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1.
To study the growth and yield of Acacia mangium in the Caribbean region of Colombia, allometric equations of total volume and aboveground plus coarse roots biomass were
fitted as a function of the tree’s diameter at breast height (dbh). The von Bertalanffy’s growth model and 59, 0.1 ha plots
(0.55–9.55 years old) were used to develop site index (SI) curves at 6 years base age. Then, using the state-space approach,
stand growth and yield models were developed for basal area, volume and biomass. The results show that A. mangium is a very promising species for timber production, atmospheric carbon removal and soil restoration because it grows very
fast even in mining degraded soils. On average sites it reaches 15 m in height in 3 years. However, early and reiterated thinning
coupled with initial mortality by cattle invasion of the very young understocked plantations are producing relatively low
yields. 相似文献
2.
A dramatic decline in forest cover in eastern Africa along with a growing population means that timber and poles for building
and fuelwood are in short supply. To overcome this shortage, the region is increasingly turning to eucalyptus. But eucalyptus
raises environmental concerns of its own. Fears that it will deplete water supply, affect wildlife and reduce associated crop
yields have caused many countries in the region to discourage farmers from planting this exotic. This paper is part of a series
of investigations on the growth and water use efficiency of faster growing eucalyptus hybrids, which was introduced from South
Africa to Kenya. The hypothesis is that the new hybrids are more efficient in using water and more suitable for the semi-arid
tropics than existing eucalyptus and two popular agroforestry species. Gas exchange characteristics of juvenile Eucalyptus grandis (W. Hill ex Maiden), two eucalyptus hybrids (E. grandis × Eucalyptus camaldulensis Dehnh.), Grevillea robusta (A. Cunn) and Cordia africana (Lam) was studied under field and pot conditions using an infrared gas analyzer was used to measure photosynthetic active
radiation (PAR), net photosynthetic rate (A), stomatal conductance (g
s) and transpiration rate (E) at CO2 concentrations of 360 μmol mol−1 and ambient humidity and temperature. A, E and g
s varied between species, being highest in eucalyptus hybrid GC 15 (24.6 μmol m−2 s−1) compared to eucalyptus hybrid GC 584 (21.0 μmol m−2 s−1), E. grandis (19.2 μmol m−2 s−1), C. africana (17.7 μmol m−2 s−1) and G. robusta (11.1 μmol m−2 s−1). C. africana exhibited high E values (7.0 mmol m−2 s−1) at optimal soil moisture contents than G. robusta (3.9 mmol m−2 s−1) and eucalyptus (5.3 mmol m−2 s−1) in field experiment and G. robusta (3.2 mmol m−2 s−1) and eucalyptus (4.2 mmol m−2 s−1) in pot-grown trees. At very low soil moisture content, extremely small g
s values were recorded in GC 15 and E. grandis (8 mmol m−2 s−1) and G. robusta (14 mmol m−2 s−1) compared to GC 584 (46.9 mmol m−2 s−1) and C. africana (90.0 mmol m−2 s−1) indicating strong stomatal control by the species. Instantaneous water use efficiency ranged between 3 and 5 μmol mmol−1 and generally decreased with decline in soil moisture in pot-grown trees but increased with declining soil moisture in field-grown
trees. 相似文献
3.
Characterization of strength properties of branchwood and stemwood of some tropical hardwood species
The mechanical strength properties of the branchwood of Aningeria robusta and Terminalia ivorensis with diameters ranging from 10 to 25 cm were examined to determine the suitability of branchwood as raw material for downstream processing. The study precisely assessed the static bending strengths, compression strengths and shear strengths parallel to the grain of the branchwood and stemwood of T. ivorensis and A. robusta. It was observed that under static bending, the overall (sapwood and heartwood combined) modulus of rupture and modulus of elasticity of the branchwood of A. robusta and T. ivorensis were lower than that of their corresponding stemwood. The results further show that the overall compression and shear strengths parallel to the grain of the branchwood of A. robusta and T. ivorensis were higher than that of their corresponding stemwood. Under static bending, compression parallel to the grain, and shear parallel to the grain, the experimental results indicate that the sapwood of the branchwood from both T. ivorensis and A. robusta had lower strength values than that of their corresponding heartwood. Similar results were recorded for the stemwood of T. ivorensis where the heartwood had higher strength values than the sapwood. However, in the case of the stemwood of A. robusta, the sapwood had higher strength values than the corresponding heartwood. 相似文献
4.
Carly Green Brian Tobin Michael O’Shea Edward P. Farrell Kenneth A. Byrne 《European Journal of Forest Research》2007,126(2):179-188
Reporting carbon (C) stocks in tree biomass (above- and belowground) to the United Nations Framework Convention on Climate
Change (UNFCCC) should be transparent and verifiable. The development of nationally specific data is considered ‘good practice’
to assist in meeting these reporting requirements. From this study, biomass functions were developed for estimating above-
and belowground C stock in a 19-year-old stand of Sitka spruce (Picea sitchensis (Bong) Carr.). Our estimates were then tested against current default values used for reporting in Ireland and literature
equations. Ten trees were destructively sampled to develop aboveground and tree component biomass equations. The roots were
excavated and a root:shoot (R) ratio developed to estimate belowground biomass. Application of the total aboveground biomass function yielded a C stock
estimate for the stand of 74 tonnes C ha−1, with an uncertainty of 7%. The R ratio was determined to be 0.23, with an uncertainty of 10%. The C stock estimate of the belowground biomass component was
then calculated to be 17 tonnes C ha−1, with an uncertainty of 12%. The equivalent C stock estimate from the biomass expansion factor (BEF) method, applying Ireland’s
currently reported default values for BEF (inclusive of belowground biomass), wood density and C concentration and methods
for estimating volume, was found to be 60 tonnes C ha−1, with an uncertainty of 26%. We found that volume tables, currently used for determining merchantable timber volume in Irish
forestry conditions, underestimated volume since they did not extend to the yield of the forest under investigation. Mean
stock values for belowground biomass compared well with that generated using published models. 相似文献
5.
Demand for goat (Capra hircus) meat in the southeastern USA is steadily increasing as a result of preferences exhibited by ethnic communities. Feeding
systems that include fodder trees can be developed to take advantage of the natural preference of goats for browse. Data were
collected for 2 years on a 5-year old stand of Robinia pseudoacacia L. to evaluate growth characteristics for goat browse and to develop allometric relationships in a randomized complete block
design (intra-row spacing 0.5 or 1 m and coppice height 0.25 or 0.50 m) replicated six times. Allometric equations were derived
from destructive harvests of 68 trees. Main branch size was not affected by intra-row spacing when trees were coppiced at
0.25 m; however, when coppiced at 0.5 m, trees spaced at 1 m had more and larger branches (P < 0.05) than trees spaced at 0.5 m. Intra-row spacing did not influence tree herbage biomass when trees were spaced at 0.5 m
whereas trees coppiced at 0.25 m and spaced at 1 m produced less woody and herbaceous biomass than trees spaced at 0.5 m (P < 0.05). A strong relationship (P < 0.0001) was found between tree herbage biomass and the number of main branches greater than 0.01 m (r
2 = 0.80). The results, based on growth characteristics and tree herbage biomass production, suggest that Robinia pseudoacacia L. would be an excellent candidate as a silvopastoral component in the southeastern USA. 相似文献
6.
To compare the responses to repeated flooding and drought of Salix gracilistyla, which grows on coarse gravel substrates, and Salix subfragilis, which grows on fine silt or clay substrates, we measured pre-dawn leaf water potential (Ψw
pd), osmotic adjustment (Ψw
tlp, Ψo
sat), and biomass production of cuttings under greenhouse conditions. The experimental design involved a control and four treatments
that crossed 1 or 3-week flooding (F) with 1 or 2-week droughts (D). Ψw
pd was reduced after 2 weeks of drought when preceded by 1 week of flooding. Neither species increased osmotic adjustment in
response to increased duration of drought between repeated 3-week flooding. Moreover, a decrease in the ratio of leaf biomass
to total biomass or an increase in the ratio of root biomass to total biomass with longer drought repetitions was not observed
for either species. The root ratio of S. gracilistyla was more strongly inhibited by flooding than that of S. subfragilis. The shoot-to-root ratio of S. subfragilis was higher than that of S. gracilistyla in all F combinations. The hypertrophied lenticel ratio of S.
gracilistyla after 1 week of flooding was nearly the same as that after 3 weeks of flooding, whereas values for S. subfragilis after 1 week of flooding were lower than those after 3 weeks of flooding. The low allocation to roots and the generation
of hypertrophied lenticels by S. gracilistyla in response to flooding, as compared with S. subfragilis, seem to be related to the different habitat substrate conditions of the two species. 相似文献
7.
In alley cropping systems, fast growing leguminous trees are pruned to reduce competition with crops for light and to provide
organic inputs for crop nutrition. Tree regrowth depends on non-structural carbohydrate reserves in the remaining tree parts.
In this study, the dynamics of starch and soluble carbohydrates in roots and stems of completely pruned (all shoots removed),
partially pruned (one branch retained on the pruned stump) and unpruned Erythrina poeppigiana (Walp.) O.F. Cook and Gliricidia sepium (Jacq.) Kunth ex Walp. trees were studied under humid tropical conditions in Turrialba, Costa Rica. Measurements on starch
and soluble carbohydrates in roots and stems were made at 0, 2, 6 and 12 weeks after pruning during both a “rainy” and a “dry”
season. In general, the dynamics of non-structural carbohydrates in roots and stems of pruned E. poeppigiana and G. sepium trees were similar. Starch concentration was highest in unpruned trees and higher in roots than in stems of pruned trees.
The effect of pruning intensity was first observed in stems, and starch reserves were more depleted in stems than in roots,
an effect more evident during the “dry” season. The critical tree regrowth stage for starch mobilisation was that of vigorous
sprout development at six or four weeks after pruning particularly in completely pruned trees. At this time, fine root biomass
and length and nodule biomass in pruned trees decreased. Survival of fine roots and nodules was greater in partially pruned
than in completely pruned trees. Starch accumulation in roots recommenced at 12 weeks after pruning in G. sepium, and later than 12 weeks after pruning in E. poeppigiana roots. This study showed that E. poeppigiana responded better to pruning regimes than G. sepium. Recovery of trees after pruning is better when trees are partially pruned than when completely pruned. 相似文献
8.
WANG Hui-mei ZU Yuan-gang 《林业研究》2007,18(4):316-318
UGPase gene related with wood cellulose synthesis was transferred into C. acuminata using the method of Agrobacte- rium-mediated genetic transformation, and an efficient transformation system was developed for C. acuminata on the basis of evaluations of several factors affecting Agrobacterium-mediated DNA transfer rate. The highest transformation rate was achieved when pre-cultttred leaf explants were infected with an Agrobacterium culture corresponding to OD600 (0.5) for 10 min, and cultured on explant regeneration medium for three days. The results of Southern hybridization showed that genomic DNA of the kanamycin-resistant shoots to an UGPase gene probe substantiated the integration of the transgene. Transformation efficiency (6%) was achieved under the optimized transformation procedure, This system should facilitate the introduction of important useful genes into C, acuminata. 相似文献
9.
Matthias Scheuber 《European Journal of Forest Research》2010,129(5):825-832
The k-nearest-neighbour (knn) method is known as a robust nonparametric method. It is used to estimate unknown values of data sets by means of similarity
to reference data sets with known values. The spectral information of satellite remote sensing data can be used to provide
the common characteristics in the knn estimation process. In forest sciences, the knn method is studied for its application potential. Some application examples are: (1) the estimation of parameters such as
basal area, stem volume, number of trees per diameter class and tree species; (2) the estimation of forest debris and non-wood
goods and services; (3) the production of wall-to-wall information for modelling, risk management and logistics. On the other
hand, different limitations with respect to methodological characteristics as well as the selection of suitable parameters
must be taken into consideration. The scope of this article concentrates on the discussion of the application potential and
limits of the knn method in forestry with particular emphasis on management planning needs. The study is based on data taken from a forest
inventory (FI) covering a test site near Rottenburg, in southwest Germany. Analysis results are compared with the traditional
outcome of inventory data analysis and partly presented in thematic maps, which show identical spatial distribution patterns.
For the map of six tree species, a map accuracy of 52.2% was found. The user’s accuracy for the prevailing tree species was
between 52.6% for Picea abies and 69.4% for Quercus sp. A timber volume map for Quercus sp. clearly visualises the bias at the extreme ends of the volume distribution. The root mean square error (RMSE) for the
total timber volume estimate was 30.9% for k = 5 and could be reduced to 22.6% for k = 20. For Quercus sp., however, the respective RMSE values were between 106.5 and 84.8%. Significant differences between FI and knn estimates
were mainly found for rare classes with minor representation in the reference data. 相似文献
10.
Goddert von Oheimb Christina Westphal Werner Härdtle 《European Journal of Forest Research》2007,126(3):359-370
The diversity, spatial patterns and temporal dynamics of dead wood were examined within the near-natural beech forests (Fagus sylvatica) of Serrahn (North-eastern Germany). Data were collected in an 8 ha sample plot and in two permanent plots (0.36 and 0.25 ha)
that had been established at the end of the 1960s. The mean volume of dead wood was 94 m3 ha−1, amounting to 14% of the total volume of all trees. The dead wood displayed a large variation in dead wood type, tree size
and decay class. Standing dead wood accounted for about one-third of the total dead wood volume. The densities of standing
dead trees were about 10% of the densities of the living trees over a wide range of diameters. The overall spatial distribution
of dead trees exhibited a random pattern. Among the different dead wood types, standing entire dead trees and uprooted trees
deviated from this pattern by displaying a significantly aggregated pattern. In the permanent plots a high mortality of overstorey
trees was observed (1.3% year−1) and the average amount of dead wood increased greatly from 2.9 to 111.6 m3 ha−1 over the 35-year observation period. The near-natural beech forests of Serrahn have experienced a long period of low human
interference. Nevertheless, our results suggest that the structure and dynamics of dead wood are strongly affected by the
last major disturbance events that took place at the end of the Middle Ages. Information about the forest history is therefore
a basic requirement when interpreting the results obtained in near-natural forests. 相似文献
11.
Bruno Esteves António Velez Marques Idalina Domingos Helena Pereira 《Wood Science and Technology》2007,41(3):193-207
Heat treatment of Pinus pinaster and Eucalyptus globulus woods, two important species in Portugal, was performed in the absence of air by steaming, inside an autoclave, for 2–12 h
at 190–210°C. Mass losses increased with treatment time and temperature reaching 7.3% for pine and 14.5% for eucalypt wood.
The wood behaviour with moisture was improved. The equilibrium moisture content decreased by 46% for pine and 61% for eucalypt,
the dimensional stability increased (maximum anti-shrinking efficiency in the radial direction of 57 and 90% for pine and
eucalypt, respectively) and the surface wettability was lowered. In relation to mechanical properties, the modulus of elasticity
was little affected (maximum decrease of 5% for pine and 15% for eucalypt) but the bending strength was reduced (by 40% at
8% mass loss for pine and 50% at 9% mass loss for eucalypt wood). The variation of properties was related to treatment intensity
and mass loss but significant improvements could already be obtained for a 3–4% mass loss without impairing the mechanical
resistance. The response of eucalypt was higher than that of pinewood. Heat treatment of eucalypt wood shows an interesting
potential to improve the wood quality for solid timber products. 相似文献
12.
Liriodendron are ideal plants for research on mating system, systemic evolution and population genetics. Here we report the development
and application of a set of simple sequence repeat (SSR) markers derived from expressed sequence tags (ESTs) of L. tulipifera. In total, 176 primer pairs were developed and yielded 132 EST-SSR markers that amplified clear SSR bands with genomic DNA
of L. tulipifera. Transferability of these EST-SSR markers to related species and genera was further examined. Twelve highly informative loci
screened from 132 EST-SSR markers were applied to explore the effective pollen dispersal patterns and male reproductive success
of Liriodendron. A provenance trial population of Liriodendron comprised 12 L. chinense (Hemsl.) Sarg provenances and 5 L. tulipifera provenances. The four maternal trees were randomly sampled as tested maternal trees in the population. Paternities of 220
open-pollination progenies from 4 maternal trees above were potentially identified by means of paternity analysis, of which
138 progenies (accounting for 62.7%) were assigned to 49 male parents at the 95% confidence level. The cumulative exclusion
probability of 12 EST-SSR loci was 98.5%. The average effective pollen dispersal distance of pollen donors ranged from 15
to 35 m, with the maximum dispersal distance of 77 m. There were obvious male reproductive success differences among individuals,
ranging from 0 to 10.9%. The cumulative male reproductive success of individuals within 35 m radius around the known maternal
trees reached 51.4%. Diversiform mating patterns, including selfing, intra-species mating and interspecies mating, coexisted
in the occasion of open-pollination in Liriodendron. 相似文献
13.
Melampsorella caryophyllacearum is a heteroecious fungus that produces disease on silver fir resulting in galls that depreciate the value of trees. Currently,
little is known about its epidemiology. The purpose of this study was to identify those site or management factors associated
with fir broom rust infection. A 29-plot systematic sampling was carried out throughout all silver fir forests in the Spanish
Pyrenees. In every sampling plot, we examined each tree for the presence of M. caryophyllacearum galls or witch’s brooms. We differentiated their position as either on the main stem or on the branches of trees. We identified
the most susceptible stands by relating a compendium of tree and stand variables with the presence and severity of symptoms.
Disease symptoms were observed in 11.8% (95% CI: 5.8–17.9%) of trees. Fir broom rust-infected trees tended to be those of
larger diameters. The rust infection was observed in 55.2% (95% CI: 35.7–73.6%) of the stands. We identified a wide range
of severities with 5 of 29 stands having more than 30% of trees infected. Fir broom rust appeared to be a widespread disease,
although we observed important differences in terms of severity. The presence and severity of the rust in stands was associated
with understories composed of more shade tolerant plant species and with longer periods without thinnings. Canopy opening
should be experimentally tested as a possible control method. 相似文献
14.
To determine the effect of shade on morphology, growth and biomass allocation in Picea sitchensis, Larix × eurolepis and Thuja plicata, seedlings were grown in the open or under shadehouses providing 25%, 50% and 75% reductions of full-light for two growing
seasons. For most of the characteristics assessed there was no significant interaction between species and shade indicating
that the morphological responses to changing shade treatments were not species-dependent. After two growing seasons the mean
height increment for the three species was significantly greater in 25% (76.1 cm) and 50% shade (74.9 cm) than in the open
(69.5 cm). Root collar diameter increment, shoot, root and total biomass declined significantly with increasing shade while
the opposite was true for the height:diameter ratio. In both western red cedar and hybrid larch the shoot:root ratio was significantly
greater in the shade while in Sitka spruce this characteristic was not influenced by shade. While all species had significantly
greater specific shoot areas in 75% shade than in 0% shade, this trend was particularly pronounced in hybrid larch. In hybrid
larch and western red cedar, the normalised specific projected shoot area increased significantly with increasing shade. The
opposite trend was observed for Sitka spruce. We conclude that in the main the species studied demonstrated similar shade
acclimation responses despite their reported differences in shade tolerance. 相似文献
15.
J. Roux B. Eisenberg A. Kanzler A. Nel V. Coetzee E. Kietzka M. J. Wingfield 《New Forests》2007,33(2):109-123
Plantations of Pinus spp. constitute approximately 50% of the South African forestry industry. The first aim of this study was to develop a reliable
inoculation technique to screen Pinus spp., for tolerance to infection by F. circinatum, which threatens pine forestry in South Africa. Inoculation of branches was compared with stem inoculations and we considered
the number of branches or trees required to obtain statistically significant results. Furthermore, variation in the susceptibility
of some Pinus families, clones and hybrids was considered. Results showed that branch inoculations were closely correlated with those from
stem inoculations, and that it is important to consider branch and stem diameters when assessing susceptibility of trees.
Subsequent trials using branch inoculations showed significant differences in F. circinatum tolerance amongst a range of pine species and hybrids of potential interest to forestry in South Africa. Significant differences
in susceptibility were also found among clones of two P. radiata families. The most tolerant trees were P. elliottii × caribaea and P. patula × oocarpa hybrids, while the most susceptible species were P. patula, P. greggii and hybrids of these two. This is the first trial considering the susceptibility of Pinus hybrids, Pinus clones and some P. patula provenances, and the results indicate excellent potential for breeding for tolerance to pitch canker in South Africa.
Application The accurate selection of disease tolerant planting stock for the South African forestry industry is crucially important
for the continued sustainability of this important industry. The work described here provides valuable information on an artificial
inoculation technique that will assist the industry in screening trees for tolerance to the pitch canker fungus, F. circinatum. It also provides some indication of the relative susceptibility of a number of Pinus spp., hybrids and families currently being evaluated in the country. 相似文献
16.
The effects of Timentin and cefotaxime (Cef) on shoot regeneration of the London plane tree (Platanus acerifolia Willd.) and their use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were compared. Shoot regeneration was significantly reduced on the media with Cef at concentrations from 100 to 500 mg·L–1. Timentin showed negative effect on plant regeneration at concentrations of 100 and 500 mg·L–1; however, 300 mg·L–1 Timentin was shown to facilitate shoot r... 相似文献
17.
Salinity is a major form of land degradation worldwide, with seasonal or temporary waterlogging often being an associated
issue. Acacia
ampliceps is a highly salt-tolerant small tree, naturally-occurring in north-western Australia, which produces abundant biomass with
potential for fuelwood and fodder. We investigated the extent of variation amongst provenances and families of A. ampliceps for seedling response to salt and waterlogging. Seven-week old seedlings from 27 families (seven provenances, grouped into
two regions), were treated in sand-filled pots in a glasshouse with either mixed salt (S) solutions (150 mol m−3 for 14 days, 300 mol m−3 for 33 days), waterlogging (W) or combined salt (S) and waterlogging (SW). Only 3% of seedlings died, mostly from the combined
salt × waterlogging treatment. Seedlings from the Kimberley region had significantly greater height and shoot biomass than
those from the Pilbara. Within these regions, Wave Hill (seedlot 14631) and Halls Creek (15738) provenances had the best growth.
Height growth was reduced similarly for S- and W-treated plants, whereas that of SW-treated plants was reduced much more.
Salt and waterlogging caused shoot dry weight reduction at harvest of 41 and 46%, respectively, with a slight interaction
between salt and waterlogging; SW treated plants were most affected. Generally there were relatively small differences in
the growth response of provenances and families to salt, but larger (often highly significant) variation in response to waterlogging.
Genotypic variation for shoot and phyllode water content and phyllode number in response to salt and waterlogging was generally
small. The good agreement found at the provenance level between seedling growth in this experiment and growth of young trees
on saline land in a trial in Pakistan suggests that glasshouse screening may be a useful means for selecting potential provenances
for field evaluation. 相似文献
18.
Root biomass and root distribution were studied in Entisols derived from the thick deposition of volcanic pumice on Hokkaido
Island, Japan, to examine the effect of soil conditions on tree root development. The soil had a thin (<10 cm) A horizon and
thick coarse pumiceous gravel layers with low levels of available nutrients and water. Two stands were studied: a Picea glehnii–Abies sachalinensis stand (PA stand) and a Larix kaempferi–Betula platyphylla var. japonica stand (LB stand). The allometric relationships between diameter at breast height (DBH) and aboveground and belowground biomass
of these species were obtained to estimate stand biomass. The belowground biomass was small: 30.6 Mg ha−1 for the PA stand and 24.3 Mg ha−1 for the LB stand. The trunk/root ratios of study stands were 4.8 for the PA stand and 4.3 for the LB stand, which were higher
than those from previous studies in boreal and temperate forests. All species developed shallow root systems, and fine roots
were spread densely in the shallow A horizon, suggesting that physical obstruction by the pumiceous layers and their low levels
of available water and nutrients restricted downward root elongation. The high trunk/root ratios of the trees may also have
resulted from the limited available rooting space in the study sites. 相似文献
19.
The efficiency of the botanical insecticide (BI) NeemAzal T/S (containing 1% Azadirachtin A), on the basis of azadirachtin
applied in a dose of 20 g a.i. ha−1 against Brassica pod midge (Dasineura brassicae), has been monitored for 4 years. The biological efficiency of BI was compared with the efficiency of some synthetic insecticides.
It was ascertained that BI was very efficient in decreasing the number of damaged oilseed rape pods (ranging from 56.5 to
85.9% compared to untreated plants) and its efficiency was comparable with synthetic insecticides based on Chloronicotinyl
(Thiacloprid) and Neonicotinoid (Acetamiprid). BI’s efficiency was, in some years, even significantly higher compared to pyrethroid
(λ-cyhalothrin). The high biological efficiency of azadirachtin that we ascertained was significantly reflected in increasing
potential crop yields. The yield increase of azadirachtin ranged between 9.3 and 19.4% compared to the control sample. Azadirachtin
showed the highest yield for the whole time of experimentation, and in some years the yield increase was significantly higher
compared to some synthetic agents. 相似文献
20.
MD. Atiar Rahman Takeshi Katayama Toshisada Suzuki Yuka Yoshihara Takashi Nakagawa 《Journal of Wood Science》2007,53(2):114-120
To clarify the biosynthetic pathway for syringyl lignans, especially syringyl tetrahydronaphthalene lignans and formation
of the C2–C7′ linkage, production of (+)-lyoniresinol (LYR) and its predicted intermediates [syringaresinol (SYR), 5,5′-dimethoxylariciresinol
(DMLR), and 5,5′-dimethoxysecoisolariciresinol (DMSLR)] in Lyonia ovalifolia var. elliptica was investigated by means of feeding experiments with radiolabeled precursors. Following individual administration of l-[U-14C]phenylalanine (Phe), [8-14C]sinapyl alcohol (SA), and [8,8′-14C]SYR to excised young shoots of L. ovalifolia and their subsequent metabolism, free [14C]lignans and [14C]lignan glycosides were extracted with methanol from stems and leaves and were divided into ethyl acetate-soluble fractions
(lignans) and aqueous fractions (lignan glycosides), respectively. Using a combination of xylanase, cellulase, and β-glucosidase, the glycosides were hydrolyzed to liberate [14C]lignans as aglycones. l-[U-14C]Phe was incorporated into (+)-[14C]SYR [stem 0.38%, 8% enantiomeric excess (e.e.)], (−)-[14C]SYR (leaves 2.75%, 72% e.e.), (+)-[14C]DMLR (stem 0.07%, 18% e.e. and leaves 0.009%, 58% e.e.), (−)-[14C]DMSLR (stem 0.03%, 46% e.e. and leaves 0.05%, 20% e.e.), (+)-[14C]LYR (leaves 0.013%, 22% e.e.) and glycosides of (+)-[14C]LYR (stem 0.036%, 50% e.e.) in 24h. Based on the percent incorporation and enantiomeric composition of the lignans, the
biosynthetic pathway of (8R,8′R)-(+)-LYR was proposed as follows: a nonselective dehydrogenative dimerization of sinapyl alcohol yields (±)-SYR, which is
reduced with low specificity to give (8R,8′R)-(+)-DMLR. This is cyclized to directly give (+)-LYR as well as reduced again to (8R,8′R)-(−)-DMSLR. Although further transformation of (−)-DMSLR also leads to the formation of (+)-LYR, cyclization could be a main
pathway for (+)-LYR biosynthesis.
This report was presented at the IAWPS 2005 International Symposium on Wood Science and Technology, Yokohama, November 2005 相似文献