with empty vector control cells and TRPM8-overexpressing cells， respectively. The effects of TRPM8 on the growth of H1299 cell-derived xenograft tumor in immunodeficient mice were evaluated. RESULTS The 4~5-year survival rate in the patients with high TRPM8 protein expression was significantly higher than that in the patients with low expression of TRPM8 protein （P =0.017）. The tumor maximum diameter in the patients with high TRPM8 protein expression was significantly smaller than that in the patients with low TRPM8 protein expression （P =0.028）. The viability， the number of colonies and the migration and invasion abilities of TRPM8-overexpressing A549 cells were significantly decreased as compared with empty vector and parental cells （P< 0.01）. The results of flow cytometry analysis showed that the proportion of A549 cells at S stage was significantly increased in TRPM8 overexpression group as compared with empty vector group （P< 0.01）. The growth rate and the weight of TRPM8-overexpressing H1299 cell-derived xenograft tumor in immunodeficient mice were significantly lower than those in empty vector group （P <0.01）. CONCLUSION TPRM8 is a tumor suppressor in lung adenocarcinoma， and low expression of TRPM8 protein was a poor prognositic indicator of patients with lung adenocarcinoma. 相似文献
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FENG Bao-hong WU Jun ZHANG Yan-xia ZHU Ge-li GONG Xue-min BI Zhi-min HU Man-li 《园艺学报》2020,36(6):1110-1114
AIM To investigate the effects of uric acid on mitochondrial damage and the expression of phosphoglycerate mutase family member 5 (Pgam5) and dynamin-related protein 1 (Drp1) in rat kidney cells. METHODS Normal rat kidney NRK-52E cells were treated with uric acid at 0.6 mmol/L. The cell viability was measured by MTT assay. The cell morphological change was observed by Hoechst 33258 staining. The apoptosis of the cells was analyzed by flow cytometry. The morphological structure of mitochondria was observed under transmission electron microscope. The expression of Pgam5 and Drp1 was examined by immunofluorescence staining. The mRNA expression of Pgam5 in mitochondria was detected by RT-qPCR. The protein expression of Pgam5 and Drp1 in the cytoplasmic matrix was determined by Western blot. RESULTS Uric acid significantly decreased the viability of NRK-52E cells, and significantly increased the apoptotic rate of the cells (P <0.01). Mitochondrial swelling, vacuolation and cristal rupture were observed after the NRK-52E cells were treated with uric acid, and the mRNA expression of Pgam5 in mitochondria was decreased significantly, while the protein expression of Pgam5 and Drp1 in the cytoplasmic matrix was increased significantly (P <0.01). CONCLUSION Uric acid intervention destroys the mitochondrial structure of rat renal cells, up-regulates the expression of Pgam5 and Drp1 in the cytoplasmic matrix, and induces apoptosis of the cells. 相似文献
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ZHANG Ya-hui WANG Xiong WU Sheng-ying PENG Ji-xia WU Fu-yun KE Jing ZHANG Peng ZHANG Qiu-fang LV Yan-xia 《园艺学报》2017,33(9):1581-1586
AIM: To determine the role of nuclear receptor subfamily 6, group A, member 1 (NR6A1) in vascular smooth muscle cell (VSMC) apoptosis.METHODS: NR6A1 protein was over-expressed in the VSMCs by infection of adenovirus. The effect of NR6A1 on the viability of VSMCs was measured by MTT assay. DAPI staining, TUNEL staining and caspase activity assay were conducted. DNA microarray was used to quickly screen the target genes of NR6A1. The effect of receptor-interacting serine/threonine-protein kinase 3 (RIPK3) silencing on NR6A1-induced apoptosis of the VSMCs was further analyzed.RESULTS: Adenovirus-mediated over-expression of NR6A1 induced the apoptosis of VSMCs. The RIPK3 gene expression was up-regulated by NR6A1 over-expression in the VSMCs. NR6A1-induced VSMC apoptosis was inhibited by RIPK3 silencing.CONCLUSION: NR6A1 promotes VSMC apoptosis by up-regulating the RIPK3 gene expression. 相似文献
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Correlation between expression of ALDH1/ABCG2 and microvessel formation in epithelial ovarian cancer
AIM: To elucidate the correlation between the expression of aldehyde dehydrogenase 1 (ALDH1)/ATP-binding cassette subfaminly G member 2 (ABCG2) and microvessel density (MVD) in epithelial ovarian cancer (EOC).METHODS: In 198 specimens of EOC and 60 specimens of ovarian benign epithelial tumor tissues, the protein expression of ALDH1/ABCG2 and CD105 (microvessel marker) was detected by immunohistochemical staining.RESULTS: The positive rates of ALDH1 and ABCG2 in the EOC were 64.1% and 61.6%, respectively, while the positive rates in benign epithelial tumor tissues were 8.3% and 6.7%, respectively, and there were significant differences between them (P<0.05). In EOC and benign epithelial tumor tissues, the MVD were 22.6±9.7 and 5.03±3.35, respectively, and the difference was also significant (P<0.05). The expression of ALDH1 and ABCG2 in EOC was significantly related to differentiation, FIGO stage,and abdominal organ and lymph node metastasis (P<0.05). MVD had correlation with differentiation, FIGO stage, ascite, and abdominal organ and lymph node metastasis (P<0.05). MVD had positive correlation with the expression of ALDH1 and ABCG2 (P<0.01). There was also a positive correlation between the expression of ALDH1 and ABCG2 (P<0.01). Over-expression of ALDH1/ABCG2 and MVD≥23 were related to the poor prognosis. The survival rates in ALDH1/ABCG2 positive and MVD≥23 groups were significantly lower than those in ALDH1/ABCG2 negative and MVD<23 groups (P<0.05). The FIGO stage, the expression of ALDH1/ABCG2 and MVD were indepen-dent prognosis factors of EOC (P<0.05).CONCLUSION: The results suggest that the expression of ALDH1/ABCG2 and MVD in EOC are related to differentiation, lymph node metastasis, clinical stage and prognosis. Combined detection of these indexes may play an important role in predicting the progression and prognosis of EOC. 相似文献
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AIM:To study the effects of microRNA-105(miR-105) on the cell proliferation, migration and invasion abilities of non-small-cell lung cancer (NSCLC) H460 cells, and further to explore its mechanism. METHODS:The expression of miR-105 and kinesin family member C1 (KIFC1) mRNA in the NSCLC tissues and adjacent tissues and cells was detected by RT-qPCR. The protein expression of KIFC1 in the NSCLC tissues, adjacent normal tissues and cells was determined by Western blot. The H460 cells were divided into miR-105 group (transfection with miR-105 mimics), miR-negative control (NC) group (transfection with miR-NC), inhibitor-NC group (transfection with NC of inhibitor), inhibitor-miR-105 group (transfection with miR-105 inhibitor), si-NC group (transfection with NC siRNA), si-KIFC1 group (transfection with KIFC1 siRNA), miR-105+vector group (miR-105 mimics and pcDNA 3.1 co-transfection) and miR-105+KIFC1 group (miR-105 mimics and pcDNA 3.1-KIFC1 co-transfection). The cell proliferation was measured by MTT assay and colony formation assay. The migration and invasion abilities were detected by Transwell methods. The relative luciferase acitivity was evaluated by double luciferase reporter assay. RESULTS:Compared with the adjacent tissues, the expression of miR-105 was significantly decreased and the expression of KIFC1 was significantly increased in NSCLC tissues (P<0.05). Compared with human normal embryonic lung fibroblasts MRC-5, the expression of miR-105 in the H460 cells was significantly decreased, and the expression of KIFC1 was significantly increased (P<0.05). miR-105 inhibited the relative luciferase activity of H460 cells with wild-type KIFC1 and negatively regulated the protein expression of KIFC1. Over-expression of miR-105 and knockdown of KIFC1 expression significantly inhibited the proliferation, migration and invasion abilities of H460 cells. Over-expression of KIFC1 reversed the inhibitory effect of miR-105 on the cell proliferation, migration and invasion abilities of H460 cells. CONCLUSION:miR-105 inhibits the proliferation, migration and invasion abilities of NSCLC cells. The mechanism may be related to targeting and negatively regulating expression of KIFC1. 相似文献
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SHI Meng-ru NAN Chao XU Li-yan HAN Wen-wen XU Zi-qin LI Dong QIU Qiao-meng LU Zhong-qiu 《园艺学报》2013,29(12):2223-2228
AIM:To investigate the effects of transient receptor potential cation channel subfamily V member 1 (TRPV1) activation by capsaicin on the inflammation and its underlying mechanisms in lipopolysaccharide (LPS)-induced lung injury in mice. METHODS:A total of 108 specific pathogen-free male ICR mice were randomly divided into 6 groups: normal control group, capsaicin (CAP) control group, capsazepine (CAPZ) control group, endotoxemia group, CAP treatment group and CAPZ treatment group. LPS was intraperitoneally injected 30 min after the subcutaneous injection of CAP or CAPZ. After modeling, the levels of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), IL-10, substance P (SP) and calcitonin gene-related peptide (CGRP) in the lung were measured by ELISA. The expression of Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB) in the lung tissue was assessed by Western blotting. The pathological changes of the lung tissue were observed under light microscope. RESULTS:The expression of TNF-α, IL-6, IL-10 and NF-κB in the lung tissues at 3 h, 8 h and 16 h was dramatically higher in endotoxemia group than that in normal control group. Compared with endotoxemia group, the levels of TNF-α, IL-6 and nuclear NF-κB in CAP treatment group at 3 h, 8 h and 16 h were obviously decreased, but the level of IL-10 was increased. The changes of the factors mentioned above in CAPZ treatment group were absolutely adverse to those in CAP treatment group. The levels of SP and CGRP were significantly higher in endotoxemia group and CAP control group than those in normal control group, but those in CAPZ control group were lower. Compared with endotoxemia group, SP and CGRP were markedly increased in CAP treatment group and were obviously decreased in CAPZ treatment group. The level of TLR4 in endotoxemia group was distinctly higher than that in normal control group at 3 h, 8 h and 16 h. However, as compared with endotoxemia group, the expression of TLR4 in CAP treatment group and CAPZ treatment group didn’t change much. At 8 h and 16 h after modeling, the degree of lung damage was also decreased in CAP treatment group as compared with endotoxemia group, while that in CAPZ treatment group was aggravated. CONCLUSION: TRPV1 activation obviously inhibits the increase in TNF-α, IL-6 and NF-κB in the lung tissue of endotoxemia mice, and promotes the increase in the anti-inflammatory factor IL-10, as well as the levels of SP and CGRP, but has no effect on the expression of TLR4. 相似文献
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温州蜜柑花蕾数及第一二次生理落果后的果数与越冬叶片数量均呈极显著线性正相关。冬季落叶率在４０％以下者，着果率为２．７％～４．４％；落叶率在５０％以上者，着果率在１．４％左右；落叶率在７０％以上者，着果率在１％以下。越冬期间采用灌水、施有机肥、覆盖地膜等措施，均有较好的保叶作用。 相似文献