Received December 27, 2014; accepted October 23, 2015. 相似文献
Received October 14, 2015; accepted June 1, 2016 Published online September 29, 2016 相似文献
This is the first study to reveal gill lesions typical for columnaris disease at macroscopic, light microscopic, and ultrastructural levels in both Common Carp and Rainbow Trout following a challenge with F. columnare. The results provide a basis for research opportunities to examine pathogen–gill interactions.
Received January 10, 2014; accepted July 27, 2014 相似文献
Received November 10, 2014; accepted December 5, 2015. 相似文献
2. The analytes were extracted from yolk, albumen and whole egg by phosphate buffer and ethyl acetate. Following purification, samples were analysed by high-performance liquid chromatography.
3. Fifty laying hens were divided into 5 groups, and each hen received doses of 20 mg/kg FF: Group 1 (received a single oral dose by gavage); Group 2 (a single intramuscular dose); Group 3 (a single subcutaneous dose); Group 4 (multiple oral doses for 3 d) and Group 5 (multiple oral doses for 5 d).
4. Limits of detection and of quantitation values were 1.94 and 6.45 g/109 g (ppb) for FF, respectively, and 0.48 and 1.58 ppb for FFA, respectively. Relative standard deviation values of intra-day and inter-day variation below 11% also confirmed the usefulness of the method for analysing FF and FFA in eggs.
5. From the first day of both oral and parenteral administration, FF and FFA were detected at 0.1% and 0.08% of dosage, respectively, and 57% of the drugs were eliminated from the egg yolk. Elimination time of FF was 8 d in Groups 1, 2 and 3; 9 d in Group 4 and 10 d in Group 5. 相似文献
Received May 6, 2014; accepted October 24, 2014 相似文献
2. Lesions typical of gizzard erosion could be produced by the addition of histamine to the diet.
3. The amount of histamine occurring naturally in fish meal depends on the species of fish and the extent and nature of bacterial spoilage.
4. These variables may explain why histamine has not been implicated previously and also why there have not been consistent associations between the condition and geographical source or common factors in bulk consignments of the meal. 相似文献
Received May 24, 2015; accepted March 24, 2016 Published online August 2, 2016 相似文献
2. Broilers with high HW were heavier than those with low HW during the 21 d assay, which appeared to result from increased body weight gain rather than improved feed conversion efficiency. A higher dietary concentration of methionine (5.9 g/kg) improved growth performance of broilers with low HW in terms of body weight gain and feed conversion ratio.
3. Broilers with different HW had similar antioxidant status both in serum and liver.
4. Broilers given a diet containing 5.9 g/kg methionine had enhanced serum superoxide dismutase (SOD) activity and decreased hepatic malondialdehyde (MDA) content at day 7.
5. Broilers given a diet containing 5.9 g/kg methionine had a higher hepatic reduced glutathione (GSH):glutathione disulphide (GSSG) ratio than those given a diet containing 4.9 g/kg methionine at day 21. High dietary methionine concentration reduced hepatic GSH content and glutathione peroxidase (GPX) activity of broilers with high HW at day 7 and at day 21, respectively, but increased hepatic GSH content of broilers with low HW at day 7.
6. Although broilers with different HW had similar oxidative status as indicated by several parameters in blood and liver, HW can have positive effects on the subsequent growth performance of broilers, and a higher dietary methionine concentration (5.9 g/kg) can improve growth performance and antioxidant status in broilers exhibiting low HW. 相似文献
2. In fresh fish processed to whole fish meal the biological availability of selenium was estimated as 77%, in fresh fish processed to pressed‐cake fish meal it was 59% and in partly‐decomposed fish processed to whole fish meal it was 51%.
3. When the phosphorus content of the test diet was 2–51 g/kg the biological availability was 88% and unaffected by the condition of the fish and the method of processing. 相似文献
Received August 22, 2015; accepted February 13, 2016 相似文献
2. The difference between the NPU values, 0.66 for yeast and 0.80 for the soyabean meal and fish meal mixture, could largely be attributed to the high nucleic acid content of the yeast.
3. Chicks given the diet containing yeast (190 g/kg) did not grow as rapidly as those given the soyabean meal and fish meal reference diet, and the reduced growth could only partly be explained by a marginal deficiency of methionine.
4. Food conversion efficiency with the yeast diet was improved by maize oil while responses to α‐tocopheryl acetate and sodium selenite were inconsistent.
5. Chicks grew well when yeast replaced fish meal in the mixture of soyabean meal and fish meal, and when fish meal (194 g/kg diet) was the sole protein supplement. 相似文献
2. Supplementation with hesperidin did not affect egg production, egg weight and egg quality traits.
3. No hesperidin effect on yolk and plasma cholesterol was observed. A strain effect was found with lower total and per g yolk cholesterol of brown hens in comparison to the white ones.
4. Oxidative stability of egg yolk, expressed as ng MDA/g yolk, was significantly improved in the hesperidin groups even from the first week of supplementation. At the same time, a significant improvement in the oxidative stability of egg yolk due to the incorporation of hesperidin in hens’ diet was observed after 30 and 90 d of storage at 20°C and 4°C, respectively.
5. No hesperidin by strain interaction was detected for any of the traits measured.
6. In conclusion, incorporation of hesperidin to laying hens’ feed did not affect productive and egg qualitative traits. On the other hand, dietary hesperidin supplementation significantly improved oxidative stability of both fresh and stored eggs. Antioxidant properties of hesperidin seem to make it a promising natural agent for improving the shelf life of eggs. 相似文献
2. Plasma α‐tocopherol was significantly correlated with α‐tocopherol concentrations in thigh and breast muscle.
3. The fatty acid profiles of muscle lipids reflected dietary fatty acid composition.
4. The consumption of heated sunflower and linseed oils reduced α‐tocopherol status, altered fatty acid composition of muscle lipids and increased susceptibility of muscle to lipid oxidation.
5. Supplementation of diets containing heated oils with α‐tocopheryl acetate resulted in some alleviation of these effects.
6. The results indicate that caution should be exercised in the use of thermally oxidised oils in poultry diets if undesirable changes in composition and oxidative stability of carcase lipids are to be avoided. 相似文献
2. Broilers of 0.68, 1.27, 2.45 and 5.13 kg were administered FF in a single intravenous dose of 30 mg/kg body weight. Plasma drug concentrations were determined using high-performance liquid chromatography and PK parameters were calculated using a non-compartmental model. Echocardiography was used to measure haemodynamic functions.
3. During growth, the area under the drug concentration-time curve (AUCinf) increased from 25.7 ± 2.9 to 39.0 ± 8.0 mg h/l. Total body clearance (ClB) gradually decreased from 1.19 ± 0.14 to 0.80 ± 0.15 l/h/kg. Elimination half-life increased from 0.73 ± 0.08 to 1.07 ± 0.07 h, whereas volume of distribution (Vss) remained unchanged. Haemodynamic measurements revealed an increase in cardiac output, from 495 ± 65 to 1303 ± 306 ml/min, in the respective body weight groups.
4. Allometric models for PK and haemodynamic parameters were developed and validated. All models proved to be statistically significant; however, only models for ClB and Vss met stringent validation criteria. Model for ClB was used to calculate an optimal dose for a given age group that provides uniform AUCinf.
5. Age-dependent change in FF kinetics may cause variability in therapeutic response under clinical conditions. A novel approach to the dosing protocol was proposed as a means of optimising therapeutic efficacy. 相似文献
In the first experiment a basal soyabean meal diet (14.3 per cent crude protein) was supplemented with L‐lysine and DL‐methionine, singly and together. Supplementation of the basal soyabean diet with the amino acids caused no significant effects on egg production, mean and total egg weight or food conversion efficiency. None of the soyabean diets proved as satisfactory for egg production or food conversion efficiency as a fish meal control diet (16.9 per cent crude protein).
In the second experiment the effect of adding 0.1 per cent each of L‐lysine and DL‐methionine or 0.2 per cent each of l‐lysine and DL‐methionine to a low fish meal diet (2.5 per cent fish meal) was examined. The addition of 0.2 per cent of each amino acid caused an increase in egg production.
Mean egg weight was improved by the addition of amino acids at both levels although this effect was not statistically significant. Comparison of the low fish meal diets with a fishmeal control diet (10 per cent fishmeal) showed that at the 0.2 per cent addition of amino acids, egg production, total egg weight and food conversion efficiency were better than on the 10 per cent fish meal control diet.
Two breeds, light and medium hybrids were used in both experiments.
The results are discussed with reference to their practical significance. 相似文献
2. Hens losing 0.30 of their initial body weight (BWL) produced more hatching eggs and chicks than those which lost 0.20 BW.
3. Hens fed on cracked grain produced fewest non‐hatching eggs, most chicks and the most efficient food utilisation.
4. The interactions showed that hens which lost 0.20 BW and were fed on layers ration produced most eggs. When number of chicks hatching was the criterion then hens losing 0.25 to 0.30 BW which were fed on cracked grain for 3 weeks gave the best performance. 相似文献
2. Urea as a protein substitute does not enhance the value of conventional broiler diets.
3. Urea does not improve broiler diets that have been supplemented with fish meal or crystalline amino acids to satisfy requirements for all indispensable amino acids.
4. Urea is absorbed into the bloodstream, but is not assimilated into body proteins. 相似文献
2. The enzyme in cytosol was separated from ‘classic’ tetrameric glutathione peroxidase and glutathione S‐transferases by DEAE‐Sephacel and Sephadex G‐100 chromatographies and further purified by Mono Q, hydroxylapatite and sulphobro‐mophthalein‐S‐glutathione‐agarose chromatographies.
3. The molecular weight of the purified enzyme determined by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis was 19,500 and that found by gel filtration chromatography was comparable. This indicates that the enzyme protein is a single polypeptide. The isoelectric point of the enzyme was determined as 7.0 by polyacrylamide gel isoelectric focusing.
4. The purified enzyme catalysed the reduction of hydrogen peroxide, cumene hydroperoxide, tert‐butyl hydroperoxide and linoleic acid hydroperoxide. Furthermore, it reduced phosphatidylcholine hydroperoxide in the absence of phospholi‐pase A2. The optimum pH for the enzyme reaction was 7.0. The antiserum against the purified enzyme reacted with the 19.5 kDa polypeptide in the liver cytosol of duck and quail. 相似文献