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1.
Various glues and adhesives were tested for their ability to remove the unabsorbed residue from drops of foliar applied radioactive nutrients to Ficus benjamina L. and Glycine max Merr. Most glues/adhesives adequately removed the unabsorbed residue from nutrients that dried forming a salt deposit on the leaf surface, but only 1.6–3.2% agar, Rhoplex 33, and Elmers Glue removed greater than 95% of the residue from nutrients that formed a moist hygroscopic residue on the leaf surface. Adding 25 mM KH2PO4 to 3.2% agar, followed by swabbing the treated area with lN HCl‐1% Liquinox detergent removed all unabsorbed residue from monovalent and divalent cationic an anionic nutrients including heavy metals. Methods to assay foliar absorption were compared by applying “Rb phosphate to leaves of intact plants, excised leaves and leaf discs. Excised leaves with their petiole or stem bases in water behaved similar to intact plants, whereas excised leaves or discs maintained in in a moist atmosphere behaved atypically due to slower and incomplete drying compared to intact plants.  相似文献   

2.
We examined the stability of plasmid RP4 and its expression of antibiotic-resistance genes in suspended and sorbed Pseudomonas putida in aquifer microcosms. Test tubes containing different proportions of sterilized aquifer soil and groundwater were inoculated with bacteria and incubated for up to 26 d. Serial dilutions were made to agar plates with or without antibiotics, to quantify the functional stability of the plasmid. The structural integrity of RP4 was examined by plasmid extraction, digestion with restriction enzymes, and agarose gel electrophoresis. The plasmid-borne resistance gene expression disappeared in 80-90% of the cells during day 1 of incubation in aquifer soil and then remained at that frequency throughout the experiment. The RP4 plasmid was present in cells without antibiotic-resistance gene expression, indicating that the observed loss of plasmid-encoded activity was most likely due to a reduction in expression of the resistance genes. The increased growth rate in groundwater amended with glucose and phosphate had no significant influence on plasmid loss or antibiotic-resistance expression, suggesting that plasmid loss and antibiotic-resistance expression were independent of the growth rate. Most of the reduction of resistance gene expression was associated with the presence of soil particles, and 70% of the resistance expression was retained in bacteria incubated for 1 d in groundwater alone. Bacteria sorbed to the soil particles had a lower frequency of expression of resistance genes than suspended bacteria, but the difference was not caused by sorbed inorganic or organic chemicals. Resistance gene expression was partly recovered in suspended bacteria after in vitro exposure to the antibiotics and after first isolating on agar without antibiotics and then replica plating to agar containing the antibiotics.  相似文献   

3.
Veterinary medicines enter agricultural soils by the use of animal excrements as fertilizers. To study their impact on soil bacterial communities, microcosms containing orthic luvisol soil were spiked with the antimicrobial agents sulfadiazine (SDZ) and chlorotetracycline (CTC) at three different concentrations (1, 10, 50 mg kg−1 soil) and incubated for 48 days at 20 °C. The impact on the microbial respiratory activity was measured continuously in a respirometer (Sapromat). Changes in bacterial community structure were visualized by means of PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rDNA derived from soil samples after 1, 7, 11 and 48 days. Additionally, growth inhibitory effects of SDZ and CTC on bacteria previously isolated from the same soil were tested in agar diffusion tests. In microcosms with soil and antibiotics only, no effects could be observed, either on respiratory activity or on bacterial population structure. Therefore, further incubations were conducted in the presence of an additional assimilable carbon source (5 g glucose kg−1 soil). In the presence of glucose, SDZ affected soil respiration as well as the bacterial community structure: Additional bands appeared and some bands already visible at the beginning of incubations increased in intensity. A clear relationship between SDZ concentrations and changes in DGGE patterns became visible. During 48 days of incubation, changes in DGGE patterns were minimal in microcosms with 50 mg SDZ kg−1soil indicating an inhibition of strains, which were capable of growing on glucose in the presence of lower SDZ concentrations. Only a few soil bacterial isolates (5 out of 47 strains tested) were weakly inhibited by SDZ in agar diffusion disk tests. Contrastingly, CTC inhibited growth of 12 soil bacterial isolates significantly in disk tests, but no effects on soil respiration and bacterial community structure could be observed. In the presence of the soil matrix the growth inhibitory potential of CTC decreased due to adsorption or complexation. This was confirmed in growth inhibition experiments with soil suspensions and time-dependent sampling.  相似文献   

4.
Sherlock MIS系统应用于土壤细菌鉴定的研究   总被引：17，自引：1，他引：17  
以10种已知菌株为例，对sherlock微生物鉴定系统（Sherlock MIS）的细菌鉴定准确性及培养基、活化时间和取样区域等因素对鉴定结果的影响进行了研究。结果表明该系统对TSBA培养基培养的细菌的鉴定准确率很高，除苏云金杆菌外其他菌株均鉴定到种的水平；但选用牛肉膏蛋白胨培养基培养细菌后，系统对供试菌株鉴定效果不理想。不同活化处理中所有样品的鉴定结果均正确，但以菌种活化2次后的SI值（相似指数）更高；取菌在二区或是三区则对鉴定结果影响不大。  相似文献   

5.
《Applied soil ecology》1999,11(1):79-90
Escherichia coli K12 strain (J5-3/RP4) persisted in sandy loam for more than 70 days when incubated at 10°C or 4°C. It decreased to below the level of detection within 20 days when incubated at 25°C. No loss of multi-resistance plasmid RP4 from the E. coli cells was detected during incubation in soil. There was a positive relation between the bacterial inoculum size and the following increase of the protozoan numbers in the soil. When soil microcosms were amended with an eukaryotic inhibitor, the period of survival was increased. These observations indicate a direct involvement of protozoa in the decline of E. coli in soil. Transfer of plasmid RP4 from E. coli donor bacteria to indigenous bacteria in soil was detected already 24 h after addition of the E. coli K12 donor strain. The efficiency of transfer during the first 48 h was approximately 10−6 transconjugants per donor. Inhibition of protozoan predation increased the number of transconjugants appearing in the soil, but the transfer efficiency per donor was not affected by the decreased predation. No transfer could be detected when the donor strain was washed and resuspended in saline before addition to the soil, but transconjugants were detected in this experiment when nutrients (LB) were supplemented after two days of incubation. Plasmid RP4 was maintained in the transconjugant soil bacteria throughout the experiment. The data presented here indicate that the indigenous bacteria in soil may serve as a sink for plasmidborne traits.  相似文献   

6.
液体培养研究不同土壤中硝化活性   总被引：2，自引：0，他引：2  
YUAN Fei  RAN Wei  SHEN Qi-Rong 《土壤圈》2005,15(3):379-385
A red soil, a fluvo-aquic soil and a permeable paddy soil were used in a long-term investigation to study changes in nitrification with treatments: 1) soil incubation, 2) liquid incubation inoculated with soil samples, and 3) liquid incubation inoculated with ammonia-oxidizing bacteria (AOB) from the soils. There were significant differences (P < 0.001) in nitrification rates among the three soils when measured for 28 days by adding (NH4)2SO4 at the rate of 154 mg N kg-1 dry soil to fresh soil. However, the amounts of nitrifying bacteria in the three soils were not related to soil nitrification capacity. When the soil samples or the isolates of AOB enriched from the corresponding soil were incubated in liquid with pH 5.8, 7.0 and 8.0 buffers and 10 mmol L-1 ammonium nitrogen, there were no significant nitrification differences in the same soil type at each pH. The ability to oxidize ammonia through AOB from different types of soils in a homogeneous culture medium was similar, and the soil nitrification capacity could reflect the inherent properties of a soil. Altering the culture medium pH of individual soil type also showed that acidification of an alkaline fluvo-aquic soil decreased nitrification capacity, whereas alkalinization of the acidic red soil and permeable paddy soil increased their nitrification. For a better insight into factors influencing soil nitrification processes, soil properties including texture and clay composition should be considered.  相似文献   

7.
Riparian wetlands are subject to nitrogen enrichment from upgradient agricultural and urban land uses and also from flooding by nitrogen-enriched surface waters. The effects of this N enrichment on wetland soil biogeochemistry may be mediated by both the presence of plants and the presence of redox-active compounds, specifically iron oxides in the soil. Despite the extensive research on wetland N cycling, the relative importance of these two factors on nitrogen is poorly known, especially for forested wetlands. This study evaluates the responses of the N and the Fe cycles to N enrichment in a riparian forested wetland, contrasting vegetated field plots with plots where the vegetation was removed to test the role of plants. Furthermore, in vitro anaerobic incubations of the experimental soils were performed to track Fe chemical changes over time under anoxic or flooded conditions. Wetland soils treated with N in form of urea, as expected, had significantly higher amounts inorganic nitrogen. In the soils where vegetation was also removed, in addition to inorganic nitrogen pool, increase in organic nitrogen pool was also observed. The results demonstrate the role of vegetation in limiting the effects excess urea has on different soil nitrogen pools. Results from anaerobic incubation of the experimental soils demonstrated the effects of N enrichment on the wetland Fe cycle. The effects of excess nitrogen and the role of vegetation on the Fe cycle in riparian wetland soil became more evident during anaerobic incubation experiments. At the end of the field experiment, Fe concentrations in the soils under the treatments were not significantly different from the control soils at the 5% confidence level. However, during the anaerobic incubation experiment of soils collected at the end of the experiment from these plots, the N-enriched soils and the unvegetated soils maintained significantly elevated concentrations of reducible Fe(III) for the initial 2-week period of incubation, and the soils collected from the plots with both the treatments had the highest Fe(III) concentrations. After 20 days of incubation, however, the Fe(III) concentrations decreased to the similar concentrations in all the incubated soils. The study clarifies the roles vegetation play in mediating the effects of N enrichment and also demonstrates that N enrichment does affect wetland redox cycle, which has strong implications on ecosystem services such as water quality improvement.  相似文献   

8.
Natural aggregates of topsoil samples of six British soils (Hanslope, Ragdale, Evesham, Denchworth, Flint and Salop) were incubated with added nutrients and changes in their stability to wet sieving determined. Under anaerobic incubation the type of nutrient added had little effect on stability. Aerobic incubation with glucose gave a larger proportion of stable aggregates than with water. With peptone stability usually decreased, and occasionally increased, which may indicate differences in the binding mechanisms of the soil particles. Both sterile and unsterile aggregates incubated with water became more stable than controls; the rapidity of this change suggested a physical, rather than microbiological cause. Very stable grassland aggregates changed little on aerobic incubation with water, but became more stable with solutions of glucose or peptone. Artificial aggregates made from the same soil developed considerable stability to water on aerobic incubation, but remained almost totally unstable when incubated anaerobically.  相似文献   

9.
The oxidizing power of rice roots was observed in narrow transparent root boxes containing different media. Plants precultivated in nutrient solution were embedded in semisolid agar medium to observe oxidation of ferrous iron cations and leuco methylene blue as well as solubilization of ferrous sulfide. In the presence of ferrous sulfate reddish brown coloration due to formation of ferric oxide/hydroxide was observed around the roots and on the root surface during one day of incubation. When agar medium blackened by ferrous sulfide was used, the root zone became transparent. Within a few hours leuco methylene blue was oxidized to methylene blue on and near the roots. Furthermore, seedlings were grown in agar medium containing ferrous sulfide inoculated with soil filtrate. Besides diffuse ferric iron precipitation, iron was also deposited on spherically shaped structures in the rhizosphere and near the agar surface as well as in slimy layers appearing on the root surface. The spherical structures and slimy layers were obviously bacterial colonies extending with time. As the roots grew old, parts of them turned black. In the rhizosphere, black spots occurred resembling colonies of sulfate-reducing bacteria. Rice was also grown in sand supplemented with nutrients and iron sulfide. While root growth was straight in agar, it was twisted in the sand medium. Again, heavy ferric iron deposition occurred on the root surface. On older root parts the lateral roots became blackish. The results suggest participation of bacteria in ferric iron deposition in the rhizosphere of rice.  相似文献   

10.
Factors influencing C2H4 production in a silt loam were investigated in an effort to determine the source of this gas in soil. Air-dried samples of soil in glass vials were moistened to about ?10 kPa, sealed with rubber septa, and incubated at 30 or 35°C with an original atmosphere of air or O2-free N2. C2H4 concentrations in the vials were determined by gas chromatography.Addition of the antibacterial agents chloramphenicol or novobiocin to the soil inhibited C2H4 production, whereas the antifungal agent cycloheximide had no effect. Sodium azide and sodium cyanide also reduced C2H4 production. Treatment of the soil with moist heat (i.e. passing a steam-air mixture through it) at 80°C for 30 min failed to reduce the ability of the soil to produce C2H4 during subsequent incubation at 30°C, but autoclaving it twice at 121°C prevented C2H4 production. As with nonheated soil, C2H4 production from soil treated at 80°C was prevented by novobiocin but not by cycloheximide. Only about 10% of the bacteria isolated from nontreated soil were spore-formers. In contrast, 95–98% and possibly more of the bacteria isolated from heat-treated soil were spore-formers, including those in soil which was heat-treated and then incubated moist at 30°C for an additional 3 days before dilution plating. Addition of methionine had no effect on the production of C2H4 in anaerobic soil, whereas ethionine, chlorogenic acid, and ethylenediaminetetraacetic acid (EDTA) all enhanced C2H4 production. Ethionine, but not chlorogenic acid or EDTA, also resulted in considerable C2H4 accumulation in autoclaved soil; the C2H4 detected in ethionine-amended soil was apparently nonmicrobial in origin. Soil samples incubated at constant temperatures of 30, 50, or 70°C all produced C2H4.The results collectively indicate that C2H4 in soil is most likely produced by facultative or strictly anaerobic bacteria which are probably spore-formers and may also be thermophilic. Several isolates of spore-forming bacteria were inoculated into autoclaved soil, but none produced appreciable amounts of C2H4 under the test conditions.  相似文献   

11.
《Applied soil ecology》2001,16(1):85-90
One DDT-contaminated soil and two uncontaminated soils were used to enumerate DDT-resistant microbes (bacteria, actinomycetes and fungi) by using soil dilution agar plates in media either with 150 μg DDT ml−1 or without DDT at different temperatures (25, 37 and 55°C). Microbial populations in this study were significantly (p<0.001) affected by DDT in the growth medium. However, the numbers of microbes in long-term contaminated and uncontaminated soils were similar, presumably indicating that DDT-resistant microbes had developed over a long time exposure. The tolerance of isolated soil microbes to DDT varied in the order fungi>actinomycetes>bacteria. Bacteria from contaminated soil were more resistant to DDT than bacteria from uncontaminated soils. Microbes isolated at different temperatures also demonstrated varying degrees of DDT resistance. For example, bacteria and actinomycetes isolated at all incubation temperatures were sensitive to DDT. Conversely fungi isolated at all temperatures were unaffected by DDT.  相似文献   

12.
Abstract

Most soil testing laboratories require soil samples collected for the pre‐sidedress soil nitrate test (PSNT) to be dried before shipment. Shipment of field‐moist samples would make it easier to use the test. The objective of this study was to measure the effect of bag type on changes in soil nitrate in samples collected for the PSNT. Soil samples were collected from the surface foot of soil when corn (Zea mays L.) plants were 15‐ to 30‐cm tall. Four fields with a history of manure applications were sampled. The bulk sample was subsampled in the field and the subsamples were kept cool during transport to the laboratory and were immediately air dried after arrival at the laboratory. Field‐moist subsamples from each bulk sample were placed into either cloth bags or plastic‐lined paper bags after arrival at the laboratory. Four replications of the subsamples were incubated at 30°C for 1, 2, 3, and 4 days. After removal from the incubator, the subsamples were immediately spread to air dry. The soils incubated in the plastic‐lined paper bags did not significantly increase in nitrate after one day of incubation. There was a significant increase, however, in nitrate each day after the second, third and fourth day of incubation. The soils incubated in the cloth bags did not significantly increase in nitrate until the fourth day of incubation. The average increase in nitrate‐N concentration in the cloth bags between day 3 and day 4 was 1.5 mg kg L‐1soil. The results suggest that cloth bags could be used to ship field‐moist soil samples for the PSNT without significant changes in soil nitrate concentrations.  相似文献   

13.
The effects of some pesticides upon substrate colonization by Trichoderma harzianum Rifai in the presence of other soil fungi.A herbicide (propyzamide) and 5 fungicides (benomyl, quintozene, vinclozolin. thiram, prothiocarb) were added at various concentrations to two Trichoderma harziunum-enriched garden soils. After thorough mixing, followed by solidifying with water agar, discs of these mixtures were cut with a cork-borer and transferred to a non specific nutrient medium (PDA). The development of T. harzianum on the agar, around each implant, was estimated after incubation for 1 week at 28°C.In the presence of other soil fungi, T. harzianum development was scarcely or not at all modified by prothiocarb, propyzamide and quintozene. It was highly depressed by benomyl. Vinclozolin was only slightly inhibitory. In our experimental conditions, T. harzianum development was strongly enhanced by thiram, even at a high dosage (300 μg ml?1).The limits and the advantages of the method are estimated and the results are discussed in view of practical applications.  相似文献   

14.
Two different soils, either fresh, or dried and remoistened, were incubated with 1% labelled glucose for 28 days at about 20° or 5 °C and the transformation of the glucose to other sugars measured. Xylose formed a much greater proportion of the sugars synthesized at low temperature in pre-dried soil than in fresh soil, or at 20 °C in pre-dried or fresh soil. Numbers of bacteria, fungi (including actinomycetes) and yeasts were determined for each type of incubation. Because the combination of conditions leading to xylose synthesis by yeasts in predried soil are unlikely in the natural state it is concluded that most soil xylose originates in the residues of plants.  相似文献   

15.
The sensitivity of soil bacteria towards Pb was investigated. Soil suspensions from fourteen different soil types with a high or low Pb content were plated out on soil extract agar containing various concentrations of PbCl2.In agar with a high Pb content, higher bacterial counts were found with suspensions from Pb-containing soils than with those of soils with a low Pb content. In the Pb-containing soils, proportionally more gram-negative rods were present while coryneform bacteria decreased. In an additional experiment, in which Pb was added to a sandy soil, more Pb-tolerant bacterial strains were found 3 years later than in the same soil without Pb.When pure cultures of the bacteria isolated from the soils were tested in liquid media for Pb tolerance, a higher proportion of tolerant strains was found in Pb-containing soils. Among strains of gram-negative bacteria isolated from these soils a higher proportion of tolerant strains was found than in corneform bacteria.It was concluded that as a consequence of Pb pollution of soil a selection of Pb-tolerant bacteria may take place.  相似文献   

16.
生物质炭是具有高度热稳定性和较强吸附特性的含碳物质,不同来源生物质炭的结构性质可能存在着很大的差异。为此对2种自制的不同来源生物质炭和1种商业黑炭进行了结构表征,并研究了添加生物质炭对土壤有效养分和腐殖质组成的影响。结果表明,不同来源的生物质炭在结构上有明显区别：秸秆生物质炭的芳构化程度和热稳定性最低,脂族性最强;商业黑炭缩合程度和热稳定性最高,脂族性最弱;松枝生物质炭介于二者之间。向土壤中添加秸秆生物质炭和松枝生物质炭培养45d后,土壤有机碳含量、胡敏酸和富里酸含量、有效养分含量都有不同程度的增加,同时胡敏酸的色调系数ΔlgK降低,对土壤有机碳的长期保存有积极意义。  相似文献   

17.
Abstract

The effects of irrigating with saline water on native soil fertility and nutrient relationships are not well understood. In a laboratory experiment, we determined the extent of indigenous nutrient [calcium (Ca), magnesium (Mg), potassium (K), manganese (Mn), and zinc (Zn)] release in salt-saturated soils. Soils were saturated with 0, 75, and 150 mmolc L?1 sodium chloride (NaCl) solution and incubated for 1, 5, 10, and 15 days. The saturation extracts were analyzed for pH, ECe, and water‐soluble Ca, Mg, K, Mn, and Zn, and the remainder soil samples were analyzed for exchangeable forms of these elements. In a subexperiment, three soil types (masa, red‐yellow, and andosol) were saturated individually either with 100 mmolc L?1 of NaCl, sodium nitrate (NaNO3), or sodium sulfate (Na2SO4) salt. These salts were also compared for nutrient release. Soils treated with NaCl released higher amounts of water‐soluble than exchangeable nutrients. Except for Zn, the average concentrations of these nutrients in the soil solution increased significantly with time of incubation, but concentrations of the exchangeable forms varied inversely with time of incubation. The masa soil exhibited the highest concentrations of Ca and Mg, whereas K was highest in andosol. The extract from soils treated with NaCl contained greater amounts of soluble cations, whereas soils treated with Na2SO4 produced the lowest concentration of these elements irrespective of the type of soil used.  相似文献   

18.
The objective of this work was to study the effect of different biochar on alkaline calcareous soil, inherently low in soil organic carbon and fertility. Experiments were conducted in laboratory and greenhouse. Biochar was produced from wheat and rice straws at pyrolysis temperatures of 300°C, 400°C and 500°C (denoted as WSB300, WSB400, WSB500, RSB300, RSB400 and RSB500, respectively). In the first experiment, soil was incubated with biochar (1.0 % w/w) for up to 50 weeks. The results indicate that, WSB300 caused a significant decrease in soil pH and increased the CEC and nutrients (N, P and K) after 50 weeks of incubation. In the second experiment, maize plants were grown in pots containing calcareous soil amended with WSB and RSB for 60 days the results revealed that the application of WSB300 caused a significant increase in shoot (36%) and root (38%) dry matters over the respective control. Moreover, the highest nutrient concentrations (N and P) in shoot and root were observed with the WSB300 compared to other treatments. Therefore, it is concluded that application of wheat straw biochar produced at low temperature (WSB300) could be successfully used to improve soil properties and growth of plants in calcareous soils.  相似文献   

19.
Abstract

Aerobic incubation methods have been widely used to assess soil nitrogen (N) mineralization, but standardized protocols are lacking. A single silt loam soil (Catlin silt loam; fine‐silty, mixed, superactive, mesic, Oxyaquic Arguidoll) was subjected to aerobic incubation at six USDA‐ARS locations using a standardized protocol. Incubations were conducted at multiple temperatures, which were combined based on degree days (DD). Soil water was maintained at 60% water‐filled pore space (WFPS; constant) or allowed to fluctuate between 60 and 30% WFPS (cycle). Soil subsamples were removed periodically and extracted in 2 M potassium chloride (KCl); nitrate (NO3) and ammonium (NH4) concentrations in extracts were determined colorimetrically. For each location, the rate of soil organic‐matter N (SOMN) mineralization was estimated by regressing soil inorganic N (Ni) concentration on DD, using a linear (zero‐order) model. When all data were included, the mineralization rate from four datasets was not statistically different, with a rate equivalent to 0.5 mg N kg?1 soil day?1. Soil incubated at two locations exhibited significantly higher SOMN mineralization rates. To assess whether this may have been due to pre‐incubation conditions, time‐zero data were excluded and regression analysis was conducted again. Using this data subset, SOMN mineralization from five (of six) datasets was not significantly different. Fluctuating soil water reduced N‐mineralization rate at two (of four) locations by an average of 50%; fluctuating soil water content also substantially increased variability. This composite dataset demonstrates that standardization of aerobic incubation methodology is possible.  相似文献   

20.
通过全灭菌环境下的土壤五氯酚（PCP）污染模拟实验,分别测定水提取态、乙醇提取态、腐殖质固定态以及蚯蚓生物累积态PCP的变化,研究添加两种不同蚯蚓品种（赤子爱胜蚓和壮尾环毛蚓）和堆肥对土壤PCP老化过程的影响。结果显示,在42d的培养期内,水提取态PCP在全灭菌土壤对照和添加灭菌堆肥的处理中随时间推移逐渐下降,存在明显的老化效应。在蚯蚓处理中,水提取态PCP呈现先下降后上升的趋势,说明蚯蚓促进土壤PCP的解吸附,这主要与蚯蚓的生物扰动作用以及调节土壤pH趋向中性的作用直接相关。培养结束时,蚯蚓能够有效促进土壤PCP向腐殖质固定态转化,并进一步促进PCP的去除,这与蚯蚓提高土壤腐殖质的含量,促进PCP的化学还原转化作用以及蚯蚓自身对PCP的消解作用有关。壮尾环毛蚓比赤子爱胜蚓更能有效促进PCP的转化与去除,这与两者的生活习性不同有关。灭菌堆肥对全灭菌土壤PCP向其他形态转化的影响不显著。  相似文献   

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