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1.
水稻第1染色体短臂粒长和粒宽QTL的精细定位 总被引：2，自引：0，他引：2
以第1染色体短臂RM1-RM3746和RM151-RM243区间内呈杂合、背景基本纯合的2个水稻剩余杂合体（RHL）衍生两个F6群体,将控制水稻粒长和粒宽的2个粒形QTL（qGL 1和qGW 1）定位于RM3746-RM243区间内。在此基础上,应用SSR标记检测,从其中1个群体中筛选到杂合区间分别为RM151-RM10404、RM10398-RM5359、RM10435-RM259和RM10381-RM243的4个单株,应用SSR标记进一步检测4套F2群体,从每套F2群体中分别筛选到母本珍汕97B和父本密阳46纯合型材料各10株,自交获得4套近等基因系材料并考查其粒长和粒宽。利用交迭重组染色体片段代换系分析法,将控制粒长和粒宽的QTL （qGL 1和qGW 1）界定于437.5 kb的RM10390-RM1344区间和392.9 kb的RM10376-RM10398区间,增效等位基因均来自母本珍汕97B,表明qGL 1和qGW 1是紧密连锁的不同座位。 相似文献
2.
在水稻品种南粳41中发现了一个黄绿叶自然突变体,经过多代连续自交形成了稳定的突变系,命名为ygl11(t),ygl11(t)整个生育期叶片都表现为黄绿色。对苗期、分蘖盛期、齐穗期突变体和野生型的叶绿素含量进行测定,ygl11(t)的叶绿素含量是野生型的45.7%~74.7%,叶绿素a含量是野生型的55.2%~87.5%,叶绿素b含量是野生型的12.5%~25.3%,ygl11(t)的类胡萝卜素的含量是野生型的62.3%~97.0%。ygl11(t)在分蘖盛期的净光合速率显著高于野生型,花后10d,ygl11(t)的净光合速率比野生型略低。对突变体叶片中叶绿体的超微结构进行观察,发现突变体叶绿体内的类囊体基粒片层数目减少且严重扭曲变形。遗传分析表明,ygl11(t)叶色性状受1对隐性核基因控制。利用SSR分子标记将YGL11(t)初步定位在水稻第10染色体的长臂上,进一步利用新开发的InDel和CAPS标记将YGL11(t)定位在58.1kb的物理距离内。对该区段内存在的开放阅读框进行序列分析,发现突变体ygl11(t)中编码叶绿素a氧化酶(chlorophyll a oxygenase 1)基因(OsCAO 1)的第9个外显子存在2个碱基缺失,从而导致提前出现终止密码子,初步分析OsCAO1即为YGL11(t)的候选基因。 相似文献
3.
粳稻发芽期耐碱性的QTL检测 总被引：2，自引：0，他引：2
以粳粳交高产106/长白9号的200个F2:3株系为作图群体,在0.15% Na2CO3溶液碱胁迫下,进行了水稻发芽率及其相对碱害率的鉴定评价,并以SSR标记构建的分子连锁图谱为基础,对水稻发芽率及其相对碱害率进行了数量性状基因座(QTL)检测。结果表明,在F3株系群中水稻发芽率及其相对碱害率均呈单峰接近正态的连续分布。共检测到碱胁迫下与水稻发芽率相关的QTL 7个,对表型变异的贡献率范围为4.05%～12.61%,其中位于第6染色体RM225-RM204区间的qGC 6和位于第9染色体RM219-RM3700区间的qGC 9对表型变异的贡献率分别为12.61%和10.85%。共检测到与水稻发芽率相对碱害率相关的QTL 6个,对表型变异的贡献率为4.82%～28.07%,其中位于第2染色体RM29-RM221区间的qRGC 2、位于第6染色体RM225-RM204区间的qRGC 6 1、位于第9染色体RM219-RM3700区间的qRGC 9和位于第12染色体RM260-RM3226区间的qRGC 12对表型变异的贡献率较大,分别为28.07％、15.35％、15.61％和18.91％,为主效QTL,但其相应的区间距离均较远,需要进一步深入研究。所检测的QTL增效等位基因主要表现为部分显性和超显性。 相似文献
4.
《杂交水稻》2010,(Z1)
GR38是自育的偏粳型广谱强恢复系种质,利用GR38分别与籼型不育系G46A和粳型不育系光A杂交的F2群体,应用集团分离分析方法和SSR标记对GR38的育性恢复基因进行分析。在组合G46A/GR38的F2群体中检测到2个独立的育性恢复QTL,分别位于第1染色体短臂上的SSR标记RM1331与RM3740之间和第1染色体长臂上的SSR标记RM5310与RM486之间;在组合光A/GR38的F2后代群体中只检测到1个育性恢复QTL,位于第1染色体短臂上的SSR标记RM3740与RM490之间。在两群体中均检测到的位于第1染色体短臂的育性恢复QTL,可能与已报道的Rf3等位,而位于第1染色体长臂上的恢复基因的可能为GR38上特有的新位点,命名为qRf(G-2)。 相似文献
5.
叶色突变是植物界广泛存在的一种现象,突变株系是解析植物光合作用和光形态建成等过程的理想材料。大麦黄绿叶色突变体ygl是本课题组在鄂大麦934的EMS突变体库中筛选获得的,该突变体的叶色在整个生育期都较野生型浅,而且苗期呈现黄绿色,后期表现为浅绿色。与野生型相比,ygl株高和千粒重分别表现为极显著和显著降低,其它重要产量性状,如穗数、穗长和穗粒数等无显著变化。进一步分析发现,ygl苗期叶片中基粒类囊体严重线性化,叶绿体超微结构受损。以正常叶色大麦品种Harrington和黄绿叶色突变体ygl为亲本构建了F_2分离群体,对F_2群体及其衍生的F_(2:3)家系进行分析发现,该性状由一个隐性核基因控制,命名为HvYGL(yellowgreenleaf)。利用上述F_2群体,通过基于SSR的BSA法,将该基因初步定位在大麦3H染色体上12.7 cM的区间内,其中 Bmag0209、 EBmac0871和 Bmag603三个标记与黄绿叶色性状共分离。 相似文献
6.
叶色突变体既可用于作物叶绿素合成、降解和光合作用等研究,也可作为标记基因为作物育种利用。本文对一个新发现的大豆黄绿叶自发突变体NJ9903-5进行遗传鉴定。结果表明:从对生真叶开始,该突变体幼嫩叶呈黄色,随着生长叶片逐渐转变为绿色。黄化叶片叶绿体数目下降,基质片层减少且排列疏松,叶绿素a、b、类胡萝卜素含量都极显著下降;其对株高、主茎节数、单株粒数、单株荚数有负效应,但对百粒重、蛋白质含量、油脂含量影响小,杂交后代中上述性状变异大。3个杂交群体遗传分析表明该性状受一对隐性核基因控制,利用F2隐性个体将目标基因ygl定位在SSR标记BARCSOYSSR_02_1445和BARCSOYSSR_02_1477之间约366 kb区段,包含36个候选基因。测序分析发现在突变体中,叶绿体膜转运蛋白相关基因Glyma.02G233700第1个外显子第38个碱基G缺失,移码突变导致蛋白翻译提前终止,结合前人研究结果,推测其为黄绿叶的目的基因ygl。 相似文献
7.
应用剩余杂合体衍生的近等基因系分解水稻产量性状QTL 总被引：5，自引：2，他引：3
以杂合区间为RM587-RM402的水稻剩余杂合体（RHL）衍生群体为材料,应用SSR标记检测,筛选到杂合区间分别为RM587-RM225、RM204-RM6119和RM6119-RM402的3个单株,进一步检测其F2群体,分别获得母本纯合型材料10株、父本纯合型材料10株和杂合型材料20株。种植这3套近等基因系材料,考查单株产量及其构成因子每株穗数、每穗实粒数和千粒重。经应用目标区间内等位基因效应分析和交迭重组染色体片段代换系分析,分解出3个控制每穗实粒数的QTL和2个控制单株产量的QTL,这些QTL分别位于物理距离为0.66 ~ 2.49 Mb的区间中,全部表现为加性作用为主,增效等位基因除qNFGP6 1来自父本密阳46外,其余均来自母本珍汕97B。提出了构建新型遗传材料,提高水稻QTL精细定位效率的策略。 相似文献
8.
《杂交水稻》2010,(Z1)
杂交水稻制种需对不育系割叶,此过程不但要求较高的操作技术和高强度的劳动,且对抽出的幼嫩稻穗也有伤害。本课题组在研究太湖流域粳稻地方品种资源生物多样性的过程中,发现了剑叶斜下伸的粳香糯A7444。本研究利用粳稻保持系863B(P1)与A7444(P2)进行配组,构建了P1、P2、F1、B1、B2和F2 6个世代,对剑叶角度进行分离分析。结果表明:剑叶角度受2对主基因+多基因控制,以主基因遗传为主。在分离分析的基础上,调查了P1与P2及其863B/A7444∥863B的B1世代141个单株SSR标记基因型和剑叶角度,构建该组合的SSR标记连锁图谱并分析剑叶角度QTL。利用WinQTLCart 2.5软件中的CIM方法检测到4个控制剑叶角度的QTL,其中位于第8染色体上的qFLA-8-1和qFLA-8-2为新的控制剑叶角度的主效QTL。qFLA-8-1位于SSR标记RM152-RM281之间,贡献率为55.24%,加性效应为65.33,增效等位基因来自863B,RM152在863B和A7444间扩增出的bp数分别为150 bp和145 bp,RM281在863B和A7444间扩增出的bp数分别为140 bp和145 bp。qFLA-8-2位于RM264-RM6215之间,贡献率为62.37%,加性效应为66.75,增效等位基因来自A7444,RM264在863B和A7444间扩增出的bp数分别为170 bp和167 bp,RM6215在863B和A7444间扩增出的bp数分别为185 bp和182 bp。 相似文献
9.
利用EMS诱变粳稻品种武运粳7号获得一个新窄叶突变体nal10,该突变体在苗期表现出极窄叶和弱小的特征,在生殖期表现出矮化、窄叶、畸颖和不育的表型。组织解剖学分析表明,nal10的叶片窄化可能是由于叶片大脉和小脉数量减少造成的。遗传分析表明,该窄叶表型受一对隐性核基因控制。利用nal10与台中本地一号(TN1)构建的F2群体,通过混合分离法,在第1染色体上找到3个紧密连锁的SSR标记(RM1287、RM562和RM5638)。进一步利用新发展的分子标记,最终将该基因定位在STS标记PK83和PK78之间的55.2 kb范围内,该区间共有8个开放阅读框。RT-PCR分析表明,NAL10的突变能够造成生长素生物合成、信号转导和运输基因转录水平的下降,因此NAL10是一个与生长素相关的窄叶新基因。这些结果为进一步克隆该基因、丰富水稻叶发育的遗传调控网络打下了基础。 相似文献
10.
太湖流域粳稻地方品种黑壳子粳抗稻瘟病基因的分子定位 总被引：4，自引：2，他引：2
以广谱、高抗稻瘟病的太湖流域粳稻地方品种黑壳子粳与感病品种苏御糯杂交,产生F1、F2、F2∶3及F5∶6重组自交系群体,用日本稻瘟病鉴别菌系北1接种鉴定。黑壳子粳对北1的抗性是由1对显性主效基因控制的,定名为Pi hk1(t) 。根据不同杂交世代群体对北1的抗、感反应,结合SSR分子标记,将黑壳子粳中的Pi hk1(t) 基因定位在水稻第11染色体长臂末端,与RM7654和RM27381两个标记的遗传距离分别为0.9 cM和1.6 cM。 相似文献
11.
A rice etiolation mutant 824ys featured with chlorophyll deficiency was identified from a normal green rice variety 824B.It showed whole green-yellow plant from the seedling stage,reduced number of tillers and longer growth duration.The contents of chlorophyll,chlorophyll a,chlorophyll b and net photosynthetic rate in leaves of the mutant obviously decreased,as well as the number of spikelets per panicle,seed setting rate and 1000-grain weight compared with its wild-type parent.Genetic analyses on F1 and F2 generetions of 824ys crossed with three normal green varieties showed that the chlorophyll-deficit mutant character was controlled by a pair of recessive nuclear gene.Genetic mapping of the mutant gene was conducted by using microsatellite markers and F2 mapping population of 495R/824ys,and the mutant gene of 824ys was mapped on the shon arm of rice chromosome 3.The genetic distances from the target gene to the markers RM218,RM282 and RM6959 were 25.6 cM,5.2 cM and 21.8 cM,respectively.It was considered to be a now chlorophyll-deficit mutant gene and tentatively named as chl11(t). 相似文献
12.
一个新的水稻叶绿素缺失突变基因的遗传分析和分子标记定位 总被引：4，自引：0，他引：4
从正常绿色水稻品种824B中发现1个黄化突变体824ys。该突变体具有叶绿素缺失突变特性,表现为植株黄绿色,分蘖数减少,生育期延长,总叶绿素、叶绿素a、叶绿素b的含量以及净光合速率比野生型亲本824B明显下降,每穗着粒数、结实率、千粒重等降低。对824ys与3个正常绿色品种杂交F1、F2的遗传分析表明,控制824ys的叶绿素缺失突变性状为1对隐性核基因。以495R/824ys F2作为定位群体,应用微卫星标记将824ys的叶绿素缺失突变基因定位于水稻第3染色体短臂,与RM218、RM282和RM6959等标记之间的遗传距离分别为25.6、 5.2和21.8 cM。认为该基因为一个新的水稻叶绿素缺失突变基因,暂命名为chl11(t)。 相似文献
13.
在自然光条件下水培籼稻品种Nankinkodo中,发现根为红色的自然突变体,命名为HG1。水培条件下该突变体在光照强度大于29 μmol/（m2·s）的可见光下,根开始转红,在光照强度为180 μmol/（m2·s）的可见光下,呈鲜红,具有明显的光照敏感性。遗传分析表明,该突变体光照敏感性的红根性状由1对显性基因控制, 暂命名为Lsr。利用微卫星标记将Lsr基因定位在第4染色体上RM252与RM303之间,遗传距离分别为9.8 cM和6.4 cM, 这为Lsr基因的精细定位和克隆奠定了基础。 相似文献
14.
LI Jin-bo XIA Ming-yuan WAN Bing-liang DU Xue-shu ZHA Zhong-ping YU Da-zhao QI Hua-xiong 《水稻科学》2009,16(1):79-82
A mutant with twisted hulls was found in a breeding population of rice (Oryza sativa L.). The mutant shows less grain weight and inferior grain quality in addition to twisted hulls. Genetic analysis indicated that the phenotype of mutant was controlled by a single recessive gene (temporarily designated as TWH). To map the TWH gene, an F2 population was generated by crossing the twh mutant to R725, an indica rice variety with normal hulls. For bulked segregant analysis, the bulk of mutant plants was prepared by mixing equal amount of plant tissue from 10 twisted-hull plants and the bulk of normal plants was obtained by pooling equal amount tissue of 10 normal-hull plants. Two hundred and seven pairs of simple sequence repeat (SSR) primers, which are distributed on 12 rice chromosomes, were used for polymorphism analysis of the parents and the two bulks. The TWH locus was initially mapped close to the SSR marker RM526 on chromosome 2. Therefore, further mapping was performed using 50 pairs of SSR primers around the marker RM526. The TWH was delimited between the SSR markers RM14128 and RM208 on the long arm of chromosome 2 at the genetic distances of 1.4 cM and 2.7 cM, respectively. These results provide the foundation for further fine mapping, cloning and functional analysis of the TWH gene. 相似文献
15.
16.
The light-sensitive red-root mutant, designated as HG1, was newly observed from an indica rice variety, Nankinkodo, when seedlings were grown with roots exposed to natural light. The root color of the mutant began to turn slight-red when the roots were exposed to the light at the intensity of 29 μmol/(m2·s), then turned dark-red at the light intensity of 180 μmol/(m2·s), suggesting that the root color of the mutant was evidently sensitive to light. Furthermore, genetic analysis showed that the character of ... 相似文献
17.
HUANG Ting-you WANG Zhi HU Yun-gao SHI Shou-pei PENG Tao CHU Xu-dong SHI Jun XIANG Zu-fen LIU Ding-you 《水稻科学》2008,15(2):153-156
To understand the genetic characteristics of a new photoperiod-sensitive genic male sterile line Mian 9S,some reciprocal crosses were made between Mian 9S and six indica rice materials,Yangdao 6,Luhui 602,Shuihui 527,Mianhui 725,Fuhui 838 and Yixiang 1B.Genetic analysis results suggested that the photoperiod-sensitive genic male sterility (PGMS) of Mian 9S was controlled by a single recessive nuclear gene.Thus,the F2 population derived from the cross of Yangdao 6/Mian 9S was used to map the PGMS gene in Mian 9S.By using SSR markers,the PGMS gene of Mian 9S was mapped on one side of the markers,RM6659 and RM1305,on rice chromosome 4,with the genetic distances of 3.0 cM and 3.5 cM,respectively.The gene was a novel PGMS gene and designated tentatively as pros4.In addition,the application of the pros4 gene was discussed. 相似文献
18.
An F2 population derived from the cross Zhong 9NR68 was used to map the fertility-restoring (Rf) gene for ID-type cytoplasmic male sterility (CMS).Two bulks (a fertile bulk and a sterile bulk) were constructed by pooling equal amount of ten highly fertile lines and ten highly sterile lines,respectively.Four hundred and thirteen pairs of simple sequence repeat (SSR) primers,which evenly distributed on 12 chromosomes of rice,were selected for analyzing polymorphisms between the parents and between the two bulks.The primer RM283 on chromosome 1 and the primers RM5756,RM258,RM6100 and RM171 on chromosome 10 were found to be polymorphic between the parents and between the two bulks.These five SSR markers were linked to fertility-restoring genes.A total of 82 excessive sterile lines were selected from Zhong 9NR68 F2 population to estimate the genetic distance between five SSR markers and fertility-restoring genes respectively.The results indicated that one Rf gene was linked to RM283 located on chromosome 1 at a distance of 6.7 cM,and the other Rfgene was mapped to the long arm of chromosome 10 flanked by RM258 and RM6100 at the distances of 8.0 cM and 2.4 cM,respectively. 相似文献
19.
FANG Li-kui SANG Xian-chun YANG Zheng-lin LIN Ying-hua WAN Nan HE Guang-hua 《水稻科学》2009,16(4):323-326
Tiller angle, a very essential agronomic trait, is significant in rice breeding, especially in plant type breeding. A tiller angle controlling 2 (tac2) mutant was obtained from a restorer line Jinhui 10 by ethyl methane sulphonate mutagenesis. The tac2 mutant displayed normal phenotype at the seedling stage and the tiller angle significantly increased at the tillering stage. A preliminary physiological research indicated that the mutant was sensitive to GA. Thus, it is speculated that TAC2 and TAC1 might control the tiller angle in the same way. Genetic analysis showed that the mutant trait was controlled by a major recessive gene and was located on chromosome 9 using SSR markers. The genetic distances between TAC2 and its nearest markers RM3320 and RM201 were 19.2 cM and 16.7 cM, respectively. 相似文献
20.
Seven residual heterozygous lines(RHLs)displaying different genotypic compositions in the genomic region covering probable locations of C (Chromogen for anthocyanin)gene on the short arm of rice chromosome 6 were selected from the progenies of the indica cross Zhenshan 97B/Milyang 46.Seeds were harvested from each of the seven plants,and the resultant F2:3 populations were used for fine mapping of C gene.It was shown in the populations that the apiculus coloration matched to basal leaf sheath coloration in each plant.By relating the coloration performances of the populations with the genotypic compositions of the RHLS,the C locus was located between rice SSR markers RM314 and RM253.By using a total of 1279 F2:3 individuals from two populations showing coloration segregation.the C locus was then located between RM111 and RM253,with genetic distances of 0.7 cM to RM111 and 0.4 cM to RM253.Twenty-two recombinants found in the two populations were assayed with seven more markers located between RM111 and RM253,including six SSR markers and one marker for the C gene candidate,OsC1.The C locus Was delimited to a 59.3-kb region in which OsC1 was located. 相似文献