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1.
蛋氨酸硒和纳米硒对波尔山羊种公羔生长及血液、组织硒含量的比较 总被引：2，自引：0，他引：2
[目的]为了研究纳米硒(nano-selenium)和蛋氨酸硒(methionine-selenitim)对种公羊羔羊生长发育以及血液、组织中硒的沉积和谷胱甘肽过氧化物酶活性的影响.[方法]试验选用体重相近的30只种用波尔山羊断奶公羔随机分3组,每组10个重复,预试2周,正试12周,分别饲喂基础日粮(对照组);基础日粮+0.3 mg/kg纳米硒;基础日粮+0.3 mg/kg蛋氨酸硒(以硒浓度计算).每隔4周采血样一次,试验结束时,每组随机屠宰羔羊3只测量组织硒浓度.[结果]不同的硒源和一定浓度的硒对公羔生长发育影响不显著(P>0.05);添加蛋氨酸硒和纳米硒的公羔组织器官,血清,全血硒含量以及肝脏谷胱甘肽过氧化物酶活性显著高于对照组(P<0.05),添加组之问差异不显著.[结论]统计结果表明,纳米硒和蛋氨酸硒与对照相比都能有效地增加血液、组织中硒的沉积,添加纳米硒组羔羊硒沉积和谷胱甘肽过氧化物酶活性稍高于蛋氨酸硒组,羔羊添加纳米硒更易于硒的吸收和沉积. 相似文献
2.
【目的】为了研究纳米硒(nano-selenium)和蛋氨酸硒(methionine-selenium)对种公羊羔羊生长发育以及血液、组织中硒的沉积和谷胱甘肽过氧化物酶活性的影响。【方法】试验选用体重相近的30只种用波尔山羊断奶公羔随机分3组,每组10个重复,预试2周,正试12周,分别饲喂基础日粮(对照组);基础日粮+0.3 mg/kg纳米硒;基础日粮+0.3 mg/kg蛋氨酸硒(以硒浓度计算)。每隔4周采血样一次,试验结束时,每组随机屠宰羔羊3只测量组织硒浓度。【结果】不同的硒源和一定浓度的硒对公羔生长发育影响不显著(P>0.05);添加蛋氨酸硒和纳米硒的公羔组织器官,血清,全血硒含量以及肝脏谷胱甘肽过氧化物酶活性显著高于对照组(P<0.05),添加组之间差异不显著。【结论】统计结果表明,纳米硒和蛋氨酸硒与对照相比都能有效地增加血液、组织中硒的沉积,添加纳米硒组羔羊硒沉积和谷胱甘肽过氧化物酶活性稍高于蛋氨酸硒组,羔羊添加纳米硒更易于硒的吸收和沉积。 相似文献
3.
为探讨不同硒源饲粮对鸡蛋品质、血液中硒沉积、鸡蛋中硒沉积的影响，试验选取400只340日龄健康状态良好的海兰褐蛋鸡，随机分为4组，每组5个重复，每个重复20只，分别饲喂基础日粮、基础日粮+0.5 mg/kg亚硒酸钠（试验A组）、基础日粮+0.5 mg/kg蛋氨酸硒（试验B组）、基础日粮+0.5 mg/kg纳米硒（试验C组），并于第7、14、28天测定蛋品质、鸡蛋和血液中的硒含量。结果显示：与对照组相比，饲粮中添加亚硒酸钠、蛋氨酸硒、纳米硒产蛋率分别提高0.82%、1.87%、2.47%，纳米硒提高产蛋效果显著（P <0.05）；各试验组日采食量分别提升0.84%、2.40%、2.75%，蛋氨酸硒和纳米硒组日采食量显著提高（P <0.05）；蛋鸡血液中第28天的硒含量分别提高44.88%、62.20%、127.17%(P <0.05)；鸡蛋中的硒沉积分别提高64.58%、87.50%、155.73%(P <0.05)。综上所述：在饲粮中添加不同硒源的饲粮均可以显著提高蛋鸡的生产性能，提高硒在血液和鸡蛋中的沉积效果，且纳米硒的提升效果更优于亚硒酸钠和蛋氨酸硒。 相似文献
4.
为了研究日粮中添加纳米硒对公羊睾丸发育的影响,试验选用体重、日龄接近的20只波尔山羊公羔羊随机分2组,1组日粮中添加0.3mg/kg的纳米硒(以硒计),另一组饲喂基础日粮,预试2周,试验期每月测量睾丸周径、长度和高度,每组阉割1只公羔,按照常规方法制作石蜡切片,观察睾丸组织结构。结果显示,3月龄时纳米硒组睾丸间质组织增生发育,4月龄时纳米硒组睾丸曲精细管中精原细胞开始发育分化;5月龄时曲精细管中可见大量的精子,而对照组间质组织以及曲精细管发育、精母细胞发育分化较纳米硒组缓慢。试验表明,日粮中添加一定浓度的纳米硒能够促进睾丸间质组织及曲精细管的发育,促进精母细胞的分化。 相似文献
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本试验旨在研究不同硒源对济宁百日鸡生产性能、鸡只健康状况、蛋中硒含量的影响,共设计2个试验。试验Ⅰ：25周龄济宁百日鸡200只随机分为4个组,每组5个重复,每个重复10只,对照组只饲喂基础日粮,3个试验组分别在基础日粮中添加亚硒酸钠、酵母硒与蛋氨酸硒三种不同含硒添加剂,硒源添加水平以饲料中硒元素0.4 mg/kg计算;预饲期为7 d,正式饲养试验持续56 d。试验Ⅱ：35周龄济宁百日鸡200只随机分为4个组,每组5个重复,每个重复10只,对照组只饲喂基础日粮,3个试验组分别在基础日粮中添加含0.4、0.6、0.8 mg/kg硒水平的蛋氨酸硒。预饲期为7 d,正式饲养试验持续28 d。试验结果表明：在本试验条件下,添加有机硒源组生产性能略高于无机硒源组和对照组,但各组间无显著差异（P>0.05）;饲料中添加有机硒源,比无机硒源更能促进济宁百日鸡鸡蛋中硒的沉积,且有机硒源中,蛋氨酸硒比酵母硒在鸡蛋富硒过程中转化效率更高;不同浓度的蛋氨酸硒均可显著提高济宁百日鸡鸡蛋中的硒含量（P<0.05）,综合比较以蛋氨酸硒在基础日粮中的硒水平浓度为0.6 mg/kg最适宜。 相似文献
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《中国畜牧杂志》2021,(3)
本试验旨在研究日粮中添加酵母硒和蛋氨酸硒对湖羊生长性能、羊肉品质和组织硒沉积的影响。选取33只4月龄育肥期公湖羊,随机分成对照组、酵母硒组和蛋氨酸硒组,对照组饲喂牧场基础日粮,酵母硒组和蛋氨酸硒组日粮中添加0.3 mg/kg硒,预试期1周,正试期8周。结果表明:与对照组相比,日粮中添加有机硒可显著提高湖羊日增重,但对干物质采食量等指标无显著影响;日粮中添加有机硒能显著增加肌肉红度(a*),并提高羊肉嫩度;补硒可显著提高湖羊肌肉、血液和内脏器官中的硒含量,且蛋氨酸硒组在湖羊器官中的沉积效果显著优于酵母硒组。可见,日粮中添加有机硒(0.3 mg/kg)可提高湖羊的生长性能和羊肉品质,提高肌肉和器官中硒含量。 相似文献
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为了研究日粮中添加纳米硒时公羊睾丸发育的影响.试验选用体重、日龄接近的20只波尔山羊公羔羊,随机分为两组,一组日粮中添加0.3 mg/kg的纳米硒(以硒计),另一组饲喂基础日粮,预试2周,试验期每月测量睾丸周径、长度和高度,每组阉割1只公羔,按照常规方法制作石蜡切片,观察睾丸组织结构.结果显示:3月龄时纳米硒组睾丸间质组织增生发育,4月龄时纳米硒组睾丸曲精细管中精原细胞开始发育分化;5月龄时曲精细管中可见大量的精子,而时照组间质组织以及曲精细管发育、精母细胞发育分化较纳米硒组缓慢.试验表明,日粮中添加一定浓度的纳米硒能够促进睾丸间质组织及曲精细管的发育,促进精母细胞的分化. 相似文献
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10.
高硒对产蛋鸡生产性能及蛋中硒沉积的影响 总被引：2，自引：0，他引：2
试验选用65周龄罗曼蛋鸡48只，随机分成4组，每组12只。在基础日粮含硒0.15mg/kg的基础上，添加硒酵母10mg/kg(以硒计）。实验预试期1周，正试期为4周。探讨日粮中高硒对产蛋鸡生产性能和蛋中硒沉积的影响，为日粮中硒含量的调控提供依据。结果表明，高硒日粮对试鸡的产蛋率和蛋重均无显著影响（P＞0．05），但可使蛋中硒含量极显著提高（P＜0．01）。 相似文献
11.
Maki HAYASHIDA Edgar A. ORDEN Emilio M. CRUZ Libertado C. CRUZ Tsutomu FUJIHARA 《Animal Science Journal》2004,75(5):423-430
The effects of Leucaena leucocephala leaf (LL) supplementation on selenium (Se) status of growing Philippine goats were determined in the present study. A total of 12 3–4 month old male goats with mean bodyweight of 8.8 kg was used. All animals received a basal diet comprised of different forages. Six of the animals were supplemented with LL (22.9% CP) while the remaining six animals were not supplemented (control). The rate of LL to the total forage diet offered to the LL‐supplemented animals was 25%. Bodyweight measurement, blood and forage sampling were carried out every 2 weeks during the 4 months of experiment. Selenium, sulfur, calcium, phosphorus, magnesium, copper and zinc concentrations were analyzed after wet digestion. Leucaena leaf seemed to have higher Se and calcium, but lower copper and zinc concentrations than was observed in grasses fed to the animals. Bodyweight of growing goats in LL‐supplemented group tended to be higher than that of control animals. Supplementation of LL resulted in high whole blood Se concentration of growing goats. In the last month of this experiment, whole blood in all supplemented animals contained higher Se concentration than the deficient level of 20 ng/mL, while Se concentration in the control group was slightly above the deficient level. 相似文献
12.
FA Bruna EG De Di Nasso M Soaje RP Deis RW Carón 《Reproduction in domestic animals》2010,45(5):796-802
The role of opioid peptides in the secretion of oxytocin (OT) and prolactin (PRL) induced by sucking was studied in goats. Seven goats were isolated with their kids (four singletons and three twins) in individual corrals 3–4 weeks after parturition. On day 1 of the experiment, the kids were separated from the does for 7 h and were weighed before and 15 min after being reunited with their mothers to assess the amount of milk obtained by sucking. The does were blood‐sampled 10 min before and at the end of the sucking period. On day 2, a similar protocol was followed, but naloxone was given immediately after the first blood sample. On day 3, the protocol was repeated but saline vehicle was injected instead of naloxone. On day 5, the naloxone experiment was repeated as on day 2. Milk ejection was evaluated as the difference in the weight of the kids before and after sucking for 15 min, and the maternal serum levels of OT and PRL were measured by radioimmunoassay. A significant decrease in the weight gain of the kids was obtained when the mothers were treated with naloxone on day 2. Consistently, serum levels of OT and PRL induced by sucking were significantly reduced; indicating that sucking‐induced OT secretion for milk ejection in lactating goats is facilitated by opioid peptides. In a second experiment performed in the same animals 10 days later, the administration of OT, immediately after naloxone administration, prevented the decrease in the weight gain induced by naloxone, suggesting that the effect of the opioid antagonist on milk ejection in goats is a result of a reduced OT secretion. The results of this study confirm the importance of sucking‐induced OT secretion for milk ejection in lactating goats, and indicate that OT and PRL secretion are regulated by opioid peptides in this species. 相似文献
13.
M. S. Awawdeh A. Q. Talafha 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(3-4):176-182
AbstractThis study investigated the effects of vitamin E and selenium (Se) injections on concentrations of vitamin E, vitamin A, and Se in blood and milk to predict when injections may improve ewes’ performance. Awassi ewes received 0 (control, 13 ewes) or 15 plus 0.05?mg/kg BW of vitamin E and Se (injected, 15 ewes) at 1 and 4 weeks postpartum. Blood and milk samples were collected weekly for 6 weeks. Injections increased vitamin E, decreased Se, and had no effects on vitamin A levels in blood. Blood vitamin E was correlated positively with vitamin A. Injections increased vitamin E, but had no effects on vitamin A or Se levels in milk. Milk vitamin A was positively correlated with vitamin E and Se levels in the control group. Milk vitamins E and A were positively correlated with their corresponding levels in blood. Milk somatic cell count was negatively correlated with blood vitamin A levels. 相似文献
14.
The activity of glutathione peroxidase (GSH-Px, E.G. 1.11.1.9.) was determined in heparinized whole blood, blood plasma and washed erythrocytes from goats before and up to 4 weeks after the administration of selenium (0.4 mg/10 kg BW) and vitamin E (20 mg/10 kg BW) or only vit. E (20 mg/10 kg BW). It was found that Se administration caused a significant increase in enzyme activity in whole blood and washed erythrocytes first detected 2 weeks after the intramuscular injection of Se. No changes were observed in plasma from the treated animals. Minor and insignificant changes were seen in the vit. E treated control animals. It is concluded that GSH-Px activity in blood plasma or serum is of no value as a short-term indicator of the selenium status of goats but whole blood is a good indicator of the long-term status. 相似文献
15.
Petr Slavik Josef Illek Michal Brix Jaroslava Hlavicova Radko Rajmon Frantisek Jilek 《Acta veterinaria Scandinavica》2008,50(1):43
Background
Selenium (Se) is important for the postnatal development of the calf. In the first weeks of life, milk is the only source of Se for the calf and insufficient level of Se in the milk may lead to Se deficiency. Maternal Se supplementation is used to prevent this.We investigated the effect of dietary Se-enriched yeast (SY) or sodium selenite (SS) supplements on selected blood parameters and on Se concentrations in the blood, colostrum, and milk of Se-deficient Charolais cows.Methods
Cows in late pregnancy received a mineral premix with Se (SS or SY, 50 mg Se per kg premix) or without Se (control – C). Supplementation was initiated 6 weeks before expected calving. Blood and colostrum samples were taken from the cows that had just calved (Colostral period). Additional samples were taken around 2 weeks (milk) and 5 weeks (milk and blood) after calving corresponding to Se supplementation for 6 and 12 weeks, respectively (Lactation period) for Se, biochemical and haematological analyses.Results
Colostral period. Se concentrations in whole blood and colostrum on day 1 post partum and in colostrum on day 3 post partum were 93.0, 72.9, and 47.5 μg/L in the SY group; 68.0, 56.0 and 18.8 μg/L in the SS group; and 35.1, 27.3 and 10.5 μg/L in the C group, respectively. Differences among all the groups were significant (P < 0.01) at each sampling, just as the colostrum Se content decreases were from day 1 to day 3 in each group. The relatively smallest decrease in colostrum Se concentration was found in the SY group (P < 0.01).Lactation period. The mean Se concentrations in milk in weeks 6 and 12 of supplementation were 20.4 and 19.6 μg/L in the SY group, 8.3 and 11.9 μg/L in the SS group, and 6.9 and 6.6 μg/L in the C group, respectively. The values only differed significantly in the SS group (P < 0.05). The Se concentrations in the blood were similar to those of cows examined on the day of calving. The levels of glutathione peroxidase (GSH-Px) activity were 364.70, 283.82 and 187.46 μkat/L in the SY, SS, and C groups, respectively. This was the only significantly variable biochemical and haematological parameter.Conclusion
Se-enriched yeast was much more effective than sodium selenite in increasing the concentration of Se in the blood, colostrum and milk, as well as the GSH-Px activity. 相似文献16.
Liesegang A Staub T Wichert B Wanner M Kreuzer M 《Journal of animal physiology and animal nutrition》2008,92(3):292-302
Vitamin E (VitE) and selenium (Se) are an essential part of the antioxidative functions of metabolism. There are situations of low supply of both micronutrients. As VitE is involved in ruminal biohydrogenation of polyunsaturated fatty acids (PUFA) and their protection against oxidation in metabolism, diets supplemented with PUFA may challenge VitE to an extent making recommended supplies insufficient. Twelve goats and sheep each were fed a diet supplemented with PUFA and characterised by low Se and limited VitE contents during the last 2 months of gestation and the first 2 months of lactation. The basal diet consisted of hay and concentrate. Six goats and sheep received extra VitE, while the control groups received no extra VitE. Blood and milk samples were taken. In addition, liver, heart muscle and spleen samples were obtained from the offspring after slaughtering at an age of 8 weeks. No significant changes were observed in serum Se and VitE. A significant increase in serum VitE concentrations between 2 and 4 weeks postpartum (pp) was evident in the supplemented kids. In 4, 6 and 8 weeks pp, the serum concentrations of VitE in the supplemented kids were significantly higher compared to the unsupplemented group. In the kids, VitE was higher in liver of the supplemented groups. There were no significant differences in response to extra VitE between sheep and goat. The kids responded to serum VitE different from that of lambs, as a significant difference was observed between supplemented and unsupplemented animals in the goat kids, but not the lambs. In conclusion, goats and sheep have to be viewed differently and may not be considered alike relating to VitE/Se metabolism and requirements, especially in young animals. 相似文献
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In 30 female goats hyperimmunized with bovine insulin, antibody concentrations in serum were determined by radioimmunoassay. Blood samples were taken from the newborn lambs immediately after birth and during the 12 weeks thereafter. Twin lambs were separated and reared in either a colostrum-fed or a colostrum-deprived group. Bovine insulin was added to the milk rations of colostrum-deprived lambs. At the age of 10 weeks, these kids were given a single dose of 1 IU of insulin in combination with complete Freund's adjuvant. Colostrum from nanny goats was also secured for further investigations. Most of the hyperimmunized adult goats responded with the development of insulin antibodies. Their kids were born without these specific immunoglobulins, but several hours after they were allowed to suckle colostrum, the antibody concentration reached values comparable with the values measured in the nanny's blood. Colostrum-deprived kids did not show an increase in binding capacity. The addition of insulin to the milk rations of bottle-fed kids did not act as a primer in antibody production. Immunoglobulins in blood serum of nanny goats and kids and in colostral whey belong mainly to the IgG class. These results indicate that in goats, maternal insulin antibodies are transferred from nanny to offspring only by colostrum. 相似文献
19.
Grace ND Ankenbauer-Perkins KL Alexander AM Marchant RM 《New Zealand veterinary journal》2001,49(1):24-28
AIM: To determine the relationships between blood selenium (Se) concentrations or glutathione peroxidase activity (GSH-Px), and milk Se concentrations in dairy cows. METHODS: Seventy-two Friesian dairy cows were either untreated or injected with 0.5, 1.0 or 2.0 mg Se/kg liveweight as barium selenate (BaSeO4) formulations, resulting in 6 groups of animals with mean blood Se concentrations that varied from 212 to 2272 nmol/l. Milk samples were collected on Days 104 and 188, and blood samples were collected prior to treatment and on Days 41, 76, 104, 188, 244, and 292 after Se injection. RESULTS: Significant quadratic relationships between blood Se and milk Se concentrations, as well as blood GSH-Px activity and milk Se concentrations, were evident at Days 104 and 188. Using combined data, these were represented by the equations: milk Se = 27.3 + 0.073 blood Se -0.00001 (blood Se)2; R2=0.79, p<0.005, and; milk Se = 34.8 + 4.99 GSH-Px -0.068 (GSHPx)2; R2=0.79, p<0.005. CONCLUSIONS: The Se status of dairy cows can be assessed from milk Se concentrations. CLINICAL SIGNIFICANCE: Bulk-tank milk Se concentrations could be evaluated as a method to assess the Se status of dairy herds. 相似文献