共查询到20条相似文献，搜索用时 359 毫秒
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樗蚕越冬代雌蛹重与产卵量关系研究 总被引：1，自引：0，他引：1
樗蚕越冬代雌蛹重与产卵量关系研究韩会智宋华茹张秀红任淑萍（沧州市林业局,０６１００１）刘侠李振刚（沧县农林局）（任丘市林业局关键词雌蛹重;产卵量;樗蚕中图分类号Ｓ７６３．４２ＲＥＬＡＴＩＯＮＳＨＩＰＢＥＴＷＥＥＮＰＵＰＡＷＥＩＧＨＴＡＮＤＬＡＩＤＥＧ... 相似文献
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中国海南省茶秆竹属一新种符国瑗（海南省林业局标本室海口５７０２０３）ＡＮＥＷＳＰＥＣＩＥＳＯＦＰＳＥＵＤＯＳＡＳＡＦＲＯＭＨＡＩＮＡＮＰＲＯＶＩＮＣＥＣＨＩＮＡ￥ＦｕＧｕｏａｉ（ＦｏｒｅｓｔｒｙＢｕｒｅａｕｏｆＨａｉｎａｎ，Ｈａｉｋｏｕ５７０２０３）... 相似文献
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通过对分布于欧洲的水青冈（Ｆａｇｕｓｓｙｌｖａｔｉｃａ和Ｆａｇｕｓｏｒｅｎｔａｌｉｓ）和亚洲的水青冈（Ｆａｇｕｓｊａｐｏｎｉｃａ，Ｆａｇｕｓｃｒｅｎａｔａ，Ｆａｇｕｓｌｕｃｉｄａ，Ｆａｇｕｓｅｎｇｌｅｒｉａｎａ和Ｆａｇｕｓｐａｓｈａｎｉｃａ）的地理历史资料分析和凝胶电泳法等位酶的测定，探讨欧亚大陆水青冈地理变异和遗传多样性．所测定的酶系统包括：过氧化物酶（ＰＸ１和ＰＸ２）、磷酸葡萄糖脱氢酶（ＰＧＤ）、酸性磷酸化酶（ＡＣＰ１和ＡＣＰ２）、超氧化物歧化酶（ＳＯＤ）、谷氨酸草酰乙酸转氨酶（ＧＯＴ１，ＧＯＴ２和ＧＯＴ３）、异柠檬酸脱氢酶（ＩＤＨ）、磷酸果糖异构酶（ＰＧＩ）、甲基萘醌还原酶（ＭＮＲ）、葡萄糖磷酸变位酶（ＰＧＭ１和ＰＧＭ２）和苹果酸脱氢酶（ＭＤＨ１和ＭＤＨ２）１０种酶系统．测定和分析了水青冈遗传相似性、固定指数及遗传多样性随经度、纬度和海拔高度的变化规律，讨论了水青冈起源和分布特点，为进一步研究水青冈的种间关系和地理历史进化过程提供了科学依据． 相似文献
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小叶杨原生质体培养植株再生及其同工酶的变化 总被引：4，自引：2，他引：4
从小叶杨（Ｐｏｐｕｌｕｓｓｉｍｏｎｉｉ）胚性悬浮细胞分离得到大量有活力的原生质体，产量高达３．８×１０７ｇ－１ＦＷ。高密度（３×１０６／ｍＬ）液体浅层培养可促进小叶杨原生质体的分裂和生长；以葡萄糖为唯一碳源的Ｋｍ８ｐ培养基，有利于原生质体的持续分裂；在培养基中添加有机氮，有助于提高原生质体的植板率。原生质体形成细胞团后，逐步降渗和分皿培养，能有效促进细胞团的持续分裂。愈伤组织在不同分化培养基上的分化频率存在着明显的差异，同工酶分析说明，分化能力强的愈伤组织，其ＴＣＡ循环和磷酸戊糖途径较为活跃，ＧＯＴ、ＥＳＴ和ＰＥＲＯＸ的活性比较高。当芽伸长到２—３ｃｍ时，从基部切下转移到无激素的１／２ＭＳ培养基上诱导生根并形成完整植株。移入盒内，在温室生长良好。 相似文献
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山杨二元材积模型的研究陈东来，秦淑英（河北林学院林学系保定０７１０００）关键词山杨，二元材积模型，拟合中图分类号Ｓ７５８．５１ＡＳＴＵＤＹＯＦＳＴＡＮＤＡＲＤＶＯＬＵＭＥＭＯＤＥＬＳＦＯＲＰＯＰＬＡＲ（ＰＯＰＵＬＵＳＤＡＶＩＤＩＡＮＡ）ＣｈｅｎＤｏｎ... 相似文献
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苹果有袋栽培的发展前景 总被引：2，自引：0，他引：2
苹果有袋栽培的发展前景陈彦同（河北省无极县林业局，０５２４６０）关键词苹果；有袋栽培；无袋栽培；果实品质中图分类号Ｓ６６１．１ＰＲＯＳＰＥＣＴＯＦＢＡＧＧＩＮＧＦＲＵＩＴＳＩＮＡＰＰＬＥＯＲＣＨＡＲＤＳＣｈｅｎＹａｎｔｏｎｇ（ＦｏｒｅｓｔｒｙＢｕｒ... 相似文献
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猕猴桃试管苗愈伤组织诱导及叶片和茎段的再生 总被引：3，自引：0，他引：3
在猕猴桃的组织培养中,为提高繁殖系数,以茎段作外植体,在添加 １０ｕＭＢＡＰ的改 良ＧＤ培养基上进行愈伤组织诱导,诱导率最高为 １００％,用 ＭＳ＋ＢＡＰ１０ ＵＭ＋ＩＢＡ ５ ｕＭ愈伤组织的继代培养,一次可提高愈伤组织的量的１０％左右。在添加１５ ＵＭＢＡＰ的改良ＧＤ培养基上进行分化培养,愈伤组织分化率可达 １００％,平均 １０ ｍｇ大小的愈伤组织可产生 ４个小 苗,以猕猴的叶片,茎段作外植体,在添加１０ｕＭＢＡＰ＋ＩＢＡ ５ｕＭ的 ＭＳ培养基上进行不定 芽的分化诱导,平均１ｃｍ长的茎段可诱导 ５～６个不定芽,１． ５ ｃｍ×１． ５ ｃｍ的叶片可诱导 １０～１５个不定芽。然后把愈伤组织产生的小苗和叶片,茎段诱导产生的不定芽接种在芽分化及 生根培养基上［１］即可成苗。 相似文献
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杨树皮储藏蛋白基因启动子的克隆和功能研究 总被引：17，自引：0，他引：17
杨树树皮储藏蛋白BSP是类似种子储藏蛋白的氮素储藏物 ,冬季在韧皮部薄壁细胞中大量积累 ,是落叶树氮代谢中的重要成分。为了研究BSP基因启动子在转基因植物中的表达特性 ,探索其在植物基因工程研究中潜在的应用价值 ,我们用PCR方法从美洲黑杨基因组中DNA扩增得到了BSA启动子片段。与GUS基因融合构建中间载体后 ,转化烟草 ,获得了一批PCR检测为阳性的转化再生植株。经GUS组织化学检测 ,发现若干转基因烟草的茎和叶柄韧皮部以及叶脉都呈GUS染色阳性 ,初步证明杨树BSP基因启动子确有韧皮部表达特性 ,可介导GUS基因在转基因烟草韧皮部特异表达。 相似文献
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Agrobacterium tumefaciens strain LBA 4404 carrying pBI121 plasmid was used to transform mature zygotic embryos of three genotypes (E-Hb, E-Ma, and
E-Mc) of loblolly pine. The results demonstrated that the expression frequency of β-glucuronidase reporter gene (GUS) varied
among genotypes after mature zygotic embryos were infected withAgrobacterium tumefaciens cultures. The highest frequency (27.8%) of GUS expressing embryos was obtained from genotype E-Mc with mean number of 21.9
blue GUS spots per embryo. Expression of β-glucuronidase reporter gene was observed on cotyledons, hypocotyls, and radicles
of transformed mature zygotic embryos, as well as on organogenic callus and regenerated shoots derived from co-cultivated
mature zygotic embryos. Nineteen regenerated transgenic plants were obtained from GUS expression and kanamycin resistant calli.
The presence and integration of the GUS gene was confirmed by polymerase chain reaction (PCR) and Southern blot analysis.
These results suggested that an efficientAgrobacterium tumefaciens-mediated transformation protocol for stable integration of foreign genes into loblolly pine has been developed and that this transformation
system could be useful for the future studies on transferring economically important genes to loblolly pine. 相似文献
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Transgenic sterility in Populus: expression properties of the poplar PTLF, Agrobacterium NOS and two minimal 35S promoters in vegetative tissues 总被引：2，自引：0，他引：2
Transgenic sterility is a desirable trait for containment of many kinds of transgenes and exotic species. Genetically engineered floral sterility can be imparted by expression of a cytotoxin under the control of a predominantly floral-tissue-specific promoter. However, many otherwise desirable floral promoters impart substantial non-floral expression, which can impair plant health or make it impossible to regenerate transgenic plants. We are therefore developing a floral sterility system that is capable of attenuating undesired background vegetative expression. As a first step towards this goal, we compared the vegetative expression properties of the promoter of the poplar (Populus trichocarpa Torr. & Gray) homolog of the floral homeotic gene LEAFY (PTLF), which could be used to impart male and female flower sterility, to that of three candidate attenuator-gene promoters: the cauliflower mosaic virus (CaMV) 35S basal promoter, the CaMV 35S basal promoter fused to the TMV omega element and the nopaline synthase (NOS) promoter. The promoters were evaluated via promoter::GUS gene fusions in a transgenic poplar hybrid (Populus tremula L. x P. alba L.) by both histochemical and fluorometric GUS assays. In leaves, the NOS promoter conveyed the highest activity and had a mean expression level 5-fold higher than PTLF, whereas the CaMV 35S basal promoter fused to the omega element and the CaMV 35S basal promoter alone directed mean expression levels that were 0.5x and 0.35x that of PTLF, respectively. Differential expression in shoots, leaves, stems and roots was observed only for the NOS and PTLF promoters. Strongest expression was observed in roots for the NOS promoter, whereas the PTLF promoter directed highest expression in shoots. The NOS promoter appears best suited to counteract vegetative expression of a cytotoxin driven by the PTLF promoter where 1:1 toxin:attenuator expression is required. 相似文献
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The 9-cis-epoxycarotenoid dioxygenase(NCED)gene is rate-limiting in abscisic acid(ABA) biosynthesis.In this study, an NCED gene, designated FvNCED3(KY008746), was cloned from velvet ash(Fraxinus velutina Torr.) with a RACE method. The full length c DNA of FvNCED3 encodes a 573-amino acid polypeptide.Sequencing analysis showed that the FvNCED3 protein was highly homologous to other NCED proteins. The expression patterns of FvNCED3 in different ash organs were analyzed by real-time PCR which revealed that FvNCED3 expression levels were highest in leaves and lowest in roots. The gene expression patterns of FvNCED3 under abiotic stress indicated that its expression increased under drought, salt and ABA stress and decreased due to high and low temperatures. There were no obvious changes under ultraviolet light. The 1094-bp upstream sequence 5' flank regulation region of the FvNCED3 gene was also cloned from ash using the Genome Walking method. To assess the activity of the FvNCED3 promoter, a p FvNCED3 p::GUS plant expression vector was constructed for tobacco transformation. GUS expression of the FvNCED3 GUS enzyme activity was detected in almost all transgenic tobacco tissues, especially in the young leaves,stigma, anther, ovule and ovary. After treating the transgenic tobacco with NaCl and placing it under drought stress, GUS staining of tobacco leaves increased compared with that under normal growth conditions. This result indicates that gene expression driven by the FvNCED3 promoter can be induced by salt and drought stress. 相似文献
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A 3 125 bp cellulose synthase gene, PtoCesA1, which has a 98% identity to PtrCesA1 from Populus tremuloides, was cloned from cDNA prepared from secondary xylem of P tomentosa. Four anti-expression vectors with different fragments of PtoCesAl, named as pBIPF, pBICC1, pBIPR and pBIBR, were constructed. Some traits of transformed tobacco of pBICC1, pBIPR and pBIBR differed from wild types, such as small leaves, ＂dwarf＂ phenotype and thinner xylem and fiber cell walls than wild plants consistent with a loss of cellulose. It indicated that the growth of transgenic tobacco was restrained by the expression of anti-PtoCesA1. Transgenic tobacco was obtained and the contents of cellulose and lignin were analyzed as well as the width and length of fiber cells, and xylem thickness for both transgenic and control plants. Transformed tobacco showed a different phenotype from control plants and it implied that PtoCesA1 was essential for the cellulose biosynthesis in poplar stems. 相似文献
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多基因转化是基因工程研究热点之一。本研究应用DNA重组技术,将两个抗病机制不同,抗菌谱较广的抗病基因(天麻抗真菌蛋白GAFP和兔防御素NP1基因)构建在一个植物表达载体pBin35SGAFP-NP1上,两者具有各自的CaMV35S启动子和Nos终止子。通过根癌农杆菌介导,采用叶盘法转化烟草,PCR和PCR-Southern分析证明已将NP1和GAFP基因整合到烟草基因组中。离体抑菌实验表明转基因植株对真菌和细菌表现出一定的抗性。以上结果表明通过该表达载体进行遗传转化可获得含双价抗病基因植物,并能有效表达,提高转基因植物抗病能力。 相似文献
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Genetic transformation of loblolly pine using mature zygotic embryo explants by Agrobacterium tumefaciens 总被引：1，自引：0，他引：1
Introduction1Genetictransformationinconifershasthepotentialtoallowtheselectiveimprovementofindividualtraitsinelitecloneswhilestillmaintainingtheexistingcombinationofgenesresponsibleforthesuperiorphenotype(Charestetal.1991;Jamesetal.1996;Walteretal.1999).Atpresent,althoughconsiderableresearchefforthasbeendevotedtothegeneticengineeringofconiferspecies(Sederoffetal.1986;Bekkauoietal.1988,1990;Robertsonetal.1992;Bomminenietal.1993;Shinetal.1994;Klimaszewskaetal.1997),ithaslaggedbehindadvancesma… 相似文献
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PtLFY, a LEAFY （LFY） gene, was cloned from Populus tomentosa （LM50） by PCR. Sequencing analysis indicated that PtLFY was 2629 bp long, composed of three exons and two introns and encoded 378 amino acids. The splice donor sites and the splice acceptor sites were in identical positions to the LFY and its homologues. The amino acid sequence inferred was 68%-99% homologous to those of LFY and its homologues by blast analysis in GenBank. The Southern blot analysis indicated that there was a single copy of the PtLFY gene in genomic DNA of male and female P. tomentosa （LM50 and 5082）. The pBI121-Ptalfy （reverse）-intron-Ptlfy-GUS-nos was constructed using RNA interference （RNAi） technique and verified by PCR and digestion identification and transformed into tobacco. Some transgenic tobacco plants were obtained by PCR and PCR-Southern identification. The growth was generally repressed in transgenic tobacco plants compared with wild-type ones and some phenotypic differences were observed. 相似文献
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植物基因工程及其在林木害虫防治上的应用 总被引：5，自引：2，他引：5
植物基因工程主要包括目的基因的分离、基因工程载体的构建、植物细胞的遗传转化、转化细胞的组织培养和植株再生、外源基因表达的检测等几个方面。利用该技术目前已转化了20多种林木树种,并成功地将抗虫的苏云金芽杆菌内毒素基因和蛋白酶抑制剂基因转入林木树种。 相似文献
