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1.
Byeong Joo Seo Mi Ran Mun Rejish Kumar V. J Chul-Joong Kim Insun Lee Young-Hyo Chang Yong-Ha Park 《Veterinary research communications》2010,34(4):323-333
Lactic acid producing bacterial strain Probio-16 was isolated from the swine excrements under anaerobic conditions and characterized
by morphology and biochemical characteristics. The strain was further identified by 16S rRNA gene sequencing and phylogeneitc
analysis. The antimicrobial activity of the strain was assayed by testing for growth inhibition of thirteen pathogenic microorganisms.
The strain was tested for antiviral activity against porcine rotavirus in vitro in African green monkey epithelial cell line
TF-104. Antibiotic susceptibility of the strain against 13 antibiotics was tested using disk diffusion method. Phenotypically
and through 16S rRNA gene sequences, Probio-16 was identified and named as Lactobacillus reuteri Probio-16. This strain was resistant to pH 2.0, 5% porcine bile and exhibited antimicrobial activity against all the thirteen
enteric bacterial pathogens tested. Probio-16 supernatant inhibited porcine rotavirus in vitro in TF-104 cell lines. Except
for erythromycin and penicillin G at a concentration of 4 μg/ml, Probio-16 showed resistance to all other thirteen antibiotics
tested. This study indicates L. reuteri Probio-16 as a novel strain with its tolerance to low pH and bile, antimicrobial activity, antibiotic resistance and antiviral
activity against rotavirus, and an ideal probiotic candidate for animal and human application after the proper in vivo experiments. 相似文献
2.
E Honda H Takahashi K Okazaki T Minetoma T Kumagai 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):217-224
Plaque formation, replication and related cytopathic function of 9 strains of transmissible gastroenteritis (TGE) virus were examined in primary cells and cell lines such as CPK, IB-RS-2, ESK, and PK-15 originated from porcine kidney and the effects of trypsin on the replication of TGE virus were examined in CPK cells. All strains produced a cytopathic effect and grew well in CPK cells as well as in primary porcine kidney cells. The effect of trypsin on the plaque formation was different from strains. The number of plaques produced by strains TO-163, Ukiha and Niigata increased from 2.6 to 3.52 times when trypsin was present in the medium during incubation at 37 degrees C for 1 hr after adsorption of the virus at 4 degrees C for 2 hr. The plaque sizes of TO-163, h-5, Ukiha and Niigata became larger from 1.4 to 1.7 times, when trypsin was present in the agar MEM overlay. 相似文献
3.
猪传染性胃肠炎是由猪传染性胃肠炎病毒引起的一种急性、高度接触性肠道传染病,不同年龄和品种的猪均可感染,严重危害着全世界养猪业的健康发展.疫苗接种是防控本病的关键措施之一.常规疫苗在防控猪传染性胃肠炎中发挥了巨大的作用,但在某种程度上存在不可避免的缺陷,对根除和消灭传染病带来了严重困难.随着分子生物学技术的不断发展,基因... 相似文献
4.
About twenty years ago, a new coronavirus, porcine respiratory coronavirus (PRCoV), was detected in swine herds. This virus is related to transmissible gastroenteritis virus (TGEV); however, it is not enteropathogenic but causes only minor respiratory symptoms. As PRCoV shares some epitopes for neutralizing antibodies with TGEV, it acts like a nature-made vaccine against TGEV resulting in a drastic reduction of TGE outbreaks in Europe. A major difference between the two porcine coronaviruses is a large deletion in the surface protein S gene of PRCoV. Because of this structural difference, TGEV but not PRCoV has a sialic acid binding activity that allows the attachment to mucins and mucin-type glycoproteins. The sialic acid binding activity may allow TGEV to overcome the mucus barrier in the gut and to get access to the intestinal epithelium for initiation of infection. 相似文献
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根据猪传染性胃肠炎病毒(TGEV)纤突(S)蛋白的全基因序列及表达载体质粒的基因融合特点,设计一对引物,进行PCR,获得含有TGEV S基因4个主要抗原位点的约2000 bp目的片段,将其分别与表达载体质粒pPG611.1和pPG612.1进行连接,通过电转化进入宿主菌Lacto-bacillus casei393细胞内,通过质粒提取、PCR鉴定、酶切鉴定和序列测定分析,表明TGEV S基因已成功插入到表达载体质粒中,获得了TGEV S蛋白干酪乳杆菌表达载体系统。 相似文献
7.
Cross-protection studies between the feline infectious peritonitis (FIP) and the porcine transmissible gastroenteritis (TGE) viruses were conducted in cats, pigs and pregnant gilts. Cats vaccinated with TGE virus developed neutralizing antibodies against TGE virus and low titer antibody against FIP virus detected by an indirect fluorescent antibody technique but were not protected against a virulent FIP virus challenge. Baby pigs and pregnant gilts vaccinated with FIP virus did not develop detectable antibodies to TGE virus. Nevertheless, it appeared that vaccination of swine with FIP virus conferred some immunity against TGE virus infection. Seventeen-day-old pigs vaccinated with two doses of FIP virus had a 67% survival rate following a virulent TGE virus challenge, and 75% of the 3-day-old pigs suckling either FIP or TGE-virus-vaccinated gilts survived virulent TGE virus infection in contrast to 0% survival of baby pigs suckling unvaccinated gilts. 相似文献
8.
Selection and characterization of broad‐spectrum antibacterial substance‐producing Lactobacillus curvatus PA40 as a potential probiotic for feed additives 
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下载免费PDF全文 Sung Wook Hong Jong‐Hui Kim Hyo Ju Bae Jun‐Sang Ham Jae Gyu Yoo Kun Sub Chung Mi‐Hwa Oh 《Animal Science Journal》2018,89(10):1459-1467
Lactic acid bacteria were screened for potential probiotics for use as feed additives. We obtained 3,000 isolates from feces of: cattle, dogs, goats, and infants; milk; yogurt; cheese; fermented sausages; Kimchi; and Cheonggukjang and tested their antibacterial activity toward indicator pathogens, including Bacillus cereus, Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella enterica Enteritidis. We further tested their tolerance to artificial gastric juice (1% [w/v] pepsin, pH 2.5) and bile acid (0.1% [w/v] oxgall, pH 6.8). Six isolates exhibited strong antibacterial activity against indicator pathogens. The PA40 isolate from Kimchi exhibited marked resistance to artificial gastric juice and bile acid. The antibacterial substances produced by PA40 were stable to heat, pH, and enzymes. Strain PA40 was identified as a Lactobacillus curvatus strain using chemical tests and 16S rDNA sequencing and produced 248.4 mmol/L lactic acid after 48 hr of fermentative growth. The L. curvatus PA40 strain was also highly tolerant of the artificial gastrointestinal model system. Our results indicate that L. curvatus PA40 could be used as a potential probiotic feed additive. 相似文献
9.
Enzymatic and acidic sensitivity profiles of selected virulent and attenuated transmissible gastroenteritis viruses of swine 总被引：1，自引：0，他引：1
K S Chen 《American journal of veterinary research》1985,46(3):632-636
This study identifies the in vitro differences (markers) between virulent and attenuated transmissible gastroenteritis (TGE) viruses. Exposure of virulent Miller strain and attenuated Purdue strain TGE viruses to a spectrum of acidities indicated that the Miller strain was more stable at pH 2. Acidities at or above pH 3 did not reduce viral infectivity of either strain. When virulent and attenuated viruses were exposed to gastric fluids of either fed or fasted swine, there was a similar degree of sensitivity. Carboxypeptidase B, alpha-amylase, and alkaline phosphatase present in porcine small intestinal fluids did not cause a significant difference in sensitivity between virulent and attenuated virus isolates. The digestive enzymes: trypsin, alpha-chymotrypsin, pancreatin, peptidase, and carboxypeptidase A did not (or only slightly) inactivate virulent Miller strain TGE virus, but greatly reduced infectivity of attenuated viruses (Purdue strain and TGE vaccine virus isolates). The attenuated strains were significantly more sensitive to small intestinal fluids from both fasted and fed adult swine. Differential sensitivities between virulent and attenuated TGE viruses to digestive fluids from stomach and small intestine further substantiate the notion of differential susceptibility to small intestinal proteases as a correlate of viral virulence. 相似文献
10.
Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis 总被引：21，自引：0，他引：21
A porcine respiratory, non-enteric virus which is related to the coronavirus transmissible gastroenteritis virus (TGEV) has been isolated in pigs and in cell culture. The isolate was designated TLM 83. It has become very widespread and enzootic among the swine population in Belgium and in other swine raising countries. It causes an infection of the lungs and appears to spread by aerogenic route. It does not replicate in the enteric tract. The experimental infection in conventional and gnotobiotic pigs in isolation remains subclinical. The infection, either experimental or in the field, results in the formation of antibodies which neutralise the classical enteric TGEV. Based on this relationship, this virus is assumed to be a new TGEV-related porcine respiratory coronavirus or TGEV itself which has totally lost its tropism for the enteric tract. 相似文献
11.
A Morilla A E Ritchie P Sprino M Ristic 《American journal of veterinary research》1977,38(10):1491-1495
Intestinal and cell culture-adapted viral populations of transmissible gastroenteritis (TGE) of swine were compared by means of sucrose gradient centrifugation, immunnofluorescence, electron microscopy, immune electron microscopy, statistical analysis of the number of plaque-forming units, and ultraviolet sensitivity. Results indicated that the size range and general coronavirus morphologic characteristics were shared by both viral populations. Marked morphologic variations existed among particles from both populations. Unlike the cell culture-adapted virus, the Illinois virus of intestinal origin was infractions representing 2 bands of infectivity which were isolated by the sucrose gradient centrifugation method. The intestinal and cell culture-adapted TGE viruses were similar in antigenicity and in sensitivity to ultraviolet irradiation. There was no indication of a 2nd virus in addition to the coronavirus described as the cause of TGE. 相似文献
12.
A plasmid, pG3BS, containing a cDNA clone from the 5' coding region of the peplomer glycoprotein gene appears to be specific for enteric transmissible gastroenteritis virus (TGEV) strains and for live-attenuated TGEV vaccines. This cDNA probe is used to differentiate porcine respiratory coronavirus (PRCV) isolates from TGEV field and vaccine strains by a slot blot hybridization assay. Probe pG3BS also hybridizes to canine coronavirus (CCV) RNA but does not hybridize to antigenically related feline infectious peritonitis virus (FIPV) RNA. The RNAs of 13 enteric TGEV isolates from the United States, Japan, and England, 4 US-licensed live-attenuated TGEV vaccines, and antigenically closely related CCV were detected by pG3BS. The RNAs of FIPV and 3 US isolates of PRCV did not react with pG3BS but were detected by a TGEV-derived plasmid, pRP3. Pigs infected with either PRCV or TGEV test serologically positive for TGEV antibody by the serum neutralization test. Characterization of the virus circulating in a swine herd by the pG3BS probe will differentiate between an enteric TGEV and a respiratory PRCV infection. 相似文献
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K W Theil L J Saif E H Bohl A G Agnes E M Kohler 《American journal of veterinary research》1979,40(5):719-721
A 3-day-old suckling pig with diarrhea was necropsied, and immunofluorescent microscopic examination of the small intestinal mucosa, together with immune electron microscopic examination of the large intestinal contents, provided a presumptive diagnosis of a concurrent infection with transmissible gastroenteritis (TGE) virus and porcine rotavirus. Immunofluorescent microscopic, immune electron microscopic, and serologic data obtained from gnotobiotic pigs experimentally inoculated with the large intestinal contents of the suckling pig confirmed this diagnosis. Two gnotobiotic pigs, convalescent from previous TGE viral infections, became infected with porcine rotavirus only. However, another gnotobiotic pig, convalescent from a previous porcine rotaviral infection, became infected with TGE virus only, following inoculation with the large intestinal contents of the suckling pig. 相似文献
15.
Antigenic variation of porcine transmissible gastroenteritis virus detected by monoclonal antibodies
T Hohdatsu Y Eiguchi M Tsuchimoto S Ide H Yamagishi M Matumoto 《Veterinary microbiology》1987,14(2):115-124
Mouse myeloma cells (SP2/O) were fused with spleen cells from BALB/c mice immunized with detergent-solubilized antigen of purified virus, and 21 monoclonal (MC) antibodies reactive in enzyme-linked immunosorbent assay with the TO-163 strain of porcine transmissible gastroenteritis (TGE) virus were obtained. Of these MC antibodies, 14, 6 and 1 were IgG1, IgG2a and IgM, respectively. All of the MC antibodies contained light chains of the kappa type. Of these MC antibodies, 8 were found to have neutralization (NT) activity against the TO-163 strain. Comparison of 7 strains of TGE virus by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences. These results suggest that there may be at least 6 different epitopes involved in NT reaction on the virion of the TO-163 strain. This notion was confirmed by the competitive binding assay. 相似文献
16.
Solid phase immune electron microscopy for diagnosis of transmissible gastroenteritis in pigs 总被引：3，自引：0，他引：3
A serological trapping technique is described for detecting transmissible gastroenteritis (TGE) virus in faeces. The technique involves the coating of electron microscope grids with protein A and specific TGE virus antiserum. Optimal conditions for performing this solid phase immune electron microscopy technique were a concentration 250 micrograms ml-1 of protein A; 1:100 diluted rabbit anti-TGE virus hyperimmune serum for coating the grids and overnight incubation with virus samples. The possibility of detecting coronavirus in crude faeces was highly improved with solid phase immune electron microscopy, compared with conventional negative staining electron microscopy, by specific trapping of virus and prevention of adsorption of contaminants. The sensitivity of the method was evaluated by coded investigation of a dilution series of stock virus mixed with different pools of faeces. The improvement of virus detection in faeces by solid phase immune electron microscopy, compared with standard electron microscopy was at least 100-fold. Faecal shedding of coronavirus by pigs infected with virulent and attenuated strains of TGE virus was studied. Virus detection in faeces by a standard electron microscopy technique was not practical, since the virus was obscured by a large quantity of debris in the faeces. By using solid phase immune electron microscopy, however, the aspect of the specimens on the grids improved so much that, in addition to more common immunofluorescence, the technique might be useful as a diagnostic test for TGE. Virus was detected in daily faecal samples from one or two days after experimental infection with virulent TGE virus, until death in five out of eight animals. Unlike immunofluorescence, solid phase immune electron microscopy may be used for diagnosis in living animals. 相似文献
17.
Detection of transmissible gastroenteritis coronavirus antigens by a sandwich enzyme-linked immunosorbent assay technique 总被引：4，自引：0，他引：4
S Bernard I Lantier H Laude J M Aynaud 《American journal of veterinary research》1986,47(11):2441-2444
A new sandwich enzyme-linked immunosorbent assay, using monoclonal and polyclonal antibodies, was developed to detect transmissible gastroenteritis virus antigens from cell culture and from intestinal wash or feces obtained from experimentally infected pigs. This technique was shown to be suitable for the detection of virulent field strain unadapted to cell culture. Cross reactions had not been observed with other enteric pathogens, rotavirus, porcine epizootic diarrhea virus, and Escherichia coli. 相似文献
18.
M Asagi T Ogawa T Minetoma K Sato Y Inaba 《American journal of veterinary research》1986,47(10):2161-2164
A reversed passive hemagglutination (RPHA) method was developed for the detection of transmissible gastroenteritis (TGE) virus in the fecal specimens from pigs. Ovine erythrocytes fixed with glutaraldehyde and treated with tannic acid were coated with anti-TGE virus swine antibodies, which were purified by affinity chromatographic technique linked with purified TGE virus. The RPHA test was done by the Microtiter method. Erythrocytes coated with purified specific antibodies were agglutinated by TGE virus, but not by porcine rotavirus or porcine enterovirus. The reaction was specifically inhibited by antiserum against TGE virus, confirming the specificity of the reaction. A litter of seven 3-day-old pigs was orally inoculated with TGE virus, and fecal specimens were obtained once a day and serum was obtained every 4th day. With the RPHA test, TGE virus was detected in the diarrheal feces; all of the inoculated pigs developed virus-neutralization antibody for the TGE virus. The RPHA test detected TGE virus in feces from pigs with naturally occurring diarrhea. The RPHA test detected TGE virus in 5 of 6 fecal specimens (80%), whereas the positive rate was only 50% (3/6) for the immunofluorescent staining of primary cultures of porcine kidney cells inoculated with the specimens. The advantages of the RPHA method are simplicity, high sensitivity, and rapid to do. 相似文献
19.
试验旨在研究预防犊牛腹泻复合微生态制剂3株菌种的配比优化。通过研究嗜酸乳杆菌、布氏酵母菌、枯草芽孢杆菌不同浓度人工胃液、人工肠液、牛胆盐的耐受性及其对大肠杆菌、沙门氏菌、金黄色葡萄球菌的抑菌性,确定其生物学特性;以大肠杆菌K99为指示菌,研究3种益生菌不同配比对大肠杆菌K99的抑菌效果,以确定其最佳组合。结果表明:(1)3种益生菌在不同pH人工胃培养条件下都具有较好的耐受性,其中嗜酸乳杆菌、芽孢杆菌在pH为5时,存活率达到了118.75%、142.24%,布氏酵母菌对不同pH均表现成活率达到了100%以上。(2)3种益生菌在人工胃液中9 h存活率较高,与0 h的OD600相比,布氏酵母菌、枯草芽孢杆菌、嗜酸乳杆菌存活率分别为108.7%、129.73%、96.37%。(3)在含有不同浓度的牛胆盐培养基中,布氏酵母菌、嗜酸乳杆菌、枯草芽孢杆菌存活率较对照组的存活率最高为60.49%、85.1%、47.42%。(4)3株益生菌按照不同配比在培养12 h后,试验组第2、4、8组较对照组相比差异显著(P<0.05);培养24 h后,试验组第3、4、5、6较对照组差异显著(P<0.05),组间差异不显著(P>0.05);第8个处理组在培养12 h后对大肠杆菌K99的抑菌效果较好。综合分析,3株益生菌对人工胃液、人工肠液、牛胆盐具有较好的耐受性,并具对大肠杆菌K99、沙门氏菌、金黄色葡萄球菌等致病菌均有一定的抑菌特性。嗜酸乳杆菌、枯草芽孢杆菌、布氏酵母菌最佳的优化配比为3:3:1。 相似文献
20.
In recent years, the use of in-feed antibiotics for growth and disease prevention in livestock production has been under severe scrutiny. The use and misuse of in-feed antibiotics has led to problems with drug residues in animal products and increased bacterial resistance. Chicken egg yolk antibodies (IgY) have attracted considerable attention as an alternative to antibiotics to maintain swine health and performance. Oral administration of IgY possesses many advantages over mammalian IgG such as cost-effectiveness, convenience and high yield. This review presents an overview of the potential to use IgY immunotherapy for the prevention and treatment of swine diarrhea diseases and speculates on the future of IgY technology. Included are a review of the potential applications of IgY in the control of enteric infections of either bacterial or viral origin such as enterotoxigenic Escherichia coli, Salmonella spp., rotavirus, porcine transmissible gastroenteritis virus, and porcine epidemic diarrhea virus. Some potential obstacles to the adoption of IgY technology are also discussed. 相似文献