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A live attenuated auxotrophic S. typhimurium (S. tm.)-mutant was used by orally administration via drinking water several times during rearing, combined with 1- or 2-times parenteral injection of an autogenous S. enteritidis (S. e.)/S. tm.-oil emulsion vaccine. In a 8-month period, more than 500,000 birds were vaccinated. The vaccine was safe. Challenge test showed protection in the vaccinates and their offspring. The number of isolates in the farms detected by regular monitoring decreased. The protection of the live-culture mutant lasted about only 8 to 10 weeks. A. S. tm.-mutant more potent for chickens will be tested now. We consider vaccinations as one important factor in a salmonella control program, especially for commercial layers and broiler breeders.  相似文献   

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After multiple infections with S. enteritidis in humans and demonstration of S. enteritidis in egg products, S. enteritidis (lysotype 6, plasmid profile 40) could be isolated from organs of hens, anal swab tests of chicken, liquid manure, egg shells and non pasteurized egg contents. Because of the largeness of the hen farm prophylactic vaccinations seemed to be advisable additionally to improvement of the management and hygiene. A vaccine registered in 1987 for use in pigeons and water fowl "Zoosal oral Dessau" was used. As a test three were vaccinated three times and artificially infected. Cross immunity against S. enteritidis could be demonstrated. In October 1987 all chicken of the farm were vaccinated three times at the age of 4, 6 and 7 weeks; random samples were tested for immunity and cross immunity. Until the end of the laying period immunity against S. typhimurium could be proven in 98% of the hens, against S. enteritidis in 95%.  相似文献   

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In three experiments a solution of formic acid (Bioadd) was added to feed 'naturally' contaminated with salmonellas. In two of them no salmonella infections were demonstrated in broiler chickens given feed containing 0.6 per cent (w/w) of the formic acid solution for seven weeks and in the third the infection rate was reduced considerably. In four other experiments the feed was contaminated artificially with between 1 and 47 Salmonella kedougou/g and a second product (BPO12), consisting of a mixture of formic acid and propionic acid, was evaluated. When it was added at 0.5 to 0.68 per cent (w/w) only one of 30 groups of 10 to 12 birds became infected with S kedougou by two weeks of age, compared with 22 of 27 control groups. The treatment of the feed with BPO12 one week before the addition of the salmonellas prevented the establishment of infection in chicks given the treated feed, indicating that acid treatment will provide protection against subsequent recontamination of the feed with the organisms. The isolation rate of S kedougou from artificially contaminated feed treated with BPO12 was reduced only slightly but the feed was apparently 'safe' for the birds which consumed it.  相似文献   

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Oral salmonella antigens for the control of salmonella in chickens   总被引:4,自引:0,他引:4  
Multivalent antigen preparations of sonicated lyophilized cells derived from up to 6 different salmonella serotypes were added in decreasing levels to broiler feed throughout the broiler's life. Several preliminary experiments challenging chicks via contaminated feed with some of the homologous serotypes showed that salmonellae were cleared more rapidly from treated chicks than from untreated controls and that fewer isolations of salmonellae were made from their ceca.  相似文献   

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One hundred and thirteen finishing pig units and 74 sow units in Catalonia, Spain, were examined to determine the prevalence of salmonella infections and the factors that could be associated with them. Pooled faecal samples were taken from the finishing units, and samples of faeces were collected from individual sows. The Salmonella isolates were serotyped, phage typed and examined for their antimicrobial susceptibility to 18 common antimicrobial drugs. In addition, blood samples from pigs on 141 farms were analysed by ELISA. In both the bacteriological and serological surveys, a questionnaire with 84 questions was completed for each farm. Salmonella species were isolated from 20 per cent of the finishing units and 24 per cent of the sow units; 14 serotypes were detected in the finishing pigs and 11 in the sows. More than 30 per cent of the strains were resistant to tetracycline, sulphonamides, ampicillin or streptomycin, and 69 per cent of the strains were resistant to three or more agents up to 10 compounds. Seventy-seven per cent of the farms had at least one seropositive animal, and 26 per cent of these farms had an individual seroprevalence of 50 per cent or more. The factors associated (P<0.05) with the excretion of Salmonella species in the finishing units were the practice of raising livestock other than pigs (odds ratio [OR]=6.18), the herd size (OR=5.87), and a past history of clinical salmonellosis (OR=4.97). For the sows, the factors associated (P<0.05) with the excretion of Salmonella species were having open-flushed drainage of sewage (OR=34.48), a lack of rodent control measures (OR=0.05) and the number of sows in the unit (OR=9.26). Factors associated with seropositivity in the finishing units were a lack of bird-proof nets (OR=0.30) and the use of water from private wells (OR=3.64).  相似文献   

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美国肉鸡生产行业应该制定一套可行的策略,以解决肠炎沙门氏菌(Salmonella Enteritidis,SE)问题,从而避免美国农业部食品安全检验署(The Food Safety and Inspection Service,FSIS)的严厉处罚措施.  相似文献   

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禽沙门氏菌的检测与防制   总被引:4,自引:0,他引:4  
沙门氏菌是革兰氏阴性、细胞内寄生的一种肠道菌。该菌广泛存在于自然界中,不但能引起家畜家禽及其他动物发生急性、慢性或隐性感染,而且还能通过污染食物导致人的食物中毒,对人类造成很大的威胁。据统计,目前世界上85%的食物中毒是由沙门氏菌引起的。在美国,每年有约2~4百万个沙门氏菌中毒病例,造成约有1000人死亡;美国政府每年投入几十亿美元用于沙门氏菌的检测与防制。以往,人们把对付沙门氏菌的工作认为是养殖业主的事,把减少沙门氏菌对养殖业造成的损失看作唯一的目的。而今,随着社会的发展和人类文明的进步,人们…  相似文献   

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2010年8月中旬,因肠炎沙门氏菌污染问题而发生鸡蛋召回事件之后,美国禽蛋行业陷入了极度不利的公众视线之中.由于禽蛋消费量的立即下降,媒体关注引发的即刻效应是因禽蛋消费量的大幅减少而导致的鸡蛋价格急剧下降,正如20世纪80年代后期英国的"Eggwina"事件一样,当时一位资历较浅的卫生大臣对受污染的鸡蛋向消费者发出警告,导致鸡蛋销售量迅速下降.  相似文献   

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Intracutaneous injection of a crude supernatant fraction from homogenised Salmonella typhimurium SVA 44 (O 4, 5, 12) or S dublin SVA 47 (O 9, 12) elicited highly significant (P less than 0.005) double skin-fold thickness increases in calves spontaneously infected with salmonella and verified as excretors. The use of isolated structurally defined outer membrane components from salmonella bacteria established that the delayed skin reactions could be elicited by either the lipopolysaccharide which contains O-antigenic polysaccharide chains homologous to the infecting strain, or an outer membrane protein fraction (porin). The porin preparation gave rise to skin reactions regardless of which salmonella serotype the calf was infected with. Histological examination of biopsy material indicated a delayed skin reaction. No such reactions were seen in biopsies from control calves. The use of lipopolysaccharide permitted a salmonella serogroup specific skin test although the endotoxic side effects were marked in doses above 50 micrograms. Purified O-antigen specific polysaccharides devoid of lipid A from S typhimurium (O 4, 12) or S enteritidis (O 9, 12) failed however to elicit skin reactions. Infected calves had humoral antibody titres against the O antigen of the infecting strain which were significantly (P less than 0.005) higher than those found in control calves.  相似文献   

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优优 《饲料广角》2011,(5):37-38,40
尽管2010年美国沙门氏菌感染稳居新闻头条的时期已经告一段落,但美国蛋品工业仍未平静,且已经坚定将这些细菌纳入需进行防控的范围之内。Dr Simon Shane博士2010年8月中旬,因肠炎沙门氏菌鸡蛋召回事件之后,美国蛋品工业陷入了极端负面的公众影响之中。媒体关注的即时效应,  相似文献   

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Background

The impact of salmonella contaminated feed ingredients on the risk for spreading salmonella to pigs was assessed in response to two incidences when salmonella was spread by feed from two feed mills to 78 swine producing herds.

Methods

The assessment was based on results from the salmonella surveillance of feed ingredients before introduction to feed mills and from HACCP - based surveillance of the feed mills. Results from the mills of the Company (A) that produced the salmonella contaminated feed, were by the Chi. Square test compared to the results from all the other (B - E) feed producers registered in Sweden. Isolated serovars were compared to serovars from human cases of salmonellosis.

Results

Salmonella (28 serovars) was frequently isolated from imported consignments of soybean meal (14.6%) and rape seed meal (10.0%). Company A largely imported soybean meal from crushing plants with a history of unknown or frequent salmonella contamination. The risk for consignments of vegetable proteins to be salmonella contaminated was 2.4 times (P < 0.0006) larger for A when compared to the mills of the other companies which largely were supplied by soybean meal from a crushing plant with a low risk for salmonella contamination. Also the level of feed mill contamination of salmonella was higher for feed mills belonging to Company A in comparison to the other companies before and also after heat treatment. Four (10.5%) of the 38 serovars isolated from feed ingredients (28) and feed mills (10) were on the EU 2007 top ten list of human cases of salmonellosis and all but eight (78.9%) on a 12 year list (1997-2008) of cases of human salmonellosis in Sweden.

Conclusions

Salmonella contaminated feed ingredients are an important source for introducing salmonella into the feed and food chain. Effective HACCP-based control and associated corrective actions are required to prevent salmonella contamination of feed. Efforts should be taken to prevent salmonella contamination already at the crushing plants. This is challenge for the EU - feed industry due to the fact that 98% of the use of soybean/meal, an essential feed ingredient, is imported from crushing plants of third countries usually with an unknown salmonella status.  相似文献   

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