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以密枝枸杞为试材,以"宁杞5号"枸杞为对照,采用分光光度法、Folin-Ciocalteu法、苯酚-硫酸比色法、四苯硼钠比色法、凯氏定氮法和2,6-二氯酚靛酚滴定法分别测定枸杞叶片中总黄酮、总多酚、粗多糖、蛋白质、抗坏血酸和钾元素含量,研究了密枝枸杞叶片营养品质价值。结果表明:密枝枸杞和"宁杞5号"叶中除抗坏血酸含量无显著性差异(P0.05)外,其它各成分含量均存在显著性差异(P≤0.05)。密枝枸杞叶中总黄酮、总多酚、蛋白质含量显著高于"宁杞5号"枸杞叶(P≤0.05),而粗多糖、钾的含量极显著高于"宁杞5号"枸杞叶(P≤0.01)。与"宁杞5号"枸杞叶相比,密枝枸杞叶中总黄酮、总多酚、蛋白质、粗多糖和钾的含量分别高2.97%、5.93%、6.42%、8.31%和33.76%,但抗坏血酸含量低32.34%。 相似文献
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以枸杞品种"宁杞1号"和"宁杞7号",以及枸杞品系'1401'和'1402'为试材,采用生长指标调查和光合特性测定等方法,研究了根域限制栽培模式下4个宁夏枸杞品种(系)的形态生长和光合指数差异,以探索根域限制栽培条件对不同枸杞品种(系)的形态生长和光合特性的影响.结果表明:'1401'和'1402'相对于其它2个品种生长指标较好,净光合速率(Pn)、胞间CO2浓度(Ci)、蒸腾速率(Tr)、气孔导度(Gs)等光合生理参数也均高于"宁杞7号"和"宁杞1号",但'1401'的相对叶绿素含量(SPAD)和水分利用效率(WUE)相对最低.综合分析认为枸杞优良品系'1402'更适合根域限制栽培条件. 相似文献
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宁夏中宁县、新疆精河县和青海诺木洪农场是枸杞种植的典型生态区。以不同产区"宁杞1号"和"宁杞7号"2个宁夏枸杞品种的果实形态特征为研究对象,结合发育期气象因子指标,通过分析宁夏枸杞果实形态特征与气象因子的相关性,确定影响枸杞果实形态特征的关键气象因子。结果表明:宁夏中宁县和青海诺木洪2个产区的宁夏枸杞果实纵横径的发育可以分为3个明显阶段,而新疆精河县纵径发育只有2个明显阶段,枸杞果实发育期时长由长到短依次为青海诺木洪>宁夏中宁县>新疆精河县;同时,除新疆精河县外,其它2个产区的"宁杞1号"枸杞果实的发育期都明显长于相同产区"宁杞7号"枸杞果实的发育期,但3个产区"宁杞1号"枸杞果实纵横径的平均日增长量均小于相同产区"宁杞7号"枸杞果实纵横径的平均日增长量。3个产区气象因子的动态变化趋势有所不同,相关分析及回归分析表明,日最高气温、日最低气温和平均土壤温度是影响宁夏枸杞果实形态特征的关键气象因子。 相似文献
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以"顺宝"有机肥和"甘油杂1号"甘蓝型油菜为材料,采用田间随机排列方法,并配施有机肥(T1处理)、翻压油菜(T2处理),以单施化肥为对照(CK),研究了种植绿肥对"宁杞5号"枸杞果实产量及品质的影响。结果表明:与CK相比,T1处理枸杞干果年产量显著增加24.1%,夏果和秋果百粒质量分别显著增加4.2%和4.6%;与CK相比,T2处理枸杞干果年产量显著增加19.1%,秋果百粒质量显著增加11.1%。从秋果来看,T1和T2处理干果枸杞多糖、蛋白质、黄酮含量较CK显著增加25.1%、26.4%、16.4%和34.9%、48.5%、24.8%。表明在化肥施用量减半条件下,T1和T2处理均可以提高枸杞果实产量和品质,产量的增加主要通过增加果实大小、百粒质量以及内部干物质的积累来实现。因此,枸杞行间套种油菜作为绿肥,不仅可以减少化肥施用量,还可以提高枸杞产量和品质。 相似文献
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不同产地宁夏枸杞特征化学成分及营养成分比较 总被引：2，自引：0，他引：2
以产自银川芦花台园艺场和中宁县的宁夏枸杞宁杞1号入药果实和叶为评价器官,比较了其特征
化学成分和营养成分。结果表明,不同产地的枸杞果实中天门冬氨酸、谷氨酸、丙氨酸、精氨酸和脯氨
酸含量较高,枸杞叶中天冬氨酸、谷氨酸、亮氨酸和赖氨酸含量较高。两产地的枸杞果实中各种氨基酸
含量各有高低,但差异都不明显;枸杞叶中一些氨基酸含量要高于枸杞果实,如苏氨酸、甘氨酸、缬氨
酸、异亮氨酸、亮氨酸、酪氨酸、苯丙氨酸、赖氨酸和色氨酸。甜菜碱含量中宁县枸杞风干果比芦花台
枸杞风干果高44.2 %;多糖含量芦花台枸杞风干果和风干叶均略高于中宁县枸杞风干果和风干叶;VC含
量芦花台枸杞风干果和风干叶均明显高于中宁县枸杞风干果和风干叶。不同干燥方式下,枸杞冻干果胡
萝卜素损失较大,风干果损失较小;而VC含量则是冻干果损失较小,风干果损失较大。 相似文献
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The effects of combinations of salinity (no salt, 2000 p.p.m. or 4000 p.p.m. of CaCl2 and NaCl, 1:1) and water table (30 cm, 60 cm or 90 cm from the soil surface), on the vegetative growth and tolerance of ‘Golden Japanese’ plum and ‘Mit Ghamre’ and ‘Balady’ peaches were studied. The plants were grown in lysimeters. The growth of the trunk, total shoot length, the increase in shoot length per cm and the fresh weight of top, root and total plant were reduced with increasing salinity of the irrigation water. The effect was accentuated when the plants were maintained at high water table level. The salinity treatments resulted in the death of 43%, 73% and 76% of the plants in the plum, and the ‘Mit Ghamre’ and ‘Balady’ peaches, respectively, indicating that the plum is more tolerant to salinity than the peach. The plants of the salinity treatments showed various symptoms of salt injury, such as leaf burn, defoliation, shoot die-back and finally death. In the peaches, salt injury started to occur in the first growing-season, whereas the salt injury appeared in the plum in the second growing-season. The symptoms were more pronounced in the 4000 p.p.m. treatment than in the 2000 p.p.m. treatment and were more pronounced at the high water table level. The salinity level was the predominant factor and the effect of the water table on the vegetative growth diminished with increase in the salinity level of the irrigation water. 相似文献
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AIM：To establish a fast, accurate and economical technique for culturing mouse pulmonary arteriolar smooth muscle cells (PASMCs), and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs. METHODS：In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask. Centrifugal procedure was not used during the cell passage. The cell morphology was observed under an inverted phase-contrast microscope. α-Smooth muscle actin was identified by immunocytochemistry and immunofluorescence. The effects of hypoxia on the proliferation and apoptosis of the PASMCs were detected by CCK-8 assay and TUNEL assay. RESULTS：PASMCs were identified by the methods of immunocytochemistry, immunofluorescence staining and observation of morphology. Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern. The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells. CCK-8 assay demonstrated that the A values of PASMCs exposed to hypoxia increased after 24 h (P＜0.05) as compared with normoxia. TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05). CONCLUSION：This technique for obtaining cultured mouse PASMCs is simple, fast, accurate and economical. The digestion time is easy to control. Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs. 相似文献
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小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 总被引：2，自引：0，他引：2
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。 相似文献
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钾肥在小白菜和萝卜上的施用效果 总被引：6，自引：0，他引：6
采用盆栽试验方法研究了施用钾肥对小白菜、萝卜生长发育、产量、品质及钾素含量的影响。结果表明 ,施用钾肥对小白菜和水萝卜的株高、叶片数无显著的影响 ,但可提高水萝卜块根的产量 ,提高幅度为 31.0 4 %～ 97.93%。施用钾肥可提高小白菜和萝卜的蛋白质和VC含量 ,降低硝酸盐含量 ,增加植株的钾素含量 相似文献
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Fresh onion seeds desiccated to 6.0% seed moisture content (SMC) were stored in various packaging materials under different storage conditions. Seeds packed in aluminum-laminated pouches beside those stored with silica gel at 25 °C maintained satisfactory germinability and vigour after 12 months. Desiccated seeds stored in moisture impervious containers produced more vigourous seedlings. Germination potential of onion seeds increased with reduced SMC besides storage in moisture impervious packets along with desiccants as physiological and biochemical attributes are regulated. Seed viability and vigour decreased with accelerated ageing due to increased lipid peroxidation, decreased activities of several free radical and peroxide scavenging enzymes. Electrical conductance of seed leachates also increased with ageing. Thus, adoption of appropriate storage temperature and moisture control techniques would significantly affect onion seed quality, which was due to minimum accumulation of free peroxide radicals and enhanced activity of free radical scavenging enzymes. 相似文献