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1.
Regulation of phosphodiesterase synthesis: requirement for cyclic adenosine monophosphate-dependent protein kinase 总被引：7，自引：0，他引：7
Endogenous cyclic adenosine monophosphate (AMP) and its dibutyryl derivative increase cyclic AMP phosphodiesterase activity in cultured lymphoma cells. This effect is prevented by cycloheximide. A variant population of cells deficient in cyclic AMP-dependent protein kinase contains lower basal phosphodiesterase activity, which cannot be induced by cyclic AMP. 相似文献
2.
Cyclic guanosine and adenosine 3',5'-monophosphates in canine thyroid: localization by immunofluorescence 总被引：3，自引：0，他引：3
E F Fallon R Agrawal E Furth A L Steiner R Cowden 《Science (New York, N.Y.)》1974,184(4141):1089-1091
When guanosine 3',5'-monophosphate (cyclic GMP) and adenosine 3',5'-monophosphate (cyclic AMP) are localized in canine thyroid by a flurescence Immunocytochemical procedure, distinct staining patterns for each nucleotide are seen: Cyclic AMP is distributed throughout the follicular cell cytoplasm before and after administration of thyroid-stimulating hormone, while cyclic GMP is localized to the follicular cell mumbrane in the control state, and increased cytoplasmic fluorescence is visualized after acetylcholine. These data provide histological evidence that correlates with cyclic nucleotide tissue measurements, sugesting diverse roles of the two nucleotides in thyroid function. 相似文献
3.
The generation of adenosine 3',5'-monophosphate (cyclic AMP) in response to catecholamines in the 2B subclone of RGC6 rat glioma cells previously exposed to norepinephrine and refractory to further norepinephrine addition is substantially increased by addition of inhibitors of RNA and protein synthesis. The time course of the effect of these inhibitors on cyclic AMP concentration suggests that rapid protein synthesis and turnover are involved in catecholamine refractoriness. Norepinephrine induction of cyclic nucleotide phosphodiesterase is demonstrable in RGC6 cells but not in the 2B subclone. Thus, catecholamine refractoriness cannot be attributed to induction of phosphodiesterase. This implies that induction of a protein or proteins, important in catecholamine refractoriness, affects the synthesis rather than the degradation of cyclic AMP. 相似文献
4.
Protein kinase translocation as an early event in the hormonal control of uterine contraction 总被引：7，自引：0，他引：7
beta-Adrenergic stimulation with isoproterenol inhibits contractility, increases cyclic adenosine monophosphate (AMP) concentration, decreases the concentration of unsaturated cyclic AMP receptor sites, and increases cyclic AMP-independent kinase in the uterus of ovariectomized rats. The total soluble kinase activity is reduced. The protein kinase activity lost from the cytosol was translocated to the microsomal fraction mostly in a cyclic AMP-independent form, suggesting a particulate substrate for the activated enzyme. 相似文献
5.
Gonadotropin-releasing hormone: regulation of adenosine 3',5'-monophosphate in ovarian granulosa cells 总被引：1，自引：0，他引：1
The antigonadal effects of gonadotropin-releasing hormone in ovarian granulosa cells are due to attenuation of the adenosine 3',5'-monophosphate (cyclic AMP) response to follicle-stimulating hormone. Agonists of gonadotropin-releasing hormone progressively inhibit adenylate cyclase and stimulate phosphodiesterase activities in cultured granulosa cells, indicating that blockade of gonadotropin action is attributable to the combined effects of decreased production and increased degradation of cyclic AMP. 相似文献
6.
Coexistence of guanylate cyclase and atrial natriuretic factor receptor in a 180-kD protein 总被引：9，自引：0，他引：9
Atrial natriuretic factor (ANF) is a peptide hormone that is released from atria and regulates a number of physiological processes, including steroidogenesis in adrenal cortex and testes. The parallel stimulation of membrane guanylate cyclase and corticosterone production in isolated fasciculata cells of rat adrenal cortex has supported the hypothesis of a mediatory role for cyclic guanosine monophosphate (cyclic GMP) in signal transduction. A novel particulate guanylate cyclase tightly coupled with ANF receptor was purified approximately 273,000-fold by two-step affinity chromatography. The enzyme had a molecular size of 180 kilodaltons and was acidic in nature with a pI of 4.7. Its specific activity was 1800 nanomoles of cyclic GMP formed per minute per milligram of protein. The purified enzyme bound ANF with a specific binding activity of 4.01 nanomoles per milligram of protein, a value that is close to the theoretical binding activity of 5.55 nanomoles per milligram of protein for 1 mole of the ligand binding 1 mole of the receptor protein. These results indicate that the guanylate cyclase-coupled ANF receptor exists in a 180-kilodalton protein of rat adrenocortical carcinoma and represent a step toward the elucidation of the basic mechanism of cyclic GMP-mediated transmembrane signal transduction in response to a hormone. 相似文献
7.
Hormonal control of neutrophil lysosomal enzyme release: effect of epinephrine on adenosine 3',5'-monophosphate 总被引：3，自引：0，他引：3
Human neutrophilic leukocytes release neutral protease and beta-glucuronidase during cell contact with, and phagocytosis of, zymosan particles treated with rheumatoid arthritic serum. Release of lysosomal enzymes is inhibited by epinephrine and adenosine 3',5'-monophosphate (cyclic AMP), but not by phenylephrine or adenosine 5'-monophosphate. Inhibition of enzyme release by epinephrine may be mediated by cyclic AMP because the cyclic AMP in the neutrophils is increased by epinephrine treatment at the time when enzyme release is reduced. 相似文献
8.
Cyclic adenosine monophosphate in bacteria 总被引：83，自引：0，他引：83
9.
Cyclic 3',5'-nucleotide phosphodiesterase: cytochemical localization in cerebral cortex 总被引：11，自引：0，他引：11
The distribution of cyclic 3',5'-nucleotide phosphodiesterase activity in rat cerebral cortex was determined by cytochemical methods. The detectable phosphodiesterase activity was localized in postsynaptic (dendritic) nerve endings, most of it immediately adjacent to the synaptic membrane. Most of the postsynaptic nerve endings showed phosphodiesterase activity. 相似文献
10.
JIANG Dai-xun LIU Shu-rong ZHANG Mei-hua ZHANG Tao MA Wen-jing MU Xiang CHEN Wu 《农业科学学报》2015,14(1):140-147
Luteolin is an active ingredient found early from Folium perillae and Flos lonicerae, and has a specific inhibition on phosphodiesterase 4(PDE4) activity in vitro. Researches show luteolin has pharmacological effects of anti-inflammation, anti-anaphylaxis, antitumor, antioxidant, protection of nervous system and so on, and has mainly been used for the treatment of respiratory inflammatory diseases, cancer and cardiovascular disease in clinic. PDE4, specific to hydrolyze cyclic AMP(c AMP), is considered to be a new anti-inflammatory target due to the decisive role on c AMP signal in inflammatory cells such as neutrophils. In order to explore the anti-inflammatory mechanism, we further studied the effects of luteolin on the activity and expression of PDE4, the expression of lymphocyte function-associated antigen-1(LFA-1) and macrophage-1(MAC-1) in neutrophils, and the adhesion of neutrophils and endothelial cells. The results showed that luteolin had a dose-dependent inhibition on both bare PDE4 activity and PDE4 in cultured neutrophils, and had an obviously promotive effect on gene expressions of PDE4 A, 4B and 4D in later period. Luteolin had a significant inhibitory effect on neutrophils adhesion and LFA-1 expression in early stage, and had no obvious effect on MAC-1 expression. Therefore, luteolin can inhibit LFA-1 expression of neutrophils, then inhibit the adhesion of neutrophils and endothelial cells, and the mechanism is at least related with the inhibition of PDE4 activity. 相似文献
11.
Guanosine 3',5'-monophosphate in sympathetic ganglia: increase assoicated with synaptic transmission 总被引：11，自引：0，他引：11
Brief stimulation of cholinergic preganglionic nerve fibers resulted in an increase in guanosine 3',5'-monophosphate (cyclic GMP) in the bullfrog sympathetic ganglion. When the release of synaptic transmitter was prevented by a high-magnesium, low-calcium Ringer solution, stimulation of preganglionic nerve fibers did not increase cyclic GMP in the ganglion. The increase in cyclic GMP caused by preganglionic stimulation was also blocked by the muscarinic antagonist, atropine. The data indicate that the increase in cyclic GMP is associated with synaptic transmission and support the possibility that cyclic GMP may mediate the postsynaptic action of acetylcholine at muscarinic cholinergic synapses. 相似文献
12.
Cyclic nucleotide phosphodiesterase in Dictyostelium discoideum: interconversion of two enzyme forms 总被引：9，自引：0，他引：9
B M Chassy 《Science (New York, N.Y.)》1972,175(25):1016-1018
An extracellular cyclic nucleotide phosphodiesterase was isolated from either growing cultures or aggregating amoebas of Dictyostelium discoideum. The enzyme is released in a form with a low Michaelis constant (15 micromolar) and spontaneously undergoes a slow conversion to a less active form with a high Michaelis constant (2 mnillimolar). Inactivation was prevented or reversed by use of Cleland's reagent, dithiothreitol. The two enzyme forms may be part of a mechanism for control of concentration of cyclic adenosine monophosphate. 相似文献
13.
Drugs that reduce anxiety may be mediated by cyclic adenosine monophosphate in the brain because (i) potent anxiety-reducing drugs are also potent inhibitors of brain phosphodiesterase activity; (ii) dibutyryl cyclic adenosine monophosphate has the ability to reduce anxiety; (iii) the methylxanthines show significant anxiety-reducing effects; (iv) theophylline and chlordiazepoxide produce additive anxiety-reducing activity; and (v) there is a significant correlation between the anxiety-reducing property of drugs and their ability to inhibit phosphodiesterase activity in the brain. 相似文献
14.
《Science (New York, N.Y.)》1994,266(5193):1933
Figure 1, parts A and C (p. 1349), of the Research Article "Calcium-calmodulin modula- tion of the olfactory cyclic nucleotide-gated cation channel" by Mingyao Liu et al. (25 Nov., p. 1348) were incorrectly printed. The correct figures appear below. On page 1351 of the same article, in line 25 of the first column of text, "GMP" should have been "AMP." In the second line of the text on page 1352, the first "(C.Ln)o" should have been "(C.Ln)c." 相似文献
15.
The acellular slime mold Physarum polycephalum releases a soluble adenosine 3', 5'-monophosphate phosphodiesterase into the growth medium. Although this enzyme resembles the particulate diesterase of the same organism in kinetic properties and in inhibition by methyl purines, its greater stability, its insensitivity to stimulation by imidazole and to inhibition by adenosine triphosphate,- and its selective release into the medium indicate a specific function, perhaps protection against exogenous cyclic nucleotide, for the soluble enzyme. 相似文献
16.
Catecholamines and dibutyryl adenosine 3', 5'-monophosphate (dibutyryl cyclic AMP) increase the activity of myosin adenosine triphosphatase in cultured rat heart cells. Dichloroisoproterenol, an inhibitor of the beta receptor of the catecholamines, inhibits the action of the catecholamines but not of cyclic AMP. 相似文献
17.
Responses of norepiniephrine sensitive adeniosinie 3',5'-monophosphate (cyclic AMP)-generating systems in combined midbrain-striatal slices of four rat strains correlate positively with spontaneous behavioral activity and negatively with levels of midbrain and striatal tyrosine hydroxylase. Responses of cerebral cortical norepinephrine-sensitive cyclic AMP systems correlate negatively with spontaneous behavioral activity antd positively with midbrain and striatal tyrosine hydroxylase. Such correlations were not found with responses of the cyclic AMP- generatinlg systems to isoproterenol, adenosine. veratridine or of an adenosne and norepinephrine combination. 相似文献
18.
Cyclic adenosine monophosphate and hypertension in rats 总被引：7，自引：0，他引：7
M S Amer 《Science (New York, N.Y.)》1973,179(75):807-809
Aortas from spontaneously hypertensive and stress hypertensive rats contained significantly lower intracellular concentrations of cyclic adenosine monophosphate than did their respective controls. Adenylate cyclase activity was normal but was less responsive to stimulation, while phosphodiesterase activity (especially the low Michaelis-Menten constant form) was significantly elevated. Human aortas contained two forms of phosphodiesterase that were similar to those in rat aortas. 相似文献
19.
Growth arrest and morphological change of human breast cancer cells by dibutyryl cyclic AMP and L-arginine 总被引：3，自引：0，他引：3
The growth in vitro of human breast cancer cells, line MCF-7, was inhibited by a daily supplement of L-arginine (1 milligram per milliliter). Arginine acted synergistically with dibutyryl adenosine 3',5'-monophosphate (cyclic AMP) (10(-6) molar) to enhance the growth inhibitory effect: the cell replication ceased completely within 2 days after treatment. The growth arrest accompanied a change in cell morphology and was preceded by increases in the cellular concentration of cyclic AMP, adenylate cyclase, and type II cyclic AMP-dependent protein kinase activities as well as a decrease of estrogen binding activity. The results suggest that growth of human breast cancer cells is subject to cyclic AMP-mediated regulation and that arginine may play a specific role in this process. 相似文献
20.
Histone phosphorylation: stimulation by adenosine 3',5'-monophosphate 总被引：27，自引：0，他引：27
T A Langan 《Science (New York, N.Y.)》1968,162(853):579-580
Adenosine cyclic 3',5'-monophosphate at a concentration of 10-(7)M causes a four-to sixfold increase in the rate of histone phosphorylation catalyzed by a liver enzyme preparation. This observation suggests a mechanism for the induction of RNA synthesis by those hormones that cause increases in the concentration of cyclic AMP. 相似文献