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1.
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2.
Summary

Photosynthesis of tomato plants (Lycopersicon esculentum (L.) Mill. cv. F144) was studied under conditions of CO2 supplementation and salinity. The purpose of the study was to elucidate the mechanisms underlying the effects of salinity on the acclimation of tomato plants to CO2 supplementation. Plants were grown under either low (355.mmol mol–1) or elevated (1200.6.50 mmol mol–1) CO2 and were irrigated with low concentrations of mixed salts. The highest salinity level (E.C. 7 dS m–1) was that used to produce quality tomatoes in the Negev highlands, in Israel. During early development (three weeks after planting), the net photosynthetic rate of the leaves was much higher under elevated CO2, and other than a slight decrease in quantum yield efficiency as measured by fluorescence (DF/F 9 m ), no signs of acclimation to high levels of CO2 were apparent. Clear acclimation to high CO2 concentration was evide t ten weeks after planting when the net photosynthetic rate, photosynthetic capacity, and carboxylation efficiency of leaves of non-salinized plants were strongly suppressed under elevated CO2. This was accompanied by reductions in carboxylation efficiency, Rubisco activity and PSII quantum yield, and an increased accumulation of leaf soluble sugars. The reduction in photosynthetic capacity in the high CO2 plants was less in plants grown at the highest salinity level. This was correlated with an increase in the PSII quantum yield parameters (Fv/Fm) and DF/F 9 m ) but not with Rubisco activity which was affected by the CO2 treatments only. These results explain the effects of high CO2 on yields in tomatoes grown at high levels of salt (Li et al., 1999).  相似文献   

3.
Changes in light intensity have a particular effect on the photosynthetic apparatus. Most of the studies on light acclimation in higher plants have focused on the effects of fixed light intensity. Few works deal on sudden changes in light intensity due to pruning for long periods of time. Pruning the bent shoots in roses (cv. Grand Gala) can modify the light interception in internal leaves and change their photosynthetic reactions. Before pruning, internal leaves were acclimated to low light intensity and after pruning, internal and external leaves received the same light intensity. The aim of this work was to find out how the photosynthetic light reaction, measured by chlorophyll (Chl) a fluorescence in internal and external leaves of arched shoot could be modulated by light changes. Other parameters described as light dependent were also measured: nitrate reductase (NR) activity, NH4+, sucrose and starch levels. Internal and external leaves in rose have shown a high plasticity, rapid and dynamical acclimation, in response to changes in incident sunlight produced by pruning, that can be explained by Chl a fluorescence parameters. The modified NR activity, NH4+, sucrose and starch levels were difficult to associated with light intensity changes, and their modulation could be the response to long-time light acclimation.  相似文献   

4.
Summary

Shoot growth attributes, leaf anatomical changes and net photosynthetic rates were determined in young coffee plants fertilized at high and low levels and subjected to shade or full sunlight. High nitrogen supply increased plant height irrespective of the light level imposed during growth, and promoted a larger leaf area in plants of the shade treatment. Specific leaf weight was 15% higher in plants grown in full sunlight than in shaded plants, at both nitrogen levels. The number of leaves developed on the orthotropic stem increased significantly under full irradiance and high nitrogen availability. Leaves were 11% thicker ih unshaded plants than in shaded ones, because of the increased size of the palisade and spongy parenchyma tissues. More thylakoids per granum and more grana per chloroplast were observed in shade-grown plants, mainly in those given high nitrogen treatment. Total nitrogen content expressed on a leaf-area basis was higher in sun plants supplemented with nitrogen, whereas chlorophyll a and b and protochlorophyll contents increased in shade-grown plants. Net photosynthetic rate in high nitrogen plants reached 7.19 µmol CO2 m?2 s?1 in the full irradiance treatment, and 5.46 µmol CO2 m?2 s?1 in shaded plants. Maximum net photosynthetic rates in the low nitrogen plants were 5.28 and 4.90 µmol CO2 m?2 s?1 in sun and shade plants, respectively. Increased photosynthetic light saturation was observed in high irradiance plants (628 µmol m?2 s?1) relative to shade plants (359 µmol m?2 s?1) in the high nitrogen treatment. The same pattern was observed in low nitrogen plants. The apparent quantum yield of shade acclimated plants was 14% higher than in those grown in full sunlight. Our results indicate that coffee may be classified as a facultative shade species, exhibiting features of sun adapted plants coupled with shade acclimation attributes, this fairly high adaptive capacity to shade:sun transition being strongly influenced by the level of nitrogen nutrition given to the plants.  相似文献   

5.

Background

Freezing tolerance is an important factor in the geographical distribution of plants and strongly influences crop yield. Many plants increase their freezing tolerance during exposure to low, nonfreezing temperatures in a process termed cold acclimation. There is considerable natural variation in the cold acclimation capacity of Arabidopsis that has been used to study the molecular basis of this trait. Accurate methods for the quantitation of freezing damage in leaves that include spatial information about the distribution of damage and the possibility to screen large populations of plants are necessary, but currently not available. In addition, currently used standard methods such as electrolyte leakage assays are very laborious and therefore not easily applicable for large-scale screening purposes.

Results

We have performed freezing experiments with the Arabidopsis accessions C24 and Tenela, which differ strongly in their freezing tolerance, both before and after cold acclimation. Freezing tolerance of detached leaves was investigated using the well established electrolyte leakage assay as a reference. Chlorophyll fluorescence imaging was used as an alternative method that provides spatial resolution of freezing damage over the leaf area. With both methods, LT50 values (i.e. temperature where 50% damage occurred) could be derived as quantitative measures of leaf freezing tolerance. Both methods revealed the expected differences between acclimated and nonacclimated plants and between the two accessions and LT50 values were tightly correlated. However, electrolyte leakage assays consistently yielded higher LT50 values than chlorophyll fluorescence imaging. This was to a large part due to the incubation of leaves for electrolyte leakage measurements in distilled water, which apparently led to secondary damage, while this pre-incubation was not necessary for the chlorophyll fluorescence measurements.

Conclusion

Chlorophyll fluorescence imaging is an alternative method to accurately determine the freezing tolerance of leaves. It is quick and inexpensive and the system could potentially be used for large scale screening, allowing new approaches to elucidate the molecular basis of plant freezing tolerance.  相似文献   

6.
Summary

Dry-matter accumulation and partitioning in plants of Zantedeschia ‘Best Gold’ were quantified under a range of temperature and photosynthetic photon flux (PPF) regimes using plant growth analysis. Initiation of tuber growth did not require an obligate environmental trigger. Under both PPF regimes, relative growth rate of the tuber (RGRt) increased linearly with increasing temperature (13 to 28°C) up to a maximum at 28°C, with a base temperature of 3.2 ± 1.1°C. Optimum temperature for tuber growth was found to be PPF dependent, but maximum tuber dry mass was calculated as occurring under low PPF (348 µmol m–2 s–1) at 24.5 ± 0.1°. Mechanisms of acclimation under both PPF regimes suggested that tuber growth was principally source limited. Source limitation was expressed either in terms of: 1) enhanced inter-sink competition for assimilates, as occurred under the low PPF regime, where leaf area development was in direct competition with tuber growth (RGRt) or, 2) efficiency of dry-matter accumulation by the leaf area present, as occurred under the high PPF regime, where large increases in RGRt were correlated with increased net assimilation rate (NAR). Use of the daily increment of dry matter into tuber tissue (TMP) provided a more sensitive measure of short-term changes in partitioning than the conventionally used term, harvest index.  相似文献   

7.
A/Ci and photosynthetic light response curves for gas exchange characteristics were measured for spur leaves of 25-year-old ‘Golden Delicious’ and ‘Granny Smith’ apple trees (Malus domestica Borkh.) to investigate their acclimation capacity to a shade-to-sun transition in a commercial apple orchard. Spur leaves of both cultivars adapted to summer-pruning within 2 weeks, regardless of the time of the season at which pruning was conducted. There were no significant differences between the spur leaves of later pruned trees and corresponding leaves on trees that were continuously pruned during the summer period in terms of net photosynthetic rate and carboxylation efficiency, for both cultivars. The shape of the photosynthetic light response curves also proved the acclimation potential of both cultivars to shade-to-sun transitions. The rapid increase of the chlorophyll a/b ratio after pruning indicated that the acclimation of the photosynthetic system to avoid over-excitation of the photosynthetic reaction centers occurred within 1 week. Nevertheless, leaf dry mass per leaf area was intermediate between ‘continuous summer-pruning’ and ‘no-pruning’ treatments for leaves on later pruned trees, indicating that the acclimation to shade-to-sun transitions was not perfect in either cultivar. The present data also support the hypothesis that nitrogen, phosphorus, calcium, and magnesium are distributed to leaves growing under the highest photosynthetic photon flux density in order to maximize photosynthesis.  相似文献   

8.
The use of related species to integrate important traits into cultivated crops is a common practice in plant breeding. Gaura coccinea and, potentially its derived species Gaura drummondii, could be donor species for introgressing cold tolerance into non-hardy G. lindheimeri. However, cold tolerance and acclimation has not been studied in these species, so protocols for determining these traits are required. The objectives of this study were to determine the relative cold tolerance of G. coccinea, G. drummondii and whether short day photoperiod is involved in cold acclimation. G. drummondii from Texas, USA and G. coccinea from Minnesota and Texas, USA were subjected to freezing tests using whole plant or electrolyte leakage after natural acclimation or non-acclimation conditions. Minnesota genotypes were able to withstand colder temperatures (−12 °C) than Texas genotypes (−9 °C). Acclimation capacity was determined for whole plant and electrolyte leakage assays using three different plant organs—stem, crown, and rhizome. Underground rhizomes were the best predictors of cold tolerance, however they were difficult to obtain. Stem sections of G. drummondii (Texas) and G. coccinea (Minnesota and Texas) were used to determine a shift in acclimation under short days. The Minnesota G. coccinea genotypes demonstrated greater cold tolerance after 3 weeks of short days while Texas G. coccinea, G. drummondii genotypes did not change even after 5 weeks.  相似文献   

9.
Summary

Quantitative trait loci (QTL) mapping and marker development are essential steps in any molecular breeding programme. In this study, QTL for photosynthetic pigment concentration were identified using a doubled-haploid (DH) population of 112 lines derived from an F1 hybrid of Pak Choi, ‘Shulv’, obtained from a cross between Brassica rapa lines SW-13 and SU-124 through microspore culture. QTL mapping was carried out using a modification to the map of ‘Shulv’. Alignment of the linkage groups was carried out with those of reference B. rapa maps, allowing the assignment of these groups as A01 – A10. In total, 11 QTL were associated with photosynthetic pigment concentration and identified on linkage groups A05, A08, and A10. The maximum phenotypic variance for a single QTL was 38.0%. Markers closely linked to these QTL could assist in the development of Pak Choi cultivars with increased yield, due to having improved photosynthetic capabilities.  相似文献   

10.
The aim of this work was to explore leaf characteristics underlining the difference in the sensitivity of pea cultivars (cv. Kelvedon, Douce and Lincoln) to Fe deficiency. Plants were grown in a greenhouse under controlled conditions in continuously aerated solution. Three treatments were used: 30 μM Fe (+Fe), 0 μM Fe (−Fe); direct deficiency and 30 μM Fe + 10 mM NaHCO3 (+Fe+Bic); indirect deficiency for 12 days. Growth parameters, iron status, potassium content, chlorophyll fluorescence and photosynthetic capacity were studied. Our results showed that Fe deficiency led to a significant decrease of chlorophyll index (SPAD readings) and bivalent iron content in all Pisum sativum cultivars. The lower reduction was observed in Fe-deficient plants of Kelvedon and Douce. In addition, shoot length and whole plant dry weight were not affected by Fe deficiency in the latter cultivars. Both tolerant cultivars showed higher accumulation of potassium content in their leaves compared with the sensitive one. Moreover, both chlorophyll fluorescence ratios (Fv/Fm and Fv/F0) were significantly decreased in all cultivars under both Fe deficiency treatments. The photosynthetic electron transport activity was reduced in the sensitive cultivar, especially in the absence of iron. The adverse effect of bicarbonate-induced Fe deficiency on the above mentioned parameters were more pronounced than that of the direct one. The capacity of both tolerant cultivars to preserve adequate chlorophyll synthesis, photosynthetic capacity and plant growth under iron-limiting conditions is related to the suitable nutrition of their leaves in ferrous iron, due to (at least partially) their higher potassium content.  相似文献   

11.
The introduction of Aglaonema commutatum as an ornamental plant has been an empirical process. The success reached seems due to the capacity of the species to grow in very low light intensities for long periods. The results obtained have shown: (1) the light- saturated photosynthetic rate was very low even for shade plants (0.7-1.0 (μmol m-2s-1), (2) growth at the lowest irradiance did not stop despite a negative net CO2 exchange. The growth of new leaves was sustained by reserves stored in the cutting, (3) the plants grown at the lowest irradiances showed a decrease in light-saturated photosynthetic rate and a change in dry-matter partitioning with poor growth of root systems, and (4) the responses were influenced more by light intensity than by light quality.  相似文献   

12.
Summary

Nitric oxide (NO) is an active molecule involved in many physiological functions in plants. To characterise the roles of NO in the tolerance of eggplant (Solanum melongena L.) to salt stress, the protective effects of exogenous sodium nitroprusside (SNP), a donor of NO, applied at different concentrations (0, 0.05, 0.1, or 0.2 mM), on plant biomass, photosynthesis, and anti-oxidant capacity were evaluated. The application of SNP alleviated the suppression of growth in eggplant under salt stress, as reflected by a higher accumulation of biomass. In parallel with growth, the application of SNP to salt-stressed plants resulted in enhanced photosynthetic parameters such as the net photosynthetic rate (Pn), stomatal conductance (gs), transpiration rate (Tr), and intercellular CO2 concentration (Ci), as well as an increased quantum efficiency of PSII (Fv/Fm), efficiency of excitation capture of open PSII centres (Fv’/Fm’), quantum yield of PSII ( psii) and photochemical quenching coefficient (qP). Furthermore, exogenous SNP also reduced significantly the rate of production of O2? – radicals and the concentrations of malondialdehyde (MDA) and H2O2. It also increased the activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) in eggplant leaves grown under salt stress. The results indicated that the protective effects of NO against salt stress in eggplant seedlings were most likely mediated through improvements in photosynthetic performance and the stimulation of anti-oxidant capacity.  相似文献   

13.
Summary

The dynamic responses in shoot growth of three pot plants ofHedera helix L. ‘Mein Herz‘ in relation to changes in photosynthetic photon flux density (PPFD) is identified. In the experiment a two-level strategy of PPFD to the plants was used. The current level of PPFD on any day was either high or low, and was determined using a pseudo random binary signal (PRBS) both to avoid any correlations between the PPFD and other variables, and to ensure that the dynamic characteristics of the plant growth were present in the data. For each of the three pot plants a discrete dynamic model of growth responses to PPFD was estimated, and an agreement between the parameters of the three models was observed. The results show that not only is the current PPFD level of the current day related to the daily increase in fresh weight, but growth is also a function of the PPFD level of the previous days. With respect to the previous days the effects of PPFD can be described as an exponential decay where from one day to the previous day the effect of PPFD is reduced to 67%, 66% and 80% respectively for the three pot plants in the experiment.  相似文献   

14.
Summary

Dehydrins are a group of plant proteins which respond to any type of stress that causes dehydration at the cellular level, such as cold and drought stress. Previously, three dehydrins of 65, 60, and 14.kDa were identified as the predominant proteins present in cold acclimated blueberry (Vaccinium corymbosumLinn.) floral buds. Levels were shown to increase with cold acclimation and decrease with deacclimation and resumption of growth. In the present study, to determine if dehydrins are induced in other organs in response to low temperature treatment (48C) and in response to drought, accumulation of dehydrins was examined in leaves, stems, and roots of two cultivars and one wild selection (a V. corymbosum cultivar, a V. ashei Reade cultivar, and a V. darrowi Camp selection) of blueberry by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting. Cold treatment involved placing plants in a cold room maintained at 48C for five weeks; drought stress was imposed by withholding water from potted, greenhouse-grown plants for 34.d. Relative water content (RWC) of shoots was determined periodically throughout the drought treatment. Dehydrins accumulated with both cold and drought stress but their molecular masses varied depending upon blueberry species. Dehydrins accumulated to higher levels in stems and roots than in leaves with cold stress and to higher levels in stems than in either roots or leaves with drought stress. Furthermore, cold treatment combined with dark treatment induced higher levels of dehydrins than cold treatment combined with a 10.h light/14.h dark photoperiod, suggesting that dehydrins may be responsive to changes in photoperiod as well. In the cold-stress experiment, the level of dehydrin accumulation was correlated with expected level of plant cold hardiness in the three genotypes. In the drought stress experiment, dehydrins accumulated prior to significant changes in RWC, and dehydrin levels did not appear to be closely correlated with RWC either among or within genotypes.  相似文献   

15.
Summary

Ryegrass (Lolium perenne L., cv. ‘Victorian’) seedlings from seed pre-treated by low-dose gamma irradiation (LDG) were exposed to 6.5 kJ m–2 d–1 UV-B radiation under controlled environmental conditions. The high level of UV-B radiation caused serious plant injury, such as accelerated senescence, growth inhibition and lipid oxidation in cell membranes, but UV-B-induced injury was alleviated significantly by LDG-pretreatment, in particular by 20 Gy and 30 Gy (1 Gy = 1 J kg–1). Both ascorbic acid and UV-absorbing compounds were increased by LDG-pretreatment, and the latter were further increased 2-fold by UV-B irradiation. During UV-B exposure, the levels of photosynthetic pigments remained high in seedlings from seed pre-treated with 30 Gy. We suggest that increased plant tolerance to UV-B damage, caused by LDG-pretreatment, may be due, in part, to higher contents of ascorbic acid and UV-absorbing compounds, and the ability to maintain high levels of photosynthetic pigments. The possible implications of these observations are discussed.  相似文献   

16.
Summary

In this paper, the floral biology and pollination ecology of Salvia splendens are described. All flower characteristics (red corolla, large tubular flowers with abundant but dilute nectar) indicate that S. splendens is adapted to hummingbird pollination. Honeybees, however, were also found to be good pollinators of this plant. Apis mellifera was equally effective in pollinating S. splendens as hand pollination and open pollination, resulting in a 300% increase in seed set compared with bagged control plants. The much smaller stingless bee Tetragonisca angustula was not an effective pollinator of these flowers since during visitation its body failed to touch the stigma. Although pollination seemed not to be the only limiting factor in seed production, external pollinators enhance seed production in S. splendens, and Apis mellifera is an effective, commercially available pollination agent of this economically important ornamental plant.  相似文献   

17.
Summary

Physical factors changing the acclimation and deacclimation processes that lead to cold (frost) tolerance and susceptibility in Rhododendron cv. Hatsurgiri are reported. Interactions between photoperiod and temperature which encouraged acclimation were studied by exposing detached leaves 0°C to –10°C, and assessing resulting injury using ion efflux and visual scoring. Combinations of short days (8 h) and exposure to 5°C resulted in the greatest cold tolerance. Exposure to long days (18 h) or 20°C reduced the cold tolerance of leaves. Application of each environmental factor, separately, increased cold tolerance compared with control tissues. When photoperiods were maintained at 12 h, exposing leaves to 5°C during the dark period enhanced cold tolerance compared with a 20°C dark period. Continuous 5°C applied during both light and dark periods encouraged greatest cold tolerance. Deacclimation from the cold tolerant state was accelerated by both higher temperature and longer photoperiods, but temperature was predominantly the more significant factor. Application of short days combined with low temperatures significantly delayed deacclimation. But even in this environment cold tolerance decreased over time, possibly through the action of endogenous annual rhythms. Cold tolerance changed with calendar time even when external environmental conditions were maintained at constant values and plant morphology was apparently unchanged.  相似文献   

18.
Summary

Trichoderma harzianum strain T-22 (T22) is one of the most effective strains of this fungus that is able to colonise the roots of most plant species across a wide range of soil types. This fungus is used as a biocontrol agent during crop production, and for the improvement of the rooting and acclimatisation phases in plant nurseries. In vitro-cultured shoots of GiSeLa6® (Prunus cerasus P. canescens) and of GF677 (P. amygdalus P. persica), two important Prunus varieties used as commercial rootstocks, were inoculated with T22. The results showed that early inoculation of the fungus (at the stage of shoot transfer to root-inducing medium) seriously damaged both GiSeLa6® and GF677 plants; whereas, following later inoculation (7 d after shoot transfer to root-inducing medium), the plants survived and showed significant increases in shoot growth and root development. In particular, root lengths in GiSeLa6® and GF677 plants increased by 180% and 136%, respectively, compared to non-inoculated controls. Microscopic analysis revealed T22 hyphae spreading on the root surface in GiSeLa6® (fungus colonisation frequency = 20%), but not in GF677 roots. Our results demonstrate that the application of T22 during the rooting phase resulted in greater shoot lengths, as well as increased numbers of leaves, roots, and stem diameters. These morphological characteristics could increase the quality and viability of nursery planting material and provide advantages during the plant acclimatisation phase.  相似文献   

19.
Summary

Miniature rose plantlets at the flower development stage were grown photo-autotrophically on MS medium and subsequently exposed to water deficits of –0.23, –0.32, –0.40, or –0.67 MPa osmotic potential ( s) for 14 d. The s in the culture medium was raised by increasing the concentration of mannitol, which caused abnormal floral development in terms of the flowering percentage and the number of flowers per plantlet, as well as delayed flowering. In vitro flowering and the number of flowers per plantlet declined significantly when miniature rose plantlets were exposed to water deficit stress at –0.40 MPa or –0.67 MPa. Reductions in growth, pigment degradation, chlorophyll a fluorescence, and net photosynthetic rate (Pn) were greatest in plantlets exposed to a water deficit stress of –0.67 MPa. This was particularly evident in the case of Pn, with a decline of 73.7% compared to non-stressed control plantlets. In contrast, proline levels increased in plantlets under water deficit stress, as proline performs a key role as an osmoprotectant under such conditions. The flowering stage in miniature rose plantlets is particularly susceptible to water deficit stress, which suppresses the development of reproductive organs. Knowledge of the responses to water deficit stress at the reproductive stage may be applied to identify effective indices for the selection of genotypes with increased tolerance to water deficit in miniature rose breeding programmes.  相似文献   

20.
SUMMARY

Gas exchange and photosystem II (PSII) activities in the leaves of 2-year-old ‘Bluecrop’ highbush blueberry (Vaccinium corymbosum) were monitored during water stress and subsequent re-irrigation to investigate the effects of the intensity of water stress on changes in photosynthetic characteristics. The blueberry shrubs were not irrigated for 3 to 5 weeks, then re-irrigated daily up to 8 weeks. The decrease in soil water potential during water stress caused a progressive decrease in leaf water potential. Soil water potentials decreased to -0.26 MPa and -0.34 MPa at 3 and 5 weeks, respectively, following water stress, but recovered following subsequent re-irrigation, while the soil water potential in daily-irrigated shrubs was maintained at over -0.13 MPa throughout the experiment. Chlorophyll concentrations decreased with an increasing duration of water stress. Chlorophyll concentrations in leaves on shrubs subjected to water stress for 5 weeks did not recover following re-irrigation, unlike those subjected to water stress for 3 weeks. The leaves on shrubs subjected to water stress for 5 weeks maintained lower levels of chlorophyll during reirrigation. The net rate of CO2 assimilation (An) decreased significantly with an increasing duration of water stress. Reirrigation reversed the decrease in An in leaves on shrubs subjected to water stress for 3 weeks. Stomatal conductance (gs) exhibited a similar pattern to An. The actual quantum yield of photosystem II (ΦPSII) and the electron transport rate (ETR) also decreased significantly with an increasing duration of water stress, although the Fv/Fm ratio was not affected. ΦPPSII and ETR values in the leaves on shrubs subjected to water stress for 5 weeks did not recover after reirrigation, unlike those subjected to water stress for 3 weeks. Non-photochemical quenching increased with an increasing duration of water stress, but subsequent re-irrigation did not reverse the increase. These results indicate that the timing of re-irrigation of water-stressed ‘Bluecrop’ highbush blueberry is critical in order to maintain their photosynthetic capacity. Among the photosynthetic characteristics measured, ΦPSII and ETR could be used as sensitive indicators to assess the physiological status of leaves of ‘Bluecrop’ highbush blueberry growing under water stress conditions.  相似文献   

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