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花生野生近缘种生物素羧基载体蛋白基因的克隆与结构分析 总被引：1，自引：0，他引：1
根据栽培花生鲁花14生物素羧基载体蛋白基因accB1和accB2 cDNA序列设计基因特异引物,克隆了花生野生近缘种Arachis duranensis、A.rigonii、A.batizocoi和A.hoehnei的accB1和accB2 cDNA 序列.序列分析表明,accB1和accB2在栽培花生和野生近缘种中高度保守,氨基酸序列一致性分别高于98.6%和97.5%. A基因组A.duranensis、A.rigonii和B 基因组A.batizocoi、A.hoehnei 的accB1和accB2的氨基酸序列存在差异,但与基因组来源无关.花生野生近缘种A.duranensis、A.rigonii、A.batizocoi和A.hoehnei accB2基因组序列分析表明,栽培花生和野生近缘种的accB2的基因结构相同. A基因组的A.duranensis与B 基因组的A.batizocoi和A.hoehnei的accB2 基因组DNA核苷酸序列一致性分别为98.9%,98.8%,而A 基因组的A.rigonii与A.duranensis、A.batizocoi和A.hoehnei 的序列一致性分别为93.5%,93.6%,93.4%.研究表明,生物素羧基载体蛋白基因在栽培花生及野生近缘种中非常保守. 相似文献
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东北地区大豆品种资源脂肪酸组成的分析研究 总被引：11，自引：1，他引：11
测定2341份我国东北地区大豆品种资源5种脂肪酸含量。以亚油酸含量最高,45—62%。平均含量依次为亚油酸>油酸>棕榈酸>亚麻酸>硬脂酸。分析比较结果,各脂肪酸含量,不同种皮色、脐色、结荚习性、叶形和花色品种间存在显著差异。棕榈酸、油酸、亚油酸和亚麻酸含量,不同栽培类型、不同粒形品种间有显著差异。硬脂酸、油酸和亚麻酸 相似文献
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花生种子发育过程中脂肪酸积累规律的研究 总被引：3，自引：0，他引：3
以鲁花14为材料,对花生种子发育过程中荚果和种子的发育情况以及花生种子脂肪酸的累积过程进行了研究。结果表明,在果针入土的第39天,小种子(远离果针一端)的平均长度超过大种子(靠近果针一端),在接近成熟时,小种子的平均质量超过大种子。在花生种子中,可以检测到12种脂肪酸。按含量由高到低依次为油酸、亚油酸、棕榈酸、硬脂酸、山萮酸、花生酸、二十四烷酸、花生烯酸、异油酸、亚麻酸、棕榈油酸、豆蔻酸。在花生种子发育过程中,脂肪酸含量随种子的发育逐渐增加,油酸含量不断增多,而亚油酸则呈缓慢减少的趋势。在接近成熟时,油酸和亚油酸占全部脂肪酸含量的79.4%。油酸亚油酸的比值随着种子的发育呈逐渐增大的变化规律。 相似文献
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杨学文 《粮油仓储科技通讯》2012,(3):35-37
油茶籽油作为具有食疗效果的油脂,市场发展前景非常广阔。本文对江西地区2011年产的38个油茶籽的含油率和脂肪酸组成进行了分析。结果表明:油茶籽含油率在20.48%～49.84%之间,平均为37.07%。油茶籽油脂肪酸主要是由棕榈酸、硬脂酸、油酸、亚油酸、花生一烯酸等脂肪酸组成,棕榈酸含量平均值8.19%;硬脂酸含量平均值2.48%;油酸含量平均值81.02%;亚油酸含量平均值7.74%;花生一烯酸含量平均值0.46%。主要不饱和脂肪酸油酸和亚油酸含量平均达到了88.76%,说明江西地区的油茶籽油符合高品质食用油的标准。 相似文献
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花生是主要的油料作物,脂肪酸组成受环境等的影响而不稳定。本研究选取60份黄淮及长江流域产区主推的花生品种,连续2年在湖北武汉、河北石家庄、河南濮阳和河南周口4个环境下种植,利用GB/T5510-2011法检测种子脂肪酸含量。结果表明,高油酸花生品种油酸含量比普通油酸品种稳定,普通油酸品种棕榈酸和亚油酸含量比高油酸品种稳定;武汉种植环境有利于花生油酸含量的提高,2年60份品种的平均油酸含量均最高,分别为52.93%和52.64%;高油酸花生品种除油酸含量显著提高外,花生烯酸含量也提高了54.1%;而棕榈酸和亚油酸含量分别降低了45.20%和90.44%。结合前期SSR研究结果,本研究涉及的6份高油酸品种属于不同的类群(G1、G2c和G2e),其遗传背景差异较大。本研究结果为花生品种的合理布局和进一步的遗传改良提供了理论依据。 相似文献
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野生花生抗青枯病种质的发掘及分子鉴定 总被引：11，自引：0，他引：11
以花生属5个区组的79份野生花生种质为材料,系统鉴定了野生花生对青枯病的抗性反应,从中发掘高抗青枯病的种质15份,含匍匐区组种质3份、直立区组1份、异形花区组1份、花生区组8份、未命名种质2份,抗病材料频率达到19%,高于栽培种花生资源的抗性频率。通过SSR分析表明,在所获得的抗青枯病野生花生材料中,四倍体野生种A.monticola与栽培种花生的亲缘关系最近,其次为花生区组的二倍体野生种A.duranensis和A.chacoense。根据DNA扩增结果,绘制了抗青枯病种质的指纹图谱,明确了其SSR分子特性。 相似文献
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对山东省379份花生种质资源的品质分析表明,脂肪和蛋白质含量在类型间存在着一定差异,筛选出高脂肪(53.0%以上)材料6份,高蛋白质(33.0%以上)材料8份。脂肪酸和氨基酸的分析表明,普通型花生品种品质优于其他类型,其中油酸/亚油酸比值均在1.6以上,个别的达2.0以上,17种氨基酸中有12种以普通型品种含量最高,对今后花生品种的品质改良提出了初步设思。 相似文献
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对四川省198份花生种质资源品质特性的研究表明,我省花生种质资源中有丰富的高(低)蛋白质、脂肪、油酸、亚油酸材料,尤以龙生型品种、地方品种品质最好,具有蛋白质含量高、油酸/亚油酸比值大的特点,是品质育种的好亲本,而且在深度加工开发等方面有着广阔的前景。 相似文献
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大豆脂肪酸组分的胚、细胞质和母体遗传效应分析 总被引：1，自引：0，他引：1
利用5个大豆品种配制20个杂交组合,采用广义种子遗传模型分析了大豆脂肪酸组分的胚、细胞质和母体植株等3套遗传体系的基因主效应和基因型×环境效应。棕榈酸含量、硬脂酸含量和亚油酸含量是以基因型×环境互作效应为主。亚麻酸和油酸的遗传主效应和基因型×环境互作效应相近。在脂肪酸组分的遗传主效应中,棕榈酸、硬脂酸和亚油酸含量是以胚主效应为主。油酸含量和亚麻酸含量以细胞质主效应为主。在基因型×环境互作方差中,脂肪酸组分以极显著的胚互作方差为主。亚麻酸含量是以基因的加性效应和加性×环境互作效应为主,棕榈酸含量、硬脂酸含量、油酸含量和亚油酸含量以基因的显性和显性×环境互作效应为主。棕榈酸含量和油酸含量是以普通狭义遗传率为主。硬脂酸、亚油酸含量和亚麻酸含量以互作狭义遗传率为主。在普通狭义遗传率中,棕榈酸含量、油酸含量和亚麻酸含量以细胞质普通遗传率和母体普通遗传率为主。在互作狭义遗传率中,油酸含量和亚麻酸含量以胚互作狭义遗传率为主,亚油酸含量以母体植株互作遗传率为主。棕榈酸含量、硬脂酸含量、油酸含量和亚油酸含量以细胞质及母体选择响应和互作选择响应为主,亚麻酸含量的胚普通选择响应和互作选择响应为主。 相似文献
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Sandip K. Bera Jignesh H. Kamdar Swati V. Kasundra Pitabas Dash Anil K. Maurya Mital D. Jasani Ajay B. Chandrashekar N. Manivannan R. P. Vasanthi K. L. Dobariya Manish K. Pandey Pasupuleti Janila T. Radhakrishnan Rajeev K. Varshney 《Euphytica》2018,214(9):162
Peanut plays a key role to the livelihood of millions in the world especially in Arid and Semi-Arid regions. Peanut with high oleic acid content aids to increase shelf-life of peanut oil as well as food products and extends major health benefits to the consumers. In peanut, ahFAD2 gene controls quantity of two major fatty acids viz, oleic and linoleic acids. These two fatty acids together with palmitic acid constitute 90% fat composition in peanut and regulate the quality of peanut oil. Here, two ahfad2 alleles from SunOleic 95R were introgressed into ICGV 05141 using marker-assisted selection. Marker-assisted breeding effectively increased oleic acid and oleic to linoleic acid ratio in recombinant lines up to 44% and 30%, respectively as compared to ICGV 05141. In addition to improved oil quality, the recombinant lines also had superiority in pod yield together with desired pod/seed attributes. Realizing the health benefits and ever increasing demand in domestic and international market, the high oleic peanut recombinant lines will certainly boost the economical benefits to the Indian farmers in addition to ensuring availability of high oleic peanuts to the traders and industry. 相似文献
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油茶生长发育过程中脂肪酸成分的测定分析 总被引：6，自引：2，他引：4
为了研究油茶生长发育过程中脂肪含量及脂肪酸成分的变化,分析测定2个油茶品种在不同生长时期油茶籽中的脂肪酸成分。采用索氏提取法提取油茶籽中的脂溶性成分,经甲酯化处理后用气象色谱分离和鉴定,运用面积归一法确定各成分的相对百分含量。结果表明,在整个发育过程中油脂形成有两个高峰,分别在9月上旬和10月上旬;种子种脂肪酸的主要组成是不饱和脂肪酸,油酸含量最高,其次是亚油酸和油酸,饱和脂肪酸以棕榈酸和硬脂酸为主,其中油酸呈明显上升趋势,棕榈酸和亚油酸呈明显下降趋势,硬脂酸呈小幅上升趋势,亚麻酸呈小幅下降趋势。 相似文献
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A study was conducted to investigate the effect of different planting dates (25 April, 5 May and 15 May 1998 and 30 April, 15 May and 25 May 1999) on the seed yield, oil content and fatty acid composition of three safflower (Carthamus tinctorius) cultivars (Yenice 5‐38, Dincer 5‐118 and 5‐154) grown in fields of the research facility of Akdeniz University in Antalya, Turkey. While seed yield, oil content, and palmitic acid, stearic acid, and oleic acid contents decreased, linoleic acid content increased from 50.86 to 55.72 % with delay in planting date. The effect of genotype on fatty acids was greater than that of environment. 相似文献
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Survey of safflower (Carthamus tinctorius L.) germplasm for variants in fatty acid composition and other seed characters 总被引：1，自引：0，他引：1
Summary Two hundred safflower accessions, originated in 37 countries, and multiplied in two environments, were evaluated for fatty acid composition of the seed oil and other seed characters. Overall mean values of stearic and palmitic acids were similar in both environments but differed for seed weight and oil content. Oleic and linoleic acids showed also similar overall mean content in both environments but some entries with intermediate contents of these acids displayed significant variation among environments. Oleic and linoleic acids showed a tremendous range of variation, from 3.1 to 90.60% and from 3.9 to 88.8%, respectively. The ranges of variation observed for stearic, oleic and linoleic acids indicate that all the reccessive genes, already discovered, controlling high content of these acids, st, ol and li, are present in the collection. Moreover, the upper values of oleic, ten points higher than the published values for the high oleic genotype olol, suggest than other genes controlling such levels may be present. 相似文献
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RFLP analysis of genetic variation in species of section Arachis, genus Arachis (Leguminosae) 总被引：1，自引：0，他引：1
Marcos A. Gimenes Catalina R. Lopes Maria L. Galgaro Jose F.M. Valls Gary Kochert 《Euphytica》2002,123(3):421-429
Four A-genome species of the genus Arachis (A. cardenasii, A. correntina, A. duranensis, A. kempff-mercadoi), three B genomes species (A. batizocoi, A. ipaënsis and A. magna),the AABB allotetraploid A. hypogaea (cultivated peanut) and introgression lines resulting from a cross between A. hypogaea and A. cardenasii were analyzed by RFLP. The A genome species (cytologically characterized by the presence of a small chromosome pair ‘A’) were closely similar to each other and shared a large number of restriction fragments. In contrast, the B genome species differed more from one another and shared few fragments. The results of this study indicate that the absence of the small chromosome pair is not a good criterion for grouping species of section Arachis as B genome species, since their genome might be quite distinct from the B genome of A. hypogaea.The lowest genetic variation was detected within accessions of A. duranensis (17 accessions), followed by A. batizocoi (4 accessions) and A. cardenasii (9 plants of accession GKP 10017).The high level of genetic variation found in A. cardenasii might indicate that not all accessions of wild species of Arachis are autogamous, as reported for A. hypogaea. 相似文献
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A greenhouse study was conducted to determine the effect of nitrogen supply (30, 100 or 170 ppm N) and raceme position on the fatty acid composition of oil extracted from erucic acid-free summer rape seed ( Brassica napus cv. Callypso ). The seven fatty acids analyzed for include palmitic, palmitolcic, stearic, oleic, linoleic, linolemc, and eicosenoic acids; of which oleic (59.54–64.84 %) and palmitoleic (0.36–0.4 %) acids were the highest and lowest levels respectively. Generally, N nutrition influenced fatty acid pattern only to a little extent. Palmitic, palmitoleic and stearic acid levels were increased by 170 ppm N, depending on raceme position, but oleic and linolenic acids were unaffected. Similarly, 170 ppm N produced the highest fatty acid levels in seeds on the lower portions of racemes, with the exception of oleic acid. This was also true in the case of the upper portions of racemes, except that 30 ppm N produced the highest levels of oleic and linoleic acids in rape seeds. Under the optimum N supply level (i.e. 100 ppm N), position of raceme on the rape plant did not greatly influence the levels of different fatty acids in lipids. 相似文献