首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 31 毫秒
1.
Aerobic bacteria in pond water, sediments, and Artemia in earthen saline ponds in Saudi Arabia were quantified. Total viable counts (TVC; mean ± SD) in pond water ranged between 7.9 ± 4.8 × 103 and 1.6 ± 3.7 × 104 colony forming units (cfu)/mL; in sediments between 7.1 ± 4.2 × 106 and 6.5 ± 3.8 × 107 cfu/g; on Artemia surfaces, 3.9 ± 0.9 × 103 and 1.0 ± 2.9 × 104 cfu/Artemia; and in Artemia homogenate, 4.6 ± 2.4 × 107 and 3.2 ± 3.6 × 108 cfu/g. The bacterial flora was predominantly Gram-negative rods, accounting for 89% of total isolates. Altogether, nine bacterial species of seven genera were identified. Bacteria in pond sediments were the most diverse compared to other populations. In all populations, Vibrio alginolyticus, Vibrio sp., and Staphylococcus sp. were dominant (P < 0.005). The bacterial flora of pond water and sediments were reflected in the bacterial composition on and in Artemia.  相似文献   

2.
ABSTRACT

Quantitative and qualitative estimation of bacterial flora present in pond water, sediments, gills, and intestine of healthy common carp Cyprinus carpio cultured in Saudi Arabia were performed and identified to species level where possible. Mean total viable bacterial counts in pond water ranged from 1.2?±?2.9?×?104 to 2.5?±?3.5?×?105 cfu/mL; in sediments, 9.3?±?2.1?×?107 to 2.7?±?3.5?×?109 cfu/g; in gills filaments, 4.3?±?2.9?×?106 to 1.6?±?3.9?×?107 cfu/g; and in intestine, 8.7?±?4.1?×?109 to 5.4?±?3.2?×?1010 cfu/g. Gram-negative rod-shaped bacteria dominated (76%) the populations. In total, 12 bacterial genera and 15 species were identified. Pond water and sediment bacteria had the reflection on bacterial composition of gills and intestine of carp. Intestinal bacteria showed more diversification in contrast to gill bacteria. Aeromonas hydrophila, Shewanella putrefaciens, Vibrio cholerae, Corynebacterium urealyticum, Vibrio vulnificus, Vibrio sp., and Staphylococcus sp. were the common bacteria in all the populations. In pond water and carp intestine, A. hydrophila, S. putrefaciens, V. cholerae, and C. urealyticum were the most dominant bacteria (prevalence ≥ 10%) where pond sediments and the carp gills experienced with more one dominant bacterium V. vulnificus. Only the A. hydrophila covered one fourth (25%) of the total bacterial populations.  相似文献   

3.
Quantitative and qualitative analyses of bacterial flora associated with pond water, gills, and intestine of polycultured healthy common carp (Cyprinus carpio) and African catfish (Clarias gariepinus) were carried out and identified to species level where possible. Total viable bacterial counts in the pond water ranged from 9.2?±?5.5?×?103 to 6.6?±?5.1?×?104 colony-forming units (cfu)/mL; in the gill filaments of carp and catfish, 3.3?±?3.8?×?106 to 7.9?±?5.6?×?106 and 1.1?±?4.6?×?105 to 2.3?±?5.2?×?106 cfu/g, respectively; and in the intestine of carp and catfish, 1.4?±?2.9?×?1010 to 1.7?±?6.0?×?1011 and 2.7?±?3.4?×?1010 to 1.0?±?4.5?×?1011 cfu/g, respectively. Gram-negative rod-shaped bacteria dominated the populations: 90% in carp, 89% in catfish, 80% in water, and 86% in the total populations. Altogether, 14 bacterial species of 10 genera were identified in total populations. Pond water bacteria had a reflection on the bacterial composition of the gills and intestine of carp and catfish. Aeromonas hydrophila, Shewanella putrefaciens, Vibrio cholerae, Staphylococcus sp., and Vibrio vulnificus appeared as the common bacteria in the populations, where the first three were highly significantly abundant (P?<?0.0001). Moreover, A. hydrophila was the most significantly dominant bacteria (32%; P?<?0.005) among the total populations. Pantoea sp. and Pasteurella pneumotropica were present only in carp and catfish, respectively, but Corynebacterium urealyticum and Micrococcus sp. were present only in pond water.  相似文献   

4.
The bacterial flora of the rearing pond water and sediment as well as the gills and intestine of healthy hybrid tilapia cultured in Saudi Arabia was estimated quantitatively and qualitatively, the isolates being identified at genus or species level. Total viable counts of bacteria (measured as colony‐forming units, cfu) were in the range 5.6 ± 0.8 × 103 to 2.4 ± 1.2 × 104 cfu mL?1 in pond water; 9.3 ± 1.1 × 106 to 1.9 ± 1.5 × 108 cfu g?1 in sediment; 7.1 ± 0.7 × 105 to 8.7 ± 1.1 × 106 cfu g?1 in the gills of tilapia; and 3.4 ± 1.8 × 106 to 5.8 ± 0.4 × 107 cfu g?1 in the intestine of tilapia. In total, 15 bacterial genera and 18 species were identified. Pond water and sediment bacteria reflected the bacterial composition in the gills and intestine of tilapia. In contrast to gill bacteria, more diversification was observed in intestinal bacteria. Corynebacterium urealyticum, Shewanella putrefaciens and Aeromonas hydrophila predominated in all samples. In pond water, C. urealyticurn, S. putrefaciens, A. hydrophila, Flavobacterium sp. and Pseudomonas sp. were the most predominant bacterial species (prevalence > 10%), whereas A. hydrophila, C. urealyticum, S. putrefaciens and Escherichia coli were predominant in pond sediment, and C. urealyticum, S. putrefaciens and A. hydrophila were predominant in both the gills and intestine of tilapia.  相似文献   

5.
Total bacterial load, total coliforms faecal coliforms in pond water, sediment, intestine of hybrid tilapia Oreochromis niloticus×Oreochromis aureus and pigeon Columba livia faeces were investigated monthly over a period of 1 year from July 1999 to June 2000. Fish were collected randomly by a cast net. Samples were analysed for coliforms using the multiple‐tube fermentation technique. Results showed total viable bacterial counts in the pond water, sediment, intestine of tilapia and pigeon faeces ranging from 1.8±0.9×102 to 6.0±1.2×104 cfu mL?1, 3.2±1.2×105 to 2.8±1.5×107 cfu g?1, 8.2±1.6×105 to 9.9±1.5×107 cfu g?11.0±0.4×107to9.7±0.2×109 cfu g?1respectively. The most probable number (MPN) of coliforms and faecal coliforms ranged from 287±12 to ≥1600±0 100 mL?1 in pond water; the MPN ranges for sediment, tilapia intestine and pigeon faeces were 257±29 to ≥1100±0 g?1, 237±46 to ≥1100±0 g?1 and 403±98 to ≥1100±0 g?1 respectively. The abundance of normal bacteria coliforms was greater in the warm months than in the cold months. Ground water was free from any sort of coliform organisms, and there were no sources of human faecal matter in the pond. So, it is clear that faecal coliforms from pigeon faeces significantly contaminated (P<0.05) the ponds and tilapia intestines. Escherichia coli was the only coliform organism found in pond water, sediment, intestine of tilapia and pigeon faeces.  相似文献   

6.
The antibacterial effect of oxytetracycline (OTC) on bacterial load, pathogen and possible development of antibiotic resistance in intestinal flora of apparently healthy Nile tilapia (Oreochromis niloticus) was investigated under laboratory condition. OTC, a broad spectrum approved antibiotic widely used in aquaculture, was fed to Nile tilapia through medicated diet at a rate of 2 g/ kg of feed for consecutive 7 days in treatment 1 (T1) and 30 days in treatment 2 (T2). Changes in physico‐chemical parameters of water were also recorded where pH and dissolved oxygen influenced bacterial load and coliform count respectively. At the start of the experiment, initial bacterial load in fish intestine was 1.61 ± 2.25 × 1010 cfu/g, which after OTC treatment decreased significantly (p < .05) to 3.06 ± 2.08 × 108 cfu/g on day 7 and 3.45 ± 4.46 × 107 cfu/g on day 30 when compared with the control group. Among the 10 bacterial genera identified in the intestine, 4 potential public health concern bacteria viz., Salmonella, Escherichia, Enterobacter and Staphylococcaus were predominant. There was a sharp increase in bacterial load on day 18 in T2 that may be attributed to the development of antibiotic resistance in intestinal bacteria. Antibiotic susceptibility test for isolates against seven antibiotics: amoxycillin, ciprofloxacin, co‐trimoxazole, erythromycin, gentamicin, nitrofurantoin and tetracycline using disc diffusion method revealed significantly increased resistance of Gram‐negative rods (p < .05) that possibly caused increased frequency of OTC‐resistant microorganisms. In conclusion, short‐ and long‐term exposure to OTC treatment affected the distribution of bacterial genera including pathogens in the Nile tilapia gastrointestinal tract and concomitantly influenced their antimicrobial resistance.  相似文献   

7.
高产鱼池中异养细菌的初步研究   总被引：16，自引：4，他引：16  
对无锡市效区“河埒渔业一队”典型高产鱼池中异养细菌种类、数量及群落组成的分析测定,它表明在1984年3月至10月试验期间,试验鱼池水体中总细菌数和异养细菌数分别在1.30×10~5～15.90×10~5个/ml和1.56×10~4～14.50×10~4个/ml。鱼池淤泥中总细菌数和异常细菌数分别在1.20×10~6～68.0×10~5个/克和1.94×10~5～19.90×10~5个/克。同时经分析得知,试验鱼池中细菌数量的波动与鱼池水温等理化因子各有不同的相关关系。试验鱼池水体中共分离出11个属的异养细菌,其中优势菌为氮单胞菌属(Azomonas)等具固氮能力的菌属;淤泥中共分离出8个属的异养细菌,其中优势菌为芽孢杆菌属(Bacillus)等革兰氏阳性菌属。假单胞菌属(Pseudomonas)的细菌普遍存在于鱼池的水体及淤泥中。试验表明,高产鱼池中的细菌数量变化有一定规律性,细菌数量及异养细菌的群落组成与鱼池的鱼产量有一定的关系。  相似文献   

8.
This study examined the diversity of siderophore‐producing bacteria in the intestinal tracts of coastal fish in Japan and screened candidate bacterial strains for probiotic use in aquaculture. Of the 2637 bacteria isolated from the 27 fish specimens (13 species) and six environmental samples collected in this study, 266 isolates exhibited the ability to produce siderophores. Siderophore producers were detected in the intestines of 18 of the fish specimens caught (68%) at densities of 2.3 × 104–2.3 × 108 CFU g?1, in all three seawater samples at 2.0 × 102–1.3 × 103 CFU mL?1 and in all three sand samples at 2.6 × 101–2.8 × 104 CFU g?1. These findings suggest that siderophore‐producing bacteria are widely distributed in the intestinal tracts of coastal fish and their environments. Analysis of 16S rRNA gene sequences revealed that the siderophore producers belonged to 38 species, of which Vibrio splendidus, Vibrio ichthyoenteri and Vibrio crassostreae accounted for 32.7%, 19.5% and 11.3% of the 266 isolates, respectively, suggesting that these bacteria are indigenous to the intestinal tract of coastal fish. Six bacterial species, Enterovibri norvegicus, Photobacterium leiognathi, Photobacterium phosphoreum, Photobacterium rosenbergii, V. crassostrea and Vibrio scophthalmi were identified as possible candidates for use as probionts in fish aquaculture.  相似文献   

9.
为探讨一株杀鱼假交替单胞菌 2515 Pseudoalteromonas piscicida (简称菌株 2515)在对虾养殖中的抗弧菌效果, 以鳗弧菌(Vibrio anguillarum)为指示菌, 以鳗弧菌的敏感抗生素新霉素为参比, 采用牛津杯打孔法, 比较了菌株 2515 与抗生素抗菌等效关系。设置浓度分别为 104 CFU/mL、105 CFU/mL、106 CFU/mL、107 CFU/mL 的菌株 2515 与鳗弧菌共培养, 评价了该菌对鳗弧菌的拮抗效果。将含量 103 CFU/g、105 CFU/g、107 CFU/g, 及 103 CFU/mL、 105 CFU/mL、107 CFU/mL 的菌株 2515 分别添加到对虾饲料和养殖水体中, 养殖凡纳滨对虾(Litopenaeus vannamei), 分析了菌株 2515 对水体和对虾肠道细菌总数、弧菌数及对抗副溶血弧菌效果的影响。结果显示, 相同抗鳗弧菌效果条件下菌株 2515 数量与新霉素质量存在对应关系, 与鳗弧菌混合培养时, 浓度 106 CFU/mL 及 107 CFU/mL 的菌株 2515 对鳗弧菌表现出强的抑菌作用。饲料中添加菌株 2515 浓度 105 CFU/g 及 107 CFU/g 时能够显著降低对虾肠道中的弧菌数量(P < 0.05), 提高对虾养殖的存活率。采用副溶血弧菌(V. parahaemolyticus)攻毒, 显示在饲料中添加菌株 2515 浓度为 105 CFU/g 一组对虾死亡率最低, 为(43.3±5.8)%, 对副溶血弧菌的相对保护率达(53.7±6.2)%。结果表明, 在饲料和养殖水体中添加适宜浓度的菌株 2515 均能够提高凡纳滨对虾抗副溶血弧菌的能力, 降低对虾肠道内的弧菌数量。本研究结果为对虾养殖弧菌病生物防控提供了一种技术支撑。  相似文献   

10.
养殖大黄鱼加工和冰藏过程中鲜度和细菌类型的变化   总被引：7，自引：5，他引：2  
通过对养殖海水以及捕获、加工、流通和冰藏等整个供应链过程养殖大黄鱼的温度履历、感官、化学、微生物的品质监测,评估养殖海水、碎冰和加工过程中大黄鱼细菌卫生状况,分析其在整个过程鲜度和细菌相变化。结果表明,养殖海水和碎冰的菌落总数分别为4.99±0.41 lg cfu/g和3.90±0.32 lg cfu/g,大肠菌群数分别为30~450 MNP/100 g和低于30 MNP/100 g;新捕获养殖大黄鱼的菌落总数为3.82±0.38 lg cfu/g,假单胞菌数为3.13±0.58 lg cfu/g,嗜冷菌数为2.82±0.60 lg cfu/g,大肠菌群数为低于30 MPN/100 g;冰藏第18天时TVBN含量为28.62±0.51 mg/100 g,菌落总数、假单胞菌数、嗜冷菌数和大肠菌群数分别为6.24±0.06lg cfu/g、6.01±0.25 lg cfu/g、5.68±0.21 lg cfu/g和30 MPN/100 g。养殖海水和新捕获鱼中细菌种类繁多,革兰氏阴性菌分别占其总菌株数的64.9%和56.8%;革兰氏阳性菌分别占其总菌株数的27.0%和42.0%;优势菌群为黏性威克斯菌、腐败希瓦氏菌和假单胞菌属。加工结束后,假单胞菌(34.9%)、玫瑰小球菌(32.6%)和缺陷短波单胞菌(14.0%)成为主要菌群。冰藏第4天时仅分离出4种不同类型的细菌,腐败希瓦氏菌比例上升明显,冰藏第10天和第18天时,所占比例分别为63.5%和69.1%,是冷却链养殖大黄鱼产品的优势腐败菌。  相似文献   

11.
The study was conducted in nine 150‐L capacity glass aquaria for 192 h to determine the changes in the microflora in the gut and hepatopancreas of tiger shrimp, Penaeus monodon, after bioaugmenting (water treatment and probiotics). Results showed that luminous bacterial counts were significantly lower in bioaugmented than in nonbioaugmented systems (range 0–5.9 × 103 cfu/g and 0–3.2 × 103 cfu/g in gut and hepatopancreas, respectively). Biochemical tests of isolates showed that the bioaugmented systems were dominated by Gram‐positive Bacillus and Streptococcus and the Gram‐negative Vibrio dominated the control. In water‐treated and probiotics‐fed bioaugmented system, the change in bacterial dominance to Bacillus became evident 2 h in the gut and 24 h in the hepatopancreas. Bacterial dominance shifted to Vibrio species after 120 h. Shrimp in the control were observed to become lethargic after 72 h as Vibrio species significantly increased in number. Dissolved oxygen and unionized ammonia levels were significantly lower in bioaugmented system compared with the control.  相似文献   

12.
The effect of Streptomyces strains RL8 and N7 on the growth, survival and histological structure of the hepatopancreas of Litopenaeus vannamei was determined. Shrimp post‐larvae of 0.02 ± 0.01 g were fed with diets supplemented with Streptomyces spp. RL8 and N7 at 108 CFU g?1 of feed for 30 days, followed by challenge with Vibrio parahaemolyticus. Toxicity was also assessed by recording the mortality associated with the use of 1‐100 g/L of cell mass and 108‐1010 CFU/g of feed of these strains in Artemia salina and L. vannamei, respectively. The group treated with Streptomyces sp. RL8 had significantly better weight gain (0.62 ± 0.18 g) and survival rates (84.44 ± 5.7%) after challenge, whereas the one fed with Streptomyces sp. N7 showed significantly higher survival rate (57.77 ± 6.8%) than the control group (0.55 ± 0.20 g and 11.11 ± 9.0%, respectively). The hepatopancreas of Streptomyces‐fed shrimps showed better histological integrity with significantly lower degree of atrophy and necrotic tubules than their unfed counterpart. No toxicity associated with these strains was found. Consequently, they can be excellent probiotic candidates to improve the physiological status and prevent infectious diseases of cultured L. vannamei.  相似文献   

13.
The indiscriminate use of antibiotics and chemicals in shrimp hatcheries has led to biomagnification and that in turn could lead to rejection of a whole consignment. The application of the bioencapsulation technique as a tool for curative treatment in shrimp larvae was investigated. Herbs having antibacterial properties such as Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia (methanolic extracts) were bioencapsulated in Artemia and fed to Penaeus monodon post larvae PL 1–25. The post larvae were reared in a medium inoculated with pathogenic bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Vibrio sp. Post larvae reared in the non-inoculated water and fed with non-enriched Artemia exhibited 90% survival, highest specific growth rate (12.43%) and reduced bacterial load. P. monodon reared in the bacterial inoculated water and fed with the non-enriched Artemia exhibited the lowest survival (10–30%), specific growth rate (8.42–9.1%) and increased bacterial load (2.86 × 103 to 3.76 × 105 cfu/g). The methanolic extracts of the herbs helped to increase survival and specific growth rate and reduced bacterial load in the P. monodon culture system. Among the three herbal extracts, P. corylifolia enriched Artemia fed post larvae showed the tendency to higher survival (>50%), growth rate (11.5 averaged) and low bacterial load (1.12 × 105 cfu/g). This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

14.
The objective of this study was to understand the microbial flora associated with the hatchery system of giant fresh water prawn, Macrobrachium rosenbergii during an entire rearing cycle. Bacteriological and physico-chemical analysis was done for different samples of water, larvae, and Artemia. The total bacterial load in well water, seawater and inlet water varied from 101 to 105 cfu ml− 1 with higher counts seen in larval rearing tank (LRT) water. The Vibrio count ranged between 101 to 103 cfu ml− 1. Larval samples harboured a bacterial load of 106 to 107 cfu/10 larvae. The bacterial load in Artemia hatching water ranged from 4.90 × 104 to 5.63 × 106 cfu ml− 1 while Artemia had a load ranging from 1.08 × 107 to 2.09 × 109 cfu g− 1. Vibrio count in the LRT water ranged from 101-103 cfu ml− 1 while the count in larvae ranged from 102 to 104 cfu/10 larvae. The bacterial genera were predominantly Gram-negative and comprised of Aeromonas spp., Pseudomonas spp., Vibrio spp. and Bacillus spp. and non-spore formers (NSF) were the dominant Gram-positive bacteria. This study documents the bacterial flora associated with Macrobrachium hatchery system during a regular normal run. Knowledge of the qualitative and quantitative aspects of bacterial flora in the hatchery would help to understand disturbances, if any, brought about during disease outbreaks.  相似文献   

15.
The study investigated effect of dietary supplementation with malic acid, Bacillus subtilis and a mixture of the two (3 × 2 factorial trial) on Nile tilapia (Oreochromis niloticus) health. Treatment groups (T1–T6) were fed diets containing three levels of malic acid (0.0, 5.0 and 10.0 g/kg), each of which was supplemented with 0 and 1.1 × 105 cfu/g B. subtilis, respectively. Each treatment group was assigned randomly to triplicate groups of 35 fish (5.26 ± 0.06 g) in 18 tanks for 84 days. The results indicated that survival was higher in all dietary treatments as compared to the control fed fish. The highest values of FBW, WG and SGR (%), PER, PPV and ER were recorded in groups T4 and T6, which were fed diets supplemented with 5 g malic acid/kg and 1.1 × 105 cfu/g B. subtilis and 10 g malic acid/kg and 1.1 × 105 cfu/g B. subtilis, respectively. The best value of FCR was obtained in groups T4 and T6. The lowest total bacterial count in the gut and faeces was detected in fish from group T6. The values of haematocrit, haemoglobin, red blood cells, white blood cells, total protein, albumin and globulin were significantly higher (p < .05) in fish from groups T4 and T6 (diets supplemented with malic acid and B. subtilis). As compared to the control fed fish, te mixture of these substances are promising as immune enhancher in aquacultured fish.  相似文献   

16.
Flavobacterium psychrophilum, the causative agent of bacterial cold‐water disease (BCWD) in freshwater‐reared salmonids, is also a common commensal organism of healthy fish. The virulence potential of F. psychrophilum isolates obtained from BCWD cases in Ontario between 1994 and 2009 was evaluated. In preliminary infection trials of rainbow trout juveniles, significant differences (0% to 63% mortality) in the virulence of the 22 isolates tested were noted following intraperitoneal injection with 108 cfu/fish. A highly virulent strain, FPG 101, was selected for further study. When fish were injected intraperitoneally with a 106, 107 or 108 cfu/fish of F. psychrophilum FPG 101, the 108 cfu/fish dose produced significantly greater mortality (p < 0.05). The bacterial load in spleen samples collected from fish every 3 days after infection was determined using rpoC quantitative polymerase chain reaction amplification and by plate counting. Bacterial culture and rpoC qPCR were highly correlated (R2 = 0.92); however, culture was more sensitive than the qPCR assay for the detection of F. psychrophilum in spleen tissue. Ninety‐seven per cent of the asymptomatic and the morbid fish had splenic bacterial loads of <2.8 log10 gene/copies and >3.0 log10 gene copies/reaction, respectively, following infection with 108 cfu/fish.  相似文献   

17.
In the present study a multiplex real-time PCR method was developed for early detection of diseased fish infected by Aeromonas salmonicida, Vibrio anguillarum, and/or Tenacibaculum maritimum. The method consisted of the detection of three species-specific genes after DNA extraction with a commercial kit. Three types of samples were tested, and the results were compared with those of traditional diagnosis. The method obtained a limit of detection of 104 cfu/mL (2 x 102 cfu/tube). Additionally, 27 samples from fish showing signs of disease were correctly diagnosed by the developed methodology, demonstrating its suitability for implementation in aquaculture.  相似文献   

18.
There are few reports in the literature about the isolation of bacteria from whale intestine. In this report, we counted colony-forming units in the feces obtained from three female common minke whales (Balaenoptera acutorostrata). The number of colony-forming units ranged from (2.2 ± 0.4) × 105 to (8.9 ± 2.0) × 108 per gram (wet weight) of excrement. 16S rRNA gene sequences of 141 isolates were determined. These strains were identified as Enterococcus faecalis, Enterococcus sp., Enterobacter cloacae, Enterobacter sp., Escherichia coli, Edwardsiella ictaluri or Clostridium sp. The data suggested that the facultative anaerobic population of the intestinal bacterial flora of the minke whale was similar to that of ground mammals.  相似文献   

19.
Major fish bacterial diseases in Korea are edwardsiellosis, streptococcosis, and vibriosis. Among vibrionaceae, Listonella anguillarum, Vibrio harveyi, V. ichthyoenteri, and Photobacterium damselae were identified as causative organisms of vibriosis in flounder. In this study, we developed a multiplex PCR method using the RNA polymerase β subunit (rpoB) gene, known as a housekeeping gene for identification of Vibrio spp. causing vibriosis in flounder. Three pairs of PCR primers were designed based on the rpoB sequence of three species, V. harveyi, V. ichthyoenteri, and P. damselae. The PCR assay, using a mixture of six primers, yielded amplicons of 601, 434, and 533 bp in V. harveyi, V. ichthyoenteri, and P. damselae. None of the untargeted species yielded an amplicon. The detection limits for pure culture in kidney were 2.5 × 104 cfu/g kidney for V. harveyi, 2.5 × 105 cfu/g kidney for V. ichthyoenteri, and 2.5 × 106 cfu/g kidney for P. damselae. From the colonies on TCBS agar plates of different samples, 632 Vibrio spp. isolated from aquacultured flounder between 2004 and 2010 were identified by the multiplex PCR method. As a result, 265 strains (41.9 %) were V. ichthyoenteri; 115 strains (18.2 %) were V. harveyi and 72 strains (11.4 %) were P. damselae.  相似文献   

20.
The bacterial flora occurring in brackish pond water, sediment, gills and intestine of healthy tilapia cultured in Saudi Arabia were estimated both quantitatively and qualitatively, and the isolates were identified to genus or species level. Total viable count of bacteria ranged from 1.4±1.5×103 to 8.6±2.7×103 cfu ml−1; 1.2±3.1×106 to 7.3±1.1×107 cfu g−1; 8.7±1.9×105 to 2.1±0.9×106 cfu g−1; and 2.8±2.4×107 to 1.0±1.6×108 cfu g−1 in the pond water, sediment, gills and intestine of brackish water tilapia, respectively. In total, 19 bacterial species were identified. The bacteria were predominantly Gram-negative rods (87%). Pond water and sediment bacteria influenced the bacterial composition of gills and intestine of tilapia. In contrast to gill bacteria, more diversification was observed in intestinal bacteria. The predominant (prevalence >10%) bacterial species were Vibrio parahaemolyticus, Vibrio carchariae, Vibrio alginolyticus, Chryseomonas sp., Vibrio vulnificus, and Streptococcus sp. in all the populations with the exception of the sediment population where Streptococcus sp. was replaced by Shewanella putrefaciens. Vibrio spp. (58% of the total isolates) dominated the total bacterial population.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号