共查询到18条相似文献，搜索用时 15 毫秒
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脂联素（Adp）是主要由脂肪组织分泌的细胞因子,有重要的生理作用。本试验旨在研究重组脂联素（rAdp）对皖南花猪脂肪细胞脂联素及其受体2,AMP激活蛋白激酶（AMPK）、过氧化物增殖剂活化受体α（PPARα）mR-NA表达的影响。选择10d皖南花猪皮下脂肪组织分离前体脂肪细胞,增殖培养至80%融合后换分化培养基培养,细胞分化后用0、2和10mg/L rAdp分别处理12和48h。油红O染色法鉴定脂肪细胞,MTT方法检测细胞活力;酶法测定培养液中甘油释放量,荧光定量RT-PCR方法检测脂肪细胞脂联素（Adp）、脂联素受体1（AdpR1）、脂联素受体2（AdpR2）、PPARα和AMPK mRNA表达。结果显示,rAdp处理后,脂肪细胞活力总体有降低趋势,10mg/L处理48h达到显著水平（P〈0.05）;rAdp处理对甘油释放的抑制作用未达到差异水平。rAdp处理12h后,脂肪细胞AdpR1和AdpR2mRNA表达显著升高（P〈0.01）,但无剂量依赖性;rAdp处理48h后,脂肪细胞AdpmRNA表达显著下降（P〈0.05）。rAdp处理12h后,脂肪细胞PPARαmRNA表达显著升高（P〈0.01）,且有剂量效应性;而AMP AMPK mRNA表达均无显著性变化。结果提示,重组脂联素处理猪原代脂肪细胞有降低细胞活力和抑制脂肪细胞甘油释放量的趋势,能显著上调AdpR1、AdpR2和PPARα基因的表达,从而刺激脂肪酸氧化和甘油三酯的水解作用。 相似文献
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肠道是动物体重要的消化和免疫器官，具有为机体提供必要的营养、参与机体正常的免疫功能等作用。家禽的部分肠道疾病（如鸡白痢、禽伤寒、禽副伤寒）多与炎症反应有关，能降低家禽对饲料的利用率，严重者甚至会造成家禽死亡，对经济效益产生负面影响。脂联素是主要由脂肪细胞分泌的一种激素类物质。近年来的研究发现，肠上皮细胞也可以分泌脂联素，且肠内各种细胞均有其受体的表达。文章综述了家禽肠道炎症危害及其诱因、脂联素的抗炎机制，进而阐明脂联素在肠炎发生、发展中的作用及机制，以期为家禽肠道疾病的防治提供更多的思路和理论依据。 相似文献
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脂联素(adiponectin)是一种能参与调控糖和脂质代谢、能量调节、免疫反应以及抵抗炎症、氧化应激和细胞凋亡等多种生理过程的激素。禽脂肪性肝病是由于禽肝脏中脂肪酸合成与酯化、脂肪酸转运、脂肪酸氧化、脂肪分解和利用等代谢途径发生异常, 导致的脂类代谢紊乱、肝细胞脂肪变性和炎症反应。文章介绍了脂联素及其分子结构, 脂联素受体及其参与的信号通路, 并重点阐述了脂联素对肝脏脂质代谢、炎症、氧化应激、肝细胞凋亡与自噬方面的调节作用及机制, 以及脂联素受体激动剂的生理作用。同时, 结合禽脂肪性肝病的发生发展机制, 对脂联素及其受体激动剂的应用前景进行了展望。文章为预防和治疗禽类乃至其他动物的脂肪性肝病提供了新的思路。 相似文献
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脂联素是一种由脂肪组织分泌的具有多种生物学功能的特殊蛋白,在增强脂肪酸氧化、抗炎症反应、抗糖尿病等方面起重要作用。脂联素通过AdipoR1和AdipoR2这两种受体的介导经过AMPK、PPAR、p38MAPK等信号通路来发挥生物学作用。脂联素及其受体AdipoR1和AdipoR2能在多种组织器官中表达,AdipoR1主要在肌肉组织中表达,AdipoR2则高表达于肝脏组织。此外,脂联素及其受体还能在下丘脑、垂体、子宫、胚胎等多种生殖腺和生殖组织中表达,说明脂联素在调控动物生殖及胚胎生长发育方面起重要作用。 相似文献
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脂联素及其受体对脂代谢信号转导通路的调控机制 总被引：1，自引：0，他引：1
脂联素(Adipo Q)是一种由脂肪组织分泌的细胞因子,在调节畜禽的脂代谢等方面具有重要作用。Adipo Q主要通过与脂联素受体1(Adipo R1)和脂联素受体2(Adipo R2)2种受体结合来调控腺苷酸活化蛋白激酶α(AMPKα)、p38丝裂原激活蛋白激酶(p38MARK)和过氧化物酶体增殖物激活受体α(PPARα)等信号转导通路,参与机体内的脂代谢途径。目前对Adipo Q介导的脂代谢信号转导通路的研究已有了一定进展。本文就Adipo Q及其受体的结构,以及Adipo Q及其受体对脂代谢的调控机制进行了综述。 相似文献
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孙永刚 《青海畜牧兽医杂志》2014,(3):45-46
<正>脂联素(adiponectin)是脂肪细胞分泌、唯一随脂肪含量的增加而表达下降的细胞因子,它与受体在能量分配、贮存和消耗以及疾病方面起着不可忽视的作用~([1,2])。脂联素通过结合脂联素受体(adiponectin receptors)可以提高糖、脂的代谢,使动物向不利于脂肪形成的方向发展。同时,不同组织脂联素受体的分布不同,脂联素受体的种类与脂联素的结合力和敏感性密切相关,而且脂联素可能是通过不同的受体发挥其在相应组织的功能~([3])。本文就脂联素及其受体的结构特征、脂联素及其受体表达调控以及对脂肪代谢的作用做一阐述。 相似文献
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脂联素（AdipoQ）是一种主要由脂肪组织分泌的内源性细胞因子,在调节脂类代谢中发挥重要作用。AdipoQ首先与其受体[脂联素受体1(AdipoRl)和脂联素受体2(AdipoR2)]位于膜外的C端结合,再通过AdipoQ受体N端与信号接头蛋白结合,进而激活下游腺苷酸活化蛋白激酶（AMPK）与过氧化物酶体增殖物激活受体α(PPARα)等多条信号通路,促进脂肪酸氧化,抑制脂质合成,从而调节脂类代谢。本文就AdipoQ及其受体的结构、信号接头蛋白和AdipoQ对动物脂质代谢的调节机制进行综述。 相似文献
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脂多糖（LPS）是革兰阴性菌细胞壁的主要成分,广泛存在于环境中,可诱导机体炎症反应,与奶牛的多种疾病相关。环境和疾病等因素引起机体LPS水平升高,LPS与巨噬细胞、中性粒细胞和上皮细胞等作用,激活NF-κB和MAPKs信号通路,释放炎症因子,改变机体糖脂代谢相关激素和脂肪因子水平,进而影响糖脂代谢,造成奶牛2型糖尿病、酮病、脂肪肝和肥胖等代谢性疾病。本文综述了LPS与炎症反应和糖脂代谢相互作用关系及其导致糖脂代谢异常作用机制,为LPS致奶牛糖脂代谢异常机理研究提供参考。 相似文献
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Stimulation of lipogenesis by insulin in swine adipose tissue: antagonism by porcine growth hormone 总被引：1，自引：0，他引：1
The effects of physiological (1, 10 ng/ml) and pharmacological (1,000 ng/ml) concentrations of insulin (INS) and porcine growth hormone (pGH) on lipid metabolism were determined in short-term (2 h) and long-term (26, 50 h) incubations of swine adipose tissue. The short-term effects of three different commercial sources of bovine serum albumin (BSA) on adipose tissue metabolism were also evaluated. Two of the three BSA preparations were found to be unsuitable for inclusion in the short-term incubation buffer because they caused a stimulation of lipid synthesis in adipose tissue and masked the stimulatory effects of insulin. Physiological concentrations of insulin stimulated glucose metabolism in 2-h incubations by 100% in adipose tissue from 80-kg swine. After a 26-h incubation period, INS maintained rates of glucose metabolism at levels comparable to maximally stimulated rates in fresh tissue. Insulin also enhanced glucose metabolism following 50-h incubations; however, rates were less than for 2- or 26-h incubations. Glucose metabolism was also stimulated in adipose tissue from 127-kg swine when incubated for 2 h with INS; however, INS responsiveness declined with increasing body weight. Lipogenesis and glucose oxidation were partially maintained by INS using tissue from the heavier swine. A pharmacological but not physiological concentration of pGH stimulated glucose metabolism in short-term incubations by 50% in adipose tissue from 80-kg swine, and by 10% in adipose tissue from 127-kg swine. Long-term culture of adipose tissue in the presence of pGH had no effect on glucose metabolism. Physiological levels of pGH directly antagonized the stimulation of glucose metabolism by INS in short- and long-term incubations. In summary, these results are the first to establish that swine adipose tissue is quite sensitive to insulin and that pGH directly antagonizes insulin action. 相似文献
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脂肪酸在动物脂质代谢和免疫调节中起着至关重要的作用,G蛋白偶联受体120(G-protein coupled receptor 120,GPR120)又被称为游离脂肪酸受体4(free fatty acid receptor 4,FFAR4)是G蛋白偶联受体家族成员。它可以被长链脂肪酸激活,参与调节机体糖脂代谢,是治疗人及畜禽脂质代谢紊乱及免疫调节等相关疾病的热点靶点。本文就近来GPR120在脂肪组织中的研究现状、对免疫调节的作用及其激动剂或配体作为药物运用的可能做一综述。 相似文献
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OBJECTIVE: To examine the effect of darglitazone, a compound of the thiazolidinedione class, on glucose clearance and lipid metabolism in obese cats. ANIMALS: 18 obese and 4 lean adult neutered female cats. PROCEDURE: IV glucose tolerance tests with measurements of glucose, insulin, and nonesterified fatty acid (NEFA) concentrations were performed before and 42 days after daily administration of darglitazone (9 obese cats) or placebo (9 obese and 4 lean cats). Additionally, cholesterol, triglyceride, leptin, and glycosylated hemoglobin concentrations were measured. RESULTS: Darglitazone-treated cats had significantly lower cholesterol, triglyceride, and leptin concentrations, compared with placebo-treated obese cats. A significant decrease in the area under the curve for NEFAs, glucose, and insulin during an i.v. glucose tolerance test was seen in darglitazone-treated cats. The drug was well tolerated. CONCLUSION AND CLINICAL RELEVANCE: The response of obese cats to darglitazone was similar to the response to thiazolidinediones in obese humans and rodents Darglitazone was effective in improving insulin sensitivity and glucose and lipid metabolism in obese cats. 相似文献
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Leucine metabolism in comparison to glucose, and with substrate and insulin supplementation, were studied in bovine adipose tissue slices obtained from the tailhead region. In addition, leucine metabolism by isolated adipocytes in a Krebs-Ringer bicarbonate (KRB) buffer was compared to metabolism in Medium-199. Slices oxidized leucine and incorporated the amino acid into cellular protein and lipid, though at much lower rates than for glucose. Glucose addition increased leucine oxidation and its incorporation into lipid but did not affect protein synthesis. Insulin, up to 100 ng/ml, had no effect. Isolated cells convened a higher proportion of the leucine utilized to lipid, and less to protein, than did slices. Absolute rates of oxidation and lipid synthesis were lower, and protein synthesis higher, for Medium-199 than for KRB. Of the total leucine utilized, conversion to lipid represented the largest percentage in both buffers. Insulin had no effect in either buffer system. Bovine adipose tissue, the major site of fatty acid synthesis in this species, was found to both oxidize leucine, and utilize the amino acid for synthesis of cellular components. The isolated adipocyte, free of connective tissue, directs this “ketogenic” amino acid primarily towards lipid synthesis, by mechanisms which appear to be insulin insensitive in the adult bovine, as studied under short-term, in-vitro conditions. 相似文献
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脂联素是脂肪组织细胞特异性分泌的一种细胞因子,能够调控生物体的能量稳态、糖类和脂类代谢、肥胖、抵抗炎症反应等,具有多种生物学功能,在人类健康方面发挥着重要作用,同样对脂联素的深入研究也对我们在畜牧业生产中如何降低畜禽脂肪沉积,改善肉品质等方面有重要的指导作用。文中就脂联素的来源、结构,对糖和脂肪代谢调控、表达及其受体的研究进展作一综述。 相似文献