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1.
Legume–cereal intercropping is increasingly being appreciated in dryland areas, where severe climatic conditions and intensive agricultural practices, generally dominated by continuous cereal cultivation, determine depletion of soil nutrient resources and decline of soil fertility. This research aimed to assess whether and to what extent a newly introduced legume-based intercropping system is able to ameliorate the biological fertility status of an arable soil in a way that is still noticeable during the succeeding durum wheat cropping season in terms of changes in bacterial community structure, soil C and N pools, and crop yield. A field experiment was carried out under rainfed conditions in Southern Italy on a sandy clay loam soil cultivated with durum wheat following in the rotation a recently established grain legume (pea, faba bean)–barley intercropping. Soil chemical, biochemical and eco-physiological variables together with compositional shifts in the bacterial community structure by LH-PCR fingerprinting were determined at four sampling times during the durum wheat cropping season. Soil fertility was estimated by using a revised version of the biological fertility index. Results showed that even though the microbial biomass was significantly altered, the preceding legume intercrops stimulated C-related functional variables thus leading to an increased release of mineral N, which was larger in crop treatments succeeding pea-based than faba bean-based intercropping. The increased N made available in soil enabled the succeeding durum wheat to achieve an adequate grain yield with a reduced N-fertilizer use. Soil type and environmental conditions rather than crop treatments were major determinants of bacterial community structure. The biological fertility status was not varied, suggesting that in intensively managed rainfed areas long-term crop rotations with intercropped legumes are needed to consistently ameliorate it.  相似文献   

2.
Maintaining soil organic carbon (SOC) in arid ecosystem is important for soil productivity and restoration of deserted sandy soil in western plain of India. There is a need to understand how the cropping systems changes may alter SOC pools including total organic carbon (TOC), particulate organic C (POC), water soluble carbon (WSC), very labile C (VLC), labile C (LC), less labile C (LLC) and non-labile C (NLC) in arid climate. We selected seven major agricultural systems for this study viz., barren, fallow, barley–fallow, mustard–moth bean, chickpea–groundnut, wheat–green gram and wheat–pearl millet. Result revealed that conversion of sandy barren lands to agricultural systems significantly increased available nutrients and SOC pools. Among all studied cropping systems, the highest values of TOC (6.12 g kg?1), POC (1.53 g kg?1) and WSC (0.19 g kg?1) were maintained in pearl millet–wheat system, while the lowest values of carbon pools observed in fallow and barren land. Strong relationships (P < 0.05) were exhibited between VLC and LC with available nutrients. The highest carbon management index (299) indicates that wheat–pearl millet system has greater soil quality for enhancing crop productivity, nutrient availability and carbon sequestration of arid soil.  相似文献   

3.
A long‐term experiment comparing no‐till with conventional tillage systems across five rotations was evaluated 11 years after initiation. The objectives of the present paper are (1) to report differences in soil chemical properties (namely soil organic matter, total nitrogen, phosphorus, potassium and pH) that have resulted by converting from conventional to no‐till under contrasting cropping systems and (2) to draw tentative conclusions and recommendations on fertility status and fertilizer use and management. Soil in the no‐till system had increased surface soil organic C levels relative to conventional tillage regardless of rotation. In addition, depending on the rotation, the N and P content of the soil improved with no‐till compared with conventional tillage. In other words, no‐till has helped to retain soil organic matter (SOM), conserved more N, and resulted in increased extractable P and exchangeable K concentrations in the upper root‐zone. Hence, wheat produced in a no‐till system may receive more nutrients from decomposition of SOM and acidification of the seed zone. It is possible that lesser amounts of fertilizer nutrients will be needed because of the greater efficiency of nutrient cycling in no‐till systems relative to conventional systems. Copyright © 2001 John Wiley & Sons, Ltd.  相似文献   

4.
Soil organic matter (SOM) and its different pools have key importance in nutrient availability, soil structure, in the flux of trace gases between land surface and the atmosphere, and thus improving soil health. This is particularly critical for tropical soils. The rates of accumulation and decomposition of carbon in SOM are influenced by several factors that are best embodied by simulation models. However, little is known about the performance of SOM simulation model in an acid tropical soil under different tillage systems including no-tillage (NT). Our objective was to simulate soil organic matter dynamics on an Acrisol under no-tillage and different plowed systems using Century model. Tillage systems consisted of no-tillage, disc plow, heavy disc harrow followed by disc plow, and heavy disc harrow. Soil C stocks simulated by Century model showed tendency to recovery only under no-tillage. Also, simulated amounts of C stocks of slow and active pools were more sensitive to management impacts than total organic C. The values estimated by Century of soil C stocks and organic carbon in the slow and passive pools fitted satisfactorily with the measured data. Thus fitted, except for the active pool, Century showed acceptable performance in the prediction of SOM dynamics in an acid tropical soil.  相似文献   

5.
The crop rotation system in organic farming is a determinant factor to accumulate and preserve soil organic matter (SOM), and in depth knowledge on its effects is still lacking. Tillage intensity in particular is crucial to maintain soil aggregates and protect SOM from degradation. The evolution of SOM was tested in two adjacent fields under two different rotation cropping systems (low-intensity tillage and high-intensity tillage), and the effect of a further cultivation of legume in both fields was evaluated using 13carbon (C)-nuclear magnetic resonance (NMR) and elemental analysis of samples isolated through combined aggregate size and density fractionation. The two adjacent fields had been managed using the following organic farming methods for 13 seasons since 1998: i) alfalfa-based, with nitrogen (N) enrichment and low-frequency tillage with alfalfa (Medicago sativa) (9 seasons), winter wheat (Triticum durum) (3 seasons), and broad bean (Vicia faba) (1 season) and ii) cereal-based, with N depletion and annual tillage with barley (Hordeum vulgare) (7 seasons), sunflower (Helianthus annuus) (2 seasons), broad bean (Vicia faba) (3 seasons), and bare fallow (1 season). Soil sampling was carried out at the end of the 13-year rotation (T0, November 2011) and after winter wheat and chickpea cultivation in both fields over two subsequent years (T1, July 2013). Bulk organic C was significantly higher in the alfalfa-based system than in the cereal-based system at both T0 and T1, with SOM occluded in soil aggregates and associated with mineral particles. In terms of the macroaggregates heavy fraction at T0, the alfalfa-based field contained twice the organic C of that in the cereal-based field, as well as three times the organic C in the occluded particulate organic matter (POM). The occluded POM (oPOM) had a lower aryl/O-alkyl C ratio in the alfalfa-based system than in the cereal-based system, suggesting that oPOM undergoes a lower degree of decomposition during low-intensity management. The aryl/O-alkyl C ratios of the macro-and microaggregate oPOM decreased from T0 to T1 in the cereal-based system, suggesting increased protection of these fractions by soil aggregates. Thus, including legumes in crop rotation appears to positively affect the accumulation of SOM associated with mineral particles and within soil aggregates.  相似文献   

6.
Soil organic matter (SOM) has long been recognized as an important indicator of soil productivity. The SOM refers to the organic fraction of the soil exclusive of undecayed plant and animal residues. It plays a crucial role in maintaining sustainability of cropping systems by improving soil physical (texture, structure, bulk density, and water-holding capacity), chemical (nutrient availability, cation exchange capacity, reduced aluminum toxicity, and allelopathy), and biological (nitrogen mineralization bacteria, dinitrogen fixation, mycorrhizae fungi, and microbial biomass) properties. The preservation of SOM is crucial to ensure long-term sustainability of agricultural ecosystems. Improvement/preservation of soil organic matter can be achieved by adopting appropriate soil and crop management practices. These practices include conservation tillage, crop rotation, use of organic manures, increasing cropping intensity, use of adequate rate of chemical fertilizers, incorporation of crop residues, liming acidic soils, and keeping land under pasture. Organic matter can adsorb heavy metals in the soils, which reduce toxicity of these metals to plants and reduce their escape to ground water. Similarly, SOM also adsorbs herbicides, which may inhibit contamination of surface and ground water. Furthermore, SOM also functions as a sink to organic carbon and mitigates carbon dioxide (CO2) gas escape to the environment. Globally, soil organic matter contains about three times as much carbon as found in the world's vegetation. Hence, organic matter plays a critical role in the global carbon balance that is thought to be the major factor affecting global warming. Overall, adequate amounts of soil organic matter maintain soil quality, preserve sustainability of cropping systems, and reduce environmental pollution.  相似文献   

7.
Management practices including various tillage systems influence quantity and composition of soil organic matter (SOM). Parameters for evaluating both the SOM quantity (organic C [Cox], total N [Nt]) and quality (microbial biomass C, hydrophobic and hydrophilic organic components) were determined in soil samples, taken from two soil depths (0–0.1 m and 0.1–0.3 m) in a field experiment in the period 2001–2007, with different tillage systems. The experiment, founded in 1995 in Prague-Ruzyně, includes conventional soil tillage (CT) plus some selected methods of conservation tillage: (a) no tillage (NT), (b) no tillage + mulch (NTM), and (c) minimum tillage with pre-crop residues incorporated (MTS). Cox and microbial biomass C contents increased significantly with conservation tillage as compared to CT in 0–0.1 m layer, non-significant increase was found in 0.1–0.3 m layer. Nt increased non-significantly in both layers. Along with the depth of sampling, the content of the characterized parameters decreased in all variants; but the decrease in the conventionally tilled variant was, for the most part, lower than in the conservation tillage. The functional hydrophobic and hydrophilic groups of soil organic matter were identified by Fourier transform infrared (FTIR) spectroscopy, and the hydrophobic/hydrophilic group intensities ratio was calculated as the parameter of soil hydrophobicity. A higher soil hydrophobicity existed in all three conservation tillage treatments compared to CT due to the significantly higher content of hydrophobic organic components. Cox correlated significantly with microbial biomass C, Nt, hydrophobic components, and soil hydrophobicity (R = 0.552–0.654; P < 0.05). Hydrophilic components did not correlate with other soil characteristics, with the exception of hydrophobic components. These data show that shifting from CT to the conservation tillage systems increased the content of SOM in top soil layer in relatively short time, improved the SOM quality and increased soil hydrophobicity in the condition of experiment.  相似文献   

8.
Quality of agricultural soils is largely a function of soil organic matter. Tillage and crop management impact soil organic matter dynamics by modification of the soil environment and quantity and quality of C input. We investigated changes in pools and fluxes of soil organic C (SOC) during the ninth and tenth year of cropping with various intensities under conventional disk-and-bed tillage (CT) and no tillage (NT). Soil organic C to a depth of 0.2 m increased with cropping intensity as a result of greater C input and was 10% to 30% greater under NT than under CT. Sequestration of crop-derived C input into SOC was 22±2% under NT and 9±4% under CT (mean of cropping intensities ± standard deviation of cropping systems). Greater sequestration of SOC under NT was due to a lower rate of in situ soil CO2 evolution than under CT (0.22±0.03 vs. 0.27±0.06 g CO2–C g−1 SOC yr−1). Despite a similar labile pool of SOC under NT than under CT (1.1±0.1 vs. 1.0±0.1 g mineralizable C kg−1 SOC d−1), the ratio of in situ to potential CO2 evolution was less under NT (0.56±0.03) than under CT (0.73±0.08), suggesting strong environmental controls on SOC turnover, such as temperature, moisture, and residue placement. Both increased C sequestration and a greater labile SOC pool were achieved in this low-SOC soil using NT and high-intensity cropping.  相似文献   

9.
The productivity of agricultural areas in semi‐arid regions can be improved through the use of irrigation. However, the intensive cropping of such soils can have detrimental effects, especially with regard to soil organic matter (SOM) pools. The goal of this work was to evaluate soil organic carbon and nitrogen stocks of different irrigated agricultural systems and compare these to preserved natural ecosystems adjacent to each of the cropping systems. We selected four cropping systems: banana, a maize/bean succession (MB), pasture (P) and guava (G), as well as areas covered by native vegetation. Stocks of total soil organic carbon (TOC), amounts of unprotected and protected soil organic carbon, carbon and nitrogen in microbial biomass and microbial respiration were quantified. Surface soil TOC stocks under banana, G and P grass were significantly greater than under native vegetation and MB system. The most intensive management system was the MB, and the least intensive systems were P and G. The least intensive cropping systems were grouped on the basis of similarities in TOC, POC, total soil nitrogen and N in microbial biomass stocks. These results show that the degree of soil degradation resulting from changes in land use systems increases with the intensity of the land use systems themselves. This confirms the established hypothesis that the extent of degradation of soil properties and changes in some SOM fractions depend on the intensity of soil use. Furthermore, the adoption of conservation practices may remediate soil degradation and increase SOM stocks, mainly at the soil's surface. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   

10.
In this study, we investigated crop yield and various chemical and microbiological properties in rhizosphere of wheat, maize, and faba bean grown in the field solely and intercropped (wheat/faba bean, wheat/maize, and maize/faba bean) in the second and third year after establishment of the cropping systems. In both years, intercropping increased crop yield, changed N and P availability, and affected the microbiological properties in rhizosphere of the three species compared to sole cropping. Generally, intercropping increased microbial biomass C, N, and P availability, whereas it reduced microbial biomass N in rhizosphere of wheat. The rhizosphere bacterial community composition was studied by denaturing gradient gel electrophoresis of 16S rRNA. In the third year of different cropping systems, intercropping significantly changed bacterial community composition in rhizosphere compared with sole cropping, and the effects were most pronounced in the wheat/faba bean intercropping system. The effects were less pronounced in the second year. The results show that intercropping has significant effects on microbiological and chemical properties in the rhizosphere, which may contribute to the yield enhancement by intercropping.  相似文献   

11.
Quantifying seasonal dynamics of active soil C and N pools is important for understanding how production systems can be better managed to sustain long-term soil productivity especially in warm subhumid climates. Our objectives were to determine seasonal dynamics of inorganic soil N, potential C and N mineralization, soil microbial biomass C (SMBC), and the metabolic quotient of microbial biomass in continuous corn (Zea mays L.) under conventional (CT), moldboard (MB), chisel (CH), minimum tillage (MT), and no-tillage (NT) with low (45kgNha–1) and high (90kgNha–1) N fertilization. An Orelia sandy clay loam (fine-loamy, mixed, hyperthermic Typic Ochraqualf) in south Texas, United States, was sampled before corn planting in February, during pollination in May, and following harvest in July. Soil inorganic N, SMBC, and potential C and N mineralization were usually highest in soils under NT, whereas these characteristics were consistently lower throughout the growing season in soils receiving MB tillage. Nitrogen fertilization had little effect on soil inorganic N, SMBC, and potential C and N mineralization. The metabolic quotient of microbial biomass exhibited seasonal patterns inverse to that of SMBC. Seasonal changes in SMBC, inorganic N, and mineralizable C and N indicated the dependence of seasonal C and N dynamics on long-term substrate availability from crop residues. Long-term reduced tillage increased soil organic matter (SOM), SMBC, inorganic N, and labile C and N pools as compared with plowed systems and may be more sustainable over the long term. Seasonal changes in active soil C and N pools were affected more by tillage than by N fertilization in this subhumid climate. Received: 20 September 1996  相似文献   

12.
Vineyard management practices to enhance soil conservation principally focus on increasing carbon (C) input, whereas mitigating impacts of disturbance through reduced tillage has been rarely considered. Furthermore, information is lacking on the effects of soil management practices adopted in the under-vine zone on soil conservation. In this work, we evaluated the long-term effects (22 years) of alley with a sown cover crop and no-tillage (S + NT), alley with a sown cover crop and tillage (S + T), and under-vine zone with no vegetation and tillage (UV) on soil organic matter (SOM), microbial activity, aggregate stability, and their mutual interactions in a California vineyard in USA. Vegetation biomass, microbial biomass and activity, organic C and nitrogen (N) pools, and SOM size fractionation and aggregate stability were analysed. Soil characteristics only partially reflected the differences in vegetation biomass input. Organic C and N pools and microbial biomass/activity in S + NT were higher than those in S + T, while the values in UV were intermediate between the other two treatments. Furthermore, S + NT also exhibited higher particulate organic matter C in soil. No differences were found in POM C between S + T and UV, but the POM fraction in S + T was characterized by fresher material. Aggregate stability was decreased in the order: S + NT > UV > S + T. Tillage, even if shallow and performed infrequently, had a negative effect on organic C and N pools and aggregate stability. Consequently, the combination of a sown cover crop and reduced tillage still limited SOM accumulation and reduced aggregate stability in the surface soil layer of vineyards, suggesting relatively lower resistance of soils to erosion compared to no-till systems.  相似文献   

13.
Soil total organic carbon (TOC) is a composite indicator of soil quality with implications for crop production and the regulation of soil ecosystem services. Research reports on the dynamics of TOC as a consequence of soil management practices in subtropical climatic conditions, where microbial carbon(C) loss is high, are very limited. The objective of our study was to evaluate the impact of seven years of continuous tillage and residue management on soil TOC dynamics (quantitative and qualitati...  相似文献   

14.
Legumes have been shown to increase P uptake of the following cereal, but the underlying mechanisms are unclear. The aim of this study was to compare the effect of legume pre-crops and their residues on the growth, P uptake and size of soil P pools in the rhizosphere of the following wheat. Three grain legumes (faba bean, chickpea and white lupin) were grown until maturity in loamy sand soil with low P availability to which 80?mg P kg?1 was supplied. This pre-crop soil was then amended with legume residues or left un-amended and planted with wheat. The growth, P uptake and concentrations of P pools in the rhizosphere of the following wheat were measured 6?weeks after sowing. In a separate experiment, residue decomposition was measured over 42?days by determining soil CO2 release as well as available N and P. Decomposition rates were highest for chickpea residues and lowest for wheat residues. P release was greatest from white lupin residues and N release was greatest from faba bean residues, while wheat residues resulted in net N and P immobilisation. The growth of the following wheat was greater in legume pre-crop soil without residue than in soils with residue addition, while the reverse was true for plant P concentration. Among the legumes, faba bean had the strongest effect on growth, P uptake and concentrations of the rhizosphere P pools of the following wheat. Regardless of the pre-crop and residue treatment, wheat depleted the less labile pools residual P as well as NaOH-Pi and Po, with a stronger depletion of the organic pool. We conclude that although P in the added residues may become available during decomposition, the presence of the residues in the soil had a negative effect on the growth of the following wheat. Further, pre-crops or their residues had little effect on the size of P pools in the rhizosphere of wheat.  相似文献   

15.
Effects of cropping systems on soil organic matter (SOM) in a pair of conventional and biodynamic mixed cropping farms were investigated. Soil samples (0–75 and 75–150-mm depths) were analysed for total carbon (TC), total nitrogen (TN), microbial biomass C (BC) and microbial biomass N (BN), and sequentially extracted for labile and stable SOM using cold water, hot water, acid mixtures and alkalis. In the biodynamic farm, TC and TN decreased with increasing period of cropping but the reverse occurred under pastures. These were not shown in soils from the conventional farm, probably due to N fertilizer additions. Under pastures, increases in SOM were attributed to greater biological N2 fixation and the return of plant residues and excreta from grazing animals. Overall, sensitive SOM quality indicators found for labile SOM were BN, BN:TN and HC:TC, and for stable SOM were HCl/HFC, HCl/HFC:TC, humin C, humin N, humin C:TC and humin N:TN. The BN and BN:TN were better indicators than BC and BC:TC. The humin fraction was strongly related to both labile and stable SOM fractions suggesting that humin contained non-extractable strongly complexed SOM components with mineral matter and also non-extractable plant and microbial residual components. Received: 10 October 1996  相似文献   

16.
Legume pre-crops may increase P uptake of the following wheat, but the mechanisms behind this effect are unclear. A rotation study was carried out to assess the concentrations of rhizosphere P pools of three grain legumes and wheat (phase 1) and their effects on P uptake and P pools in the rhizosphere of the following wheat (phase 2). Faba bean, chickpea, white lupin and wheat were grown for 10 weeks in a loamy sand soil with low P availability. The following wheat was grown in the pre-crop soil with and without addition of pre-crop residues. Among the pre-crops, white lupin had the strongest effect on the P pools; it depleted the labile P pools, resin P and NaHCO3-Pi and also the less labile P pools, NaOH-Pi and residual P; whereas the concentration of NaHCO3-Po was higher than that in the rhizosphere of the other pre-crops. White lupin had a smaller biomass compared to faba bean which depleted the P pools to a lesser extent. Phosphorus uptake of the following wheat was greatest in white lupin pre-crop soil. Chickpea increased P uptake of the following wheat when residues were added. In the presence of residues, wheat after legumes depleted labile P pools to a greater extent than wheat after wheat, but this coincided with greater P uptake only in wheat after chickpea and white lupin, which may be explained by the small root biomass of wheat after faba bean. The results show that the greater P uptake of the following wheat induced by pre-crops may be due to two mechanisms: P mobilisation (white lupin) or P addition with legume residues (chickpea). This study further showed that P uptake by a crop is only partly a function of the depletion of P in the rhizosphere; another important factor is the ability to exploit a large soil volume.  相似文献   

17.
This study examined the chemical composition of soil organic matter (SOM) along a 2,000-year paddy soil chronosequence in eastern China by use of advanced solid-state nuclear magnetic resonance (NMR) spectroscopy as well as Fourier transform infrared spectroscopy (FTIR), aiming to identify changes in the chemical composition of SOM over a millennium timescale. The results showed that soil organic carbon concentration in the surface soil reached a steady state after 100 years of rice (Oryza sativa L.)–wheat (Triticum sp.) cropping on coastal tidal flats. The 13C NMR spectra and fractions of structural groups or components of the whole SOM samples differed little along the chronosequence, suggesting a similar chemical composition in SOM samples regardless of the duration of rice cultivation. The FTIR spectral pattern and relative intensities of some resolved functional groups or components of whole SOM were also similar along the soil chronosequence. The similarities in chemical composition of SOM can be attributed to the rice–wheat cropping system, in which SOM has undergone ongoing turnover under periodical fresh plant material input and wet–dry cropping alternation, leading to a similar chemical composition of bulk SOM.  相似文献   

18.
A calcareous and clayey xeric Chromic Haploxerept of a long‐term experimental site in Sicily (Italy) was sampled (0–15 cm depth) under different land use management and cropping systems (CSs) to study their effect on soil aggregate stability and organic carbon (SOC). The experimental site had three tillage managements (no till [NT], dual‐layer [DL] and conventional tillage [CT]) and two CSs (durum wheat monocropping [W] and durum wheat/faba bean rotation [WB]). The annually sequestered SOC with W was 2·75‐times higher than with WB. SOC concentrations were also higher. Both NT and CT management systems were the most effective in SOC sequestration whereas with DL system no C was sequestered. The differences in SOC concentrations between NT and CT were surprisingly small. Cumulative C input of all cropping and tillage systems and the annually sequestered SOC indicated that a steady state occurred at a sequestration rate of 7·4 Mg C ha−1 y−1. Independent of the CSs, most of the SOC was stored in the silt and clay fraction. This fraction had a high N content which is typical for organic matter interacting with minerals. Macroaggregates (>250 µm) and large microaggregates (75–250 µm) were influenced by the treatments whereas the finest fractions were not. DL reduced the SOC in macroaggregates while NT and CT gave rise to higher SOC contents. In Mediterranean areas with Vertisols, agricultural strategies aimed at increasing the SOC contents should probably consider enhancing the proportion of coarser soil fractions so that, in the short‐term, organic C can be accumulated. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

19.
Abstract. We compared the effects of conventional and organic arable farming on soil organic matter (SOM) content, soil structure, aggregate stability and C and N mineralization, which are considered important factors in defining sustainable land management. Within one soil series, three different farming systems were selected, including a conventional and an organic arable system and permanent pasture without tillage. The old pasture represents optimal conditions in terms of soil structure and organic matter inputs and is characterized by high earthworm activity. More than 70 years of different management has caused significant differences in soil properties. SOM content, mineralization, earthworm activity and water-stable aggregation decreased as a result of tillage and arable cropping when compared with pasture, but were significantly greater under organic farming than under conventional farming. Total SOM contents between 0 and 20 cm depth amounted to 15, 24 and 46 g kg−1 for the conventional arable, organic arable and permanent pasture fields, respectively. Although less sensitive to slaking than the conventionally managed field, the soil under organic farming was susceptible to compaction when high pressures were exerted on the soil under wet conditions. The beneficial effects of organic farming are generally associated with soil biochemical properties, but soil physical aspects should also be considered. Depending on soil type and climate, organic farmers need to be careful not to destroy the soil structure, so that they can enjoy maximum advantage from their organic farming systems.  相似文献   

20.
The carbon management index (CMI) is derived from the total soil organic C pool and C lability and is useful to evaluate the capacity of management systems to promote soil quality. However, the CMI has not been commonly used for this purpose, possible due to some limitations of the 333 mM KMnO4-chemical oxidation method conventionally employed to determine the labile C fraction. We hypothesized, however, that physical fractionation of organic matter is an alternative approach to determine the labile C. The objectives of this study were (i) to assess the physical fractionation with density (NaI 1.8 Mg m−3) and particle-size separation (53 μm mesh) as alternative methods to the KMnO4-chemical oxidation (60 and 333 mM) in determining the labile C and thus the CMI, and (ii) to evaluate the capacity of long-term (19 years) no-till cropping systems (oat/maize: O/M, oat + vetch/maize: O + V/M, oat + vetch/maize + cowpea: O + V/M + C, and pigeon pea + maize: P + M) and N fertilization (0 and 180 kg N ha−1) to promote the soil quality of a Southern Brazilian Acrisol, using the CMI as the main assessment parameter. Soil samples were collected from 0 to 12.5 cm layer, and the soil of an adjacent native grassland was taken as reference. The mean annual C input of the cropping systems varied from 3.4 to 6.0 Mg ha−1 and the highest amounts occurred in legume-based cropping systems and N fertilized treatments. The C pool index was positively related to the annual C input (r2 = 0.93, P < 0.002). The labile C determined by density (4.4–10.4% of C pool) and particle-size separation (9.5–17.7% of C pool) had a close relationship (r = 0.60 and 0.85, respectively) with the labile C determined using 60 mM KMnO4 (7.3–10.5% of C pool). The labile C resulting from the three methods was related to the annual C input imparted by the cropping systems (r2 = 0.67–0.88), reinforcing the possibility of using physical fractionation as an alternative approach to determine labile C. In contrast, the chemical method using 333 mM KMnO4 was not sensitive to different cropping systems and resulted in too high percentage of labile C, varying from 16.8 to 35.2% of the C pool. The CMI based on physical fractionation was a sensitive tool for assessing the capacity of management systems to promote soil quality, as evidenced by its close correlation (r = 0.88, at average) with soil physical, chemical, and biological attributes. The introduction of winter (vetch) and, especially, summer legume cover crops (cowpea and pigeon pea), or application of fertilizer-N, improved the capacity of the management system into promote soil quality in this subtropical Acrisol.  相似文献   

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