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ObjectiveTo characterise the effects of alfaxalone by intramuscular (IM) injection in red-eared slider turtles and the influence of body temperature on anaesthetic duration and depth.Study designProspective, randomised part-blinded experimental trial.AnimalsTen healthy adult female red-eared sliders.MethodsEach turtle was anaesthetized four times with 10 and 20 mg kg?1 alfaxalone at 20 and 35 °C respectively. Time to maximal effect and plateau and recovery periods were recorded. Skeletal muscle tone, presence of various reflexes, response to noxious stimuli, and heart rate were assessed.ResultsResults are given for protocols 10 mg kg?1 20 °C; 20 mg kg?1 20 °C; 10 mg kg?1 35 °C and 20 mg kg?1 35 °C, respectively: mean time (±SD) to maximal effect was 16 ± 8, 19 ± 6, 5 ± 2 and 7 ± 5 minutes; duration of the plateau phase was 13 ± 12, 28 ± 13, 8 ± 5 and 8 ± 5 minutes and recovery time was 76 ± 20, 126 ± 17, 28 ± 9 and 41 ± 20 minutes. Endotracheal intubation was successful in 80%, 100%, 0% and 30% of turtles, respectively. At 35 °C, all animals retained nociceptive sensation in the front limbs, hind limbs and vent, whereas at 20 °C a few turtles lost peripheral nociceptive sensation. Corneal and tap reflexes were retained in all trials. Mean heart rates were 30 ± 2 and 66 ± 4 beats minute?1 at 20 and 35 °C, respectively.Conclusions and clinical relevanceAlfaxalone administered IM in red-eared sliders provided smooth, rapid induction and uneventful recovery. At 35 °C either dosage provided only short (5–10 minutes) and light sedation. At 20 °C, 10 mg kg?1 provided sedation suitable for short non-invasive procedures. About 20 mg kg?1 provided anaesthesia of approximately 20 minutes duration, appropriate for induction of inhalational anaesthesia or for brief surgical procedures with supplemental analgesia.  相似文献   

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全球海龟资源和保护概况   总被引:4,自引:0,他引:4  
世界现存海龟仅2科6属7种,都属国际濒危物种。生物界很难找到像海龟—类受到众多世人强烈关注的动物了,国际上亦十分重视海龟的保护工作,不仅专门成立了海龟专家小组(MTSG),制定了许多国际公约,定期出版《海龟通讯》(MTN)等期刊,还成立了一些影响力广泛的国际组织,并经常召开一些与海龟保护相关的国际会议,掀起了全球范围的拯救海龟大行动。本研究概述了海龟资源现状、致危因素、国际保护措施及成果。近年来全球海龟资源总量呈直线下降趋势,海龟面临的主要生存威胁有:渔业副获、直接捕食、沿海经济发展、环境污染与疾病、全球变暖等。最后,简要介绍了海龟重要的经济、科研、观赏、文化和生态价值,并呼吁全人类共同保护海龟。  相似文献   

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巴西彩龟对人造环境适应性的试验研究   总被引:3,自引:0,他引:3  
在特产动物中,巴西龟具有美食、食疗、观赏的价值已有报道。饲养者逐步增多,多以在自然环境条件下小规模繁殖饲养。笔者通过人造环境饲养试验,进行较为细致的饲养试验观察,初步尝试巴西龟高密度控温饲养的可行性,掌握巴西龟和本地乌龟的基本特征及巴西龟的一些基本生物特性,摄食生理特性和对环境变化的反应。为开展人造环境条件下饲养巴西龟寻找有效途径。  相似文献   

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The black marsh turtle (Geoemydidae: Siebenrockiella crassicollis) is a freshwater turtle that occurs in equatorial tropical climates in South East Asia. The semen of S. crassicollis was investigated by electroejaculation. The spermatozoa of S. crassicollis are filiform in shape with curved heads. The entire length, midpiece to tail length, tail width and tail length of the spermatozoa were 71.33 ± 1.55 μm, 49.92 ± 1.13 μm, 0.43 ± 0.02 μm and 48.53 ± 0.25 μm, respectively. The head length, head width across the middle and head width across the base were 14.00 ± 0.38 μm, 0.79 ± 0.03 μm and 0.91 ±0.0.03 μm, respectively. The acrosomal region of the S. crassicollis spermatozoa was narrower than the head, with an acrosomal length and width at the annulus of 2.90 ± 0.13 μm and 0.43 ± 0.01 μm, respectively. The midpiece of the S. crassicollis spermatozoa was narrower than the head and contained 30–40 mitochondrial balls, each with a ball diameter of 0.16 ± 0.002 μm. The midpiece length, midpiece width and tail length were 4.92 ± 0.16, 0.78 ± 0.03 and 48.53 ± 0.25 μm, respectively. This study presents the characteristic appearance of a freshwater turtle spermatozoa in Southeast Asia, as observed under an electron microscope. The spermatozoa of Siebenrockiella crassicollis are morphologically different from those of other freshwater turtles from other regions described in previous studies.  相似文献   

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人工饲养环境下斑鳖产卵规律的研究   总被引:1,自引:0,他引:1  
斑鳖目前仅在中国和越南的动物园中有4只存活个体,为了拯救这一物种,中国的苏州动物园和长沙动物园开展了斑鳖联合繁育项目。在2008~2011年对斑鳖的产卵时间、产卵窝数、产卵数量和卵的重量、直径等进行了测量。结果表明,雌性斑鳖在夜间产卵,有护卵行为。每年可产卵2—4窝,首次产卵在5月底或6月中旬,最晚在7月中旬结束。产卵间隔变化较大,最短的为12d,最长的达到36d,平均18.4±8.3d。每窝产卵数45~70枚,平均为60.3±7.5枚,年产卵总数在100~200枚之间,年度之间波动很大,在1~136枚之间。卯的重量在19.0-21.5g之间,平均20.1±0.7g;卵的直径在31.5~34.4mm之间,平均为32.6±0.7mm。产卵次数、窝卵数、卯重和大小都会受到运输或环境变化的影响。以上结果表明,目前提供的环境条件和饲养管理符合斑鳖的繁殖要求,但是管理中要注意减少运输应激和环境的改变。  相似文献   

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The heads of three loggerhead sea turtles were disarticulated and imaged immediately to minimize postmortem changes and then frozen and sectioned. For computed tomography (CT) imaging, the heads were positioned in ventral recumbency. Transverse CT images with soft-tissue window were obtained from the olfactory sac region to the temporomandibular joint region. After CT imaging, the heads were sectioned and the gross sections were compared to CT images, to assist in the accurate identification of the anatomic structures. Different clinically relevant anatomic structures were identified and labelled in two series of photographs (CT images and anatomic cross-sections). CT images provided good differentiation between the bones and the soft tissues of the head. The information presented in this paper should serve as an initial reference to evaluate CT images of the head of the loggerhead sea turtle and to assist in the interpretation of lesions of this region.  相似文献   

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应用光镜和电镜技术,系统观察雌性中华鳖输卵管精子储存情况,显示与精子储存有关组织结构与细胞形态。结果表明,精子储存在雌性中华鳖输卵管的蛋白分泌部后部至子宫部,但各段的组织结构及精子储存量存在一定差别。蛋白分泌部后部上皮较发达,由典型的高柱状纤毛细胞和分泌细胞构成,固有膜中腺体多为泡状腺。上皮和腺体中含有大量高密度的膜性分泌颗粒,此段只有少量的精子储存。峡部较窄且固有膜内无腺体,上皮排列紧密并呈迷路样迂回分布,上皮细胞内高电子密度分泌颗粒成团集中分布在核上方。峡部管腔中分布着大量的精子,靠近管腔的精子或精子头部嵌入上皮纤毛之间或顶端凹陷的胞质中,且精子嵌入部分的细胞结构保持完整。子宫前部形成垂直于管腔的储精小管(SST),子宫上皮及此段SST上皮的分泌颗粒电子密度不均,含高密度电子致密斑,腺体中的分泌颗粒也呈现不同的内部结构。子宫部及SST的管腔中储存有大量精子。这些区段复杂的细胞结构及分泌活动,可能在精子储存中发挥重要作用。  相似文献   

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中华鳖是我国特种经济动物,脂肪在鱼类生长中占据举足轻重的作用,充当脂溶性维生素载体的角色。文章综合国内外文献关于中华鳖对脂肪的需求,中华鳖高脂饲料对中华鳖抗氧化能力和功能基因的影响进行综述,从中华鳖的营养和免疫学两个层面上进行研究,为中华鳖饲料中脂肪的水平和组成提供参考,以促进中华鳖养殖业的发展。  相似文献   

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The purpose of this study was to evaluate the pharmacokinetics of cefquinome (CFQ ) following single intravenous (IV ) or intramuscular (IM ) injections of 2 mg/kg body weight in red‐eared slider turtles. Plasma concentrations of CFQ were determined by high‐performance liquid chromatography and analyzed using noncompartmental methods. The pharmacokinetic parameters following IV injection were as follows: elimination half‐life (t 1/2λz) 21.73 ± 4.95 hr, volume of distribution at steady‐state (V dss) 0.37 ± 0.11 L/kg, area under the plasma concentration–time curve (AUC 0–∞) 163 ± 32 μg hr?1 ml?1, and total body clearance (ClT) 12.66 ± 2.51 ml hr?1 kg?1. The pharmacokinetic parameters after IM injection were as follows: peak plasma concentration (C max) 3.94 ± 0.84 μg/ml, time to peak concentration (T max) 3 hr, t 1/2λz 26.90 ± 4.33 hr, and AUC 0–∞ 145 ± 48 μg hr?1 ml?1. The bioavailability after IM injection was 88%. Data suggest that CFQ has a favorable pharmacokinetic profile with a long half‐life and a high bioavailability in red‐eared slider turtles. Further studies are needed to establish a multiple dosage regimen and evaluate clinical efficacy.  相似文献   

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野生金头闭壳龟的资源现状调查   总被引:3,自引:1,他引:3  
张方  吴孝兵 《野生动物》2005,26(5):51-54
笔者于2001-2004年对金头闭壳龟(Cuoraaurocapitata)的野生种群的分布、数量及相关生态学进行了专项调查。结果表明,金头闭壳龟目前仅分布于青弋江源头和上游即北纬30°-30°52′的少数支流中,具体为安徽省泾县的古坝、蔡村和孤峰三地的山涧溪流以及黟县红星乡的清溪河流域。野外种群数量十分稀少,目前已不足400只。金头闭壳龟主要栖息在水质清澈、两侧植被茂密或一侧山脚多石缝的山涧溪流中。经对曾经发现过金头闭壳龟典型地带的各种生态指标的测量表明,泾县古坝乡沈村上沈村段的古坝河段的以及蔡村乡的东园至爱民段的丁溪河段,相比而言仍不失为金头闭壳龟较为理想的栖息地。  相似文献   

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平胸龟个体生殖力及资源合理利用的探索   总被引:1,自引:0,他引:1  
研究了平胸龟产卵量与个体的体长、体宽、体重等生物学指标之间的关系,并对其人工增殖、养殖及资源合理利用进行了初步探讨。结果表明,平胸龟亲本个体在自然繁殖过程中,从性成熟开始产卵量随着体重、体长和体宽的增加而增加,分别在体重(1631±27)g、体长(25.20±0.26)cm、体宽(17.70±0.33)cm时达到产卵量预期的峰值,随着体重等指标的进一步增加,产卵量有小幅度的回落。  相似文献   

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从患“红脖子病”甲鱼体分离到虹彩病毒   总被引:12,自引:2,他引:12  
从深圳某甲鱼场患“红脖子病”的甲鱼体内分离到一株病毒。该病毒于15~30℃能在CO、FHM、CK、GCK等细胞中增殖,其中在CO细胞中的滴度最高。最适复制温度为25~30℃,此时2dCPE可在90%以上。在感染病毒的CO细胞单层上覆盖琼脂后2~3d能形成直径1~1.5mm的空斑。病毒对氯仿、pH3、pH9敏感,DNA抑制剂(IUDR)能抑制其复制过程,提示该病毒为有囊膜的DNA病毒。病毒提纯后经负染电镜观察,可见到大量直径120~160nm的有囊膜的球形颗粒,放大后呈多面体。在感染病毒的细胞切片中,可见到六角形的病毒颗粒存在于细胞核和细胞质内,并可见到病毒从细胞膜获得囊膜。以上结果提示该病毒属虹彩病毒科,建议命名为甲鱼虹彩病毒(STIV)。人工感染试验显示,该病毒对甲鱼有中等毒力,与传染性胰坏死症病毒(IPNV)、草鱼出血病病毒(GCRV)、大马哈鱼呼肠孤病毒(CSV)在血清学上没有相关性。  相似文献   

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Intestinal obstruction by radiolucent foreign bodies is common in sea turtles. The absence of clinical signs and the extended fasting periods in these animals means that intestinal obstructions may not be diagnosed early. Digestive tract radiographic contrast procedures were performed to evaluate the gastrointestinal transit time and intestinal obstructions in loggerhead sea turtles. Barium sulfate and nonionic iodinate contrast medium were used for radiographic contrast procedures. Contrast medium was administered via a gastric tube or into the colon. These procedures are often necessary and are useful to document intestinal obstruction and foreign objects. The diagnostic procedures were not easily performed in some turtles, but often they were adequate for the diagnosis.  相似文献   

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甲鱼“红脖子病”的研究   总被引:15,自引:0,他引:15  
对某甲鱼养殖场1987年2~6月流行的甲鱼“红脖子病”进行了流行病学、临床,病理学、病原学、血清学等项检查,证明该病是由嗜水气单胞菌嗜水亚种所引起,故命名为甲鱼嗜水气单胞菌感染。  相似文献   

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《Veterinary microbiology》2015,175(2-4):218-223
Herpesviruses are significant pathogens of chelonians which most commonly cause upper respiratory tract disease and necrotizing stomatitis. Herpesvirus infection was identified in two populations of captive Eastern box turtles (Terrapene carolina carolina) using histopathology and polymerase chain reaction (PCR) with DNA sequencing. Necrotizing lesions with eosinophilic to amphophilic intranuclear inclusion bodies were identified in the tissues of one hatch-year individual in January 2013, which was herpesvirus positive by PCR. A separate captive group of adults had an observed herpesvirus prevalence of 58% using PCR in July 2011. In these cases, a novel herpesvirus, Terrapene herpesvirus 1 (TerHV1), was identified and serves as the first herpesvirus sequenced in the genus Terrapene. Similar to the other herpesviruses of the Order Testudines, TerHV1 clusters with the genus Scutavirus of the subfamily Alphaherpesvirinae.  相似文献   

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To the best of our knowledge, limited pharmacokinetic information to establish suitable therapeutic plans is available for Hawksbill turtles. Therefore, the present study aimed to assess the pharmacokinetic features of tolfenamic acid (TA) in Hawksbill turtles, Eretmochelys imbricata, after single intravenous (i.v.) and intramuscular (i.m.) administration at dosage 4 mg/kg body weight (b.w.). The study (parallel design) used 10 Hawksbill turtles randomly divided into equal groups. Blood samples were collected at assigned times up to 144 hr. The concentrations of TA in plasma were quantified by a validated liquid chromatography tandem mass spectrometry (LC-ESI-MS/MS). The concentration of TA in the experimental turtles with respect to time was pharmacokinetically analyzed using a noncompartment model. The Cmax values of TA were 89.33 ± 6.99 µg/ml following i.m. administration. The elimination half-life values were 38.92 ± 6.31 hr and 41.09 ± 9.32 hr after i.v. and i.m. administration, respectively. The absolute i.m. bioavailability was 94.46%, and the average binding percentage of TA to plasma protein was 31.39%. TA demonstrated a long half-life and high bioavailability following i.m. administration. Therefore, the i.m. administration is recommended for use in clinical practice because it is both easier to perform and provides similar plasma concentrations to the i.v. administration. However, further studies are needed to determine the clinical efficacy of TA for treatment of inflammatory disease after single and multiple dosages.  相似文献   

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中华鳖出血性肠道坏死症病原及其药敏性   总被引:9,自引:0,他引:9  
从中华鳖出血性肠道坏死症患病个体的血液、脾脏、肝脏及肠壁中共分离到 5株细菌 ,经人工感染、毒力测试 ,鉴定分离菌株为嗜水气单胞菌 (Aeromonas hydrophila)、豚鼠气单胞菌 (A.Caviae)和类志贺邻单胞菌 (Plesiomonasshigelloides)。药敏试验结果表明 ,此 3种致病菌对新霉素、丁胺卡那、呋喃妥因均高度敏感。  相似文献   

20.
中华鳖六株病原菌的分类鉴定及其中草药拮抗研究   总被引:5,自引:0,他引:5  
为提高养殖质量、达到无公害中华鳖的生产,本研究对来源于不同地域的中华鳖常见疾病的六株致病菌(WG985、TL97427、FS954、JS967、SG978、SS978)菌株进行了分类鉴定与中草药抑菌敏感性测试.结果表明4株为温和气单胞菌,2株分别为嗜水气单胞菌和豚鼠气单胞菌,6菌株对健康中华鳖均有较强毒力.药敏测定显示各菌株对中草药的拮抗作用基本一致,对大多数药物均表现不敏感,仅就地锦草、五倍子等个别药物敏感,但彼此间仍存有一定差异.  相似文献   

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