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1.
The amount of aflatoxin extracted from raw peanuts by using the water-slurry modification of AOAC Method II was determined for 49 different combinations of methanol concentrations and solvent/peanut ratio. Results indicate that the amount of aflatoxins B1 and B2 extracted from raw peanuts is a function of both methanol concentration and solvent/peanut ratio, and a cubic equation was developed, using regression techniques, to describe the combined effects. From the functional relationship, the predicted methanol concentration and solvent/peanut ratio that extracts the most aflatoxin B1 was computed to be 60.0% and 10.8 mL solvent/g peanuts, respectively. This combination extracted 12.1% more aflatoxin than did AOAC Method II.  相似文献   

2.
An improved enzyme-linked immunosorbent assay (ELISA) for aflatoxin B1 in cornmeal and peanut butter was developed. Aflatoxin B1 in cornmeal and peanut butter samples was extracted with 70% methanol in water containing 1% dimethylformamide diluted with assay buffer to a final concentration of 7.0% methanol, and directly subjected to an ELISA procedure that took less than 1 h for quantitative analysis and less than 30 min for screening tests. Analytical recoveries for 5-100 ppb B1 added to the cornmeal and peanut butter were 91 and 95.4%, respectively. The interwell and interassay coefficient of variation was 10% or less at the 20 ppb level and above. Agreement for B1 levels in more than 30 naturally contaminated corn, mixed feed, and peanut butter samples was excellent between the ELISA data and the data obtained from different independent laboratories using TLC or other analytical methods.  相似文献   

3.
Batch extraction of zein from dry‐milled whole corn with ethanol was optimum with 70% ethanol in water, an extraction time of 30–40 min, and temperature of 50°C. High yields (60% of the zein in corn) and high zein contents in the extracted solids (50%) were obtained at a solvent‐to‐solids ratio of 8 mL of 70% ethanol/g of corn. However, zein concentration in the extract was higher at lower ratios. Multiple extraction of the same corn with fresh ethanol resulted in a yield of 85% after four extractions, whereas multiple extractions of fresh corn with the same ethanol resulted in high (15 g/L) zein concentration in the extract. Optimum conditions for batch extraction of zein were 45°C, with 68% ethanol at a solvent‐to‐solids ratio of 7.8 mL/g for an extraction time of 55 min. Column extractions were also best at 50°C and 70% ethanol; a solvent ratio of 1 mL/g resulted in high zein concentrations in the extract (17 g/L) but yields were low (20%).  相似文献   

4.
A direct competitive enzyme-linked immunosorbent assay (ELISA) screening method for aflatoxins at 20 ng/g was studied by 12 collaborators. Test samples of peanut butter were extracted by blending with methanol-water-hexane (55 + 45 + 100) and heating the test extracts on a steam bath; test samples of the other commodities were extracted by blending with methanol-water (80 + 20). All test extracts were filtered and the filtrates were diluted with buffer to a final methanol concentration of less than 30%. Each diluted filtrate was applied to a cup containing a filter with immobilized polyclonal antibodies specific to aflatoxins B1, B2, and G1. Aflatoxin B1-peroxidase conjugate was added, the cup was washed with water, and a mixture of hydrogen peroxide and tetramethylbenzidine was added. The test sample was judged to contain greater than or equal to 20 ng aflatoxins/g when, after exactly 1 min, no color was observed on the filter; when a blue or gray color developed, the test sample was judged to contain less than 20 ng aflatoxins/g. All collaborators correctly identified naturally contaminated corn and raw peanut positive test samples. No false positives were found for controls containing less than 2 ng aflatoxins/g. The correct responses for positive test samples spiked at levels of 10, 20, and greater than or equal to 30 ng aflatoxins/g (the ratio of B1:B2:G1 was 10:1:3) were 52, 86, and 96%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

5.
A simple, rapid, and solvent-efficient method for determining aflatoxins in corn and peanut butter is described. Aflatoxins B1, B2, G1, and G2 were extracted from 50 g sample with 200 mL methanol-water (85 + 15). A portion of the extract was diluted with 10% NaCl solution to a final concentration of 50% methanol, and then defatted with hexane. The aflatoxins were partitioned into chloroform. The chloroform solution was evaporated, and the residue was placed on a 0.5 g disposable silica gel column. The column was washed with 3 mL each of hexane, ethyl ether, and methylene chloride. Aflatoxins were eluted with 6 mL chloroform-acetone (9 + 1). The solvent was removed by evaporation on a steam bath, and the aflatoxins were determined using thin layer chromatography (TLC) with silica gel plates and a chloroform-acetone (9 + 1) developing solvent. Overall average recovery of aflatoxin B1 from corn was 82%, and the limit of determination was 2 ng/g. For mass spectrometric (MS) confirmation, aflatoxin B1 in the extract from 3 g sample (20 ng/g) was purified by TLC and applied by direct on-column injection at 40 degrees C into a 6 m fused silica capillary gas chromatographic column. The column was connected directly to the ion source. After injection, the temperature was rapidly raised to 250 degrees C, and the purified extract was analyzed by negative ion chemical ionization MS.  相似文献   

6.
This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 μg/kg to 106 μg/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 μg/kg) and ochratoxin A (相似文献   

7.
腐殖酸对磷在红壤中有效性的影响   总被引：3，自引：0，他引：3  
An investigation was conducted to study the effect of humic substance （HS） on the phosphorus （P） solubility in acidic soil. The soil （2.5 g）, HS （0, 0.5, and 2.5 g）, and P as monocalcium phosphate （0.31 and 1.25 g P kg^-1 soil） were mixed with 50 mL distilled water and two different sequences of adding HS and P were used. The results indicated that the P concentration in water and 0.01 mol L-1 CaCl2 solution increased with increasing amounts of humic substance. The concentrations of Fe and Al were also increased. However, Olsen P decreased with increasing amount of humic substance. Water-soluble P concentrations from P rates at 0.31 and 1.25 g P kg^-1 soil in the treatment with 0.5 g （2.5 g） humic substance addition were 360% and 70% （500% and 90%） higher, respectively, than those in the treatment with no humic substance addition. P extracted by 0.01 mol L^-1 CaCl2 in the treatments with 0.5 and 2.5 g humic substance addition was increased by 400% and 540%, respectively, compared with that in the treatment without humic substance at the rate of 0.31 g P kg^-1 soil, while the corresponding P concentrations were increased by 80% and 90% at the rate of 1.25 g P kg^-1 soil. The order of mixing humic substance and phosphate did not significantly affect desorbed P and labile P extracted with CaCl2.  相似文献   

8.
A new extraction and chromatographic procedure to quantify free and esterified ergosterol in tomato products was devised. The extraction solution was composed of a dichloromethane/methanol mixture in a 2:1 (v/v) ratio. This extraction solvent allowed for higher ergosterol recovery from tomato products (an average of 25% more) compared to hexane, which is frequently employed for ergosterol extraction. Both free and esterified ergosterol were determined by HPLC reverse-phase chromatography employing a Nova-Pak C-18 column (300 x 3.9 mm), filled with 4 mm average particle size and a guard column of the same material. The elution was performed at a flow rate of 1 mL. min(-1) with a linear gradient of solvent A (methanol/water, 80:20, v/v) and solvent B (dichloromethane). The gradient, starting at sample injection, was from 0 to 50% B for 20 min for the free ergosterol analysis and additional 15 min at 50% B to analyze the ergosterol esters. This technique has proven to be more sensitive for ergosterol determination than other reported chromatographic procedures. Moreover, ergosterol esters, extracted from various fungal sources, separated well and were easily quantified.  相似文献   

9.
A high pressure liquid chromatographic method has been developed for determining aflatoxins B1, B2, G1, and G2 in peanut butter. The method is based on extraction with acidified aqueous methanol, partition of the aflatoxin into methylene chloride, and purification of the extract on a 2 g silica gel column. The extracted aflatoxins are resolved on a microparticulate (10 micrometer) porous silica gel column in ca 10 min with a water-washed chloroform-cyclohexane-acetonitrile solvent that contains 2% isopropanol. The fluorescence detection system determines aflatoxins B1, B2, G1, and G2 at low levels, i.e., 0.25 ppb B1, 0.5 ppb G1, and 0.2 ppb B2 and G2. Multiple assays of 5 samples of naturally contaminated peanut butters containing total aflatoxins (B1 + B2 + G1 + G2) at levels of 1, 2, 3, 9, and 17 ppb gave intralaboratory coefficients of variation of 7, 4, 4, 11, and 3%, respectively. Samples spiked at levels of 5, 9, and 17 ppb total aflatoxins showed recoveries of 79, 81, and 81%, respectively.  相似文献   

10.
Pressurized liquid extraction (PLE) was used to extract anthocyanins from the freeze-dried skin of a highly pigmented red wine grape with six solvents at 50 degrees C, 10.1 MPa, and 3 x 5 min extraction cycles. Temperature (from 20 to 140 degrees C in 20 degrees C increments) effects on anthocyanin recovery by acidified water and acidified 60% methanol were also studied. Acidified methanol extracted the highest levels of total monoglucosides and total anthocyanins, whereas the solvent mixture (40:40:20:0.1 methanol/acetone/water HCl) extracted the highest levels of total phenolics and total acylated anthocyanins. Acidified water extracts obtained by PLE at 80-100 degrees C had the highest levels of total monoglucosides, total acylated anthocyanins, total anthocyanins, total phenolics, and ORAC values. Acidified methanol extracts obtained by PLE at 60 degrees C had the highest levels of total monoglucosides and total anthocyanins, whereas extracts obtained at 120 degrees C had the highest levels of total phenolics. High-temperature PLE (80-100 degrees C) using acidified water, an environmentally friendly solvent, was as effective as acidified 60% methanol in extracting anthocyanins from grape skins.  相似文献   

11.
调亏灌溉对棉花生长、生理及产量的影响   总被引：2，自引：0，他引：2  
通过盆栽试验在棉花不同生育时期进行不同程度的调亏灌溉试验。结果表明,调亏灌溉对棉花株高、蕾铃脱落、成桃数等影响较显著;苗期和吐絮期重度水分亏缺、蕾铃期中度水分亏缺均有利于棉花产量的形成。棉花的光合速率和气孔阻力随土壤含水量变化的阈值约在14％土壤含水量水平,该阈值相当于田间持水量的60％～65％。合理的棉花调亏灌溉制度是苗期控制水分供应,土壤含水量维持在田间持水量的55％～60％;蕾期和花铃期是棉花的需水关键期,土壤含水量应分别保持在田间持水量的65％和70％左右;吐絮期应控制水分供应,土壤含水量维持在田间持水量的50％～55％。  相似文献   

12.
Peanut roots as a source of resveratrol   总被引：12，自引：0，他引：12  
A potent antioxidant, resveratrol (3,4',5-trihydroxystilbene), was extracted using 80% methanol from peanut roots (Arachis hypogaea L.), isolated with a solid-phase extraction column, purified by a semipreparative HPLC, and identified with 1H NMR and MS. The highest and lowest resveratrol contents in the peanut roots of 2000 fall and 2001 spring crops were 1.330 and 0.130 mg/g and 0.063 and 0.015 mg/g, respectively. When the dehydrated peanut root powders of spring and fall crops were combined and cooked with pork-fat patties (1%, w/w) and the separated oils were stored at 60 degrees C for conjugated diene hydroperoxide (CDHP) determination, CDHP contents of the control oils increased after 3 days of storage, whereas the contents in the peanut root-treated oils of spring and fall crops did not increase after 9 and 15 days of storage, respectively. It is of merit to find that peanut roots, usually left in the field as agricultural waste, contain resveratrol and bear potent antioxidative activity.  相似文献   

13.
水钾耦合对褐土养分及花生养分累积的影响   总被引：1，自引：1，他引：0  
为明确水钾耦合对土壤养分含量及花生养分吸收累积量的影响,以"花育25"为试验材料,采用水分(35%,50%,65%,80%的田间持水量)和钾肥(0,0.15,0.30,0.45g K_2O/kg)2因素4水平随机区组设计,通过遮雨棚盆栽试验探讨水钾耦合下褐土有机质、全量(全氮、全磷和全钾)和速效养分(碱解氮、有效磷和速效钾)含量的变化,以及花生植株养分累积量的差异。结果表明:钾肥用量增加会促进有效氮的吸收;在土壤水分缺乏时,水分胁迫低钾(W_1K_1)和轻度胁迫低钾(W_2K_1)两个处理在土壤全磷含量下降时有效磷含量不降反增,这表明施入少量钾肥有助于旱地磷的释放。施低钾K_1(135kg/hm~2)促进土壤速效钾的增加及土壤养分的平衡,较初始土壤提高0.43~0.59倍,且随钾肥用量的增加而不断升高。相同钾肥用量下,花生植株氮、磷和钾累积量随灌水量的增加均呈上升趋势;氮吸收量仅在水分胁迫时随着钾肥用量的增多而先增后减;除水分充足(W_4)外,在其他灌水处理下植株磷累积量随钾肥用量的增加均表现为先增后降;而钾累积量在各土壤水分下均随钾肥用量的增加呈现"低-高-低"的变化趋势,最高值均在中钾K_2(270kg/hm~2)处理。花生植株对营养元素的吸收累积与总生物产量和荚果产量相关性均达显著(p0.05)或极显著(p0.01)水平,总生物量与荚果产量呈极显著(p0.01)相关。综合考虑土壤养分的可持续供应、花生养分的累积和产量形成,建议土壤水分保持在65%FC,钾肥(K_2O)用量控制在135~270kg/hm~2为宜。  相似文献   

14.
An efficient and sensitive liquid chromatographic method is described for the determination of the anthelminthic drug levamisole, in muscle, liver, kidney and fat of sheep, pigs and poultry, using thiabendazole as internal standard. Samples were extracted by homogenizing with chloroform, and were applied to Supelco Si solid-phase extraction columns and eluted with methanol. Chromatographic analysis was performed on a LiChrospher 60 RP-Select B column using methanol/ammonium acetate buffer 0.05 M (55/65, v/v) as mobile phase and reading at 220 nm. The quantification limit for the assay was 4 ng/g. Mean recoveries were about 84% for liver, 85% for kidney, 89% for muscle and 84% for fat. The assay has been used for statutory testing purposes.  相似文献   

15.
Aflatoxin B1 (B1), T-2 toxin (T2), and ochratoxin A (OA) were assayed in a single extract from barley grain by using competitive enzyme linked immunosorbent assays (ELISAs) with monoclonal antibodies. B1 and T2 monoclonal antibodies were conjugated to horseradish peroxidase for direct competitive ELISA while an indirect competitive ELISA was used for OA determination. The competitive ELISA detected 0.1 ng/mL of B1, 10 ng/mL of T2, or 1 ng/mL of OA. Acetonitrile-0.5% KCl-6% H2SO4 (89 + 10 + 1) extracts of barley grain either were diluted 1:10 for direct assay or were subjected to a simple liquid-liquid cleanup procedure to concentrate the extract 10:1 before assay. For cleanup, water was added to the acetonitrile extract to partition water-soluble interfering substances, and then the mycotoxins were re-extracted with chloroform. The chloroform extract was evaporated to dryness and redissolved in Tris HCl buffer for ELISA. The mean recoveries from barley spiked with 4-60 ng/g of B1, 50-5000 ng/g of T2, and 5-500 ng/g of OA were, respectively, 93.8, 80.6, and 95.8%. The mean within-assay, inter-assay, and subsample coefficients of variation by ELISA of barley grain colonized with toxigenic fungi were less than 12% for B1 and OA but as high as 17% for T2.  相似文献   

16.
Olive mill waste is a potential source for the recovery of phytochemicals with a wide array of biological activities. Phytochemical screening of hexane, methanol, and water extracts revealed a diversity of compounds, perhaps overlooked in previous studies through intensive cleanup procedures. Methanol and water extracts contained large amounts of biophenols, and further testing of polar extraction solvents, including ethyl acetate, ethanol, propanol, acetone, acetonitrile, and water/methanol mixtures, highlighted the latter as the solvent of choice for extraction of the widest array of phenolic compounds. Stabilization of the resulting extract was best achieved by addition of 2% (w/w) sodium metabisulfite. Quantitative data are reported for nine biophenols extracted using 60% (v/v) methanol in water with 2% (w/w) sodium metabisulfite. Six compounds had recoveries of greater than 1 g/kg of freeze-dried waste: hydroxytyrosol glucoside, hydroxytyrosol, tyrosol, verbascoside, and a derivative of oleuropein.  相似文献   

17.
The Associatian of Official Analytical Chemists approved method for quantification of fumonisin B(1) (FB(1)) in corn meal or corn-based food products includes extraction into methanol (MeOH)/water (3:1, v/v). Disposal of the extraction medium can pose safety and environmental problems. To secure a rapid and inexpensive screen for FB(1) contamination, a sensitive competitive ELISA using a rabbit polyclonal antibody was developed. This assay was used in a comparative study measuring the extraction efficiency of FB(1) in aqueous or organic solvent buffers using 16 field corn samples. An aqueous phosphate buffer was found to be suitable for extracting FB(1), thus eliminating the need for organic solvents. HPLC and ELISA determinations compared well in fortified samples at known concentrations between 1 and 50 microg/mL of extract. Overestimation at levels >50 microg/mL were common. The characteristics and application of the ELISA for screening purposes are discussed.  相似文献   

18.
The present study reports a fast, economical, and feasible way to extract antioxidant phenolics from artichoke byproducts: raw artichoke (RA), blanched (thermally treated) artichoke (BA), and artichoke blanching waters (ABW). These byproducts represent a huge amount of discarded material in some industries. Two protocols, with possible industrial applicability, based on both methanol and water extractions were used. Phenolic contents (expressed as caffeic acid derivatives) (grams per 100 g of dry extract) were 15.4 and 9.9 for RA when extracted with methanol and water, respectively; 24.3 and 10.3 for BA when extracted with methanol and water, respectively; and finally, 11.3 g of phenolics/100 mL of ABW. Therefore, methanol extracts yielded more phenolics than water extracts, especially when BA byproducts were used. The higher amount of phenolics in BA could be due to the inactivation of polyphenol oxidase (PPO) at the industrial scale (due to blanching process), avoiding PPO-catalyzed oxidation of these phenolics, a phenomenon that could occur in RA byproducts. Artichoke extracts from industrial byproducts showed a high free radical scavenging activity (versus both DPPH* and ABTS*+ radicals) as well as capacity to inhibit lipid peroxidation (ferric thiocyanate method). According to these results, the use of artichoke extracts from industrial byproducts as possible ingredients to functionalize foodstuffs (to decrease lipid peroxidation and to increase health-promoting properties) is suggested.  相似文献   

19.
Compositional differences between soil solutions obtained by different methods have frequently been reported; variations in the soi1: solution ratio may explain these results. In this study we compared the amount and composition of phosphorus (P) in soil leachates and soil solutions from a temperate grassland soil in northeast Scotland and determined the influence of soi1:solution ratio on P fractions in soil water extracts. Leachates were collected from intact soil cores over 6 months, the cores were then destructively sampled, and soil solutions obtained by centrifuging. Molybdate reactive P (MRP) represented 71% of the total dissolved P (TDP) in soil leachates but only 54% in soil solutions. The MRP component in soil water extracts increased from 71% to 92% as the soi1:solution ratio increased from 1:15 to 1:15·4, while the dissolved organic P (DOP) component decreased from 26% to 6%. As the soil:solution ratio increased the amount of MRP extracted increased; by contrast the amount of DOP and dissolved condensed P (DCP) extracted remained constant. While the MRP component is regulated by soil sorption processes, the supply and amount of DOP and DCP is probably related to biological activity. Dissolved organic carbon (DOC) extracted at wide soi1:solution ratios contained a smaller proportion of P than that extracted at narrower ratios. The results indicate differences in the behaviour of P fractions in the soil at various soi1:solution ratios and that these are reflected in the P composition of soil solution and leachate.  相似文献   

20.
Abstract

Manure phosphorus (P) extraction and storage procedures were evaluated, and manure types were characterized for extractable P. The objectives of this research were to evaluate manure P extraction and sample storage procedures and to characterize manure types for water‐extractable P (WEP) and NaHCO3 P (BiEP). Manure P was extracted at dry matter–to–water extraction ratios of 0.5 g/200 mL, 2 g/200 mL, 2 g/20 mL, and 20 g/200 mL. Shaking times of 0.5 h, 1 h, or 2 h were evaluated along with filter paper types (Whatman No. 42, Whatman No. 40, and 0.45‐µm). Single or sequential extractions and repeated extractions with water or NaHCO3 were also compared on various manure sources. Manure types were treated as replications in the analysis of variance to reduce the probability of making a Type I error in applying the results to diverse manure types. Dry matter–to–water extraction ratios more concentrated than 1 g/100 mL removed less P than extraction at 1 g/200 mL, which removed a similar percentage of total P (TP) as 0.5 g/200 mL ratio. A single extraction with a 1 g/200 mL or more dilute ratio with 1 h of shaking time was found to give a good estimate of extractible P. Extracted manure P was similar for three sequential extractions of 1 g/100 mL dilution ratio compared to one extraction with 1 g/300 mL. Filter paper type did not affect the amount of P extracted. Phosphorus extraction was more consistent with samples stored dry as compared to refrigerated or frozen conditions. Extractible P in swine manure, as a percentage of TP, was more than for other manure types.  相似文献   

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