Development of broad‐spectrum bacterial blight resistance into thermo‐sensitive genic male sterile lines          下载免费PDF全文

Jiefeng Jiang  Jiaming Mi  Jauhar Ali  Tongmin Mou 《Plant Breeding》2016,135(1):73-79

Two‐line hybrid rice technology is an effective way to increase rice production and improve rice quality. In this study, three bacterial blight (BB) resistance genes, Xa7, Xa21 and Xa23, were introgressed separately into C815S, a popular thermo‐sensitive genic male sterile (TGMS) line to develop five BB‐resistant lines (Hua1005S, Hua1002S, Hua1009S, Hua1006S and Hua1001S) to be resistant against seven races of Xanthomonas oryzae pv. oryzae (Xoo). The two‐line hybrids with heterozygous Xa23 were resistant against seven Xoo strains. But, the hybrids with heterozygous loci for both Xa7 and Xa21 were only resistant against three Xoo strains and were moderately susceptible to the other four strains indicating the role of modifiers influencing the poor expression of dominant BB resistance genes under heterozygous state. Among them, Hua1006S was found to be a promising TGMS line with its higher degree of disease resistance level on account of broad‐spectrum resistance gene Xa23 besides possessing better plant type and rice grain quality features.  相似文献   

温敏核不育系株1S籼粳属性研究     

刘平  戴小军  杨远柱  李文嘉  欧立军  梁满中  陈良碧 《作物学报》2008,34(12):2112-2120

温敏核不育系株1S是生产上广泛应用的水稻优良早籼型不育系品种。以典型籼稻和粳稻为对照,采用ISSR、SRAP和TRAP三种分子标记方法对株1S核DNA进行分子遗传学分析,结果表明,株1S核DNA以籼型基因为主,但含有部分粳型特异性片段,具有一定的粳型血缘;3种分子标记方法都能建立株1S所特有的分子指纹。对株1S叶绿体DNA中ORF100、ORF29-TrnC^GCA、TrnT^UGU–TrnL^UAA、rps16基因内含子等序列分析表明,株1S为籼型叶绿体,对照材料培矮64S、准S为粳型叶绿体;株1S在TrnT^UGU–TrnL^UAA片段中存在两个特异碱基。利用籼型细胞质和含有适量粳稻血缘的两用核不育系可能是长江中下游双季稻区高产稳产早稻组合的育种途径。  相似文献   

Mapping of male sterility gene ms10 in chilli pepper (Capsicum annuum L.)          下载免费PDF全文

Parjeet Singh Aulakh  Major S. Dhaliwal  Salesh Kumar Jindal  Roland Schafleitner  Kuldeep Singh 《Plant Breeding》2016,135(4):531-535

Most of the hybrid seed in chilli are produced manually, but the use of male sterility (MS) can reduce the cost of hybrid seed production. MS‐12, a nuclear male‐sterile (NMS) line developed at Punjab Agricultural University, Ludhiana (India), has been utilized to develop commercial F₁ hybrids. A recessive gene, designated as ms10, governs MS in MS‐12. Due to recessive gene control, development of new NMS lines incorporating ms10 gene is tedious and time‐consuming. We identified SSR markers AVRDC‐PP12 and AVRDC_MD997* linked to the ms10 gene. A total of 558 primer pairs were screened following bulked segregant analysis (BSA). Linkage analysis in 210 F₂ plants indicated that the two SSR markers were linked to the ms10 gene and the marker AVRDC‐PP12 was closest to the gene at 7.2 cM distance. The marker was mapped to chromosome 1 at genome position 175 694 513 to 175 694 644. Until more closely linked markers are developed, the marker AVRDC‐PP12 would facilitate transfer of ms10 gene through marker‐assisted selection (MAS). Fine mapping would lead to cloning of the ms10 gene.  相似文献   

水稻新质源(CMS-FA)雄性不育恢复基因的遗传研究     

王乃元  梁康迳  李毓  王颖  王洪飞  仇秀丽 《作物学报》2008,34(11):1929-1937

发掘水稻新型雄性不育细胞质源CMS-FA,育成系列优质米不育系和系列新质源恢复系,组配成强优势杂交稻组合的基础上研究新质源雄性不育恢复系的恢复基因遗传。采用新质源(CMS-FA)不育系金农1A与恢复系金恢3号杂交获得杂交F₁代种子,种植F₁代,收获自交F₂代种子。用F₁分别与不育系或保持系回交,获得(不育系//不育系/恢复系和不育系/恢复系//保持系)2个测交群体。同时种植P₁、P₂、F₁、F₂、B₁F₁和B₂F₁等群体,考察花粉染色率、套袋结实率和自然结实率,卡平方测验遗传分离适合度。结果表明,不育系与恢复系杂交F₁代正常可育,育性恢复(可育)基因为显性遗传。F₂代分离出可育︰不育适合3︰1,育性恢复(可育)基因为1对显性基因控制。B₁F₁和B₂F₁代2个测交群体的可育︰不育都适合1︰1分离规律,验证了F₂代育性恢复(可育)单基因的遗传模式。暂时确定新质源(CMS-FA)核质互作三系的基因型为不育系S(SS)、保持系F(SS)和恢复系S(FF)。  相似文献   

Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus)   总被引：7，自引：1，他引：7  

G. Y. Lu    G. S. Yang  T. D. Fu 《Plant Breeding》2004,123(3):262-265

Rs1046AB is a genic male sterile two‐type line in rapeseed that has great potential for hybrid seed production. The sterility of this line is conditioned by the interaction of two genes, i.e. the dominant genic male sterility gene (Ms) and the suppressor gene (Rf). The present study was undertaken to identify DNA markers for the Ms locus in a BC₁ population developed from a cross between a male‐sterile plant in Rs1046AB and the fertile canola‐type cultivar ‘Samourai’. Bulked segregant analysis was performed using the amplified fragment length polymorphism (AFLP) methodology. From the survey of 480 AFLP primer combinations, five AFLP markers (P10M13₃₅₀, P13M8₄₀₀, P6M6₄₁₀, E7M1₂₃₀ and E3M15₁₀₀) tightly linked to the target gene were identified. Two of them, E3M15₁₀₀ and P6M6₄₁₀, located the closest, at either side of Ms at a distance of 3.7 and 5.9 cM, respectively. The Ms locus was subsequently mapped on linkage group LG10 in the map developed in this laboratory, adding two additional markers weakly linked to it. This suite of markers will be valuable in designing a marker‐assisted genic male sterility three‐line breeding programme.  相似文献   

Conversion of the genic male sterility (GMS) system of bell pepper (Capsicum annuum L.) to cytoplasmic male sterility (CMS)     

Shu‐Yun Chen  Szu‐Chien Liu  Tzu‐Chuan Lo  Andi Wahyono  Sz‐Yun Chen  Hsin‐Mei Ku 《Plant Breeding》2014,133(2):291-297

Non‐pungent bell pepper (Capsicum annuum L.) lacks the cytoplasmic male sterility (CMS) nuclear restorer allele, Rf, and CMS cannot be employed in its F₁ hybrid seed production. To demonstrate that the genic male sterility (GMS) system in non‐pungent bell pepper can be converted to the CMS male sterility system, the conversion of GMS to CMS for non‐pungent bell pepper line GC3 was conducted by introgression of S‐type cytoplasm and the Rf allele from tropical pungent donors. After morphological traits were evaluated, two lines from BC₁F₁ containing S‐type cytoplasm and four lines from BC₂F₂ containing Rf allele, phenotypically similar to GC3, were obtained and could be employed as CMS male sterile lines and restorer lines for non‐pungent bell pepper. Four molecular markers potentially linked to traits of interest were also evaluated in BC₁F₁ and BC₁F₂ populations. This is the first time that GMS has been successfully converted to CMS in bell pepper, a significant contribution for bell pepper hybrid seed production.  相似文献   

Identification and gene cloning of a male‐sterile mutant Oswbc11 in rice     

Changqing Xuan  Yaping Liu  Chengjie Liu  Lewis Bongani‐Tafadzwa Lusuwi  Xiaojin Fu  Xifeng Chen  Bojun Ma 《Plant Breeding》2019,138(3):290-298

Commercial application of nuclear genetic male sterility has been improved recently by a novel technique, Seed Production Technology (SPT), which incorporates transgenic maintainer lines capable of propagating non‐transgenic nuclear male‐sterile lines for use as female parents in hybrid production. Here, we identified a rice nuclear male‐sterile mutant, Oswbc11, with abnormal pollen development and lipid transport. We finely mapped the Oswbc11 gene into a 12.5‐kb region on chromosome 10 and found one candidate gene, which had a base substitution (C to T) resulting in a premature stop codon and was functionally confirmed by CRISPR/Cas9 technology. Gene OsWBC11 encodes a sub‐family member of adenosine triphosphate‐binding cassette transporter, which participates in the active transport of a wide range of molecules across membranes. Moreover, the agronomic traits of Oswbc11 mutants showed no significant differences compared to the wild‐type control except for the seed setting rate. These results indicated that Oswbc11 gene could be used in rice hybrid breeding as a recessive nuclear male‐sterile gene combined with CRISPR/Cas9 and the SPT technology and applied in different rice varieties.  相似文献   

Identification of AFLP markers linked to the epistatic suppressor gene of a recessive genic male sterility in rapeseed and conversion to SCAR markers   总被引：2，自引：0，他引：2  

Y. Z. Xie    D. F. Hong    Z. H. Xu    P. W. Liu    G. S. Yang 《Plant Breeding》2008,127(2):145-149

A recessive epistatic genic male sterility (REGMS) two‐type line, 9012AB, has been used for rapeseed hybrid seed production in China. The male sterility of 9012AB is controlled by two recessive duplicate sterile genes (ms1 and ms2) interacting with one recessive epistatic suppressor gene (esp). Homozygosity at the esp locus (espesp) suppresses the expression of the recessive male sterility trait in homozygous ms1ms1ms2 ms2 plants. In this study, we used a combination of bulked segregant analyses and amplified fragment length polymorphism (AFLP) to identify markers linked to the suppressor gene in a BC₁ population. From the survey of 1024 AFLP primer combinations, eight markers tightly linked to the target gene were identified. The two closest markers flanking both sides of Esp, P9M5₃₇₀ and S16M14₇₈₀, had a genetic distance of 1.4 cM and 2.1 cM, respectively. The AFLP fragment from P4M8₁₉₀, which co‐segregated with the target gene was converted into a sequence characterized amplified region marker. The availability of linked molecular markers will facilitate the utilization of REGMS in hybrid breeding in Brassica napus.  相似文献   

Improving hybrid rice parental lines for blast resistance by introgression of broad-spectrum resistance genes Pi54 and Pi9 by marker-assisted selection     

Thota Soujanya  Srinivas Prasad Madamsetty  Sandeep G  Vipparthi Hemalatha  Yamini K N  Edukondalu Bandela  Hari Prasad A S  Meenakshi Sundaram Raman  Revathi Ponnuswamy 《Plant Breeding》2023,142(3):300-311

Hybrid rice technology offers a great promise to produce 15% to 20% more yield than pure line varieties. The success of hybrid rice hinges on developing superior parental lines. To improve the blast resistance of hybrid rice parental line RP5933-1-19-2R, crosses were made with donors of two major blast resistance genes namely, Pi54 (Tetep) and Pi9 (IR71033–121-15) and the resulting F₁s were confirmed for their hybridity by using Pi54MAS and NMSMPi9-1 genic markers. The confirmed F₁s were intercrossed to obtain ICF₁s and selected positive plants by markers were backcrossed to the recurrent parent, as well as selfed for advancing further to BC₁F₃ and ICF₄ generations. The segregating plants were phenotyped for blast resistance at Uniform Blast Nursery. The identified complete restorers namely, RP 6619-1, RP 6616-26, RP 6619-3 and RP 6619-11 with Pi9 and Pi54 genes would serve as donors for broad spectrum blast resistance. This could ultimately lead to the development of new rice hybrids with improved resistance to blast disease, which is crucial for sustainable rice production and food security.  相似文献   

The transformation of the photo-thermo sensitive genic male-sterile line 261S of rice via an expression vector containing the anti-Waxy gene     

Rui Yang  Yongguo Zhou  Yueqin Cao  Zhongming Yin  Lijun Yang  Jianyue Li 《Breeding Science》2013,63(2):147-153

Transgenic photo-thermo sensitive genic male sterility Oryza sativa L. cv. “261S” plants with the anti-Waxy gene were successfully obtained using an Agrobacterium tumefaciens-mediated co-transformation method. Marker-free homozygous transgenic lines with the anti-Waxy gene were obtained. The setting seed rates of the transgenic plants via self-pollination or via crossing with the restorer line WX99075 rice and the 1000-grain weight of the transgenic plants and the F₂ hybrid seeds obtained by crossing the transgenic or non-transgenic plants with the restorer line WX99075 rice, and the number of panicles of the transgenic plants and yields of the F₂ hybrid rice, were analysed. Quality indexes of the transgenic plants and of the F₂ hybrid seeds were analysed. Our researches results indicate that hybrid female and hybrid descendant edibility could be improved via the introduction of the anti-Waxy gene, but the grain yields of the reserve seeds via self-pollination of the transgenic photo-thermo sensitive genic sterile lines and of the hybrid rice were not affected.  相似文献   

New insights into genotypic thermodependency of cytoplasmic male sterility for hybrid barley breeding          下载免费PDF全文

Timm Bernhard  Wolfgang Friedt  Rod J. Snowdon  Benjamin Wittkop 《Plant Breeding》2017,136(1):8-17

The cytoplasmic male sterility (CMS) system msm1 in barley is known to be thermosensitive, sometimes resulting in spontaneous fertility restoration in the absence of the corresponding restorer gene Rfm1. Here, we investigated genotypic differences concerning temperature sensitivity and the plant developmental stage at which elevated temperature induces spontaneous fertility restoration in three CMS mother lines. While one line stayed completely male sterile, a significantly higher fertility was observed in two lines after treatment from growth stage DC 41 until maturation. Microscopic analysis revealed that sterile anthers contained neither intact pollen, nor remains of aborted pollen grains, whereas pollen was visible in anthers of potentially fertile plants. We conclude that the barley CMS system affects anther and pollen development prior to meiosis. Elevated temperature during heading and flowering can lead to a spontaneous fertility restoration by reactivating pollen growth. Nevertheless, genotypic variation exists enabling the selection for stable CMS mother lines and the development of F₁ hybrids with high hybridity. As spontaneous fertility restoration due to environmental effects is difficult to phenotype, further investigations will focus on the development of molecular markers for marker‐assisted selection.  相似文献   

Identification of AFLP markers linked to one recessive genic male sterility gene in oilseed rape, Brassica napus     

L. P. Ke    Y. Q. Sun    D. F. Hong    P. W. Liu  G. S. Yang 《Plant Breeding》2005,124(4):367-370

The commercial utilization of heterosis in seed yield by means of hybrid varieties is of great importance for increasing oilseed rape production in China. This requires a functional system for the production of hybrid seed. The Brassica napus oilseed rape line 9012AB is a recessive epistatic genic male sterility (GMS) two‐type line, in which the sterility is controlled by two pairs of recessive duplicate sterile genes (ms1 and ms2) interacting with one pair of a recessive epistatic inhibitor gene (rf). Homozygosity at the rf locus (rfrf) inhibits the expression of the recessive male sterility trait in homozygous ms1ms1ms2ms2 plants. This study was conducted to identify molecular markers for one of the male fertility/sterility loci in the B. napus male sterility line 9012AB. Sterile bulk (BS) and fertile bulk (BF) DNA samples prepared from male sterile and male fertile plants of the homozygous two‐type line 9012AB were subjected to amplified fragment length polymorphic (AFLP) analysis. A total of 256 primer combinations were used and seven markers tightly linked to one recessive genic male sterile gene (ms) were identified. Among them, six fragments co‐segregated with the target gene in the tested population, and the other one had a genetic distance of 4.3 cM. The markers identified in this study will greatly enhance the utilization of recessive GMS for the production of hybrid seed in B. napus oilseed rape in China.  相似文献   

Pyramiding of thermosensitive genetic male sterility (TGMS) genes and identification of a candidate tms5 gene in rice     

Tamerlane Mark S. Nas  Darlene L. Sanchez  Genaleen Q. Diaz  Merlyn S. Mendioro  Sant S. Virmani 《Euphytica》2005,145(1-2):67-75

Three thermosensitive genetic male sterility (TGMS) genes – tms2, tgms and tms5 – were pyramided using linked microsatellite markers. Three TGMS donors, Norin PL 12 (tms2), SA2 (tgms) and DQ200047-21 (tms5) were utilized in generating crosses from which two-gene and three-gene pyramids possessing the RM11 allele of Norin PL 12, RM257 allele of SA2 and RM174 allele of DQ200047-21 were selected. All selected progenies were male-sterile at sterility-inducing conditions. In addition, rice SF21 was identified as a candidate tms5 gene because of its complete linkage with RM174. The 4,200-bp region was amplified from the TGMS line M105S and the two ends were sequenced. In silico analysis of partial nucleotide sequences showed that the region is similar to the SF21 pollen-specific gene of Arabidopsis and Helianthus. The M105S tms5 sequence was also compared to the SF21 sequence from the International Rice Genome Sequencing Project (IRGSP) database.  相似文献   

QTL analysis for cooking traits of super rice with a high‐density SNP genetic map and fine mapping of a novel boiled grain length locus          下载免费PDF全文

Jie Xu  Zhenyuan Shi  Weijun Ye  Liwen Wu  Dali Zeng  Zhenyu Gao  Longbiao Guo 《Plant Breeding》2015,134(5):535-541

Hybrid rice has contributed substantially to the improvement of grain production worldwide, yet its poor cooking and tasting characteristics have long been recognized. In this study, 132 recombinant inbred lines derived from LYPJ were used to identify quantitative trait loci (QTLs) for 12 cooking traits with the high‐density SNP linkage map recently developed by our team. We identified 17 QTLs on chromosomes 1, 2, 4, 5, 6, 7, 8, 9 and 11, which accounted for 7.50% to 23.50% of the phenotypic variations. A novel major QTL qBGL7 for boiled grain length was further fine‐mapped to an interval of 440 Kb between the two markers RM21906 and gl3 using a BC₃F₂ population. Two near‐isogenic lines with extreme boiled grain length, GX5‐176 and GX5‐101, could be directly used in improving cooking quality. We also identified a QTL for soaked grain width expansion rate, qSGWE6, in the Wx gene region on chromosome 6. The Wx differential regulation coincided with sequential variation between the two parents. Our work offered a theoretical basis for molecular breeding of high‐quality hybrid rice.  相似文献   

两用核不育系水稻叶绿体DNA的比较研究     

欧立军  李金龙  童梁  梁云浩  袁雅威 《中国农学通报》2010,26(11):50-53

以典型籼稻和粳稻作对照,对湖南应用较广的几个两用核不育系进行叶绿体DNA的ORF100、ORF29-TrnCGCA片段长度以及rps16基因内含子和TrnTUGU-TrnLUAA转录间区2个片段序列进行比较研究。结果表明,株1S、陆18S、株77S、H628S、88S、白天鹭S、G0543S、G03S、1103S和W6154S的叶绿体DNA的ORF100、ORF29-TrnCGCA两片段的大小均与典型的籼稻一致,其他材料则与典型粳稻相同;叶绿体DNA碱基多态性较丰富的2个区域rps16基因内含子和TrnT UGU -TrnL UAA间区的测序后发现4个籼粳稻特异位点,这4个位点对两用不育系叶绿体籼粳属性的判断与ORF100、ORF29-TrnCGCA片段的结果一致。根据育出组合的数量和质量,带有粳型血缘细胞质的两用核不育系如准S、810S、、中心S、C185S和培矮64S等显现了一定的优势。研究结果说明,通过籼粳稻杂交选育具粳稻血缘的籼型不育系是提高杂交水稻产量的有效途径之一。  相似文献   

Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp. pekinensis)     

Xiaoyong Xu  Xilu Sun  Yu Zhang  Lugang Zhang  Zhiyuan Fang 《Plant Breeding》2014,133(5):615-619

In this study, AFLP and SSR techniques were combined with the bulk segregant analysis (BSA) method to map the restorer gene BrRfp using an F₂‐segregating population comprising 258 individuals developed by crossing the polima (pol)‐like cytoplasmic male sterility (CMS) line 06J45 and the restorer line 01S325 of heading Chinese cabbage. A survey of 2048 AFLP primer pairs identified 21 polymorphic fragments, approximately half of which exhibited high similarity with the A09 chromosome sequence of Brassica rapa in the Brassica database (BRAD). Based on the genome sequence, three specific AFLP fragments linked with BrRfp were successfully converted into sequence‐characterized amplified region (SCAR) markers, named SC1233, SC2673 and SC2141. Subsequently, 178 pairs of SSR primers were redesigned for further screening, with five producing polymorphic amplification patterns. Linkage analysis showed that these markers were distributed along both sides of the BrRfp gene, with two markers, SSR03 and SSR2528, co‐segregating with the BrRfp locus in the F₂ population. These results may be valuable for marker‐assisted selection and map‐based cloning in heading Chinese cabbage.  相似文献   

水稻温敏核雄性不育系培矮64S广亲和位点S-5的SSR紧密连锁标记     

张向军  陈英之  韦燕萍  吕维莉  廖惠红  刘宇锋  杨新庆  李小勇  杨朗  李丽淑  李容柏 《分子植物育种》2006,4(4):506-512

广亲和(WC)是水稻(OryzasativaL.)亚种间杂种优势利用的重要遗传工具。本研究对我国南方大面积推广应用的亚种间两系杂交稻亲本培矮64S的WC基因S-5n进行分子标记定位。从培矮64S//T8/秋光三交F1中选出由263个高育和低育极端类型组成的标记群体,选用来源于CornellSSR连锁图的20个标记和根据GenBank数据库公布的序列合成的9个SSR标记,通过BSA(分离群体分析)法对标记群体进行分析,该S-5位点精确定位于第6染色体上,距SSR标记GXR6和RM276的距离只有0.2cM。培矮64S的S-5n对育性贡献可使小穗结实率从平均43.5%提高到77.5%,贡献率达34.0%,表明它是一个很强的控制广亲和特性的主效基因。本研究所获得的这些紧密连锁标记对分子标记辅助选择培育广亲和水稻品种和基因克隆具有重要的利用价值。  相似文献   

Inheritance and chromosome location of photoperiod‐thermo sensitive male sterility in wheat line Xinong 291S     

Puhui Dong  Yingang Hu  Guanghui Guo  Beiru He  Liming Wang  Jianguo Yuan 《Plant Breeding》2012,131(6):695-699

The present research aimed to study the inheritance and chromosomal location of a photoperiod‐thermo sensitive male sterility (PTSMS) gene in Xinong 291S (XN291S), which is a new PTSMS wheat line. The inheritance was studied in F₁ and F₂ populations derived from crosses between XN291S and eight wheat cultivars. All F₁ plants were fertile and the F₂ populations segregated in either 15 : 1 or 3 : 1 fertile : sterile ratios indicating that PTSMS was controlled by one or two recessive major genes. Five wheat cultivars carried homozygous dominant alleles for fertility, whereas the other three, including ‘Chinese Spring’ (CS), carried a single homozygous dominant allele. Chromosomal location of the PTSMS gene was studied by crossing a set of CS nulli‐tetrasomic lines to XN291S. Self‐fertility of F₁ hybrids XN291S/N5BT5A and XN291S/N5BT5D were significantly lower than the others. Therefore, the PTSMS gene in XN291S that differed from CS was located on chromosome 5B. Location of the second PTSMS gene needs further study.  相似文献   

Molecular mapping of the fertility-restoration gene Rf4 for WA-cytoplasmic male sterility in rice   总被引：4，自引：0，他引：4  

A. Ahmadikhah    G. I. Karlov 《Plant Breeding》2006,125(4):363-367

The wild abortive cytoplasmic male sterility (CMS‐WA) system, an ideal type of sporophytic CMS in indica rice, is used for the large‐scale commercial production of hybrid rice. Searching for restorer genes is a good approach when phenotyping is very time‐consuming and requires the determination of spikelet sterility in testcross progeny. To establish more precisely the genetical and physical maps of the Rf4 gene, high‐resolution mapping of this locus was carried out using simple sequence repeat (SSR) markers and newly designed markers in a F₂ population. The genetic linkage analysis indicated that five SSR markers (RM6737, RM304, RM171, RM5841 and RM228) on the long arm of chromosome 10 were linked with the Rf4 gene. Rf4 was flanked by two SSR markers RM171 and RM6737 at distances of 3.2 and 1.6 cM, respectively. Also, within the region between Rf4 gene and RM171, a newly designed primer pair, AB443, produced two sterile‐specific markers, AB443‐400 and AB443‐500, 0.5 and 1.03 cM from the gene. The flanking markers identified give promise for their application in molecular marker‐assisted selection (MAS) and they are also suitable for starting chromosome walking to clone Rf4 gene in the near future.  相似文献   

Genetics of thermosensitive genic male sterility in rice   总被引：4，自引：0，他引：4  

R. R. Borkakati  S. S. Virmani 《Euphytica》1996,88(1):1-7

Summary Inheritance of thermosensitive genic male sterility (TGMS) in Norin PL12 and IR32364TGMS and their allelic relationship were studied from F₁, F₂ testcross (TC) and F₃ generations of the crosses made with the two mutants and several fertile tester parents. F₂, TC and F₃ segregation behavior for pollen and spikelet fertility indicated that the TGMS trait in the two mutants was controlled by a single recessive gene. Allelic relationship studies indicated that TGMS genes of the two mutants were different. Since TGMS gene in Norin PL12 has been designated as tms ₂ , the TGMS gene present in IR32364TGMS is tentatively designated as tms ₃ (t) until allelic test is done with another TGMS gene (tms ₁ ) reported from China in a line 5460S seeds of which were not available.  相似文献