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1.
本试验采用CELL-DYN1500型血细胞计数分析仪及日立7170全自动生化分析仪对雌雄成年猫血液生理生化值进行测定,并进行统计分析和差异显著性检验.雌雄血液生理生化值无显著性差异(P>0.05).本文建立了健康家猫的血液学及生化指标的正常值范围,可为应用该动物进行科学研究时提供参考.  相似文献   

2.
生活在我国的家养鸭品种很多,品种不同或同一品种生活的地域不同,其血液生化学值是有区别的。截至目前,我国对家鸭血液生化值研究主要集中在以下几个方面。  相似文献   

3.
常规采取9只临床检查健康的灰大袋鼠血样,应用血细胞计数仪和全自动血液生化仪,进行了17项血常规检查和31项血液生化指标测定与分析,填补了国内空白。  相似文献   

4.
实验动物采血方法   总被引:4,自引:0,他引:4  
实验研究中,经常要采集实验动物的血液进行常规检查或某些生物化学分析,故必须掌握血液的正确采集、分离和保存的操作技术。采血方法的选择,主要决定于实验的目的所需血量以及动物种类。凡用血量较少的检验如红、白细胞计数、血红蛋白的测定、血液涂片以及酶活性微量分析法等,可刺破组织取毛细血管的血。当需血量较多时可作静脉采血。静脉采血时,若需反复多次,应自远离心脏端开始,以免发生栓塞而影响整条静脉。例如,研究毒物对肺功能的影响、血液酸碱平衡、水盐代谢紊乱,需要比较动脉血氧分压、二氧化碳分压和血液pH值以及K^+、Na^+、Cl^-离子浓度,必须采取动脉血液。  相似文献   

5.
对鸡粪喂猪的适口性、日增重、肉品质的影响研究报道的较多,但对仔猪喂鸡粪的日增重和血液生化指标研究报道很少。试验在仔猪的基础日粮中添加10%的鸡粪,探讨添加鸡粪对仔猪的日增重和血液生化指标的影响,为鸡粪在仔猪生产上应用提供依据。  相似文献   

6.
通过测定和分析自发性糖尿病猕猴的血液学、生化指标,为建立猕猴自发性糖尿病模型提供参考数据。对发生类似人类糖尿病“三多一少”症状的1只猕猴,应用全自动血细胞分析仪和血液生化分析仪,测定其血液学、生化值,并进行糖耐量试验。结果血糖(空腹)、尿糖和尿素显著升高;甘油三酯、肌酐、总胆固醇、氯、磷、镁和二氧化碳处于正常值高限或增多,血液学中性粒细胞百分比、血红蛋白、平均红细胞血红蛋白和平均红细胞血红蛋白浓度升高。表明猕猴为典型的自发性糖尿病动物。  相似文献   

7.
1高温对蛋鸡机体产生影响 在热环境下,持续的热环境对蛋鸡机体免疫系统有一定影响。高温会明显抑制血液血细胞吞噬细胞的活力,降低血清溶菌杀菌能力。高温下肌肉能量供应不足,造成营养不良,对蛋鸡的生活力和生产性能造成很大影响。热应激下蛋鸡血浆中的二氧化碳分压下降,血浆中的碱储下降,pH 值会上升,体内酸碱平衡失调,机体反馈性地分泌大量有机酸来降低血液pH值。有机酸与钙离子大量结合,从而造成血液中钙离子浓度大大降低,对蛋壳品质造成严重影响。  相似文献   

8.
1热应激对鸡只的危害 干扰鸡只的正常生理功能。热应激可使鸡的心跳速率增加,血压下降,呼吸加快,喘息,严重时可出现呼吸性碱中毒,血液pH值上升,血钠升高,血钾下降,钠钾比例失调(可促使心脏兴奋性增强),血液中葡萄糖、总蛋白质、球蛋白及白蛋白浓度均降低。  相似文献   

9.
《中国家禽》2009,31(9)
近来,密西西比州立大学科学家研究了蛋鸡产蛋前期单独接种ts-11株鸡败血支原体(ts-11MG)疫苗或者与F株鸡败血支厚体(FMG)疫苗联合接种对商品蛋鸡消化器官、繁殖器官、血液特性的影响。试验鸡只的免疫接种方式采取以下4种处理:10周龄,假疫苗;10周龄,tS-11MG疫苗;10周龄,ts-11MG,22周龄,FMG;10周龄时,ts-11MG,45周龄时,FMG。结果显示,除了相对阴道长度,  相似文献   

10.
动脉血压是指血液在动脉管内流动时对血管壁的压力,是由于每次心室收缩所产生的推动血液前进的力量和血液流经动脉时所遇到的外周阻力,这两种相反的力量互相作用形成的。测量血压的变化,在临床上有着重要的意义,可对疾病的临床诊断、护理及预后提供可靠的依据,在医学界已得到广泛应用。但不同方法、畜种及部位所测的结果不一样,因而应建立不同方法、畜种的正常值。目前在兽医上,只建立了部分家畜的血压正常值。骡  相似文献   

11.
为了研究在不同季节各月龄阶段安格斯牛的血液生理与生化指标变化,了解安格斯牛在新疆石河子地区的适应性,该试验分别于冬夏两季,在初生、10月龄、18月龄3个阶段各随机选取50头牛,分别于颈静脉与尾静脉采血进行生理生化指标分析。结果表明:初生阶段,总蛋白含量、血糖浓度、白细胞总数和红细胞数量在冬夏两季差异显著(P<0.05);10月龄阶段,甘油三酯浓度、尿素氮浓度、红细胞数量与白细胞总数在冬夏两季差异显著(P<0.05);18月龄阶段,甘油三酯浓度与白细胞总数在冬夏两季有显著差异(P<0.05);其余指标差异均不显著。安格斯牛对不同气候有相对较好的适应性,但夏季高温可能会影响安格斯牛的生产性能。  相似文献   

12.
本试验通过研究吉林梅花鹿不同生长阶段血液生理生化指标的差异情况,旨在为梅花鹿的健康检测、疾病防治、育种等提供依据和参考。选取吉林省左家、双阳、东丰地区哺乳仔鹿93头(公37头,母56头)、育成鹿135头(公40头,母95头)、成年鹿130头(公42头,母88头),共358头,进行13项血液生理指标、19项生化指标检测。结果显示:哺乳公鹿与哺乳母鹿各项指标总体上差异不大,仅红细胞系统指标、血糖、肌酸激酶具有显著性差异(P<0.05);红细胞压积、红细胞平均体积、平均血红蛋白含量、平均血红蛋白浓度、碱性磷酸酶、乳酸脱氢酶在不同性别的育成鹿与成年鹿中差异极显著(P<0.01)。不同生长阶段公鹿天门冬氨酸氨基转移酶、前白蛋白、总胆汁酸、血糖等差异显著(P<0.05),红细胞系统、肌酸激酶、钙、无机磷、肌酐等差异极显著(P<0.01),白蛋白、淀粉酶差异不显著(P>0.05);不同生长阶段的母鹿淋巴细胞总数、红细胞总数、中性粒细胞总数差异显著(P<0.05),白细胞总数、红细胞系统指标、天门冬氨酸氨基转移酶、丙氨酸氨基转移酶、肌酸激酶、钙、无机磷等差异极显著(P<0.01),谷氨酰转肽酶、总胆汁酸、白蛋白差异不显著(P>0.05)。以上结果说明,不同生长阶段、不同性别的梅花鹿血液生理生化指标存在较大变异,部分指标的差异可能与梅花鹿生茸、性别等生物学特性有关。对上述生理生化指标的测定,获得了不同性别的梅花鹿在不同生长阶段生理生化指标的参考范围,可为梅花鹿选种育种、疾病诊断、资源开发利用提供一定的参考,也可为野生东北梅花鹿资源保护和健康评估提供依据。  相似文献   

13.
药物对球虫感染鸡血液及生化指标的影响   总被引:9,自引:1,他引:8  
1日龄艾维因肉鸡分为 4个组 ,即中药组、马杜霉素组、感染不给药组和不感染不给药组 ,每组 2 0只。中药组从 1日龄开始即投喂含 0 .1%中草药复方制剂 (由绞股蓝皂甙、去油鸦胆子仁等组成 )的饲料 ;马杜霉素组从 12日龄起投喂含马杜霉素 5 mg/ kg的饲料。前 3组于 14日龄感染鸡柔嫩艾美耳球虫孢子化卵囊。卵囊攻击前及攻击后 4、7d分别采血 ,测定血液红细胞数、血钾、血钠、血磷、血清白蛋白和血清总蛋白含量。结果表明 ,卵囊攻击后 4、7d,感染不给药组的红细胞数显著低于其他各组 (P<0 .0 5 ) ,卵囊攻击后 7d,血钾浓度显著高于其他各组 (P<0 .0 5 ) ,血清白蛋白和血清总蛋白显著低于其他各组 (P<0 .0 5 ) ;卵囊攻击后 7d,中药组血液红细胞数显著低于不感染不给药组 (P<0 .0 5 ) ,但显著高于感染不给药组 (P<0 .0 5 ) ;马杜霉素组的各项测定指标与不感染不给药组相比无显著差异 (P>0 .0 5 ) ;卵囊攻击后 ,各组间的血磷和血糖值无显著差异 (P>0 .0 5 )。结果提示 ,药物能抵抗球虫感染 ,血液各项指标得以保持相对恒定。  相似文献   

14.
This experiment was conducted to investigate the effect of feeding phytoestrogens on milk composition, blood biochemical index and antioxidant capacity of dairy cows.A total of 40 Holstein cows were selected and randomly divided into 4 groups by health, age and lactation stage. The cows in control group was fed total mixed rations(TMR), and the other in 3 trial groups were fed TMR supplemented with 2.5 g dainzein(group Ⅰ), 35 g formononetin (group Ⅱ), 1.25 g dainzein and 17.5 g formononetin (group Ⅲ), respectively. The total duration of the trial was 127 d, the first 7 d of the trial was the preliminary period and the formal trial lasted for 120 d. The milk samples were collected in the trial of 0, 30, 60, 90, 120 d, respectively, and determination of milk composition. The blood samples were collected by jugular vein in the trial, and plasma was separated by 15 min, 3 500 r/min centrifugation, and saved at -20 ℃ for later use. The result showed as follows: Compared with the control group, the milk yield and milk protein yield in groups Ⅰ and Ⅲ were significantly increased (P< 0.05),the lactose yield in groups Ⅰ,Ⅱ and Ⅲ were significantly increased (P< 0.05), the T-AOC activity of blood in group Ⅲ was significantly increased (P< 0.05), the GSH-Px activity of blood in groups Ⅱ and Ⅲ were significantly increased (P< 0.05), the MDA activity of blood in groups Ⅰ and Ⅱ were significantly decreased (P< 0.05), the milk fat yield,lactose rate and TP, ALB, GLB of blood were not significantly increased (P> 0.05),the CAT, SOD activity of blood in trail groups had a upward trent. It was concluded that feeding phytoestrogens could enhance the milk yield, milk protein yield, lactose yield, the body's antioxidant capacity and immune function of Holstein cows.  相似文献   

15.
试验旨在研究日粮中添喂两种植物雌激素及其组合物对奶牛乳成分、血液生化指标和抗氧化性能的影响。选取健康、年龄及泌乳期相近的40头荷斯坦奶牛,随机分为4组:1个对照组、3个试验组,每组10头。对照组饲喂全混合日粮,试验组在饲喂全混合日粮的基础上添加2.5 g大豆黄酮(试验Ⅰ组)、35 g芒柄花素(试验Ⅱ组)、1.25 g大豆黄酮和17.5 g芒柄花素(试验Ⅲ组)。预试期7 d,正试期120 d。分别于试验的第0、30、60、90、120天采集乳样,测定乳成分;颈静脉采集血液样品,3 500 r/min离心15 min,分离血浆,-20 ℃保存备用。结果表明,与对照组相比,试验Ⅰ、Ⅲ组产奶量、乳蛋白产量均显著提高(P< 0.05);试验Ⅰ、Ⅱ、Ⅲ组乳糖产量显著提高(P< 0.05);试验Ⅲ组T-AOC显著提高(P< 0.05);试验Ⅱ、Ⅲ组GSH-Px活性显著提高(P< 0.05);试验Ⅰ、Ⅱ组MDA含量极显著降低(P< 0.05);乳脂产量、乳糖率及TP、ALB、GLB含量均无显著差异(P> 0.05);CAT、SOD活性均有一定程度的提高。由此可见,日粮中添喂两种植物雌激素及其组合物能够在一定程度上提高荷斯坦奶牛的产奶量、乳蛋白含量和乳糖含量,能够提高奶牛机体抗氧化能力,增强免疫功能。  相似文献   

16.
OBJECTIVE: To determine the effects of intravenous (IV) butorphanol on the cardiopulmonary system and on the bispectral index (BIS) in isoflurane-anesthetized alpacas. STUDY DESIGN: Randomized, blinded cross-over experimental trial. ANIMALS: Eight healthy, young (3 +/- 1 SD years) adult female alpacas weighing 64 +/- 9 SD kg. METHODS: Alpacas were anesthetized with isoflurane by mask followed by tracheal intubation and maintenance of anesthesia with isoflurane in oxygen and intermittent positive pressure ventilation. Animals were assigned to two treatments, butorphanol (0.1 mg kg(-1), IV) and saline (0.01 mL kg(-1), IV) in a randomized manner allowing a 2-week interval between treatments. Cardiovascular variables included systolic, diastolic, and mean arterial blood pressure, heart rate, pulmonary arterial pressure, pulmonary arterial occlusion pressure (PAOP), central venous pressure, cardiac output, and pulmonary temperature (TEMP). Cardiac index, systemic vascular resistance (SVR), and pulmonary vascular resistance (PVR) were calculated. Bispectral index was also measured. Arterial and mixed venous blood samples were collected for blood gas analysis. All variables were recorded at baseline (time 0) and at 5, 10, 15, 30, 45 and 60 minutes following injection and were analyzed by using repeated-measures ANOVA (p < 0.05). PAOP, PVR, and BIS were analyzed by paired t-tests. RESULTS: Butorphanol decreased SVR at all times when compared with the baseline, but no difference was detected between treatments. TEMP decreased with time in both treatments, but they were not different from each other. Other cardiovascular, BIS, and blood gas variables were not different between groups. CONCLUSION AND CLINICAL RELEVANCE: We conclude that butorphanol had minimal effects on the cardiovascular system of the alpacas, causing a mild decrease in SVR.  相似文献   

17.
应用间接血凝试验(IHA)对采自杨陵区食品公司屠宰点、西北农业大学实验农场种猪场,西北农林科技大学兽医院的214份猪血样进行弓形虫抗体的定性检测,包括血清检测法(176份),滤纸干血滴法(38份)两种方法。结果检出阳性血样5份,阳性率为2.34%。其中来自杨陵区食品公司屠宰点猪血样136份,全部用血清检测法测定结果,阳性5份,阳性率为3.68%,来自西北农林科技大学农场种猪场血样76份,38份血清  相似文献   

18.
OBJECTIVE: To compare blood glucose (BG) concentrations measured with a portable blood glucose meter in blood samples obtained with a marginal ear vein (MEV) nick technique, from a peripheral venous catheter, and by direct venipuncture in healthy cats and cats with diabetes mellitus. DESIGN: Prospective study. ANIMALS: 1 0 healthy cats and 11 cats with diabetes mellitus. Procedure-On day 1, blood samples were collected every hour for 10 hours by the MEV nick technique and from a peripheral venous catheter. On day 2, blood samples were collected every hour for 10 hours by the MEV nick technique and by direct venipuncture of the medial saphenous vein. RESULTS: For all cats, mean BG concentration for samples collected by the MEV nick technique was not significantly different from mean concentration for samples obtained from the peripheral venous catheter. For healthy cats, mean BG concentration for samples collected by the MEV nick technique was not significantly different from mean concentration for samples obtained by direct venipuncture. For cats with diabetes mellitus, mean BG concentration for samples collected by the MEV nick technique was significantly different from mean concentration for samples obtained by direct venipuncture; however, for the range of concentrations examined, this difference was not clinically important. Conclusions and Clinical Relevance: Results suggest that for the range of concentrations examined, the MEV nick technique is a reasonable alternative to venous blood collection for serial measurement of BG concentrations in cats.  相似文献   

19.
Viral isolation, polymerase chain reaction (PCR), dot blot hybridization (DBH), and indirect enzyme-linked immunosorbent assay (iELISA) were used for the diagnosis of lumpy skin disease in clinically infected, fevered, and apparently normal dairy cows. Lumpy skin disease virus (LSDV) was isolated from skin biopsies and blood samples collected from clinically infected cows in percentages of 72% and 20%, respectively. The virus recovered from blood samples collected from fevered cows in percentage of 33.3%. Both PCR and DBH detected viral DNA in 100% of skin biopsies collected from clinically infected cows whereas the detection rates in blood samples collected from clinically infected animals were 100% and 84% using PCR and DBH, respectively. Viral DNA was detected in blood samples collected from fevered cows using PCR and DBH in percentages of 77.8% and 66.6%, respectively. Only 19.1% of blood samples collected from in-contact cows was positive for both of PCR and DBH. Detection rates of antibodies against LSDV using iELISA in serum samples collected from clinically infected and fevered cows were 56% and 11.1%, respectively, whereas all in-contact cows had no antibodies against the virus.  相似文献   

20.
Blood gas values were compared in blood collected from cut toenails and femoral arteries in 50 healthy crossbred dogs that were sedated and allowed to breathe room air spontaneously. Blood samples from cut toenails were collected by microcapillary technique with Natelson tubes. Femoral artery samples were collected by arterial puncture. Blood values for PO2, PCO2, pH, and HCO3 were compared. There was good correlation for pH, PCO2, and bicarbonate, but not for PO2. Microcapillary samples should be collected in 10 seconds or less for the most accurate results. A metal mixing "flea" was unnecessary. When properly handled, the Natelson tube technique provides an alternative method for collection of blood gas samples.  相似文献   

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