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红松种子长期贮藏问题,是保存珍贵的红松种子资源及完成更新造林的重要问题,我们结合生产实际完成了红松种子在同一温度条件下不同含水量、同一含水量在不同温度条件下对种子生活力保持的试验,不同含水量的种子在不同贮藏条件下贮藏年限与生活力的关系。运用一元非线性回归法比较直观地表达了含水量、贮藏温度是贮藏的主导因素,为更好地贮藏利用红松种子提供了科学的保证。 相似文献
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为了探寻水杉种子贮藏的适宜含水量、温度和时长，以期为水杉种子的科学贮藏提供参考。本研究以水杉原产地湖北省利川市忠路镇小河村的原生水杉母树上采收的种子为试验材料，通过不同种子含水量、贮藏温度和贮藏时长试验，比较了入库和出库时种子发芽率、发芽势的变化规律。结果表明：种子含水量在10.00%～10.99%时，贮藏期间种子发芽率、发芽势的降低值最小，贮藏效果最佳。种子含水量为10.00%～10.99%,在-5℃贮藏1 a时，发芽率、发芽势降低值最小，贮藏效果最佳；-5℃贮藏1 a、2 a、3 a时，种子的发芽率、发芽势无显著性差异。因此，水杉种子贮藏入库含水量应控制在10.00%～10.99%,贮藏温度推荐-5℃,在-5℃条件下可以贮藏3 a。 相似文献
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桢楠主要通过播种的方式进行繁殖。种子贮藏方法与活力保持是播种育苗非常重要的环节。本试验以桢楠种子为材料,设置不同温度进行贮藏试验,通过研究种子发芽率、相对电导率和丙二醛含量等,初步探讨了桢楠种子的耐贮性。结果表明,桢楠种子的含水量为27.33%±1.52%。贮藏0 d时种子的发芽率为79.5%;贮藏至60 d时,25℃、4℃、-20℃、-70℃条件下种子的发芽率已经分别降低到17.5%、20%、0和0;贮藏至180 d时,4个温度条件下的发芽率全部降低为0。贮藏期间,种子的相对电导率和丙二醛含量均随时间的延长而逐渐上升。不同温度条件下,种子的相对电导率和丙二醛含量变化亦明显不同,25℃与4℃条件下的上升幅度显著小于-20℃、-70℃条件下的上升幅度,与种子发芽率的变化规律一致。可见,桢楠种子含水量为27%左右时,贮藏期间容易丧失活力。 相似文献
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[目的]通过对不同贮藏条件下香椿种子发芽指标、抗氧化酶活性等测定分析,探索种子含水量和贮藏温度对香椿种子生理生化特性的影响,为香椿种子的贮藏方法提供技术支持。[方法]利用100%湿度环境获得9.1%、12.2%、14.0%、15.8%和17.8%五个含水量的香椿种子,在10、15、和20℃下分别贮藏90 d,每30 d测定种子发芽率和抗氧化酶活性等指标。[结果]在相同贮藏条件下,随贮藏时间延长,种子发芽率、活力指数、过氧化氢酶(CAT)和过氧化物酶(POD)活性逐渐下降,超氧化物歧化酶(SOD)活性先升后降,浸出液电导率和丙二醛(MDA)逐渐上升。经90 d贮藏,随种子含水量或贮藏温度的升高,香椿种子发芽率和活力指数呈下降趋势,浸出液电导率和MDA含量显著上升,种子中SOD、POD和CAT活性均随贮藏时间的延长而显著下降。[结论]种子浸出液电导率和MDA含量与香椿种子发芽率呈显著负相关,抗氧化系统酶活性与发芽率呈显著正相关,膜质过氧化作用是引起香椿种子老化劣变的重要原因之一。本研究得出的香椿种子安全贮藏条件为:当温度不超过15℃时,含水量应控制在12.2%以下,当温度在15～20℃时,含水量应低于9.1%。 相似文献
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【目的】为探究贮藏温度对南京椴种子耐贮性的影响,揭示不同贮藏温度条件下种子发芽率和生理生化指标的变化规律,旨在探寻种子适宜贮藏温度,为南京椴种子贮藏提供科学依据。【方法】以含水量为9.80%的南京椴种子为试验材料,存放在室温（23～27℃）、4、-5和-40℃等4种温度条件下开展种子贮藏,对贮藏0、180、360、540、720 d的种子分别测定种子绝对发芽率和相对电导率、SOD活性、POD活性、 O2-·含量、OH·产生能力与MDA含量等生理生化指标。【结果】贮藏温度和贮藏时间协同影响南京椴种子活力。随着贮藏时间的延长,各贮藏温度条件的种子发芽率均下降;随着贮藏温度的下降,种子发芽率显著上升;至720 d贮藏试验结束时,在室温、4、-5和-40℃贮藏条件下的种子绝对发芽率分别为43%、49%、60%、62%。贮藏时间延长导致种子发生不同程度的劣变,不同贮藏温度条件下的种子相对电导率均呈上升趋势;在贮藏前期,种子SOD和POD活性升高,后期均有所下降;种子O2-·含量、OH·产生能力和MDA含量整体均呈逐渐上升的变化趋势。【结论】室温贮藏180 d时,南京椴种子仍可保持较高的活力水平;... 相似文献
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《热带林业》2018,(4)
研究了5℃、12℃、25℃条件下贮藏和发芽过程中油茶种子中可溶性蛋白质、脂肪、可溶性总糖含量的变化,得出以下结论:3个月内,不同温度下贮藏的种子中可溶性总糖含量呈减少趋势、脂肪含量呈增加趋势、可溶性蛋白质含量呈下降趋势,但各内含物在三种温度下的变化速率不同;在贮藏过程中,可溶性总糖和脂肪含量变化呈线性相关关系。3种贮藏温度下,脂肪含量与可溶性总糖含量呈显著负相关,脂肪含量与可溶性蛋白质含量呈负相关;可溶性蛋白质和可溶性总糖的含量正相关。种子萌发过程中,可溶性总糖含量上升、脂肪含量下降、可溶性蛋白的含量前期变化不明显,而后迅速下降。油茶种子萌发过程中,脂肪含量与可溶性总糖含量呈显著负相关,脂肪含量与可溶性蛋白质含量呈正相关;可溶性蛋白质和可溶性总糖的含量呈负相关。 相似文献
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[目的]以枫香种子为试材,研究不同贮藏温度下种子活力随时间的变化规律,为枫香种子的贮藏方法提供理论依据。[方法]将枫香种子阴干至含水量为10.54%,然后分别贮藏于室温(25±2)℃、4℃、-20℃和-70℃的温度条件下,于贮藏0、60、180、300、360、520、730 d分别测定种子的丙二醛含量和相对电导率,并将种子于室内播种,测定发芽势、发芽率及播种1个月后萌发幼苗的苗高和根长。[结果]随着贮藏时间的延长,4种温度下种子的发芽率、发芽势、幼苗苗高与根长均逐渐降低,丙二醛含量和相对电导率逐渐升高;贮藏至360 d时,4种温度均能较好地保持种子活力,相互之间差异不明显;至730 d时,4℃、-20℃和-70℃的贮藏效果明显优于室温,-70℃的贮藏效果最佳。[结论]枫香种子在含水量10.54%时,若贮藏时间在360d以内,则室温贮藏即可有效保持种子活力;若贮藏时间进一步延长,则需要采用低温进行贮藏;贮藏730d时,4℃、-20℃和-70℃均可有效保持种子活力,其中,-70℃的贮藏效果最佳。 相似文献
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以山核桃为研究对象,探讨不同温度和初始含水量对山核桃贮藏过程中油脂氧化、类脂褐素(LFLP)积累、多酚含量及醇提物还原力的影响.结果表明:类脂褐素的积累量随着贮藏时间的延长呈上升趋势,多酚含量及醇提物还原力呈下降趋势.在40℃贮藏时,含水量高(12％和16％)的山核桃原料类脂褐素积累速度略低于含水量低(4％和6％)的山核桃;但贮藏温度为25℃时,含水量高的类脂褐素积累速度高于含水量低的山核桃.油脂初级氧化产物和二级氧化产物与贮藏温度和初始含水量呈现复杂关系,40℃贮藏时,含水量高(12％和16％)的山核桃原料油脂氧化程度低于含水量低(4％和6％)的,但贮藏温度为25℃时,含水量高的油脂氧化程度高于含水量低的.多酚含量随含水量的增加和温度的升高损失速度加快,醇提物的还原力变化规律与此类似.总的来说,较低的贮藏温度和低初始含水量能有效地抑制类脂褐素的生成,延缓山核桃的衰老进程. 相似文献
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为解决竹子种子长期保存问题,以马来龙竹、云南龙竹、巨龙竹、金平龙竹、麻竹、长节勒竹、云南箭竹、棉花竹、爪哇巨竹、大泰竹、笻竹、方竹和毛竹13种竹子的成熟种子为材料,通过研究不同低温、低温下不同储存时间、不同含水量、不同发芽设施和不同基质对种子发芽率的影响,来判断竹子种子保存的适合条件。结果表明：1）除笻竹和方竹的种子外,大多数竹种的种子在低温储存5个月内,随着温度由10℃逐渐降低到-16℃,种子发芽率会有不同程度的增高;2）在-16℃条件下,随着储存时间的延长,种子发芽率会有所下降;3）种子含水量会在很大程度上影响种子的发芽率,含水量超过在一定临界值,随着含水量的增加,种子发芽率会显著下降;4）选择不同的种子发芽设施也会影响种子发芽率,生产中宜选用具有微喷灌条件的温室进行育苗;5）在无菌红土、黑土和蛭石这3种基质中,蛭石是竹子播种育苗的适宜基质。 相似文献
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Elissavet Konstantinidou Ioannis Takos Theodora Merou 《European Journal of Forest Research》2008,127(2):125-131
The effect of seed moisture content (m.c.) and seed storage conditions of bay laurel (Laurus nobilis L.) was investigated in relation to seed viability. In the first experiment, the effect of drying rate on seed moisture and
seed germination was investigated. Fresh seeds, with their original moisture content displayed a germination percentage of
55.1%. When the seed moisture content was reduced by 2.0% in an oven, the germination percentage rose to 81.0%. When the seed
moisture content was reduced even more by using the same method, the germination percentages decreased dramatically. Reducing
the seed moisture content to 28.7 and 23.5% by drying the seeds in alternating room conditions resulted in an increase of
seed germinability to 84.3 and 90.9%, respectively. The drying of the seeds for 45, 60 and 75 days reduced their seed germination
to 66.8, 49.4 and 48.0%, respectively. Reducing seed moisture content below 15.0% resulted in practically nullifying seed
germinability. The fact that bay laurel seeds cannot retain their germinability at lower moisture contents demonstrates that
it is a species with recalcitrant seeds. In the second experiment, moist and dry storage conditions were tested under different
temperatures and moisture contents. The storage experiment showed that the most effective way of conserving the bay laurel
seeds is moist storage at 0 ± 1°C for 4 months without previous drying of the seeds. 相似文献
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采用正交设计从种子含水量、包装方法、保存温度和预回湿方法4个因素来探索山合欢种子超干燥保存的可行性。用硅胶将种子脱水至1.42%～6.35%6个含水量梯度,结合不同处理密封保存1年,随后测定发芽率和一些生理生化指标,包括相对电导率(RC)、脯氨酸(Pro)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、可溶性总糖含量和脂肪酸组分。结果表明:种子含水量和预回湿方法对发芽率有显著影响,超干种子与未经超干处理的种子相比,发芽率有明显提高;超干种子发芽率与相对电导率和丙二醛含量呈显著负相关,与脯氨酸含量和3种抗氧化酶活性呈显著正相关;超干种子的油酸和α-亚麻酸含量比对照提高。适宜的超干处理能使抗氧化酶活性增强,使不饱和脂肪酸和可溶性总糖的含量增加,从而减缓种子生活力下降。 相似文献
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Truong Mai-Hong Tran D. Hong Nguyen T. Hien Ho H. Hai Tran D. Tung Vo T. Le-Tam Banh Ngoc-Tam Richard H. Ellis 《New Forests》2006,32(1):9-19
Mass maturity (end of the seed-filling phase) occurred at about 72 days after flowering (DAF) in developing seeds of Mimusops elengi, at which time seed moisture content had declined to about 55%. The onset of ability to germinate was detected at 56 DAF
and seeds showed 98% germination by 84 DAF. Tolerance of desiccation to 10% moisture content was first detected at 70 DAF
and was maximal by 84 DAF. Delaying collection by a further 14 days to 98 DAF, when fruits began to be shed, reduced seed
viability, particularly for seeds first dried to 10% moisture content. Hence the best time for seed collection appears to
be about 14 days before fruits shed. In a separate investigation with six different seed lots, desiccation below about 8–12%
moisture content reduced viability (considerably in some lots). The viability of dry seeds (below about 10% moisture content)
stored hermetically was reduced at cool temperatures (5 °C and below), and none survived storage at sub-zero temperatures.
The results suggest that Mimusops elengi shows intermediate seed storage behaviour and that the optimal hermetic seed storage environment is about 10% moisture content
at 10 °C, while short-term, moist, aerated storage at high (40%) moisture content is also feasible. 相似文献
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V. Sivakumar R. Anandalakshmi R. R. Warrier M. Tigabu P. C. Odén S. N. Vijayachandran S. Geetha B. G. Singh 《New Forests》2006,32(2):121-131
Seeds of Strychnos nux-vomica have slow and erratic germination; thus different presowing treatments were applied to enhance the germination of its seeds
collected from Tamaraikulam, Tamil Nadu, India. In addition, the effects of desiccation and different storage conditions on
the germination of S. nux-vomica seeds were investigated. The results show that soaking in 500 ppm gibberellic acid (GA3) for 24 h, incubation of seeds at 40 °C for 3 days and alternate water soaking (16 h) and drying (8 h) for 14 days significantly
increased the percentage germination compared to the control. Desiccation of seeds down to 10% moisture content resulted in
better germination. Germination of S. nux-vomica seeds differed significantly between different storage periods, moisture contents of the seed and for first and second order
interactions (p<0.001). The highest germination (92%) was achieved when seeds with 10% moisture content were stored at ambient temperature
for 30 weeks. Evidence from the present study indicates that S. nux-vomica seeds possess physiological dormancy that can be broken effectively by after-ripening. As seeds of S. nux-vomica are found to be desiccation tolerant, dry seed (10% moisture content) can be hermitically stored at ambient temperature for
30 weeks without losing their viability. 相似文献
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We performed desiccation and storage trials to better under- stand storage behavior of Knema attenuata seeds. Mature seeds with moisture content (MC) of 31% exhibited 73% germination. During the period of desiccation (open lab condition) seeds with MC 23% showed 40% germination. After further drying to MC 21% germination was reduced to 16%. Complete loss in viability resulted when seed moisture was reduced to 18%. The seeds stored at -10°C, 0°C, 10°C and 28±2°C (open lab condition) lost their viability within 10 days. Seeds stored in sealed polythene bags and moist sand retained viability for more days than did seeds stored under all other storage conditions. Sensitivity of seeds to lower temperature and desiccation suggest that the storage be- havior of K. attenuata seeds is recalcitrant. Seeds stored in moist condi- tions can, at best, be stored for a period of two months. 相似文献
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Storage of non-dormant seeds of Fraxinus angustifolia Vahl 总被引：1，自引：0，他引：1
The effects of drying and storage on the quality of Fraxinus angustifolia non-dormant seeds have been investigated. Mature seeds subjected to a 2-week warm stratification (20 °C) followed by a 2-week cold stratification (3 °C), were exposed to a gentle air stream at room temperature to dry them to 9.5% moisture content. After 4, 8 and 12 months of storage at –3 °C, in hermetically sealed containers, germination tests were conducted in darkness at 30/20 °C and 25/5 °C to evaluate possible changes in seed quality. The results obtained suggest the feasibility of drying non-dormant seeds. In fact, though causing a slight decrease in seed quality, such a process enables storage of ready to sow seeds for at least 12 months without any further loss. The temperature regime of 30/20 °C, prescribed by the International Seed Testing Association (ISTA), was found to be suboptimal for the particular conditions tested in this trial, the 25/5 °C cycle being more stimulating for germination. 相似文献