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1.
应用抗猪生殖－ 呼吸道综合征病毒（ Ｐ Ｒ Ｒ Ｓ Ｖ）单克隆抗体所作的间接免疫荧光抗体试验（ Ｉ Ａ Ｆ），对人工接种 Ｐ Ｒ Ｒ Ｓ Ｖ的３ 头妊娠母猪所产９ 头死胎和２ 头新生仔猪体内的 Ｐ Ｒ Ｒ Ｓ Ｖ 抗原分布进行了观察。结果表明， Ｐ Ｒ Ｒ Ｓ Ｖ 抗原在死胎主要分布于脾脏（９／９）和淋巴结（３／４）的巨噬细胞内，而少见于肺（２／９）和肾（０／９）等其他脏 器。但新生仔猪则仅在肺脏（２／２）的巨噬细胞内检出了 Ｐ Ｒ Ｒ Ｓ Ｖ 抗原，此结果说明 Ｐ Ｒ Ｒ Ｓ Ｖ 可通过胎盘屏障垂直传播给胎儿，而且 Ｐ Ｒ Ｒ Ｓ Ｖ 对组织的嗜性在胎儿和新生仔猪之间存在差异。 相似文献
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用灌洗肺脏收集的肺泡巨噬细胞建立了检测活猪生殖－－呼吸道综合征病毒（ＰＲＲＳＶ）感染的高效敏感方法。５头１０周龄（１头）和１４周龄（４）的猪，口鼻接种口接触感洒ＰＲＲＳＶ。感染ＰＲＲＳＶ前收集每头仔猪的诊断样品在以后９周内，每周采集一次血清。第２和第４－９周收集肺居噬细胞，血清和肺泡巨噬细胞均适于早期感染的检测，但时间稍长后肺泡巨噬细胞为更好。４时仅从１头仔猪血清中分离到病毒，而从４头仔狸的肺泡巨 相似文献
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猪繁殖与呼吸系统综合征对仔猪成活率影响的探讨 总被引：1，自引：0，他引：1
猪繁殖与呼吸系统综合征（ＰＲＲＳ）是１９８７年首发于北美和欧洲的一种猪的新传染病，其病原为猪繁殖与呼吸系统病毒（ＰＲＲＳＶ）。该病主要症状为早产、死胎、木乃伊胎儿和仔猪、生长猪呼吸系统疾病。近年来，国内很多资料报道该病对新生仔猪和断奶仔猪的成活率影响极大，死亡率高达８０％。但根据某猪场暴发ＰＲＲＳ的实际情况看，仔猪成活率与资料报道的有较大差异，现报告如下。１ 发病情况某猪场是饲养大约克、长白外种猪的繁殖场，常年饲养能繁母猪９０头，后备母猪２６头，种公猪１０头。于１９９７年６月两次从省外种猪场引进… 相似文献
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猪生殖-呼吸道综合征病毒(PRRSV)分离鉴定及其与欧、美PRRSV抗原的比较 总被引：7，自引：0，他引：7
在我国吉林省某地猪生殖－呼吸道综合征病毒（ＰＲＲＳＶ）抗体阳性猪群的４头２日龄弱仔猪实质脏器中分离到２株ＰＲＲＳＶ。间接荧光抗体试验（ＩＦＡ）结果表明,ＰＲＲＳＶ（ＬＶ．ＶＲ－２３３２）抗血清与２个分离株呈阳性反应;分离到病毒的弱仔猪血清与参考株（ＬＶ．ＶＲ－２３３２）也呈阳性反应;而分离株与ＨＣＶ、ＰｒＶ、ＰＰＶ、ＴＧＥＶ、ＰＥＤＶ和ＨＥＶ无交叉抗原。以上试验证明,我们已成功地分离到２株地方性ＰＲＲＳＶ。进一步用六种ＰＲＲＳＶ单抗（Ａ～Ｆ）进行ＩＦＡ试验,结果２个分离株与ＶＲ－２３３２的荧光反应谱相同。说明它们之间在抗原结构上具有同源性。 相似文献
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猪生殖—呼吸道综合征病毒（PRRSV）分离鉴定及其与欧,美PRRSV … 总被引：2，自引：0，他引：2
在我国吉林省某地猪生殖呼吸道综合征病毒（ＰＲＲＳＶ）抗体阳性猪群的４头２日龄弱仔猪实质脏器中分离到２株ＰＲＲＳＶ,间接荧光抗体试验（ＩＦＡ）结果表明,ＰＲＲＳＶ（ＬＶ．ＶＲ－２３３２）抗血清与２个分离株呈阳性反应;分离到病毒的弱仔猪血清与参考株（ＬＶ．ＶＲ－２３３２）也呈阳性反应,而分离株与ＨＣＶ,ＰｒＶ,ＰＰＶ,ＴＧＥＶ,ＰＥＤＶ和ＨＥＶ无交叉抗原,以上试验证明,我们已成功地分离到２株地方性ＰＲ 相似文献
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本研究在国内首次成功建立了辣根过氧化物酶标记的链霉亲和素－生物系（ＬＳＡＢ）免疫组化染色法检测猪生殖－呼吸道综合征病毒（ＰＲＲＳＶ）抗原。应用ＬＳＡＢ染色技术检测１２头人工感染ＰＲＲＳＶ美洲株（ＡＴＣＣ ＶＲ－２３３２）或国内分离株（Ｂ９６－４，Ｂ９６－５）的ＳＰＦ仔猪组织细胞内的ＰＲＲＳＶ抗原，阳性检出率为１００％。 相似文献
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通过口／鼻和静脉注射途径，用猪圆环病毒（ＰＣＶ）实验性感染未吮初乳仔猪，检查其病毒和抗原的分布，分别用病毒分离和冰冻切片间接免疫荧光抗体技术检查ＰＣＶ和抗原。结果，在机体组织中都检出了ＰＣＶ抗原，特别是在脾、胸腺和肺。中枢神经系统的组织中未检出ＰＣＶ抗原或病毒。检查自然发生流产／死胎母猪的胎儿，结果从同一农场两窝死产仔猪的２份血清和１份脾脏病料中分离出３株ＰＣＶ；检查１６０份胎儿血清，未检出ＰＣＶ 相似文献
10.
从疑似PRRS流产胎儿分离PRRSV的研究 总被引：272，自引：4，他引：272
采用猪肺巨噬细胞和Ｍａｒｃ－１４５细胞培养对国内暴发流行ＰＲＲＳ的某地猪场进行了病毒分离，从流产胎儿分离出特征性致细胞病变因子，用ＰＲＲＳＶＳＤＯＷ－１７单克隆抗体进行间接免疫荧光染色，证明从４头流产胎儿分离到４株ＰＲＲＳＶ（ＣＨ－ｌａ，ＣＨ－１ｂ，ＣＨ－ｌｃ，ＣＨ－ｌｄ）这４株毒均可在猪肺巨噬细胞和Ｍａｒｃ－１４５ 细胞上生长，并产生特征性ＣＰＥ变化，用ＰＲＲＳＶ美国分离毒株ＶＲ－２３３２和ＮＶ 相似文献
11.
Experimental inoculation of late term pregnant sows with a field isolate of porcine reproductive and respiratory syndrome vaccine-derived virus. 总被引：16，自引：0，他引：16
J Nielsen A B?tner V Bille-Hansen M B Oleksiewicz T Storgaard 《Veterinary microbiology》2002,84(1-2):1-13
The use of a live attenuated porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in piglets has been associated with reproductive disorders in non-vaccinated sows. Vaccine-derived virus (VDV) has been isolated from foetuses, stillborn pigs, and dead piglets, indicating that the live vaccine spread from vaccinated piglets to non-vaccinated sows, and that the virus might be implicated in the severe reproductive problems observed. In the present study, one such VDV isolate was used to experimentally infect pregnant sows in the last trimester. The chosen isolate, which had more than 99.6% identity to the attenuated vaccine virus, originated from the lungs of a stillborn pig from a swine herd with a sudden high level of stillborn pigs and increased piglet mortality in the nursing period. Intranasal inoculation of sows with the virus isolate resulted in congenital infection, foetal death, and preweaning pig mortality. As such, the present study showed that vaccine-derived PRRSV can cause disease in swine consistent with PRRS. 相似文献
12.
Immunohistochemical detection of porcine reproductive and respiratory syndrome virus using colloidal gold. 总被引：11，自引：2，他引：9 
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下载免费PDF全文 Two cytopathic agents were isolated on porcine alveolar macrophages following inoculation with homogenates of lung tissues from pigs showing respiratory problems. These isolates were identified as porcine reproductive and respiratory syndrome (PRRS) virus isolates by indirect immunofluorescence using a PRRS virus (PRRSV) specific monoclonal antibody (MAb) and were designated as LHVA-92-1 and LHVA-92-2. Immunogold electron microscopy using a porcine PRRS positive serum pool and protein A-gold resulted in an intense labelling of aggregates of viral particles. Dark specific cytoplasmic staining of porcine alveolar macrophages infected with both virus isolates could be observed by immunogold silver staining (IGSS) using the specific MAb. This method proved effective in detecting PRRSV antigens in several ethanol-fixed tissues of piglets intranasally inoculated with the supernatants of macrophages infected with each isolate. Immunogold silver staining was also successfully used for the detection of PRRSV antigens on sections of formalin-fixed paraffin-embedded lung tissues and on frozen sections of lungs. The present results indicate that colloidal gold may be useful for the identification and immunohistochemical detection of PRRSV in tissues. 相似文献
13.
Maldonado J Segalés J Martínez-Puig D Calsamiglia M Riera P Domingo M Artigas C 《Veterinary journal (London, England : 1997)》2005,169(3):23-456
The objective of the present study was to determine the presence of recognised abortifacient viruses such as porcine reproductive and respiratory virus (PRRSV), Aujeszky's disease virus (ADV), porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2), in tissues from aborted fetuses and stillborn neonates in cases of late reproductive failure in swine. A total of 293 specimens (fetuses aborted in the last third of gestation and stillborn piglets) from 100 different cases of late-term abortions and premature farrowing from 15 different Spanish provinces were studied. PRRSV was detected in 9/100 cases by RT-PCR. Only 1/100 cases analysed (corresponding to a late-term aborted fetus with a negative PRRSV RT-PCR result) was positive for PCV2 by PCR. Neither ADV (monitored by viral isolation plus antigen detection) nor PPV (monitored by ELISA antigen capture test) infection was identified. The results suggest that PRRSV is one of the most important infectious agents, if not the most relevant one, associated with fetal infection leading to abortion or premature farrowing in Spain. Moreover, other viral pathogens such as ADV, PPV and PCV2 seem to have a minor impact on reproductive disease. 相似文献
14.
In Denmark, a porcine reproductive and respiratory syndrome virus (PRRSV) control programme, comprising vaccination of seropositive herds with a live American type PRRSV vaccine, was started in 1996. In several of these herds, spread of vaccine virus from vaccinated 3-18 week old pigs to non-vaccinated sows was demonstrated by the isolation of vaccine virus from fetuses and stillborn piglets. Surprisingly, sows infected with the American type vaccine strain consistently exhibited significantly stronger serological responses towards European type PRRSV than American type PRRSV. In order to elucidate whether the unexpectedly strong serological reaction towards European-type PRRSV in American type PRRSV infected sows was due to a booster reaction, or reactivation of an unrecognized, latent infection in the sows with European type PRRSV, a challenge study with the vaccine was carried out. In this study, the stronger serological response towards European type PRRSV than towards American type PRRSV was reproduced, and reactivation of the previous natural infection with European PRRSV could neither be demonstrated by virus isolation nor by RT-PCR. So, the increase in antibody titers towards European PRRSV in previously European PRRSV infected pigs after challenge with the vaccine strain seems to be the result of a boosting effect on the immune system, induced by the heterologous vaccine PRRSV strain. 相似文献
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W T Christianson J E Collins D A Benfield L Harris D E Gorcyca D W Chladek R B Morrison H S Joo 《American journal of veterinary research》1992,53(4):485-488
The purpose of this study was to experimentally reproduce swine infertility and respiratory syndrome (SIRS). Six multiparous sows were intranasally inoculated at 93 days of gestation with lung homogenates from clinically affected pigs, and 3 additional sows were similarly inoculated with a virus isolated in cell culture from the lung homogenate (SIRS virus, isolate ATCC VR-2332). Inoculated sows developed transient anorexia, farrowed up to 7 days prematurely, and delivered a mean of 5.8 live pigs and 6.0 dead fetuses/litter. Clinical signs of disease were not observed in 3 sham-inoculated control sows that delivered a mean of 12.7 live pigs and 0.3 stillborn fetuses/litter. The SIRS virus was isolated from 50 of 76 live-born and stillborn fetuses from the 9 infected litters. Virus was not isolated from 26 autolyzed fetuses or 15 control pigs. Six of 9 inoculated sows developed neutralizing antibodies to SIRS virus. The reproductive effects found in these experiments were identical to those found in field cases. On the basis of our findings, virus isolate ATCC VR-2332 causes the reproductive failure associated with SIRS. 相似文献
16.
C A Bolin J A Cassells 《Journal of the American Veterinary Medical Association》1990,196(10):1601-1604
Leptospira interrogans serovar bratislava was isolated from a herd of swine in Iowa with a history of stillborn and weak neonatal pigs. Placentas, kidneys, and lungs of stillborn and weak pigs from 3 litters were processed to detect leptospires by use of bacteriologic culture and fluorescent antibody testing. Sera from stillborn and weak pigs were tested to detect agglutinating antibody against leptospires. A low antibody titer against L interrogans serovar bratislava was detected in the sera of stillborn and weak pigs. Small numbers of leptospires were sometimes detected in tissues by use of the fluorescent antibody test. Serovar bratislava was isolated from placentas, stillborn pigs or weak pigs from each of the 3 litters. 相似文献
17.
A retrospective study on pig lung tissues from 60 cases of proliferative and necrotizing pneumonia (PNP) was performed to determine the presence of porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), and porcine circovirus type 2 (PCV2) in these lesions. Cases selected included 30 cases diagnosed between 1988 and 1992 and 30 cases diagnosed between 1997 and 2001. In each group of 30 cases, 10 were from suckling piglets, whereas the other 20 were from postweaned animals representing either nursery or grower-finisher pigs. Immunohistochemistry using a monoclonal antibody to influenza virus type A was used to determine the presence of SIV, and in situ hybridization was used for the detection of PRRSV and PCV2 nucleic acids. PRRSV was detected in 55 of the 60 cases examined (92%), PCV2 in 25 cases (42%), and SIV in only 1 case (2%). In 30 cases (50%), PRRSV was the only virus detected, whereas in 25 other cases (42%), a combination of PRRSV and PCV2 could be detected in the lungs with PNP lesions. PCV2 could not be detected in the lungs of suckling pigs with PNP. All PCV2-positive cases were found in postweaned pigs and were always in combination with PRRSV. In this latter age group, PCV2 was detected in 63% of the cases (25/40). Data from our study indicate that SIV is rarely identified in PNP and that PCV2 infection is not essential for the development of PNP lesions. The results of the present study demonstrate that PRRSV is consistently and predominantly associated with PNP and should be considered the key etiologic agent for the condition. 相似文献
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Inger M Brunborg Christine M Jonassen Torfinn Moldal Bj?rn Bratberg Bj?rn Lium Frank Koenen Jürgen Sch?nheit 《Journal of veterinary diagnostic investigation》2007,19(4):368-375
During a period of 1.5 months, a newly established pig herd experienced a high number of mummifications and stillbirths, a high neonatal mortality rate, and many piglets with congenital tremors or hind leg ataxia. After clinical and histological investigations, the submitted animals were divided into 4 groups: mummified or stillborn (N = 6), live born with myocarditis (N = 5) (average age 22.8 days), live born without myocarditis (N = 14) (average age 20.0 days), and control animals from a different herd (N = 5) (newborn). Statistically significant differences were observed in the mean porcine circovirus 2 (PCV2) load among the 4 groups in the liver (P < 0.0001). The presence of PCV2 antigen within the myocardial lesions was confirmed by immunohistochemistry. A high load of PCV2 DNA was observed in myocardium, liver, and spleen from mummified or stillborn piglets (>1 x 10(7) copies per 500 ng DNA), lower in piglets with myocarditis (>1 x 10(5) copies per 500 ng DNA), and even further lower in pigs without myocarditis (<1 x 10(5) copies per 500 ng DNA), whereas no PCV2 DNA was detected in the control animals. Myocardium, liver, and spleen were well suited for routine testing of fetuses and young piglets by quantitative real-time polymerase chain reaction. Neither porcine parvovirus nor encepaholomyocarditis virus was detected. These results indicate that the PCV2 infection might have been of etiological importance for the fetal deaths and piglet mortality observed in this herd. 相似文献
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Chang HW Jeng CR Liu JJ Lin TL Chang CC Chia MY Tsai YC Pang VF 《Veterinary microbiology》2005,108(3-4):167-177
Two common viral pathogens of swine, namely, porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), were investigated in regard to their effects on monolayer cultures of swine alveolar macrophages (AMs). The purpose was to identify selected cellular changes and responses potentially associated with the clinical reactions of pigs infected with either or both of these viruses. Measurements included the (1) absolute and relative numbers of infected, viable, and apoptotic cells; (2) distribution of viral antigens; (3) levels of interferon-alpha (IFN-alpha) and tumor necrosis factor-alpha (TNF-alpha) produced and their association with the extent of virus-induced cytopathology. Four groups of AMs were studied, including mock-infected, PCV2 alone-infected (PCV2-A), PRRSV alone-infected (PRRSV-A), and PCV2 and PRRSV dually infected (PCV2/PRRSV) groups. The AMs of PCV2-A group had high antigen-containing rate without cell death. There was a marked increase in cell death and apoptosis in PRRSV-A group. However, a lower PRRSV-induced infectious rate, cell death, and apoptosis were seen in PCV2/PRRSV group. High levels of IFN-alpha production were detected in PCV2-infected groups, but not in mock-infected and PRRSV-A groups. The PRRSV-induced cytopathic effect (CPE) on MARC-145 cells or swine AMs was markedly reduced by pre-incubation of the cells with UV-treated or non-UV-treated supernatants of PCV2-infected AMs. In addition, the reduction in CPE was abolished when the supernatants of PCV2-infected AMs were pre-treated with a mouse anti-recombinant porcine IFN-alpha antibody. The results suggest that swine AMs were an important reservoir of PCV2; PCV2 infection reduced PRRSV infection and PRRSV-associated CPE in PCV2/PRRSV AMs; the reduction of PRRSV infection in AMs was mediated by IFN-alpha generated by PCV2 infection. The reduced PRRSV-associated CPE in AMs and increased pro-inflammatory cytokine production may lead to a more severe pneumonic lesion in those dually infected pigs. 相似文献