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《畜禽业》2015,(5)
为了解上海地区猪2型圆环病毒病的流行现状,于2010~2014年间开展了流行病学调查和分离毒株的基因型分析,结果表明发病场户构成比例中,规模猪场占62.1%,专业养猪户占37.9%;PCV2引发的临床病症中,PMWS比例最高,占58.6%;PDNS次之,占24.1%,其它病症占17.2%;经对不同季节发病情况的统计,冬季发病比例最高,达44.8%,夏季次之,占27.6%,春季和秋季则分别占17.2%和10.4%。经对29个PCV2感染病例的病原学检测结果分析,平均混合感染率达到53.1%。经对病原学阳性样本的基因型分析,以PCV-2b感染为主,所占比例为93.1%;其次为PCV-2a,占6.9%。 相似文献
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猪圆环病毒病研究进展 总被引：2，自引：0，他引：2
<正>猪圆环病毒(PCV)病是近几年新发现的一种猪的传染病,由猪圆环病毒(porcine circovirus,PCV)引起猪的一种多系统功能障碍性疾病。猪圆环病毒病临床上以新生仔猪先天性脑震颤和断奶仔猪多系统衰竭综合征(postw-eaning multisystemic wastingsyn-drome,PMWS)等为主要特征。该病原体已被确认为圆环病毒(PCV-2),研究表明,Ⅱ型猪圆环病毒(PCV-2)不仅是断奶仔猪多系统衰竭综合症(PMWS)的病原,而且能够引起多种猪病,主要有PMWS、猪皮炎及肾病综合征、猪增生性坏死性肺炎、猪呼吸与繁殖障碍综合征、猪细小病毒、传染性先天性震颤等均与PCV-2感染有重要关联。 相似文献
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根据JineBank中报道的猪圆环病毒II型(PCV-2)ORF2的核苷酸序列设计一对引物,以重庆梁平县某猪场疑似PMWS的断奶仔猪病料为模板扩增出了特异性的DNA片段,长度约为1150bp,与预期值相符,证实重庆地区猪群中有PCV-2的感染。为重庆地区猪圆环病毒的控制和防疫提供了理论依据。 相似文献
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猪圆环病毒2型(Porcine circovirus type 2,PCV2)可以引起猪生殖和呼吸道疾病、断奶仔猪综合征、皮炎和肾炎等，给全球养猪业造成了严重的经济损失。快速、精准的检测方法对该病毒的防控尤为重要。对近年来国内外已发表的PCV2快速检测方法的原理、特点及检测应用进行综述，主要介绍了基于PCR的检测方法(常规PCR、巢式PCR、荧光定量PCR、多重PCR、纳米PCR、数字PCR及其他基于PCR的方法)、核酸等温扩增技术(环介导等温扩增、重组酶介导等温核酸扩增、重组酶聚合酶扩增)、基因芯片、原位杂交、液相芯片系统以及免疫学检测方法(免疫层析技术、酶联免疫吸附试验、琼脂扩散试验、斑点测点法、表面等离子体共振技术)。此外，也对相关方法的未来发展方向进行了展望，以期为我国有效防控PCV2传播和感染提供科学而全面的参考。 相似文献
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<正>猪圆环病毒病(PCVD)是由圆环病毒2型(PCV-2)感染引起的免疫抑制性疾病,是一种新的传染性疾病。单独的圆环病毒2型感染并不会引起猪出现临床症状,病毒只在环境应激、饲养管理不当或其他病原侵袭的情况下才会导致临床发病。猪圆环病毒病是一系列多种不同临床疾病的总称,是指群体病或与PCV-2相关的疾病,包括断奶仔猪多系统衰竭综合征(PMWS)、猪呼吸道病综合征(PRDC)、猪皮炎肾病 相似文献
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广西某地发病猪场主要病原的检测与调查分析 总被引：3，自引：0，他引：3
于2006年9月至2007年7月间,对广西某市以发热、咳嗽、呼吸困难、皮肤及耳朵发绀和高发病率及高病死率等为主要临床特征的39个发病猪场所采集的53份病料进行了主要病原检测。检测结果显示,猪繁殖与呼吸综合征病毒(PRRSV)阳性30份,阳性率56.60%(30/53);猪2型圆环病毒(PCV2)阳性25份,阳性率47.17%(25/53);猪流感病毒(SIV)阳性21份,阳性率39.62%(21/53);猪瘟病毒(HCV)未检出阳性;其中可检出2种以上病原的有26份病料,占48.06%(26/53)。检测结果与调查分析表明,猪繁殖与呼吸综合征(PRRS)、猪2型圆环病毒(PCV2)和猪流感(SI)是导致该市猪场疾病发生流行的主要病种,而且PRRS、PCV2和SI常呈二重或多重合并感染,这为该市发病猪场针对性地做好疫病的防控提供了科学依据。 相似文献
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人们越来越多地在育肥猪的呼吸道疾病中诊断出与猪圆环病毒II型相关的肺炎。很多实验室诊断的呼吸系统综合征(PRDC)病例通常是多种病毒混合感染。在美国,2000年,猪繁殖与呼吸综合征病毒(PRRSV)占病例的42%,PCV2占22%,猪流感病毒(SIV)占19%,猪肺炎支原体占22%;我们国家现在的情况与此相似。本文介绍3个临床病例分别是PRRSV和PCV2共感染、PCV2和SIV共感染以及PCV2和猪肺炎支原体共感染。这就是今年国内的主要猪病形势,经常导致严重的临床疾病,唯一的组织学损伤是淋巴组织消退和气道纤维化,在这些损伤中大量的PCV2抗原表明PCV2在PRDC中起到重要的致病作用。去年夏季,江苏、安徽、江西、湖南、湖北、河南、山东等地的部分区域发生了以高热、呼吸困难和全身性出血为主的猪疫情。根据所收集的流行病学资料、实验室检测结果和相关动物实验,初步认为造成本次猪疫情发生的主要病原为多杀性巴氏杆菌(Pasteurella multocida)、猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus)以及猪2型圆环病毒(Porcine circovirus-2)感染,即3P综合征(3P Syndrome,以下称3P综合征)。这就是例证。PCV2感染相关的疾病经常与一种或多种病原体共发生。PCV2感染经常在PRDC暴发中出现,可能导致这些病例中损伤的严重性和持续性的原因。PCV2感染相关的损伤能够在急性呼吸系统疾病中发现,并不局限于消瘦症状。 相似文献
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通过了解大鲵虹彩病毒(Giant salamander iridovirus,GSIV)在EPC细胞接毒情况和PCR的实际检测情况,为大鲵的病毒检测提供必要判断依据和检测方法.通过细胞接毒和组织分离分别提取病毒质粒,进行普通PCR扩增,电泳分析,比较病毒细胞培养和组织直接提取结果.Gsiv病毒体外培养:经传代48 h的EPC细胞,25℃培养,12h后可观察到CPE出现,24 h病变明显,CPE达15％,48 h后CPE可达70％,72 h后细胞完全脱落;PCR检测:对3个样本分别从组织提取和经细胞扩增分离的病毒进行普通PCR扩增检测,经细胞培养扩增的3个样本全部呈阳性;经组织提取的3个样本,2个呈阳性,一个呈阴性;电泳观察,病毒的扩增产物在500 bp左右.在体外环境下GSIV是高病力的一种病毒,对EPC细胞在25℃时效应时间为3d,普通PCR经组织的检出率低,经细胞培养扩增可提高检出率但耗时久,不利于实际生产中快速、准确诊断疫情的需要. 相似文献
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鲤浮肿病(carp edema virus disease,CEVD)是在锦鲤中发现的一种传染性病毒病,近年来在全球传播迅速,对鲤养殖业造成严重损害。本研究设计CEV环介导等温扩增(loop-mediated isothermal amplification,LAMP)引物,进行特异性实验;选取套式PCR、荧光PCR和LAMP三种检测方法,组织10家实验室进行室间比对测试:制备含CEV病毒核酸的测试样品,评价其均匀性和稳定性,通过测试结果分析方法检出限。结果显示,套式PCR的核酸样品检测限可达6.8×10-10 g。 相似文献
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Jeffery KR Bateman K Bayley A Feist SW Hulland J Longshaw C Stone D Woolford G Way K 《Journal of fish diseases》2007,30(11):649-656
Haematopoietic necrosis virus [cyprinid herpesvirus 2 (CyHV-2)] was isolated during disease outbreaks in goldfish, Carassius auratus, at an ornamental fish retail site in southern England in 2004. Signs of disease included lethargy and inappetence and were first seen after water temperatures increased from 14-15 to 19-21 degrees C. External gross pathology included pale patches on the gills and skin and internally the spleen was enlarged, often with distinctive white nodules. The most prominent histopathological changes observed were necrotic lesions in the spleen and kidney and focal patches of necrosis in the gill lamellae. Necrotic cells often contained nuclei with marginated chromatin and pale intranuclear inclusions. Ultrastructural examination of the spleen tissue revealed typical herpesvirus-like particles measuring 100 nm in diameter. The virus was isolated from extracts of gill tissue in KF-1 cells at 20 degrees C and oligonucleotide primer sets were designed based on conserved gene sequences and used to amplify viral DNA by polymerase chain reaction (PCR). The PCR assays were then used to detect the virus in DNA extracted from tissues sampled during earlier disease investigations at the retail site owner's holding facility in 2002 and 2003 and stored at -70 degrees C since then. Polymerase gene-specific PCR amplification products obtained from tissue samples and from the virus isolated in cell culture shared 100% nucleotide sequence identity with the published sequence for CyHV-2. 相似文献
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2008年11月～2010年11月,采集山东海域大菱鲆、石鲽、鲈鱼各20批,按照世界动物卫生组织推荐的PCR检测方法对真鲷虹彩病毒病(Red Sea Bream Iridoviral Disease,RSIVD)进行初步调查.结果显示,共检出4例RSIVD感染样品.以真鲷虹彩病毒(Red Sea Bream Iridovirus,RSIV)和传染性脾肾坏死病毒(Infectious Spleen and Kidney Necrosis Virus,ISKNV)主要衣壳蛋白基因为基础,设计简并引物,PCR扩增本次检出阳性样品的RSIV/ISKNV MCP基因.将MCP基因PCR扩增产物测序,提交GenBank,并以MCP基因为基础,对被检出的阳性样品进行虹彩病毒属系统分类,绘制进化树.由进化树得出,4例阳性病毒株均属于虹彩病毒科细胞肿大病毒属. 相似文献
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The coat protein encoded by the nodavirus RNA2 gene originally isolated from greasy grouper, Epinephelus tauvina , was cloned, expressed as a recombinant polyhistidine-tailed fusion protein and characterized by immunoblot analysis. The purified recombinant protein was used to develop an indirect enzyme-linked immunosorbent assay (ELISA) to detect body exudate and plasma antibodies against the coat protein in both experimentally infected and commercial barramundi. In addition, the nucleotide sequence was employed to develop a RT–PCR detection assay based on the T4 region. The results showed that the virus could be detected as early as 3 days post-infection by RT–PCR while antibodies against the recombinant coat protein were detectable on day 6 post-infection. Among 112 commercial barramundi samples collected from October 1999 to April 2000, 9% showed positive ELISA results which were further verified by Western blot. 相似文献
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Abstract. Various possibilities of transmitting fish pathogenic VHS, SVC and IPN viruses by the heron, Ardea cinerea , were investigated. Shedding of IPN virus in the faeces could be demonstrated for a period of 7 days when about 1 g of IPN virus-infected trout fry was fed to the birds once only, whereas feeding larger quantities of infected fish on 5 consecutive days resulted in virus excretion for a further 5 days only. Infectious IPN virus could not be isolated from samples of blood and virus specific neutralizing antibodies could not be demonstrated in serum samples. A waterborne infection of trout fry was established by adding very small (0·2 g) quantities of faeces from herons fed IPN virus-contaminated fish to 20 1 water. Infectious SVC and VHS virus was also re-isolated from samples of food which were regurgitated by the herons at different times up to 120 min after feeding of contaminated fish. It was concluded that herons are able to act as mechanical vectors for IPN, VHS and SVC viruses and therefore, may be a potential source of infection and spread of the diseases. 相似文献