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本试验采用水提醇沉法提取玉米须多糖,在不同的煎煮时间、提取温度、水料比条件下,探索最佳提取条件。并用不同体积的乙醇沉淀多糖,得出最佳醇沉体积比。水提后的玉米须残渣用β-葡聚糖酶和木聚糖酶酶解提取,比较用不同的酶量酶解条件下的最适提取条件,提取的粗多糖用三氯乙酸(TCA)法除多糖蛋白,用紫外扫描检验蛋白是否除尽。结果表明,玉米须多糖提取的最佳条件为:80min、80℃、18倍的水料比、2.5倍体积乙醇沉淀效果最佳;3%浓度的TCA溶液除蛋白质效果最好;通过紫外扫描检测纯化样品表明糖结合蛋白基本除尽。 相似文献
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本试验采用水提醇沉淀法研究了影响可溶性荷叶多糖提取的因素并测定了其多糖含量.通过控制和改变提取时间、提取温度、乙醇沉淀体积倍数研究这些因素对提取结果的影响,并且利用三氯乙酸法除蛋白质.时荷叶多糖进行初步纯化.结果表明,荷叶多糖最佳提取的条件为:80℃、3h、2.5倍体积乙醇沉淀效果最佳,粗糖提取率为1.795%,糖相对含量为7.23%,3%浓度的TCA溶液除蛋白质效果最好,糖相对含量为9.98%. 相似文献
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本试验采用水煮醇沉淀法研究了影响茶叶多糖(TPS)提取的因素,并利用苯酚-硫酸法测定多糖含量.通过控制和改变提取时间、提取温度、乙醇沉淀体积倍数研究这些因素对提取结果的影响,并比较了Sevag法与三氯乙酸(TCA)法除蛋白质的效果,再利用凝胶柱层析法时TPS进行初步纯化.结果表明.TPS最佳提取的条件为100℃、4 h、2.5倍体积乙醇沉淀效果最佳.粗糖提取率为9.1624%,相对含量为44.6%.TCA法除蛋白质效果较好,经凝胶柱层析,确定本方法所提取的茶叶多糖为一种糖蛋白. 相似文献
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为了对中药复方免疫增强剂中的多糖进行纯化,试验采用苯酚硫酸法测定多糖含量,然后以AB-8大孔吸附树脂为分离材料,以乙醇为洗脱剂,系统研究上样液浓度、上样量、上样速率、洗脱剂乙醇浓度、洗脱速率、洗脱剂用量等参数对多糖吸附-解吸效果的影响。结果表明:上样液浓度为5.93 mg/mL,上样量为1倍量树脂体积(BV),上样液速率为每小时2倍树脂体积数;洗脱剂乙醇浓度为50%,洗脱速率为每小时3倍树脂体积数,洗脱剂用量为3倍量树脂体积;吸附率为74.3%,解吸率为93.6%。说明AB-8大孔树脂可用于纯化中药复方免疫增强剂中的多糖。 相似文献
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试验采用L9(3)3正交试验设计,以蛋白去除率和多糖损失率为指标分别研究了Sevage法和三氯乙酸法除蛋白的最佳工艺参数,并对两种方法进行了比较。试验结果表明,Sevage法除沙葱多糖溶液中蛋白质的最佳工艺参数为:Sevage试剂添加量为沙葱多糖溶液体积的1/4,氯仿与正丁醇的比例为3:1,振摇时间为30 min。在此条件下,沙葱多糖的除蛋白率为43.82%,多糖损失率为4.67%;三氯乙酸法除沙葱多糖溶液中蛋白质的最佳工艺参数为:三氯乙酸浓度为15%,添加量是沙葱多糖溶液体积的1倍,即1:1添加,振摇时间为20 min。此时的除蛋白率为87.33%,多糖损失率为55.59%。 相似文献
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为了优化Sevag法脱鹿药粗多糖蛋白工艺条件,以牛血清白蛋白为标准品测定蛋白质含量,以葡萄糖为标准品测定多糖含量,以蛋白质脱除率、多糖保留率及综合评分为指标,通过正交试验考察氯仿与正丁醇的比例、多糖液与Sevag液的比例、振荡时间、洗脱次数四因素对脱蛋白效果的影响,确定鹿药多糖脱蛋白的最佳工艺条件。结果表明Sevag法脱鹿药多糖蛋白的最佳条件为氯仿∶正丁醇=4∶1,多糖溶液(2 mg/mL)∶Sevag试剂=2∶1,振荡时间15 min,洗脱6次,从综合评分来看,主要的影响因素是脱蛋白次数和振荡时间。Sevag法脱鹿药多糖蛋白的方法简便,工艺稳定且可有效提高多糖的纯度。 相似文献
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聚合草鲜草叶蛋白提取条件研究 总被引：7，自引：1，他引：6
以紫花聚合草为材料,研究固液比、盐浓度、凝絮方法对叶蛋白提取率和蛋白质提取率的影响.结果表明:各单因素最佳条件分别为:固液比为1:6、加盐浓度0.6%、乙醇浓度65%、pH值1.5、三氯乙酸沉淀法叶蛋白提取率和蛋白质提取率均较高.对固液比、盐浓度、凝絮方法3因素3水平正交试验结果表明,影响聚合草叶蛋白提取率的因素依次为凝絮方法固液比加盐量,最佳条件组合为固液比1:7、盐浓度0.3%、乙醇法沉淀. 相似文献
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产肠毒素性大肠杆菌工程菌株pSLM004在限定性培养基上培养后,上清液经硫酸铵沉淀、丙酮抽提、Sephadex G-25凝胶过滤、DEAE-Sephadex A-25离子交换层析、Bio-gel P-4凝胶及反相高压液相层析,获得的STa纯化倍数为302.7倍,有效剂量达10.72ng/鼠单位,回收率为9.9%。纯化的STa在Sephadex G-25凝胶过滤时出现1个蛋白峰,聚丙烯酰胺凝胶电泳(PAGE)呈现1条蛋白带;在100℃30min不失活,121℃15min则失活;在pH 1～10范围内保持活性;胰蛋白酶、链霉蛋白酶对其活性无影响;2-巯基乙醇、二硫苏糖醇(DTT)可使其迅速失活,表明在STa分子中有生物活性所必需的二硫键存在。 相似文献
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LU Guang-fu CHEN Xiao-lan HUANG Ya-qi JIANG Chun-mao YU Jie QU Jing-wen GENG Qian-li 《中国畜牧兽医》2016,43(7):1922-1927
The experiment was conducted to study the best extract process of mulberry leaf polysaccharide,three factors and three levels orthogonal test was designed to optimize the extraction process.Three factors were considered,including ratio of solid to liquid,decoction time,the time of extracting and the yield of mulberry leaf polysaccharide was determined with phenol-sulfurie acid colorimetry as the evaluate index.Meanwhile,the influence of ethanol concentrations on mulberry leaf polysaccharide extract was studied.The results showed that decoction times could greatest affect the extraction percent of the polysaccharides.The extraction percent of polysaccharide was the highest (4.94%) when the rate of solid to liquid was 25,the time of extracting was 2 h and decoction times was 2.While the most appropriate process was 25 times for the rate of sdid to liquid,2 h for extracting and decoction times was 3,considering with practical situations.The concentration of ethanol would significantly affect polysaccharides yield and purity.When the concentration of ethanol was 70%,the yield of mulberry leaf polysaccharid was highest.When the concentration of ethanol was 40%,the content of mulberry leaf polysaccharide was highest (41.5%),significantly higher than other ethanol concentration (P < 0.05).It suggested that the higher the ethanol concentration,the lower the polysaccharide content,but the higher the yield.The optimum extraction of mulberry leaf polysaccharides were that the rate of solid to liquid was 25,extractign for 2 h,decoction 3 times and 70% ethanol precipitation. 相似文献
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为探究桑叶粗多糖最佳提取工艺,利用正交试验设计对桑叶粗多糖的提取工艺进行优化。以桑叶粗多糖得率为评价指标,以加水倍数、煎煮时间、煎煮次数为考察因素,设计3因素3水平正交试验,采用苯酚一硫酸比色法进行桑叶粗多糖含量测定,比较得出桑叶水提物的最佳制备工艺,并在此基础上考察不同浓度乙醇对桑叶粗多糖提取的影响。结果显示,煎煮次数对桑叶粗多糖提取率影响最大,当加水倍数为25倍,煎煮时间为2 h,煎煮2次时桑叶水提物中桑叶粗多糖提取率最高,为4.94%。但结合影响因素分析,最终确定桑叶水提物的最佳提取工艺为加25倍水,煎煮2 h,煎煮3次。乙醇浓度对桑叶粗多糖的含量及得率影响很大,70%乙醇醇沉所得水提物中桑叶粗多糖质量最大,40%乙醇醇沉所得桑叶粗多糖含量最高,平均含量达41.5%,显著高于其他乙醇浓度（P < 0.05）,提示乙醇浓度越高,桑叶粗多糖含量越低,但得率越高。桑叶粗多糖最佳提取工艺为：加水倍数为25倍,煎煮时间2 h,煎煮3次,采用70%乙醇醇沉。 相似文献
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本试验旨在通过优化提取中药复方宫炎净中的有效成分生物碱,得到最佳提取工艺。试验通过探究乙醇体积分数、超声提取时间、超声提取次数及料液比这4个单因素对提取效果的影响,以盐酸水苏碱为标准品,采用L9(34)正交设计法优选中药复方宫炎净中总生物碱的提取工艺。结果显示中药复方宫炎净中总生物碱的最佳提取工艺为乙醇体积分数为70%、超声提取时间为25 min、超声提取3次、料液比为1:10,且在影响中药复方宫炎净中生物碱提取的因素中,影响大小依次为料液比、乙醇体积分数、超声提取次数、超声提取时间。 相似文献
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为优化蛹虫草多糖的纯化工艺,以蛋白去除率和多糖保留率为指标,比较Sevage法、改良的Sevage法、澄清剂法三种方法对粗多糖中蛋白杂质的去除效果。本试验依次采用DEAE－52、Sephdex G －100葡聚糖凝胶柱层析进一步分离纯化蛹虫草多糖,再经Sephdex G－200柱色谱及高效液相凝胶色谱进行纯度检验。结果显示：澄清剂法蛋白去除率为93．4％,多糖保存率为90．7％;DEAE －52柱层析得到一个洗脱峰CP ,再经Sephdex G －100柱层析后得到两个洗脱峰CP－1和CP－2。对CP－1和CP－2进行纯度检验,发现两者皆为均一性多糖。 相似文献
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M Kawamura F Ohashi F Murakami N Takai R Nishimura N Sasaki A Takeuchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(5):951-955
Sera of dogs with gentamicin-induced uremia were analyzed by high performance liquid chromatography system with strongly basic macroreticular anion exchange resin. Satisfactory separation of peaks was achieved with good reproducibility after deproteinization of sera with trichloroacetic acid at a final concentration of 3%, confirming that the system was suitable for qualitative analysis of uremic serum. The chromatograms showed that the number of peaks and the peak area had relation to concentrations of serum urea nitrogen or creatinine and severity of uremia. Four peaks were selected as suspected canine uremic peaks with high correlation to serum creatinine concentrations which were hardly influenced by extrarenal factors. The results suggested that these four fractions might contain uremic toxins. 相似文献
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In vivo temporal changes in ruminal liquid flow, liquid volume and VFA concentrations were determined in growing steers following once-daily feeding. Crossbred beef steers (n = 8; 278 +/- 8 kg), used in a crossover design, were trained to consume 180% of their maintenance energy intake within 2 h of either a high-forage (54% hay: 46% concentrate; HF) or high-concentrate (28% hay: 72% concentrate; HC) diet. For each animal on each diet, ruminal VFA concentrations, liquid volumes and liquid dilution rates were determined during a 2-h period before feeding and during three consecutive 2-h periods following feeding. Water was withheld during these periods to observe physiologically rather than behaviorally induced changes. At equal energy intakes, diet alone had no effect on VFA concentrations (P greater than .10), although changes in total VFA, acetic and isobutyric acids differed during the time periods following feeding due to diet (diet x time period interaction; P less than or equal to .10). Ruminal volume and dilution rate were altered in a reciprocal manner due to diet, with greater volumes and lower dilution rates observed in HC than in HF steers. Differences among time periods were observed for dilution rate and propionic acid concentrations. In general, these differences can be explained by comparison of the prefeeding time period with the postfeeding time periods. A numerical, but not statistically significant, increase in ruminal volume was observed following feeding. The effects of diet on volume and liquid dilution rate of the rumen must be considered when assessing total nutrients available for animal absorption. 相似文献
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复方中药芪苓制剂多糖超声提取工艺研究 总被引：1，自引：0，他引：1
研究超声波辅助法提取中药芪苓制剂多糖的工艺。通过正交试验设计L9（3^4）提取多糖,以蒽酮-硫酸法测定多糖含量,得出优化工艺的条件为：提取时间60min,提取温度80℃,料液比1：10,超声功率300W。超声波辅助提取芪苓制剂多糖,能耗低,用时短,多糖得率5．96％,是传统方法的3倍,可以作为提取芪苓制剂多糖或其它中药多糖的首选方法。 相似文献
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目的:优化高热清口服液的制备工艺。方法:采用正交设计法研究水提取的最佳条件,比较3种醇沉浓度(50%、60%、75%)对高热清口服液中黄芩苷含量的影响;采用高效液相色谱(HPLC)法测定黄芩苷的含量。结果:以10倍量水,浸泡0.5h,提取3次,每次提取1.5h时,水提液中黄芩苷含量最高;3种醇沉浓度的黄芩苷含量无显著性差异(P0.05),以50%醇沉浓度时成本最低。根据最佳工艺路线制备高热清口服液,3批成品的黄芩苷含量无显著性差异(P0.05),薄层鉴别与pH值检查均符合质量标准规定。结论:采用正交试验设计以黄芩苷的含量做为评价指标,是筛选其不同制备工艺的有效方法。按照最佳工艺路线制备的高热清口服液成品质量可控、稳定性良好。 相似文献