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以印楝茎尖为外植体进行离体快速繁殖，外植体采用次氯酸钠预处理，用0．1％的HgCl2浸泡消毒，共设66个处理，3次重复，分3次试验，结果表明，芽增殖培养基最佳组合为MS＋6－BA 7．0g/L IAA 0.01mg/L ZT 0.1mg/L，生根培养基为1／2MS＋NAA0．5mg/L，能有效促进芽生根，且移栽成活率可达80％以上。 相似文献
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以ＭＳ为基本培养基，取苹果枣萌蘖苗茎段作外植体进行离体快繁研究，筛选出最佳分化培养基的激素配比是６－ＢＡ６．０ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ，生根培养基ＩＢＡ用量以０．６ｍｇ／Ｌ较好。试管苗繁殖系数达６．２，生根率达９１．６％，移栽成活度达９８％。 相似文献
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樱桃矮化砧木SD——13组培快繁技术研究 总被引：8，自引：0，他引：8
利用ＳＤ－１３樱桃矮化砧木的嫩茎梢作为外植体，进行离体培养，研究出其生长分化的基本培养其为ＭＳ，最佳分化培养基为ＭＳ＋ＢＡ０．６ｍｇ／Ｌ＋ＮＡＡ０．０５ｍｇ／Ｌ，分化率达９４％，最佳生根培养基为１／２ＭＳ＋ＮＡＡ０．０４ｍｇ／Ｌ，生根率达９２％，移栽成活率达９５％。 相似文献
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为探究可溶性糖含量在雷公藤组织培养过程中发挥的效用。采用蒽酮法对雷公藤组织培养各阶段的可溶性糖含量进行测定。结果表明：14 d为雷公藤外植体生长发育一个小周期，蔗糖是生根培养阶段最佳碳源，30 g·L-1为最佳添加浓度，生根率达100%。可溶性糖含量在生长发育的0-7 d、14 d-21 d呈上升趋势，而其他时间段含量下降，说明可溶性糖是一种重要的能量物质。不同碳源处理中，蔗糖是最佳碳源，而葡萄糖不仅会造成雷公藤外植体生根效果差，生长发育还受到抑制，外植体处于营养缺乏状态。在雷公藤组织培养过程中，可通过外源蔗糖的添加以促进雷公藤外植体的生长发育。 相似文献
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切花品种非洲菊的组培和快繁 总被引：1，自引：0，他引：1
以非洲菊幼叶及幼嫩花托为外植体，采用两次灭菌方法，用MS作基本难关基，添加不同浓度的6－BA，KT，NAA，IBA等进行诱导分化，增殖，生根培养和过渡炼苗，不同品种的诱导率差异较大，采用MS＋6－BA2．0－5．0mg/L(单位下同）＋NAA0．2－0．5培养基，愈伤组织诱导率在80％以上，采用MS＋6－BA0．5－1．0＋NAA0．1－0．2培养基对芽的生长及增殖效果较好；生根采用MS或1／2MS＋NAA0．2－0．5或IBA0．2均可，过渡移栽在蛭石：珍珠岩：火土灰＝1：1：1中，成活率可达90％以上。 相似文献
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欧洲大樱桃试管快繁技术研究 总被引：1，自引：0，他引：1
取欧洲大樱桃茎段作外植体，探讨了其快速繁殖技术，筛选出最佳增殖、生根培养基，找出了影响试管苗移栽成活率的因素。试管苗增殖系数达到10左右，生根率达92.5%，生根率达92.5%，移栽成活率达95％以上。 相似文献
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Endogenous polyamine concentrations in apical buds of peach (Prunus persica (L.) Batsch cv. 'Hakuho') and monterey pine (Pinus radiata D. Don) seedlings, saplings and mature trees were analyzed by high performance liquid chromatography coupled with fluorescence detection to establish physiological correspondences and disparities between the species. Although there were many differences in polyamine metabolism between peach and monterey pine, several similarities were found. Specifically, in both species, the ratio between concentrations of free polyamines and low molecular-weight polyamine conjugates was greater than and less than unity in juvenile and adult plants, respectively. Free putrescine concentration decreased with age in both species. 相似文献
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核果类果树中microRNAs的生物信息学预测及验证 总被引：2，自引：0，他引：2
microRNA(miRNAs)是一类非编码小分子RNA,在植物生长发育、新陈代谢以及逆境生理中起重要作用。基于生物信息学的基因搜索和同源搜索,从NCBI数据库中登录的核果类果树的EST和GSS中寻找miRNAs。从核果类果树的107982条ESTs和48273条GSSs中搜寻到了11条miRNAs前体序列,编码11条成熟体序列,并预测到19个靶基因,分别编码与生长发育、新陈代谢和转录调节等相关的蛋白。利用miRbase数据库进一步分析表明:11条miRNAs属于8个microRNA家族,分别来自桃和梅,其大小为20～23bp,其中6条为21bp,而且ppe-miR162a和ppe-miR164f保守性最高。采用poly(A)RT-PCR方法随机对预测的ppe-miR156h,pmu-miR482,ppe-miR399a,ppe-miR171a进行组织表达验证,发现ppe-miR156h在果梅花芽中表达,pmu-miR482,ppe-miR399a,ppe-miR171a在桃叶片中表达。 相似文献
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为给调控观赏桃花花期提供理论依据,研究了不同质量浓度的水杨酸(SA)在开花过程中对照手桃Prunus persica‘Terutemomo’开花的影响。在照手桃花前期及花中期进行SA喷施,测定其花芽、花蕾及枝条的可溶性糖含量、可溶性蛋白含量、C/N值及内源激素(GA、IAA、ZR、ABA)含量的动态变化。结果表明:2个时期SA各质量浓度处理使‘照手桃’的始花期推迟1~2 d,增强了开花过程中能量的储存,加快了可溶性蛋白的分解,增强了新陈代谢和激素活动,对开花有利。 相似文献
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Prunus ferganensis (Kost. & Riab) Kov. & Kost, a close relative of cultivated peach (Prunus persica (L.) Batsch.), is native to arid regions of central Asia. A distinguishing feature of P. ferganensis is its prominent, elongated, unbranched pattern of leaf venation. To determine whether the long-vein trait could be used as a marker in breeding for drought tolerance, we investigated the association between this trait and the leaf morphological and physiological parameters related to drought response in P. ferganensis, P. persica and two interspecific hybrids, one with the long-vein trait (BY94P7585) and one without (BY94P7589). The four genotypes were grafted onto "Guardian" peach rootstock and half of the plants were assigned to a drought treatment in which irrigation was limited to 25-50% of the evapotranspiration (ET) rate measured in the remaining well-watered plants, which were irrigated to runoff daily. The drought treatment reduced photosynthesis and leaf conductance by 49-83% and reduced total leaf area per plant by 17-24%, but generally did not affect mid-morning leaf water potential. Leaf gas exchange did not differ among genotypes in either treatment. Sorbitol accumulated in mature leaves in response to drought, but neither its amount nor its metabolism varied systematically with climatic adaptation among genotypes. Accumulation of transport sugars was highest in P. ferganensis, indicating that growth reduction may represent an important strategy for coping with drought in this species. Prunus ferganensis and the hybrids had higher ET than P. persica, and seemed to use water opportunistically, maintaining high gas exchange rates and consequently high ET when water was available, and avoiding low water potentials through stomatal closure as soil water declined. Leaf size (cm(2) leaf(-1)) and specific leaf area (cm(2) g(-1) dry mass) were lower in P. ferganensis and the hybrids than in P. persica. We conclude that the long-vein trait is not a reliable marker for drought tolerance, but leaf traits of P. ferganensis such as size and specific leaf area may be useful in P. persica breeding programs targeting drought tolerance. 相似文献
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为了研究大气CO_2浓度升高对西藏特有植物的影响,以野生光核桃为研究材料,以栽培桃为对照,以高浓度CO_2为胁迫条件,分析野生光核桃和栽培桃的叶绿素含量、酶活性、叶片呼吸与光合指标的变化特征。结果表明:在高浓度CO_2条件下,栽培桃植株的部分叶片有黄化趋势,野生光核桃变化不明显。在高浓度CO_2条件下,野生光核桃抗氧化酶、光合和呼吸等指标均做出了积极的响应,保持了较高的酶活性,同时保持了正常的电子传递效率,且光合效率的下降主要是由非气孔因素引起。因此,与栽培桃相比,野生光核桃对高浓度CO_2逆境的抗性更强。 相似文献
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12个桃品种的花芽休眠需冷量和开花需热量 总被引：3，自引：0，他引：3
在浙西南地区研究12个桃品种花芽休眠需冷量和开花需热量生态指标.犹他模型测定结果显示:12个桃品种中,花芽休眠需冷量低的品种为春蜜、超红、中油11号、中油7号、中油4号,约为550～650 cu;其次是丽油5号、仓方早生、燕红、丽油3号、早凤凰,约为650～ 800 cu;需冷量高的品种为新川中岛和赤月,约为900～ 950cu.同一地区不同品种需热量差异较大,同一品种年际间差异相对较小.以生长度天模型试验结果表明:开花需热量低的品种为赤月和丽油3号,大约400 ～ 500 d·℃;其次是新川中岛,大约500～600 d·℃;春蜜、丽油5号、早凤凰、中油4号、中油7号、仓方早生和燕红大约600 ～ 700 d·℃;需热量较高的是中油11号和超红,约为700～800 d·℃.12个桃品种花芽休眠需冷量与开花需热量之间呈乘幂函数曲线显著负相关. 相似文献
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紫叶桃是重要的园林彩叶树种,叶色美丽,观赏期长,具有很高的园林应用价值。以紫叶桃的叶片和嫩茎段为外植体,用不同浓度生长调节物质进行愈伤组织诱导试验,建立紫叶桃的再生体系。结果表明,诱导愈伤组织时,MS＋2mg／LBA＋1mg／L2,4-D对叶片诱导效果最佳,其诱导率达80％;MS＋1mg／LBA＋1mg／L2,4-D对茎段诱导效果最佳,其诱导率达100％。在MS＋1mg／LBA＋1mg／L NAA培养基上愈伤组织能直接分化出不定芽。 相似文献