共查询到18条相似文献,搜索用时 187 毫秒
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旱地冬小麦籽粒充实期间光合产物的分配与运转 总被引:1,自引:0,他引:1
^14示踪试验表明,在大田自然干旱、浇水和浇水+追踪N肥3种处理条件下,冬小麦植株^14C光合产物对籽粒的比率分别为85.9%,78.0%和73.4%;旗叶节以上器官--旗叶、旗叶鞘、旗叶节间、芒及颖壳5种光合器官的^14C产的运至籽粒的比率分别为59.9%,54.2%和44.9%。 相似文献
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油菜(B.napus)属种间杂种后代的遗传研究 总被引:4,自引:0,他引:4
潘大仁 《农业生物技术学报》1999,7(2):141-146
本研究通过油菜与萝卜属间杂种(ACR)为亲本材料,分别与芸苔Bcampestris和甘蓝B.oleracea杂交,属种间杂种(AACR,ACCR)再与甘蓝型油菜品种杂交,以获得稳定的转基因属种间杂种材料。结果指出,属间杂种与芸苔B.campestris杂交比与及甘蓝B.oleracea杂交更易获得后代。这些杂种后代分别与甘蓝型油菜回交也表现了这种趋势。细胞学分析指出,属种间杂交由于异源染体的存在 相似文献
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长豇豆叶片^14C光合产物运转与分配的研究 总被引:6,自引:0,他引:6
试验以两个蔓生长豇豆品种为材料,在不同时期不同叶位喂饲^14CO2,研究长豇豆叶片^14C光合产物的运转与分配规律。结果表明,长豇豆营养生长期,叶片的光合产物输出率较低(54.85%),生殖生长期,上,中,下叶位叶片的光合产物输出率均较高,平均为88.74%,且随着叶位的上升而增大。营养生长期,叶片的光合产物向上、下部器官运转,其中95%以上运转到茎叶,且运转到上部茎叶的光合产物稍多,根只获得少量 相似文献
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土壤薄板层析测定苯并(α)芘(BaP)的Rf值小于0.1,在土壤中BaP属于不迁移性有机化合物,土壤中BaP被降解成极性化合物(占总^14C-BaP23.5%)水溶形式(占1.7%)和非极性形式(占38.4%)。土壤^14-Bap组合态占36.4%。土壤表层(0~1cm)^14C-BaP为0.1%,水稻苗期对BaP吸收速度快,且数量大,拔节期间吸收转缓,分蘖期后吸收达平衡。水稻体内^14C-BaP 相似文献
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本研究克隆了萤光假单胞菌AS1.55(Pseudomonas fluorescensAS1.55)的亮氨酸基因(leu^+)EcoRI片段(-6.6kb),并获得含有该片段的重组质粒pBR322-LEU。从pBR322-LEU质粒中分离出leu^+EcoRI片段,将其插入到nifA质pMC71A的EcoRⅠ位点使氯霉素抗性基因失活,从而构建了不带抗药性基因nifA质粒pMC71A-LEU。 相似文献
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利用~(14)C示踪法研究杉木光合产物分配和杉木根系分泌物 总被引:2,自引:0,他引:2
用 ̄(14)C示踪法研究了杉木光合产物的分配和杉木根系的分泌物。结果表明,杉木饲喂 ̄(14)CO2后1天,光合产物大部分滞留在叶中,只有27.38%从叶中输送到新梢、主枝、根和侧枝中,标记后3天,光合产物分配率仍以叶为最高,但所占百分比下降,而主枝、新梢、根和侧枝的分配率相应地有了增加,至标记后22天,光合产物的分配率以新梢为最高,其次为根,叶退居其后。杉木光合产物输送到根部后,可很快转化为分泌物并排出体外,其分泌量在标记后第4天出现峰值,此后 ̄(14)C分泌物量不断下降。杉木根系分泌物中的有机小分子物质,以糖类为最多,其次是有机酸,氨基酸居第三。 相似文献
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黄河流域水土保持工作的新发展黄自强(水利部黄河水利委员会,郑州450003)NewAdvanceofSoilandWaterConservationWorkintheYellowRiverBasin¥HuangZiqiang(YellowRiverC... 相似文献
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固氮粪产碱菌中nif,ntr和gln基因鉴定及nifA克隆 总被引:4,自引:1,他引:3
本工作采用^32P标记的与固氮(nif)和一般氮代谢(ntr和gln)系统有关的基因探讨,对不同限制性内切酶完全酶解的粪产碱菌Alcaligenes faecalis总DNA进行Southern杂交分析,证明在粪产碱菌中存在与nifH、nifDK、ntrA,ntrC、glnB、glnD同源的DNA序列。以Azospirllum brasilense Sp7 nifA DNA为探针,经三轮杂交筛选。 相似文献
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It has been reported that nitrogen in the leaves of tobacco plant was translocated to the stem and root during the ripening period (1). Free amino acids in the leaves would play an important role in the translocation of nitrogen. It was also reported that the amino acids injected into the tobacco leaves were translocated to the roots (2,3). It seems probable that the translocation of amino acids would be characteristic among the individual amino acid. As reported previously, 14C assimilated in the leaves is translocated rapidly to the roots (2). Although it has been well known that the photosynthetic product was translocated mainly in the form of sugar, possibility that a part of photosynthetic products is translocated in the form of amino acid would be expected, because the carbon is incorporated rapidly into some of the amino acids, such as glycine, serine and alanine (4). 相似文献
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本试验选用5个性状各有特色的小麦优异突变体进行双列杂交(无反交)。利用10个组合F1和5个亲本材料研究了8个农艺性状的杂种优势和配合力。F1显性程度表明:除抽穗期以负向优势为主外,其余7个性状均以正向优势占主导。亲本本身表现与一般配合力效应大小呈显相关,但一般配合力效应大小与杂种优势的表现并不完全一致。作为种质资源,矮秆突变体890376对降低后代株高最有利,大粒突变体核生二号最有利于提高后代的 相似文献
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超级杂交稻光合同化物的运转分配特性 总被引:1,自引:1,他引:0
采用盆栽法和14C-同位素示踪技术,以杂交稻组合汕优46为对照,研究了超级杂交稻组合"两优293"、"准两优527"的光合能力和光合同化物运转分配特性。结果表明,在不同生育期,两个超级杂交稻组合与对照组合汕优46相比,其单株同化14CO2的总量均显著高于对照,达到对照的1.49~1.96倍;14C-光合同化物最终分配至穗中的比例也较对照高,且其14C-光合同化物运输至穗中的速率也比对照快。表明超级杂交稻组合"两优293"、"准两优527"具有较强的光合同化物生产和运输能力。 相似文献
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J. Lage B. Skovmand R. J. Peña S. B. Andersen 《Genetic Resources and Crop Evolution》2006,53(5):955-962
The wild diploid goat grass (Aegilops tauschii Cosson), and the cultivated tetraploid emmer wheat (Triticum turgidum L. subsp. dicoccon (Schrank) Thell.) may be important sources of genetic diversity for improving hexaploid bread wheat (Triticum aestivum L.). Through interspecific hybridization of emmer wheat and Ae. tauschii, followed by chromosome doubling, it is possible to produce homozygous synthetic hexaploid wheat. Fifty-eight such synthetic
hexaploids were evaluated for grain quality parameters: grain weight, length, and plumpness, grain hardness, total protein
content, and protein quality (SDS-Sedimentation volume, SDS-S). Most synthetics showed semi-hard to hard grain texture. Results
showed significant genetic variation among the synthetic hexaploids for protein content, SDS-S values, and grain weight and
plumpness. Quality measurement values of synthetic hexaploids were regressed on corresponding values of the emmer wheat parents.
With this offspring-parent regression, protein content and SDS-S values explained 8.7 and 28.8%, respectively, of the variation
among synthetics, indicating a significant contribution from the emmer wheat parents for these traits. The synthetic hexaploids,
in general, had significantly higher protein content (15.5%, on average) and longer grains than ‘Seri M82’, the bread wheat
control (13.1% protein content). Synthetics with SDS-S values and grain weights higher than those of ‘Seri M82’ were also
identified. Protein content among synthetics showed significantly negative correlations with grain weight and plumpness, but
no correlation with SDS-S values. Despite these negative correlations, 10 superior synthetic hexaploid wheats, derived from
nine different emmer wheat parents and with above average levels of protein content, SDS-S values, and either grain weight
or plumpness, were identified. This study shows that genetic variation for quality in tetraploid emmer wheat can be transferred
to synthetic hexaploid wheats and combined with plump grains and high grain weight, to be used for bread wheat breeding. 相似文献
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~(32)P、~(14)C在杂交水稻分蘖和穗中的分布与谷粒产量的关系 总被引:1,自引:2,他引:1
研究结果表明,高产杂交水稻有较多的~(32)P和~(14)C积集在分蘖和孔熟期的稻穗中,杂交水稻F_1乳熟期同化的~(14)C-葡萄糖向稻穗输送的百分率高。观察发现,杂交稻谷粒产量与~(14)C-葡萄糖在稻穗中分布及与IAP(输入积)之间的相关性高。低产杂交稻没有上述特性。放射性同位素~(32)P、~(14)C在分蘖和稻穗中的分布可作为预测杂交水稻F_1谷粒产量的指标。 相似文献
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辐射与杂交结合提高小麦高分子量麦谷蛋白优质亚基转化效果的研究 总被引:1,自引:0,他引:1
本文利用核质互作与辐射处理研究了促进优质HMW GS基因的转化。结果表明 ,不同组合处理间F1籽粒优质亚基 (Glu D15+1 0 )的转化存在差异 ,辐射处理的多以杂合型出现。F2 分离群体中 ,反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本 ,细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率 ,不论是哪种组合 ,反交时 5+1 0亚基的表达频率高于正交 ;无论正交还是反交 ,辐射处理提高了 5+1 0亚基的表达频率 ,而且在不同组合间存在差异。 相似文献