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近年,我区培育柑桔无病苗木的工作正方兴未艾,柑桔黄龙病的危害已在部分地区得到了一定程度的控制。而柑桔裂皮病和柑桔黄龙病一样,也是培育无病苗木时应予以脱除的病毒类病害之一。但是,柑桔裂皮病在我区大多数无病苗圃中迄今仍未能列入工作计划之中。究其原因,一是在我国,对该病的认识和开展研究的时间尚短,只是到了80年 相似文献
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柑桔裂皮病(Exocortis)是由一种类病毒引起的危险性病害。目前在我国的四川、湖南、广东、广西、福建、台湾等省已有发生。1977年在浙江海宁从阿尔巴尼亚引入的甜橙上发现过裂皮病。温州是浙江的重要柑桔产区之一,60年代以来通过各种渠道从四川、湖南等地引入不少新品种。为了保护柑桔生产,免受裂皮病危害,以及防止该病害的传播,我们在近两年对温州主要柑桔产区进行田间调查和鉴定,现将初步调查和鉴定结果报道如下。 相似文献
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大果山楂裂皮病的发生与防治 总被引：3，自引：0，他引：3
调查天峨县大果山楂裂皮病发生危害情况,其发病程度与果树的繁殖方式、品种、树势和管理水平有关、每年８月至翌年元月是发病盛期,其防治的根本措施是培育无病苗木、选用抗病品种和消毒操作机具。 相似文献
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旱柳病虫为害及其防治 总被引：1，自引：0，他引：1
笔者介绍旱柳主要病虫种类及其防治方法,供参考。1杨树烂皮病1.1症状杨树烂皮病又叫腐烂病,主要为害柳树干部和枝梢,幼树发病最重。当造林技术不当或林木受干旱、霜冻、火灾或其他伤害时,病害最易流行。发病初期,树干上出现水渍状病斑,有酒糟味,树皮逐渐腐烂,干缩下陷,病斑扩展很快,当环 相似文献
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柑橘衰退病毒(Citrus tristeza virus,CTV),柑橘碎叶病毒(Citrus tatter\|leaf virus,CTLV),柑橘裂皮病类病毒(Citrus exocortis viroid,CEVd)和柑橘黄龙病(Huanglongbing, HLB)亚洲种病原(Candidatus liberobacter asiaticus)是重要的柑橘嫁接传播病原。本文建立了同时检测HLB病菌、CTV、CEVd 和CTLV 4种柑橘嫁接病原的一步法、双温多重PCR检测技术体系,同时在体系中设置内参基因。应用该体系快速评价了4种嫁接传播病原在田间侵染情况,结果表明28个田间样品CTV、CEVd、CTLV和HLB感染率分别为89.3 %、17.9 %、10.7 %和28.6 %,接近半数样品为混合感染。并且将该方法应用于快速评价茎尖嫁接苗病毒的脱除情况。 相似文献
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东北杨树烂皮病发生流行的时空分析* 总被引：2，自引：0，他引：2
利用数理统计和空间分析方法,对2002-2009年黑龙江省、吉林省、辽宁省和内蒙古自治区杨树烂皮病发生的时间和空间特点进行分析。结果表明：近8年,东北4省（区）均有不同程度杨树烂皮病发生,其中辽宁省发生杨树烂皮病的面积最大,尤其在2006年以后,平均发生2.69万hm2/年;吉林省,黑龙江省和内蒙古自治区发生的面积较小。近2年东北4省（区）杨树烂皮病的发生均有上升趋势;东北4省区的杨树烂皮病发生地主要集中在中东部,其中吉林省的松原市近8年来,每年都有杨树烂皮病发生,平均0.360 7万hm2/年。此项研究将为林业部门和气象部门建立杨树烂皮病发生发展气象预报与灾损评估服务平台,开展该病害的预测预报和影响评价提供必要的研究基础。 相似文献
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Serological detection of Citrus psorosis virus (CPsV) by direct tissue blot immunoassay (DTBIA) and by double (DAS) and triple (TAS) antibody sandwich ELISA, was compared in samples from various citrus varieties growing in the glasshouse and in the field. In young shoots and leaves, CPsV was readily detected by the three procedures, whereas DTBIA detection in old leaves was less consistent. DTBIA detection and ELISA readings in nine different citrus varieties were similar, suggesting that CPsV accumulates to equivalent levels in all of them. In infected field trees from Spain or Italy, CPsV was consistently detected by TAS ELISA, even in samples of old leaves in winter, whereas DTBIA detection in the same trees was reliable only when using young shoots. Detection of CPsV by DTBIA and by DAS and TAS ELISA in previously untested field trees correlated perfectly with psorosis diagnostics based on biological indexing, specifically with the capacity of those sources to cross-protect against challenge inoculation with psorosis B. Some trees without bark scaling were shown to be psorosis-infected by biological indexing and to contain CPsV by serological tests; other trees showing psorosis-like bark or leaf symptoms in the field were shown to be psorosis-free by biological indexing and also CPsV-free by serology. This is the first time that the presence of CPsV has been correlated with psorosis infection as diagnosed by biological indexing. 相似文献
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Susana Martín Daniela Alioto Robert G. Milne Stephen M. Garnsey Mª Laura García Oscar Grau José Guerri Pedro Moreno 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(7):747-757
Psorosis is a citrus disease of undemonstrated etiology that can be diagnosed by biological indexing on sweet orange seedlings followed by a cross protection test. Its presumed causal agent is Citrus psorosis virus(CPsV), type species of the genus Ophiovirus. We compared detection of CPsV by ELISA, RT-PCR, molecular hybridization and immunosorbent electron microscopy, and examined its association with psorosis disease in 11 biologically characterized isolates and in 47 uncharacterized field sources by observation of field symptoms and by biological indexing including the cross protection test. Detection of CPsV by any of the four procedures always coincided with diagnosis of psorosis by cross protection, but it did not always correlate with observation of symptoms thought to be specific, in field trees or in graft-inoculated indicator plants. Trials to detect CPsV by ELISA, molecular hybridization and RT-PCR in citrus sources from different geographical origins, presumed to be psorosis-infected on the basis of field symptoms or reaction of indicator plants, were sometimes unsuccessful, indicating that psorosis symptoms may be induced by causes other than CPsV. 相似文献
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Improved detection of citrus psorosis virus using polyclonal and monoclonal antibodies 总被引：2，自引：0，他引：2
Citrus psorosis is a serious and widespread disease associated with citrus psorosis virus (CPsV), a novel filamentous negative-stranded virus in the genus Ophiovirus . Laborious and costly indexing on test plants has been the only routine diagnostic method available, but recently an antiserum usable in double antibody sandwich (DAS) ELISA has been prepared. Here, major improvements to the DAS-ELISA protocol, a new purification method, and production of two monoclonal antibodies (mabs) to CPsV, an IgG and an IgM are reported. A highly sensitive triple antibody sandwich (TAS) ELISA making use of the mabs is described. In glasshouse citrus the homologous virus was still detectable at a tissue dilution of 1/6250 in DAS and at 1/31250 in TAS-ELISA. Both the DAS and IgG mab-TAS formats detected all CPsV isolates so far tested (from Argentina, Italy, Lebanon, Spain and the USA). A few isolates were not detected by the IgM mab. 相似文献
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2005年5月到2006年4月逐月采样,运用一步法RT-PCR检测Citrus psorosis virus(CPV)在Dweet橘橙苗木叶片和枝皮中的分布。保存在控温温室中的Dweet橘橙病株中老叶、老皮、嫩叶和嫩皮全年都可以检测出CPV;保存在网室中的Dweet橘橙病株中老叶、老皮全年均能检测到CPV,而夏梢的嫩叶、嫩皮不能稳定地检测出CPV,春、秋梢的嫩叶、嫩皮均可检测到CPV,表明一步法RT-PCR检测CPV最佳取样部位为老叶和老皮。 相似文献
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A. M. D'Onghia F. Carimi F. De Pasquale K. Djelouah G. P. Martelli † 《Plant pathology》2001,50(2):266-269
Somatic embryogenesis was used to eliminate Citrus psorosis virus (CPsV) from three citrus species (common mandarin, sweet orange and Dweet tangor), all of which regenerated somatic embryos with different embryogenic potential from stigma and style explants. CPsV was detected by double antibody sandwich‐indirect‐enzyme‐linked immunosorbent assay (DASI-ELISA) in explants and embryogenic callus, but was not detected in any of the plants obtained from somatic embryos, even 24 months after regeneration. Loss of juvenile characters (disappearance of thorns) was observed in the first year of growth and was retained in plants propagated by grafting from thornless stems. Somatic embryogenesis appears to be a very promising technique for the production of healthy citrus stocks. 相似文献
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Yueping Yang Mingdu Huang G Andrew C. Beattie Yulu Xia Gecheng Ouyang Jinjun Xiong 《国际虫害防治杂志》2013,59(4):343-352
Abstract The Asiatic citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is a major pest of citrus in China. Its status derives, not from the damage it causes, but from its role as the only known vector in China of huanglongbing, a phloem-limited bacterial disease of international importance. The disease can devastate orchards within a few years of planting. It also poses a major threat to endangered indigenous citrus germplasm in Asia and Australasia. The distribution, biology, ecology and control of the psyllid in China are reviewed in these contexts. Constraints and challenges related to control of the vector in China are discussed. 相似文献
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Association of citrus psorosis B symptoms with a sequence variant of the Citrus psorosis virus RNA 2
Citrus psorosis virus (CPsV), the type species of genus Ophiovirus, is the presumed causal agent of a bark scaling disease in citrus plants. CPsV virions are kinked filaments composed of three negative‐strand RNA molecules and a ～48‐kDa coat protein. The virus induces two different syndromes: psorosis A (PsA), characterized by limited bark scaling lesions in the trunk and main limbs, and a more aggressive form of the disease called psorosis B (PsB) with rampant bark lesions affecting even thin branches and chlorotic blotches in old leaves. In the greenhouse, the PsA and PsB syndromes can be induced by graft inoculating healthy citrus seedlings with non‐lesion or with lesion bark inoculum from PsA‐affected field trees. PsA‐ and PsB‐inducing CPsV sub‐isolates obtained by this procedure from the same tree showed identical single‐strand conformation polymorphism (SSCP) profiles in homologous segments of the RNAs 1 and 3, whereas segments of the RNA 2 enabled discrimination between PsA‐ and PsB‐associated sequence variants. SSCP analysis of the RNA 2 population present in different tissues of psorosis‐infected plants showed that: (i) PsA‐inducing isolates contain PsB‐associated sequence variants at low frequency, (ii) the PsB‐associated sequence variant is predominant in blistered twigs and gummy pustules affecting old leaves, characteristic of PsB isolates, and (iii) the PsB‐associated sequence variant accumulates preferentially in bark lesions of the trunk and limbs. SSCP analysis of the RNA 2 population also enabled monitoring of interference between PsA‐ and PsB‐associated variants in plants co‐inoculated with both psorosis types. 相似文献
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2005-2007年,在南丰蜜桔上建立了以人工释放捕食螨控制柑桔害螨为核心,辅以配套的农业、物理和药剂防治措施的桔园虫害控制技术体系。试验证明该技术对防治以柑桔害螨为主的柑桔虫害效果显著。6月中旬以后,生防区红蜘蛛平均虫口密度仅为0.15头/叶,其他主要害虫如粉虱、蚧虫和蚜虫等的发生量均显著少于化防区和对照区。随着该技术的示范和推广,已逐渐展现出巨大的经济效益,每亩可节本增效600元以上,累计增加经济效益达900万元以上。同时提高了桔农素质,提升了生产水平;减少施用有毒农药60%以上,改善了生态环境,进而创造了良好的社会效益和生态效益,为加快推动南丰蜜桔无公害标准化生产进程,保障南丰蜜桔产业的可持续发展提供了技术支撑。 相似文献