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为探讨亚硒酸钠对黄粉虫幼虫血淋巴谷胱甘肽过氧化物酶（GSH—PX）活性的影响,将黄粉虫的中龄幼虫随机分成6组,饲喂含不同浓度的亚硒酸钠饲料。采用DTNB比色法测定GSH—PX活性。结果显示,饲喂含亚硒酸钠的饲料后黄粉虫幼虫血淋巴GSH—PX活力都明显上升：饲喂同一浓度的亚硒酸钠,幼虫血淋巴GSH—PX活力均比对照组高,只有加硒浓度为20mg／kg．80mg／kg在第16d时,黄粉虫血淋巴GSH—PX活力随着饲喂时间的延长而显著上升;饲喂不同浓度的亚硒酸钠,在硒浓度为5mg／kg、10mg／kg时,黄粉虫血淋巴GSH-PX活力随亚硒酸钠浓度的增加显著上升,在硒浓度为20mg／kg时开始下降。 相似文献
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研究低硒日粮对雏鸡血液中淋已细胞含硒量和谷胱甘肽过氧化物酶活性的影响,选用星布罗雏鸡６０只,分２组,第１组喂低硒基础日粮,第２组喂基础日粮补加０．１５μｇ／ｇ硒。结果,低硒日粮组雏鸡血液中淋巴细胞平均含硒量为（０．０６７１５±０．０１５０）μｇ／ｇ,与对照组含硒量（０．２３７２±０．０４５８）μｇ／ｇ差异显著（Ｐ＜０．０５）;低硒日粮组鸡血液中淋巴细胞的ＧＳＨ－ＰＸ活性（１．１９５２±０．３９７７）单位,活性显著低于对照组（５．３４７８±１．６２７９）单位,差异显著（Ｐ＜０．０５）。 相似文献
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试验旨在研究饲粮中微量元素硒添加水平对笼养蛋雏鸭生长性能、免疫器官发育及抗氧化功能等方面的影响。试验采用单因素完全随机分组设计,选取1日龄健康金定蛋雏鸭180只,随机分为5组,每组6个重复,每个重复6只雏鸭。第Ⅰ组为对照组,饲喂玉米-豆粕型基础日粮(硒含量为0.042 mg/kg),第Ⅱ~Ⅴ组分别饲喂在基础日粮中添加0.1、0.15、0.2、0.3 mg/kg硒(实际添加物为亚硒酸钠)的试验日粮,试验期4周。结果表明:①第Ⅲ组的平均日增重、料重比与对照组相比均差异极显著(P<0.01),且日增重和料重比随饲粮硒含量的增加呈明显的二次曲线变化;②饲粮中添加硒可促进免疫器官发育,但对胸腺指数以外的免疫器官指数影响不显著(P>0.05);③饲粮中添加硒可极显著提高笼养蛋雏鸭GSH-Px活性和T-AOC(P<0.01),降低血清和肝脏MDA含量(P<0.01);④以试验各项测定结果为依据,估测的硒适宜添加水平分别是0.154、0.172、0.180 mg/kg。采用玉米-豆粕型基础日粮蛋雏鸭添加量为0.180 mg/kg时效果最好。 相似文献
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本试验采用低硒玉米、加硒玉米和喷硒玉米为基础日粮对雏鸡进行了多种组织的病理学反应的观察,进一步证明硒对雏鸡预防硒的缺乏及其生长发育的重要作用。 1 实验材料及方法 1.1 实验动物及分组 采用1日龄星布罗肉鸡雏300只,雌雄各半随机分6组,每组各50只。各组饲喂的基础日粮组成相同,添加剂的微量元素、维生素种类和数量相同,但含硒量不同,分为低硒基础日粮组、补硒基础日粮组和喷硒日粮组,详见表1。 相似文献
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本文通过在低硒基础日粮中添加不同水平的维生素E饲喂雏鸡,对维生素E在低硒饲养雏鸡体内的含量和分布规律及其与硒代谢的关系进行了研究。结果表明,日粮维生素E水平可直接影响雏鸡血液和组织维生素E的含量;血液维生素E与组织维生素E含量间呈良好的正相关关系;维生素E在低硒饲养雏鸡各组织器官的含量依次为肝>血液>胰腺>肌肉>脑。在本实验条件下,低硒日粮添加维生素E能影响血硒含量,但组织硒含量则不受日粮维生素E水平的影响。 相似文献
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不同硒水平对生长育肥猪血液生化指标的影响 总被引：5，自引：1，他引：4
试验研究了宁夏常用饲料中不同硒水平对生长育肥猪血液生化指标的影响。结果表明 :(1 )谷胱甘肽过氧化物酶 (GSH PX)活性随饲粮硒水平的增加 ,呈现下降 上升 下降的动态变化趋势。谷胱甘肽硫转移酶可反映缺硒的程度。 (2 )缺硒组仔猪血硒含量和全血中GSH PX均处于缺硒边缘状态 ,在临界值以下 ,不能满足仔猪的代谢需要。 (3)随饲粮硒水平的逐渐增加 ,猪瘟的抗体水平和淋巴细胞转化率明显升高 ,以饲粮中添加硒 0 45mg/kg时达到最高 ,仔猪发病率最低 相似文献
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Supranutritional dietary selenium depressed expression of selenoprotein genes in three immune organs of broilers 
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下载免费PDF全文 Jiayong Tang Xiaofeng Huang Longqiong Wang Qiang Li Jinyang Xu Gang Jia Guangmang Liu Xiaoling Chen Haiying Shang Hua Zhao 《Animal Science Journal》2017,88(2):331-338
The objective of this study was to investigate the effects of supranutritional dietary selenium (Se) on selenoproteins expression in three immune organs of chickens. A total of 160 1‐day‐old male Cobb broilers were randomly divided into two groups and fed a Se‐deficient corn‐soybean basal diet supplemented with 0.3 (adequate) and 3.0 (excess) mg/kg Se for 42 days. Immune organs were collected, and effects of supranutritional Se on messenger RNA abundance of 23 selenoprotein genes and eight inflammation‐related genes were compared at day 42. Also enzyme activities were measured at days 14, 28 and 42. The results showed supranutritional dietary Se depressed growth performance of chicken and down‐regulated nine and three selenoprotein genes in thymus and spleen, respectively, and only Sepp1 was up‐regulated in the bursa of Fabricius. Also three, three and seven inflammation‐related genes were up‐regulated in three organs, respectively. Supranutritional Se elevated (P < 0.05) activities of superoxidase dismutase, total antioxidant capacity and glutathione peroxidase, mainly in early stages. In summary, supranutritional Se resulted in down‐regulation of selenoprotein genes and up‐regulation of inflammation‐related genes in three immune organs of chicken, which indicated potential roles of those selenoprotein genes in immune organs of the chicken. 相似文献
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A low-selenium diet increases thyroxine and decreases 3,5,3'triiodothyronine in the plasma of kittens 总被引：1，自引：0，他引：1
Yu S Howard KA Wedekind KJ Morris JG Rogers QR 《Journal of animal physiology and animal nutrition》2002,86(1-2):36-41
The effect of a low-selenium diet on thyroid hormone metabolism was investigated in growing kittens. Twelve specific-pathogen-free kittens with ages ranging from 16 to 18 weeks were divided into two groups of equal number with equal sex distribution in each group. One group was fed a yeast-based low-selenium diet (0.02 mg Se/kg diet) while the other group was fed the same diet supplemented with Na2 SeO3 at 0.4 mg Se/kg diet for 8 weeks. Food intake, body weight and body weight gain were not affected by the low-Se diet during the study period. However, kittens given the low-Se diet had significantly reduced plasma selenium concentration and glutathione peroxidase activity. Plasma total thyroxine (T4) increased and total 3,5,3'triiodothyronine (T3) decreased significantly in kittens fed the low-Se diet at the end of the study. These results suggest that type I deiodinase in cats is a selenoprotein- or a selenium-dependent enzyme. 相似文献
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SHI Li-guang ZHOU Xiong XUN Wen-juan HOU Guan-yu ZHOU Han-lin HUANG Xian-zhou 《中国畜牧兽医》2016,43(1):101-107
This experiment was conducted to study the effect of selenium and vitamin E supplement on semen quality, antioxidant enzyme activities and heat shock protein expression of goat in Hainan high temperature season.16 adult Hainan Black goat with good health and approximate weight were randomly divided into 4 groups, fed with basal diet(control group), basal diet+0.5 mg/kg Se(Se group), basal diet+100 mg/kg VE(VE group), and basal diet+0.5 mg/kg Se+100 mg/kg VE(Se+VE group), respectively.The experimental period was 93 d.Semen samples were collected in the last week of the experiment on two consecutive days.The semen quality, antioxidant enzyme activities and heat shock protein expression were analyzed.The results showed that compared with control group, the ejaculate volume was not significantly affected by Se or VE supplement(P>0.05).Sperm density and sperm motility were increased significantly by Se and VE supplement(P<0.05), and the abnormal rate was decreased extremely significantly(P<0.01).The goats fed with Se and VE also had higher activities of GSH-Px(P<0.01), SOD(P<0.05), CAT(P<0.05) and T-AOC(P<0.01), and lower MDA concentration in seminal plasma(P<0.05).The relative expression levels of HSP70 and HSP90 mRNA in supplement groups were decreased extremely significantly(P<0.01).However, there were some certain differences between the Se and VE supplement groups on semen quality and heat shock protein expression.In conclusion, the supplementation of Se and VE could help to improve goat semen quality by increasing the sperm density, sperm motility, the antioxidant enzyme activities, and decreasing the abnormal rate in hot season of Hainan.Finally, Se and VE supplement had good effects on relieving the environment heat stress. 相似文献
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补饲硒和维生素E对高温季节山羊精液品质、抗氧化酶活性及热休克蛋白表达的影响 总被引：1，自引：0，他引：1
试验旨在研究在海南夏季高温饲养条件下,山羊补饲硒和维生素E对其精液品质、抗氧化酶活性及热休克蛋白表达的影响。选用16只健康状况良好、体重相近的海南黑山羊成年公羊,随机分成4组,分别饲喂基础日粮(对照组)、基础日粮+0.5mg/kg Se(Se组)、基础日粮+100mg/kg VE(VE组)和基础日粮+0.5mg/kg Se+100mg/kg VE(Se+VE组),试验期93 d。试验期结束前1周采集精液,评价精液品质、测定精浆抗氧化酶活性和精液热休克蛋白表达。结果表明,与对照组相比,补饲Se和VE对海南黑山羊射精量影响差异不显著(P>0.05);补饲Se和VE能显著提高海南黑山羊的精子密度和精子活力(P<0.05),极显著降低精子畸形率(P<0.01);极显著增加精浆谷胱甘肽过氧化物酶活性和总抗氧化能力(P<0.01),显著增加超氧化物歧化酶和过氧化氢酶活性(P<0.05),显著降低丙二醛浓度(P<0.05);补饲Se和VE还极显著降低了精液HSP70和HSP90mRNA的表达水平(P<0.01),但各补饲组之间对精液品质改善效果和热休克蛋白表达丰度的影响存在一定差异。综上所述,补饲Se和VE有助于提高热带地区夏季高温季节山羊精子密度、精子活力,降低畸形率,同时还能增强精浆中抗氧化酶的活性,改善精液品质,进而起到缓解环境热应激的效果。 相似文献
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Yea Hwang MOON Ji Un OK Shin Ja LEE Jong Kyu HA Sung Sill LEE 《Animal Science Journal》2010,81(6):642-647
A comparative study among Korean native cow (Hanwoo), Holstein dairy cow, Korean native goat and crossbred sheep on the population and marker concentration of ruminal microbes, the activities of carboxymethylcellulase (CMCase), xylanase and amylase, and in situ dry matter (DM) degradability were conducted. Twelve ruminally cannulated animals, three of each species, were used. Animals were fed the same diet containing 40% formula feed and 60% rice straw at the level of 2.5% of body weight. Total viable microbial populations in the rumen fluid were significantly (P < 0.01) greater for bacteria and fungi in goat than those of Holstein. The protozoan population among ruminant species was the reverse from that of bacteria. The concentrations of 2,6‐diaminopimelic acid and chitin as markers for bacteria and fungi in the rumen fluid, respectively, were highest in goat, which is in accordance with the above population data. The concentration of aminoethylphosphonic acid as marker of protozoa was highest in Hanwoo and lowest in sheep (P < 0.01). Goat had the highest (P < 0.01) activities of all the enzymes investigated among ruminants. In situ effective degradation of the DM of rice straw was approximately 19% higher in the rumen of goat compared with other animals. 相似文献
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硒酵母对西门塔尔牛日粮养分消化代谢和血清生化指标的影响 总被引：1，自引：0，他引：1
本试验旨在研究硒酵母对西门塔尔牛营养物质消化代谢和血清生化指标的影响。选用16头平均体重420 kg,年龄2.5岁的西门塔尔牛阉牛,采用随机区组设计分为4组,以硒酵母为硒源,分别在日粮中添加硒0、0.3、0.6和0.9 mg/kg。结果表明:日粮养分表观消化率、矿物元素表观存留率、消化氮和沉积氮/消化氮比例均以0.3 mg/kg与0.6 mg/kg组较高(P<0.05);0.6 mg/kg组甘油三酯显著提高,处理组血糖显著增加,0.6 mg/kg和0.9 mg/kg组白蛋白增加(P<0.05),总胆固醇、总蛋白和尿素氮差异不显著(P>0.05);0.6 mg/kg和0.9 mg/kg组血清GOT和GPT含量显著提高(P<0.05),AKP、ACP和LDH差异均不显著(P>0.05);处理组GSH-px活性显著提高(P<0.05),以0.6 mg/kg组最高;0.6 mg/kg和0.9 mg/kg组MDA显著降低(P<0.05);0.6 mg/kg组GSH含量显著增加(P<0.05);0.6 mg/kg和0.9 mg/kg组血清硒含量显著增加(P<0.05)。日粮以硒酵母为硒源时,添加硒0.6 mg/kg显著促进营养物质消化代谢和提高机体抗氧化能力,建议日粮加硒为0.6 mg/kg。[动物营养学报,2007,19(4):379-385] 相似文献
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The investigation comprised 12 herds, totalling 660 sheep, in the counties of Stockholm and Uppsala. The selenium content of specimens of forage, whole blood, muscle, liver, and kidney was determined by a fluorimetric method. Data on the number of born and dead lambs were collected 4—5 months after lambing. Dead and sick lambs were subjected to necropsy and to clinico-chemical analyses, respectively.The selenium content of hay and corn was lower than 60 ng per g, which some authors regard as the minimum level of Se requirement for protection against white muscle disease in sheep. The analyses of forage mixtures containing oil concentrates and beet pulp showed selenium levels that were about 2—5 times as high as this minimum level.In herds in which the animals had not been treated with selenium preparations prior to sampling, there was a significant positive correlation between the selenium content of the forage and that of whole blood. A relationship was also noted between the selenium content of forage and of organs.In 9 out of the 12 herds the selenium concentrations in whole blood were on the level that is considered to be representative of white muscle disease.A high mortality rate was recorded for lambs whose mothers had low selenium levels in their blood.Prophylactic and curative treatment with selenium plus vitamin E orally and/or parenterally probably reduced lamb mortality rate in the herds fed a low-selenium diet. 相似文献
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The effect of dietary selenium (Se) deficiency on the generation of neutrophil chemotactic factors from goat polymorphonuclear leukocytes (PMN) was studied. Polymorphonuclear leukocytes were isolated from 2 groups of crossbred goats. One group (n = 3) was fed a diet deficient in Se; the other (n = 3) was fed a diet adequate in Se. The PMN from each goat were stimulated with calcium ionophore A23187 and supernatants were collected. Each supernatant was immediately assayed for its ability to elicit neutrophil chemotaxis (NCT) and neutrophil chemokinesis by the Boyden chamber technique and neutrophil chemiluminescence (NCL) by the luminol-dependent chemiluminescence assay. Measurements from each group were compared, using the Student's t test. There was no significant difference (P greater than 0.01) in neutrophil chemokinesis elicited by supernatants derived from stimulated PMN from the 2 groups. However, supernatants derived from PMN of goats fed the Se-deficient diet had a significantly decreased capacity (P less than 0.01) to stimulate NCT and NCL. In vitro incubation of PMN with Se before ionophore treatment produced supernatants with increased NCL stimulation (P less than 0.05), but none with increased NCT stimulation (P greater than 0.05). However, both activities were restored after goats fed the Se-deficient diet (P less than 0.01) were given Se subcutaneously. Seemingly, dietary Se deficiency is associated with depressed PMN generation of factors that stimulate NCT and NCL. In contrast to NCL factors, synthesis of factors stimulating NCT probably was not associated directly with PMN glutathione peroxidase concentration. 相似文献
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A. S. Mueller J. Fischer E. Most J. Pallauf 《Journal of animal physiology and animal nutrition》2009,93(3):313-324
To evaluate dietary selenium (Se) requirement in turkeys offered a diet supplemented with two levels of vitamin E (VE), 96 newly hatched male BIG 6® chicks (58.4 ± 4.12 g) were divided into eight groups of 12 animals each and fed maize soya diets containing 0.05, 0.10, 0.20 and 0.30 mg Se/kg from sodium selenate in combination either with the natural VE content (approximately 10 IU/kg) or with a VE addition of 50 IU/kg. Animals from all the groups were highly performant and their final body weights (1746 ± 190 g) after 35 days on experiment were not significantly different. According to its dietary supply, Se concentration in the liver and plasma increased dose dependently. Independent of dietary VE, the activities of GPx3 in plasma and of GPx1 in liver and breast muscle increased to a larger extent in turkeys supplemented with 0.10 and 0.20 mg Se/kg in relation to animals with low marginal Se supply (0.05 mg/kg). Supplementation of 0.30 mg Se/kg only slightly increased further selenoprotein activities. 2‐Thiobarbituric acid reactive substances in the liver were strongly reduced by dietary VE, but not by Se. Plasma aspartate aminotransferase (AST) and creatine kinase (CK) activities did not show muscular lesions in none of the groups. Although there were no signs of muscular lesions even in turkeys with marginal Se and moderate VE supply, the activity of selenoproteins in various organs increased up to 0.30 mg Se/kg diet, independent of VE supply. It was concluded that for growing turkeys the Se supply should meet at least a level of 0.20 mg/kg diet as currently recommended by the National Research Council and Gesellschaft für Ernährungsphysiologie. Vitamin E addition confirmed the particular function of the vitamin as a lipid antioxidant and should be taken into consideration when diets with high PUFA concentrations are fed. 相似文献
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选用6月龄海门山羊羯羊40只,随机分为4组,每组10只。其中,3个为试验组,1个为对照组,进行不同饲喂量的短期肥育试验。结果表明:试验补饲组的增重水平和肥育效益明显优于对照组,其中以试验2组增重效果和肥育效益最好,试验3组虽然增重效果好但是效益下降。试验2组日增重达到0.81kg,比对照组0.49kg提高65.3%;60天肥育收入和日收入分别为389.3元和6.5元比对照组的294.0元和4.9元分别多收入95.3元和1.6元,提高32.7%。表明海门山羊短期补饲肥育能提高日增重,缩短养羊周期,增加养羊经济效益。 相似文献