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1.
将WGA-HRP注入75~90日龄鸡肾上腺内,追踪支配鸡肾上腺传出神经元的胞体。结果显示,鸡肾上腺接受交感性节前和节后神经元的支配,以节后神经元支配占主导;迷走神经的传出神经元也支配鸡肾上腺,但处于次要地位  相似文献   

2.
鸡肾上腺神经的分布——WGA—HRP法研究   总被引:2,自引:0,他引:2  
将WGA-HRP注入75 ̄90日龄鸡肾上腺内,追踪支配鸡肾上腺传出神经元的胞体,结果显示:鸡肾上有朱接受交感神经节前和节后神经元的支配,以节后神经元支配占主导;迷走神经的传出神经元也支配鸡肾上腺,并处于次要地位。  相似文献   

3.
鸡盲肠传出神经元分布的研究   总被引:1,自引:1,他引:1  
将100~150mL/L HRP水溶液注入成年母鸡左侧盲肠的浆膜下,研究鸡盲肠传出神经元的分布。结果表明:支配盲肠的交感节后神经元位于T3-LS1交感干神经节(占64.7%),峰值位于T5-T6交感干神经节,少数位于肾上腺神经节(占17.8%)和内脏神经节(占17.5%);在肠神经内有大量标记细胞;在延脑迷走神经核中未出现标记的神经元;在盆神经内未看到被标记的支配盲肠的副交感节后神经元。  相似文献   

4.
为了找出支配鸡胆囊交感节后神经元的分布规律,选用体重1.5 kg~2.5 kg的成年母鸡6只,将CT-HRP溶液注入胆囊壁,动物存活3 d~4 d后,经左心室灌流固定,取内脏神经节、肾上腺神经节以及双侧胸、腰和荐段交感干神经节,制成50 μm的连续冰冻切片,TMB法呈色反应,置明视野显微镜下观片统计.结果发现,支配鸡胆囊的交感传出神经元胞体位于内脏神经节(占41.1%)、肾上腺神经节(占40.5%)和T2~T7交感干神经节(占18.4%),在交感干神经节中标记细胞的峰值位于T5、T6交感干神经节.所有的标记细胞以位于右侧的占优势.  相似文献   

5.
母鸡输卵管子宫部副交感节后神经元支配的逆行追踪研究   总被引:6,自引:2,他引:4  
将CB-HRP溶液注入鸡输卵管子宫部浆膜下,取泄殖腔神经节和双侧阻部内神经以及肠神经直肠段,制成50μm厚度的边疆冰冻切片,TMB反应,明视野观察。结果发现:鸡输卵管子宫部受泄殖腔神经节和双侧阻部内神经节的副交感节后神经元支配;双侧阴部内神经节的标记细胞无显著差异;说明尽管母鸡输卵管子宫部是单侧发育器官,但其受双侧阴部内神经的融交感节后神经元的支配。鸡输卵管子宫部还受肠神经的节后神经元的支配。另外本实验对汇殖腔神经节、阴部内神经及肠神经直肠段进行了大体解剖观察。  相似文献   

6.
用霍乱毒素结合辣根过氧化物酶逆行追踪法对鸡胰腺的交感传出神经元的定位进行了研究.结果表明,鸡胰腺的交感传出神经元位于双侧的肾上腺神经节、内脏神经节和T3-T7交感干神经节,在交感干神经节中集中分布节段在T5交感干神经节.  相似文献   

7.
为了解支配鸡肝的交感传出神经元的分布规律,将CT—HRP溶液注入鸡肝门周围区,动物存活3~4d后,经左心室灌流固定,取内脏神经节、肾上腺神经节及双侧胸、腰和荐段交感干神经节,制成50μm厚的连续冰冻切片,TMB法呈色反应,明视野显微镜下观察统计。结果发现,支配鸡肝的交感传出神经元胞体位于内脏神经节(占41.9%)、肾上腺神经节(占41.7%)和T2~T7交感干神经节(占16.4%)。在交感干神经节中,标记细胞的峰值位于T5、T6交感干神经节。  相似文献   

8.
将CB-HRP溶液注入环颈雉泄殖腔的一侧壁内,逆行追踪其传出神经元,实验结果表明:①标记的节后神经元出现于双侧S4-S9交感干神经节内,左右侧泄殖腔神经节内,以注射侧为主。②标记的节前神经元分别出现于T6-L3髓节和S3-S8髓节内,但以同侧为主。  相似文献   

9.
肠神经对鸡输卵管支配的逆行追踪法研究   总被引:7,自引:1,他引:6  
应用HRP和CB—HRP逆行追踪支配鸡输卵管神经元在肠神经内的分布和形态特点,以探察鸡肠神经在体内的支配范围以及肠神经时生殖系统的可能调节作用。结果显示,肠神经内出现大量阳性神经元和神经纤维,这些神经元大多数为小型多极神经元,少数是双极神经元。阳性细胞主要位于肠神经节内,节间束也见个别分布。这就从形态上证实,肠神经支配并调节鸡的生殖活动。本研究还比较了HRP和CB—HRP2种示踪物在标记神经元时的敏感性和反应强度。  相似文献   

10.
应用SABC法免疫组织化学技术,探索鸡肠神经内胆碱乙酰转换酶(ChAc)和多巴胺羟化酶(DβH)神经元的形态及其分布。结果显示,肠神经内含有52%的ChAc神经元和36%的DβH神经元,2种神经元胞体或为多突起的多边形,或为一端有长突起的椭圆形,它们分布于神经内的神经纤维之间。DβH神经元在节间束也有个别分布。部分神经纤维呈ChAc或DβH阳性。这表明鸡肠神经系混合神经,既含副交感节后神经元,也含交感节后神经元。  相似文献   

11.
Sympathetic, parasympathetic and sensory neurons were labeled by injections of horseradish peroxidase into various regions of the heart in 33 Beijing ducks. Sympathetic postganglionic neurons innervating the heart were located in the paravertebral ganglia C15 (C16 is the last cervical segment in the duck) to T3, especially in the ganglion T1. The coronary sulcus and ventricle were more abundantly innervated by sympathetic neurons than the atrium. The left side of the heart was preferentially innervated by sympathetic postganglionic neurons in the left side of paravertebral ganglia but the right side of the heart were equally supplied from the right and left ganglia. Within the medulla oblongata, the number of labeled vagal preganglionic neurons in the nucleus ambiguus was much greater than that in the dorsal motor nucleus of the vagus nerve. Labeled neurons of the nucleus ambiguus were found in many ducks injected into the coronary sulcus. Cardiac sensory neurons were observed in the dorsal root ganglia C15 to T2 (highest in the ganglion T1) and in the nodose and jugular ganglia of the vagus nerve. These labeled neurons probably form the afferent and efferent limbs of cardiac reflexes and control circulation in the Beijing duck.  相似文献   

12.
Sympathetic postganglionic and sensory neurons were labelled by injections of horseradish peroxidase into the testis of the male chicken. The total number of labelled neurons in the paravertebral, prevertebral, dorsal root and nodose ganglia was 943 on average for five chickens. Sympathetic postganglionic neurons were located in the paravertebral ganglia T3-LS3 (10% of the total number of labelled neurons), especially in T6 and T7, and in the prevertebral ganglia adjacent to the adrenal glands and aorta (19%). They were found almost ipsilaterally. No labelled neurons were observed in the dorsal motor nucleus of the vagus. Sensory neurons were found bilaterally in the dorsal root ganglia T2-LS3 (71%), especially in T5 to T7. Over a quarter of labelled sensory neurons were located in the contralateral dorsal root ganglia. In the nodose ganglia, only a few labelled sensory neurons were observed (much less than 1%). These results indicate that, unlike the ovary, the testis of the chicken tends to be innervated by ipsilaterally located sympathetic postganglionic and sensory neurons, with the sensory neurons being more numerous than the sympathetic postganglionic neurons.  相似文献   

13.
应用CB-HRP溶液注入母鸡输卵管子宫部浆膜下,四甲基联苯胺(TMB)组织化学呈色,以探察母鸡输卵管子宫部初级感觉神经元的定位。结果表明:母鸡输卵管子宫部的初级感觉神经元位于双侧T1-LS13脊神经节、颈静脉神经节和结状神经节。标记细胞数分布不均,在体左侧多于体右侧,在脊神经节多于颈静脉神经节和结状神经节。在脊神经节内标记细胞有T5-LS1和LS8-LS11前后两个相对集中区,峰值分别在T7和LS11。说明尽管母鸡输卵管子宫部是单侧发育成熟脏器,但其感觉沿双侧脊神经和迷走神经传人中枢,以体左侧传入为主;且感觉经脊神经传入较迷走神经传入中枢优势明显。  相似文献   

14.
艇HRP法对兔减压神经进行了研究,标记的传入神经元绝大多数位于迷走神经结状节尾侧半部偏一侧,少数见于颈静脉神经节,其中枢突标记终末见于延髓闩以上的孤束核内侧部,腹侧部以及迷背核内。在颈前节也出现标记细胞,表明减压神经中也含有起源于 前节的交感节后纤维成分。主动脉压力感受器有经减压神经来的感觉神经交感节后神经纤维支配。提示减压神经是含有一般内脏传入和一般内脏传出纤维的混合神经,为研究心血管生理提供了  相似文献   

15.
The purpose of this investigation was to determine the central distribution of the efferent neurons of the recurrent laryngeal nerve (RLN) in the sheep by the use of the retrograde transport of the fluorescent tracer Fast Blue. The distribution of the RLN neurons was also compared with that of the neurons simultaneously labelled by injection of another tracer, Diamidino Yellow dihydrochloride, into the cervical trunk of the vagus nerve (CTV). Injections of the tracer into the CTV resulted in heavy retrograde labelling of neurons in the ipsilateral dorsal motor nucleus of the vagus nerve, in the nucleus ambiguus, in the nucleus retroambigualis and in the reticular formation surrounding the nucleus ambiguus. Following injections of the tracer into the RLN, labelling of neurons was seen over a wide area of the ipsilateral nucleus ambiguus and in the nucleus retroambigualis. Species differences in the distribution of the efferent component of the RLN are discussed, in particular ruminants compared to nonruminants.  相似文献   

16.
对青海省大通县新生牦牛犊肾上腺的形态结构进行了观测。结果:右肾上腺呈微扁不规则的锥体形或靴形。左肾上腺呈外侧略突,腹侧中部略凹的豆形。肾上腺的总重量占体重的0.014%,左肾上腺略大于右肾上腺。  相似文献   

17.
The distribution of sympathetic and sensory neurones innervating the submandibular gland (SMG) in sheep was studied using retrograde tracing technique. The retrograde tracer Fast Blue (FB) was unilaterally injected into the SMG in five juvenile male sheep under general anaesthesia. After a 4-week survival period, all the animals were reanaesthetized, perfused transcardially with 4% buffered paraformaldehyde and ganglia, which could be considered as a potential sources of sympathetic, and afferent innervation of the gland were bilaterally collected. The FB-labelled sympathetic neurones were found in the ipsilateral superior and middle cervical ganglion. Many labelled neurones were distributed in the ipsilateral jugular and nodose ganglia of the vagus nerve and smaller numbers of the nerve cells were also found in ipsilateral C1-C3 dorsal root ganglia (DRG). No labelled neurones were observed in the ipsilateral stellate ganglion, trigeminal ganglion, C4-C8 DRG and in all contralateral ganglia. The present study revealed that the majority of sympathetic neurones projecting to the sheep SMG are found in the superior cervical ganglion but some of them are also distributed in the middle cervical ganglion. Most of the afferent neurones are located in the jugular and nodose ganglia of vagus nerve but C1-C3 DRG also comprise some of these nerve cells.  相似文献   

18.
The distribution of neurons projecting to the mammary gland was investigated by using the retrograde tracing method in juvenile pigs (n = 12). Fluorescent retrograde tracer Fast Blue (FB) was injected into the nipple (n = 3) or parenchyma (n = 3) of the second, right thoracic mamma or into the nipple (n = 3) and parenchyma (n = 3) of the last, right abdominal mamma. FB-positive (FB+) mammary gland-projecting neurons were found in some right dorsal root ganglia (DRG) and sympathetic chain ganglia (SChG) only. After injection of the tracer into the second, right thoracic mamma, FB+ neurons were observed in Th9-Th12 DRG but most of them were located in Th11 and Th12 ganglia. As concerns SChG, FB+ neurons were found in Th1-Th4, Th7-Th14 and L1-L4 ganglia. The vast majority of them were located in Th10 and Th11 SChG, which appeared to be the main sources of efferent innervation of this mamma. Neurons projecting to the last right abdominal mamma were found in L1-L3 DRG and L1-L4 SChG but most of them were located in L1-L2 ganglia and L1-L2 ganglia, respectively. This study for the first time has disclosed the localization of neurons supplying the mammary gland in larger domestic animal species, the pig, by using the retrograde tracing method.  相似文献   

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