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1.
[目的 ]了解广州市活禽批发市场禽流感病毒的污染情况,为广州市制定有针对性的防控措施提供技术支持。[方法 ]2015年2月—2016年1月,按照随机抽样原则,对广州市2个活禽批发市场进行调查,每月采集1次棉拭子样品(共3 090份),并对所采集的样品进行禽流感病毒荧光RT-PCR检测。[结果 ]在该期间内,2个活禽批发市场的样品禽流感总阳性率为52.50%(1 625/3 090);禽流感病毒检出阳性率较高的月份是4月和9—12月,较低的是6—8月;水禽的禽流感感染风险是鸡的1.6倍,环境中存在禽流感病毒的风险是鸡的1.39倍;各月份中,1月、3月、11月和12月环境样品的禽流感病毒阳性率显著高于家禽样品(P0.01),而6月份家禽样品的禽流感病毒阳性率显著高于环境样品(P0.01);来自广东省内(广州市除外)的活禽样品禽流感病毒阳性率最高,为49.31%,来自外省市的禽流感通用型阳性率最低,为31.43%(P0.01)。[结论 ]调查发现,广州市活禽批发市场的禽流感高发期为春夏及秋冬交替季节;活禽批发市场中的水禽和批发市场环境是禽流感病毒流行的关键危险因素。调查结果提示,今后要加强春夏和秋冬交替季节的禽流感防控工作,加强对批发市场水禽的禽流感防控和批发市场环境的清洗、消毒,以切实降低活禽批发市场的禽流感污染水平。  相似文献   

2.
为掌握乌鲁木齐市某活禽交易市场的活禽交易点及市场外环境中禽流感病毒污染情况及其流行规律,制定科学有效的活禽交易市场的日常管理及消毒措施。消毒休市前后分别在相同活禽交易点及活禽交易市场车辆通道采集棉拭子样品。采用实时荧光定量(RT-PCR)方法检测通用型禽流感病毒核酸污染状况,比较活禽交易市场的关键风险点消毒休市前后消毒效果的差异情况。结果表明:该活禽交易市场的消毒技术规范及休市制度对禽流感病毒的作用较为明显,通用型禽流感病毒核酸阳性率从23.9%降至5.4%。  相似文献   

3.
为了解湖南省岳阳市活禽批发市场禽流感病毒污染情况,提升禽流感防控针对性,2018年1月~12月岳阳市在岳阳楼区枫桥湖蛋禽批发市场开展了活禽交易市场禽流感专项监测,累计检测600份禽拭子及120份环境拭子。共检测出H5亚型AIV阳性139份、H7亚型AIV阳性25份。结果表明家禽交易市场长期处于禽流感病毒污染状态。为降低疫情发生和人感染风险,建议加强活禽市场管理,特别要做好市场定期休市和清洗消毒工作。  相似文献   

4.
[目的]监测活禽零售市场休市前后家禽和环境中禽流感病毒的分布和波动状况,评估定期休市干预效果。[方法]在2015年9月28日至11月11日的3轮休市期间,从云南省选取4个有代表性的活禽零售市场,于休市的前1天、休市当日及休市后1天(复市当日)、3天、5天、10天,采集家禽喉气管、咽喉/泄殖腔棉拭子和环境样品,连续进行3轮采样,采用甲型流感病毒通用型RT-PCR方法,检测样品中的病毒核酸,开展禽流感病毒分布动态监测和休市干预效果评估。[结果]不同市场、不同休市轮次的休市干预效果参差不齐;禽流感病毒在鸡鸭混杂销售的市场中呈现持续分布,而在仅有鸡销售的市场中呈间断或零星分布;鸭群样品阳性率高于环境样品和鸡群样品阳性率。[结论]研究结果提示:鸭群对活禽零售市场的禽流感病毒传入、扩散和持续分布发挥着极其重要的作用;活禽零售市场的休市干预效果受多种因素影响,只有科学规范实施休市制度和严格执行监管措施,才能获得良好的休市干预效果。  相似文献   

5.
2017年1月11日至17日,江门地区活禽批发市场休市7d,我们通过对辖区内3个活禽批发市场休市前、中、后的禽流感病毒进行监测检测,结果显示,无论是哪种采样环境,还是休市前、中、后的样品,均未检出H7亚型流感病毒核酸,H5亚型也是极少数量,只在休市后的屠宰车间样品中检出1份H5亚型。活禽批发市场实行临时休市活禽零存栏和彻底清理消毒等措施可降低档口环境的禽流感病毒污染,是科学有效的,但是活禽批发市场休市前后的屠宰车间和活禽咽/泄殖腔样品中的A型流感、H9亚型阳性率无统计学差异,说明休市前后活禽屠宰环节也存在禽流感病毒污染与传播的风险,要进一步加强活禽屠宰和养殖等环节的防控措施。防控禽流感特别是防止人感染H7N9亚型流感单单在活禽市场环节采取措施仍不够,应综合多方落实措施,才能实现更好地防控。  相似文献   

6.
[目的 ]评估活禽零售市场"零存栏"措施对禽流感的防控效果。[方法 ]选择广州市某区2个有代表性的活禽零售市场,相隔1周进行"零存栏"与非"零存栏"交替干预。整个研究为期4周,每周采集3天次相应的环境样品和活禽样品并进行通用型禽流感病毒荧光RT-PCR的检测。[结果 ]每周环境样品中禽流感病毒阳性率依次为50.7%、16.7%、35.3%和66.7%。"零存栏"与"非零存栏"期间环境样品禽流感病毒阳性率分别为41.67%和43.00%,没有统计学差异。[结论 ]本研究为国内首次较为系统地对目前活禽零售市场实施的"零存栏"措施进行评估的干预性流行病学调查。评估认为在本研究的框架下,活禽零售市场是否实施"零存栏"措施,对减少活禽零售市场禽流感病毒污染状况的作用不大,须采取降低家禽养殖场的禽流感病毒感染率、减少活禽在运输与交易(活禽批发市场等)环节的感染几率、杜绝活禽售卖等综合的防控措施。  相似文献   

7.
[目的]了解广州市活禽农贸市场禽流感病毒的分布和流行情况,科学制定禽流感的防控措施,于2013年3-11月对广州市91个次的活禽农贸市场进行了禽流感监测,[方法]调查共采集了1064份活禽口咽/泄殖腔棉拭子和环境拭子,并用荧光RT-PCR方法进行了检测,[结果]发现通用型、H7N9和H9亚型禽流感阳性率分别为46.05%、0.09%和19.83%。其中,鸭棉拭子样品中通用型禽流感阳性率最高,达到了58.14%,其次为鸡棉拭子、鹅棉拭子和环境棉拭子样品,分别为46.28%、42.85%和35.19%;H9亚型禽流感阳性率最高的是鸡源样品,达到了28.88%,其次为鸭棉拭子和环境棉拭子样品,分别为12.56%和5.58%;仅从1份鸡棉拭子样品中检测到H7N9亚型禽流感病毒阳性。[结论]本次调查显示,广州地区活禽农贸市场存在较为严重的禽流感污染情况,需立即采取有效的防控措施以降低本地区发生禽流感以及人感染禽流感的风险。  相似文献   

8.
为了解冬季新疆乌鲁木齐市活禽市场交易禽类及市场外环境中禽流感病毒污染情况及其流行规律,对某交易市场内的鸡、鸭、鹅、鸽等活禽,采集85份活禽全血样本和143份棉拭子样本(103份活禽泄殖腔/咽喉棉拭子、40份环境棉拭子),分别采用血清学和实时荧光定量(rRT-PCR)方法,检测禽流感免疫抗体和禽流感病毒核酸,并对核酸阳性样品进行病毒亚型确定。结果显示:85份全血样本中,H5(Re-11)株免疫抗体合格32份,合格率为37.6%;H5(Re-12)株免疫抗体合格34份,合格率为40%;H7(Re-2)株免疫抗体合格46份,合格率为54.2%。143份棉拭子样本中,禽流感病毒核酸阳性36份,阳性率为25.17%。其中:活禽棉拭子阳性29份,阳性率为28.15%;交易区外环境棉拭子阳性7份,阳性率为17.5%。36份禽流感病毒核酸阳性样本中,H5亚型3份,阳性率为8.33%;H9亚型22份,阳性率为61.11%;未定型11份,阳性率为30.56%。结果表明:该市场交易活禽流感病毒感染率较高,且强制免疫的H5、H7亚型禽流感免疫抗体合格率较低,存在疫情发生和散播风险。结果提示,应加强活禽交易市场的消毒与管理,降低禽流感病毒通过活禽交易市场传播的风险。  相似文献   

9.
疫情     
《饲料广角》2014,(7):5-6
江苏洪泽活禽交易市场每月休市2—3d防控疫情 日前.洪泽县出台了禽类交易和活禽宰杀市场休市制度,每月休市2—3d,以进一步加强禽类交易和活禽宰杀市场的管理.有效防控高致病性禽流感等重大动物疫病的发生和流行。  相似文献   

10.
为了解云南省玉溪市活禽交易市场禽流感病毒污染情况,2021年对该市8个县(市、区)活禽市场进行采样检测。全年共采集63场次1 455份棉拭子样品(禽咽喉/泄殖腔双拭子860份、环境拭子317份、运输工具拭子278份),采用荧光RT-PCR方法进行流感病毒A型、H5/H7/H9亚型流感病毒核酸检测。共检出A型流感病毒核酸阳性435份,未检测到H5和H7亚型病毒核酸阳性;除检出7份未分型阳性样品外,在44场次检出H9亚型病毒核酸阳性428份,场次阳性率为69.84%,样品阳性率为29.42%,其中活禽、环境、运输工具样品阳性率分别为32.56%、25.87%、23.74%,差异具有统计学意义(P<0.05)。一年四季均能检测到H9亚型病毒阳性,但3—6月份样品阳性率低于年均阳性率,1、2、7、10、12月样品阳性率明显高于其他月份,各月份间样品阳性率差异具有统计学意义(P<0.01)。各县(市、区)的场次阳性率存在差异(P<0.05),但样品阳性率差异无统计学意义(P>0.05)。结果表明:玉溪市活禽市场H5、H7亚型高致病性禽流感病毒的污染状况得到有效控制,但H9亚型禽流感病毒污染面依然广,污染率较高;不同月份间阳性率差异明显,但季节性差异缩小。结果提示,应继续加强活禽市场H9亚型禽流感病毒污染监测,同时强化活禽市场的检疫监管,严格执行消毒和休市制度,防止活禽市场禽流感病毒传入养殖环节。  相似文献   

11.
Heartworm in dogs in Canada in 1983   总被引:6,自引:6,他引:0       下载免费PDF全文
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.

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12.
13.
Heartworm in dogs in Canada in 1985   总被引:3,自引:3,他引:0       下载免费PDF全文
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

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14.
Heartworm in dogs in Canada in 1988   总被引:1,自引:1,他引:0       下载免费PDF全文
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).  相似文献   

15.
Heartworm in dogs in Canada in 1984   总被引:4,自引:4,他引:0       下载免费PDF全文
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

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16.
17.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium.  相似文献   

18.
Heartworm in dogs in Canada in 1989   总被引:1,自引:1,他引:0       下载免费PDF全文
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

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19.
Heartworm in dogs in Canada in 1987   总被引:1,自引:1,他引:0       下载免费PDF全文
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).  相似文献   

20.
Heartworm in dogs in Canada in 1986   总被引:2,自引:2,他引:0       下载免费PDF全文
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.

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