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妊娠相关糖蛋白(pregnancy-associated glycoprotein,PAG)是由胎盘组织产生并释放到母体外周血液中的大分子蛋白,在妊娠过程中发挥着重要作用.目前,母畜妊娠早期诊断的研究多集中在对奶牛伤害小、准确性高、特异性强的间接检测方法上,如检测血液或乳汁中孕酮等激素,而近年来发展起来的对血液中PAG的检测就是一种主流的方法.作者现就PAG的功能、生化特性、母畜怀孕期间PAG的浓度变化及其在反刍动物早期妊娠诊断中的应用情况进行综述. 相似文献
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《中国畜牧杂志》2019,(12)
为探讨荣昌猪母猪在妊娠期不同阶段血清免疫球蛋白G(IgG)的变化情况及其调控机理,本研究选用12头体况、胎次和配种日期一致的妊娠母猪,分别于妊娠第5、35、110天采血并进行屠宰,测定血清IgG及其他血液生化指标,同时测定乳腺、脾脏和淋巴组织中的PI3K-AKT-FOXO1信号通路几个关键基因的表达情况。结果表明:荣昌猪母猪在妊娠第5天(妊娠初期)血清IgG含量最高,淋巴组织的AKT mRNA表达量、脾脏组织的FOXO1 mRNA表达量高于妊娠第35、110天(P<0.05);在妊娠第5天,PI3K在脾脏中的mRNA表达量高于乳腺和淋巴组织(P<0.05)。本研究表明PI3K-AKT-FOXO1信号通路在妊娠初期对IgG的调控作用强于其他妊娠阶段。 相似文献
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夏季高温时节，如果防暑降温工作处理不当，将会给养猪生产造成很严重的损失。
妊娠早期，母猪对高温的耐力很差，当外界温度长时间超过32℃时，妊娠母猪通过血液调节已维持不了自身的热平衡而产生热应激，胚胎死亡率会明显增加。高温能使母猪的子宫内环境发生许多不良改变，使早期妊娠母猪的胚泡附植受阻，胚胎成活率明显降低，产仔数减少，死胎、畸形胎增多。 相似文献
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夏季高温时节,如果防暑降温工作处理不当,将会给养猪生产造成很严重的损失。妊娠早期,母猪对高温的耐力很差,当外界温度长时间超过32℃时,妊娠母猪通过血液调节已维持不了自身的热平衡而产生热应激,胚胎死亡 相似文献
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本试验旨在研究妊娠后期母猪饲粮中添加壳寡糖(COS)对新生仔猪抗氧化能力的影响。选择体重、胎次和预产期一致的健康"长×白"妊娠母猪16头,随机分为2个组,每组8个重复,每个重复1头母猪。对照组饲喂基础饲粮,COS组在基础饲粮中添加30 g/t COS。试验从母猪妊娠85 d开始至产仔结束。结果显示:1)妊娠后期母猪饲粮中添加COS对母猪繁殖性能没有显著的影响(P0.05),但是在一定程度上可以缩短母猪产程。2)妊娠后期母猪饲粮中添加COS显著提高了母猪血液中总超氧化物歧化酶(TSOD)活性(P0.05),并有降低血液中丙二醛(MDA)含量的趋势(P0.05)。3)妊娠后期母猪饲粮中添加COS显著提高了新生仔猪血液中谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性(P0.05),并有降低MDA含量的趋势(P0.05)。4)妊娠后期母猪饲粮中添加COS显著提高了新生仔猪回肠CAT、空肠谷胱甘肽过氧化物酶4(GPX4)的相对表达量(P0.05)。由此可见,妊娠后期母猪饲粮中添加COS缩短了母猪产程,一定程度上能提高母猪及新生仔猪抗氧化能力。 相似文献
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枫泾母猪发情周期和妊娠早期外周血清孕酮含量测定 总被引：4，自引：0，他引：4
枫泾猪是中国太湖地区繁殖力高的地方猪种。本试验测定了8头枫泾和4头长白青年母猪发情周期和妊娠早期外周血清孕酮含量。这两个品种发情周期血清孕酮含量有相似的变化,峰值均出现在发情周期9～12天,枫泾猪为26.58ng/ml,长白猪为25.17ng/ml。在妊娠早期的变化也大致相同。枫泾和长白两种猪在妊娠-3～12天与发情周期-2～12天的孕酮含量变化也差不多,妊娠12天峰值枫泾为21.54ng/ml,长白为28.11ng/ml;在妊娠12天以后两种猪的孕酮含量均略有下降;16～20天后又开始回升,并维持在较高水平。发情周期和妊娠早期外周血清孕酮含量16天后有明显差异(P<0.001),可试用于猪的早期妊娠诊断。据初步测定,枫泾母猪妊娠12及16天孕酮含量与黄体数或胚胎数之间无明显相关(P>0.05)。 相似文献
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E Kanitz B Lampe M Kitzig G Bl?dow F Schneider K Spitschak 《Archiv fuer experimentelle veterinaermedizin》1989,43(3):359-366
Tens gilts received 400 I.U. of Pregmagon on the 11th day of pregnancy (day of KB2 = 1 day of pregnancy), while 8 sows were used as controls. Blood was sampled 4 times a day between the 11th and 25th day of pregnancy, with an indwelling catheter being used. Progesterone, oestradiol-17 beta, and luteinising hormone were radio-immunologically determined. The hormone profiles of pregnant sows were found to differ unambiguously from those of non-pregnant animals for both concentrations and frequencies. They were indicative of close correlations and time links of gonadotrophic and steroid hormones in early pregnancy and in oestrus. PMSG treatment in pregnancy resulted in increase in the oestradiol-17 beta concentration and attenuation in pulsatile luteinising hormone release at the time of implantation. Progesterone concentrations in peripheral blood were not affected by PMSG injection. 相似文献
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《畜牧与生物技术杂志(英文版)》2015,(3)
Background:Early pregnancy failure has a profound impact on both human reproductive health and animal production.2/3 pregnancy failures occur during the peri-implantation period;however,the underlying mechanism(s)remains unclear.Well-organized modification of the endometrium to a receptive state is critical to establish pregnancy.Aberrant endometrial modification during implantation is thought to be largely responsible for early pregnancy loss.Result:In this study,using well-managed recipient ewes that received embryo transfer as model,we compared the endometrial proteome between pregnant and non-pregnant ewes during implantation period.After embryo transfer,recipients were assigned as pregnant or non-pregnant ewes according to the presence or absence of an elongated conceptus at Day 17 of pregnancy.By comparing the endometrial proteomic profiles between pregnant and non-pregnant ewes,we identified 94 and 257 differentially expressed proteins(DEPs) in the endometrial caruncular and intercaruncular areas,respectively.Functional analysis showed that the DEPs were mainly associated with immune response,nutrient transport and utilization,as well as proteasome-mediated proteolysis.Conclusion:These analysis imply that dysfunction of these biological processes or pathways of DEP in the endometrium is highly associated with early pregnancy loss.In addition,many proteins that are essential for the establishment of pregnancy showed dysregulation in the endometrium of non-pregnant ewes.These proteins,as potential candidates,may contribute to early pregnancy loss. 相似文献
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Oshima K Yoshihara K Kojima T Komatsu M Yamamoto N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):799-805
Macrophage colony-stimulating factor (M-CSF) is a hemopoietic cytokine with a primary role in placental physiology. Gene expression of M-CSF in the bovine endometrium shows a temporal upward trend during early and mid pregnancy. This study determined the plasma M-CSF levels during pregnancy using ELISA. In experiment 1, to investigate the relationship between the concentration of M-CSF in peripheral blood and pregnancy, the plasma M-CSF levels were determined in 125 pregnant and 21 non-pregnant Japanese Black cows. The pregnant animals were divided into nine groups based on the month of pregnancy. An ELISA for bovine M-CSF established previously was used according to the authors' instructions. In experiment 2, the plasma M-CSF level was determined to investigate the temporal changes in its concentration in the peripheral blood during pregnancy. In experiment 1, the plasma M-CSF level varied from month to month during pregnancy; the mean level in the first-month of pregnancy was significantly higher than those in the third and last months of pregnancy and non-pregnancy (P<0.05). In experiment 2, the plasma M-CSF level varied with the day of pregnancy (P<0.05). The mean level of plasma M-CSF decreased gradually until 6 weeks of pregnancy; it appeared to increase during weeks 7-9, then varied with several small peaks until 27 weeks of pregnancy and finally decreased gradually until parturition. These results suggest that the plasma M-CSF level may be related to changes in the uterus and placenta as pregnancy progresses. 相似文献
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旨在筛选人工授精后第17天奶牛在不同妊娠状态时的候选生物标志物。本研究以宁夏某奶牛场体重为（550±50） kg,体况评分相近的经产（2~3胎次）健康荷斯坦奶牛为试验对象。同期发情后,在人工授精后第17天晨饲前对奶牛进行尾静脉采血,后期采用计步器和B超仪诊断出奶牛的妊娠状态。根据诊断结果将奶牛分为妊娠组（A组,n=12）和未妊娠返情组（B组,n=24）,将这两组血样进行代谢轮廓及代谢物变化分析。主成分分析（PCA）和正交偏最小二乘法-判别分析（OPLS-DA）结果显示,A、B两组血浆代谢轮廓均发生了明显变化,A、B两组之间共检测出8种ROC （Receiver operating characteristic curve）曲线下面积（area under the ROC curve,AUC）＞0.8的差异代谢物。丙氨酸-亮氨酸、甘氨酸、脯氨酸、L-正亮氨酸、DL-苯丙氨酸、肌氨酸、吡咯-2-羧酸和缬氨酸-蛋氨酸有望成为识别妊娠组（A组）和未妊娠返情组（B组）的差异代谢物。综上表明,血浆中的这8种代谢物有望成为奶牛妊娠识别阶段潜在的生物标志物,为配种早期妊娠识别提供新思路。 相似文献
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Haichao Zhao Linlin Sui Kai Miao Lei An Dong Wang Zhuocheng Hou Rui Wang Min Guo Zhilong Wang Jiqiang Xu Zhonghong Wu Jianhui Tian 《畜牧与生物技术杂志(英文版)》2015,6(1)
Background
Early pregnancy failure has a profound impact on both human reproductive health and animal production. 2/3 pregnancy failures occur during the peri-implantation period; however, the underlying mechanism(s) remains unclear. Well-organized modification of the endometrium to a receptive state is critical to establish pregnancy. Aberrant endometrial modification during implantation is thought to be largely responsible for early pregnancy loss.Result
In this study, using well-managed recipient ewes that received embryo transfer as model, we compared the endometrial proteome between pregnant and non-pregnant ewes during implantation period. After embryo transfer, recipients were assigned as pregnant or non-pregnant ewes according to the presence or absence of an elongated conceptus at Day 17 of pregnancy. By comparing the endometrial proteomic profiles between pregnant and non-pregnant ewes, we identified 94 and 257 differentially expressed proteins (DEPs) in the endometrial caruncular and intercaruncular areas, respectively. Functional analysis showed that the DEPs were mainly associated with immune response, nutrient transport and utilization, as well as proteasome-mediated proteolysis.Conclusion
These analysis imply that dysfunction of these biological processes or pathways of DEP in the endometrium is highly associated with early pregnancy loss. In addition, many proteins that are essential for the establishment of pregnancy showed dysregulation in the endometrium of non-pregnant ewes. These proteins, as potential candidates, may contribute to early pregnancy loss.Electronic supplementary material
The online version of this article (doi:10.1186/s40104-015-0017-0) contains supplementary material, which is available to authorized users. 相似文献16.
The pregnancy diagnosis in sows using direct radioimmunoassay of estrone-sulphate in the blood serum without sample extraction is described. It was found that for the pregnancy diagnosis the period between days 22 and 30 of pregnancy can be used since in this period the estrone-sulphate concentrations in all pregnant sows markedly exceeded those of 64 non-pregnant animals. The estrone-sulphate estimation cannot be used for the pregnancy diagnosis before day 22 and between day 30 and 40 because the estrone-sulphate concentrations in most samples collected in these periods were lower than 4 nmol.l-1. 相似文献
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In four Kenyan pig breeding units the pregnancy diagnosis of sows has been carried out in two groups: Group 1 (n = 1911): the sows were transrectaly pregnancy tested between Days 17-22 post-mating by ultrasound. Sows testing non-pregnant immediately received one dose of 400 IU pregnant mare serum gonadotropin (PMSG) (equine chorion gonadotropin, eCG) and 200 IU human chorion gonadotropin (hCG). On showing signs of oestrous, the animals were subsequently artificially inseminated (AI). Group 2 (n = 1923): sows were pregnancy tested by serum progesterone (P4)-based enzyme-linked immunosorbent assay (ELISA) on Day 17 post-breeding. P4 concentrations were categorized as positive (> 5 ng/ml) or negative (< 5 ng/ml). Sows testing nonpregnant immediately received one dose of 400 IU PMSG and 200 IU hCG by injection, and were subsequently artificially inseminated. The following parameters were evaluated: sows diagnosed non-pregnant, days from first post-weaning insemination until the sows were inseminated at their first return to oestrus; farrowing rate and total piglets born and number of live-born piglets in litters. The percentage of sows diagnosed non-pregnant in the two groups, as well as the totals of born piglets and of live-born piglets in litters did not differ significantly between the two groups. The number of days from the first post-weaning mating until the sows were artificially inseminated at their first return to oestrus and the administration of eCG and hCG was shorter (P < 0.01) and farrowing rate was higher (P< 0.01) in the ELISA-tested sows. 相似文献
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Blood samples taken from the ear vein of 1037 sows two to three weeks after service were assayed the same day using Ovucheck 'Sowside' kits. Colour development was compared with oestrous and pregnancy controls. Reliable data on reproductive performance were obtained from 908 sows sampled 17 to 20 days after service. The accuracy of identification of 796 pregnant and 12 non-pregnant sows was 94.6 per cent and 35.7 per cent, respectively. Excluding animals which returned outside the normal range of 18 to 24 days, 52.1 per cent of 48 empty sows were identified by the test. Problems with blood sampling were reported on seven of 18 farms and this may explain the low accuracy of the kit on some farms. 相似文献
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