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1.
绵羊MyoG基因外显子1的多态性及其与肉质性状的关联分析   总被引：1，自引：0，他引：1  
肌细胞生成素（myogenin,MyoG）基因在肌细胞分化过程中起着中心调节作用,直接影响着动物的产肉能力。本研究以大尾寒羊、小尾寒羊、豫西脂尾羊、兰州大尾羊、蒙古羊、同羊6个绵羊品种为试验材料,利用非变性聚丙烯酰胺凝胶电泳检测MyoG基因外显子1的多态性,并与绵羊的肉质性状进行关联分析,探讨MyoG基因外显子1对绵羊肉质性状的影响。结果表明,MyoG基因外显子1在6个绵羊群体中均检测到3种基因型（AA、BB、AB）和2个等位基因（A、B）,在小尾寒羊、大尾寒羊、豫西脂尾羊、兰州大尾羊、蒙古羊、同羊群体中检测到MyoG基因外显子1的A等位基因频率分别为：0.5167、0.2500、0.4375、0.6500、0.5750和0.7125,MyoG基因外显子1的B等位基因频率分别为：0.4833、0.7500、0.5625、0.3500、0.4250和0.2875。MyoG基因外显子1主要对羊肉的水分和色泽有影响,主要表现为BB基因型水分极显著高于AB基因型（P< 0.01）,显著高于AA基因型（P< 0.05）;AB基因型的色泽极显著高于BB基因型（P< 0.01）,显著高于AA基因型（P< 0.05）。  相似文献   

2.
Myogenin (MyoG) gene plays a central regulatory role in the process of muscle cell differentiation, which directly affects meat production capacity of animal. In this study, 6 sheep varieties including Large-tail Han sheep, Small-tail Han sheep, Yuxizhiwei sheep, Lanzhou fat-tailed sheep, Mongolia sheep and Tong sheep were selected as experimental materials to detect the polymorphism of MyoG gene exon 1 by non denaturing polyacrylamide gel electrophoresis, and analyze the association analysis between the polymorphism of MyoG gene exon 1 and meat quality traits in sheep. The results showed that 2 alleles (A, B) and 3 genotypes (AA, BB, AB) were detected in MyoG gene exon 1 of six sheep populations. The A allele frequency in Small-tail Han sheep, Large-tail Han sheep, Yuxizhiwei sheep, Lanzhou fat-tailed sheep, Mongolia sheep and Tong sheep were 0.5167, 0.2500, 0.4375, 0.6500, 0.5750 and 0.7125, respectively, the B allele frequency of six sheep populations were 0.4833, 0.7500, 0.5625, 0.3500, 0.4250 and 0.2875, respectively. MyoG gene exon 1 mainly affected the moisture and color of mutton, the moisture content of BB genotype was significantly higher than that of AB genotype (P< 0.01) and AA genotype (P< 0.05). The color of AB genotype was significantly higher than that of BB genotype (P< 0.01) and AA genotype (P< 0.05).  相似文献   

3.
为研究钙蛋白酶抑制蛋白（calpastatin,CAST）基因多态性与新疆阿勒泰羊肉嫩度的关系,本试验以89只新疆阿勒泰羊为研究对象,运用PCR-SSCP方法对新疆阿勒泰羊CAST基因第1、2、21外显子进行多态性检测,采用DNA测序技术对结果进行测序,并与新疆阿勒泰羊肉嫩度进行关联性分析。结果显示,在第1和2外显子均未发现多态性,在第21外显子检测出AA、AB、BB 3种基因型,测序结果显示该处发生了A→G的突变。关联分析结果表明,该突变对阿勒泰羊肉嫩度有显著影响（P＜0.05）,初步推断AA基因型可能对阿勒泰羊肉嫩度有一定的改善作用。  相似文献   

4.
试验旨在研究LAMB1基因外显子在细毛羊中的多态性及其与羊毛纤维直径的关联性。基于DNA池重测序技术获得的SNPs数据,共筛选LAMB1基因外显子区域20个SNPs,利用直接测序法、PCR-SSCP及生物信息学软件对10个错义突变SNPs的准确性进行验证,利用SAS 8.1的GLM程序分析其对新疆巩乃斯种羊场育种核心群300只细毛羊的遗传效应及与被毛纤维直径的关联性。结果表明,20个SNPs中10个是同义突变SNPs;10个错义突变SNPs验证后7个发生错义突变,导致蛋白性质改变,3个呈假阳性。突变后LAMB1蛋白疏水性更强,预测是不可溶性蛋白。SNP5中TT基因型个体纤维直径显著高于TC和CC基因型个体（P<0.05）,SNP9中GG和TT基因型个体纤维直径显著高于GT基因型个体（P<0.05）。虽然DNA混池全基因组重测序技术完整地检测到基因的SNPs,并将假阳性最小化,但还需要对结果进行验证。研究揭示LAMB1基因外显子多态性丰富,突变SNPs在外显子中分布不平衡,LAMB1基因SNP5（rs159769941）和SNP9（rs159769901）可以考虑作为影响细毛羊被毛纤维直径的有效遗传标记。  相似文献   

5.
This study aimed to research the single nucleotide polymorphism (SNPs) of LAMB1 gene exon and its correlation with the fiber diameter in Fine-wool sheep.Based on DNA pools with the Re-sequencing technology to gain SNPs data,totally screened 20 SNPs of LAMB1 gene exon regions,at the same time,combining with direct sequencing method,PCR-SSCP and bioinformatics software to verify the accuracy of 10 missense mutations.The genetic effects of LAMB1 on fiber diameter at Xinjiang Kunes farm were analyzed by the GLM of SAS,totally 300 sheep.The results showed that 20 SNPs of LAMB1 gene were screened,which included 10 synonymous mutation SNPs and 10 non-synonymous mutation SNPs,after the validation,there were 7 missense mutation SNPs which led to the nature of protein change,3 SNPs were not mutated.LAMB1 protein became increasingly hydrophobic after mutating,predicting that was insoluble protein.TT genotype in the fiber diameter of the individual value was significantly higher than TC and CC genotypes on SNP5(P<0.05),GG and TT genotypes in the fiber diameter of the individual value was significantly higher than GT genotype on SNP9 (P<0.05).Although,all of the SNPs were completely detected by DNA pool with the Re-sequencing technology and minimized the false positives,it still need to be validated by direct sequencing.The results revealed that LAMB1 gene existed highly genetic diversity,mutated SNPs were unevenly distributed in exons,SNP5(rs159769941) and SNP9(rs159769901) could be considered as an effective genetic markers of Fine-wool sheep on fiber diameter.  相似文献   

6.
利用PCR-SSCP和DNA测序法,首次检测了湖羊、小尾寒羊、洼地绵羊、阿勒泰羊中的HAS2基因,并对该基因第2和第3外显子部分单核苷酸多态性进行分析。结果表明:湖羊、小尾寒羊、洼地绵羊、阿勒泰羊在第2外显子中存在3种基因型,各是pp、pq、qq基因型;在第3外显子中存在2种基因型,为rr、rs基因型;HAS2基因第2外显子中发生1处C→A突变,第3外显子中发生1处A→G突变。经χ2适合性检验,4种绵羊在HAS2基因第2外显子上的基因型分布均不符合Hardy-Wenberg平衡,在第3外显子上的基因型分布全符合Hardy-Wenberg平衡。各基因型频率在4种绵羊间的分布均没有显著差异(P0.05);湖羊2个位点上的所有基因型在产羔数上差异均不显著(P0.05)。初步认为HAS2基因不能作为湖羊高繁殖力的候选基因。  相似文献   

7.
为探索胰高血糖素样肽-2受体（glucagon-like peptide-2 receptor，GLP2R）基因多态性与绵羊肉质性状的相关性，试验选用68只南非肉用美利奴羊×东北细毛羊和102只杜泊羊×小尾寒羊的杂交后代为研究对象，采用Sanger测序方法寻找GLP2R基因外显子11序列的突变位点，根据测序结果分析该突变位点的基因型频率和基因频率及卡方适合性检验、纯合度（Ho）、杂合度（He）和多态信息含量（PIC）。结果显示，绵羊GLR2R基因外显子11处存在C/T突变，存在3种基因型：CC、TC和TT，2种等位基因：C和T，其中在南非肉用美利奴羊×东北细毛羊群体中，TC基因型为优势基因型，在杜泊羊×小尾寒羊群体中，CC基因型为优势基因型；2个群体中C均为优势等位基因。卡方适合性检验结果显示，2个群体的C/T位点均处于Hardy-Weinberg平衡状态（P>0.05）；C/T突变位点在群体内变异较小且处于中度多态（0.25 < PIC < 0.5），有一定遗传学意义。不同基因型肉质性状差异结果显示，南非肉用美利奴羊×东北细毛羊TC基因型剪切力显著高于CC和TT基因型，TT基因型失水率显著高于CC和TC基因型（P<0.05）；杜泊羊×小尾寒羊CC基因型剪切力显著高于TC和TT基因型，TT基因型失水率显著高于CC和TC基因型（P<0.05）。本研究在GLP2R基因外显子处发现C/T突变，可能是影响肉质性状的潜在位点，是否能够作为遗传标记还需要进一步探索。  相似文献   

8.
The purpose of this experiment was to study the correlation between exon 1 and 4 polymorphisms of DRB1 gene and brucellosis in Kazakh sheep.Using RBPT serological tests to try sheep serum,reference in GenBank sheep MHC Class Ⅱ area DRB1 gene sequences (Accession No.:NC_040271.1),the exon 1 and 4 pieces designed primers,using PCR-SSCP and DNA sequencing technology to 230 Kazak sheep DRB1 gene polymorphism detection,analyze its polymorphism loci and the relationship between the Kazak sheep Brucella susceptibility.The results showed that 66 Kazakh sheep were positive for Brucella in RBPT test,and the positive detection rate was 28.7%.There was one SNP locus (F1-G22A) in exon 1 fragment,and sequencing determined two genotypes (GG and GA),the dominant allele and genotype were G and GG respectively,and the susceptibility genotype of the polymorphisms of F1-G22A was GA.Chi-square test showed that there was no significant correlation between the polymorphisms of DRB1 gene F1-G22A and Brucella susceptibility in Kazakh sheep (P>0.05).According to the analysis of bioinformatics online software,the F1-G22A polymorphic sites lead to the change of RNA secondary structure and the decrease of minimum free energy,and lead to the change of protein secondary structure.No SNPs were found in DRB1 exon 4 fragment.Therefore,there might be a certain correlation between the polymorphisms of DRB1 gene F1-G22A and Brucella susceptibility in Kazakh sheep.  相似文献   

9.
试验旨在对豫西脂尾羊诱导细胞凋亡DNA片段化因子45样效应因子A （cell death-inducing DNA fragmentation factor 45-like effectors A，CIDEa）基因进行克隆及生物信息学分析，并检测其在豫西脂尾羊不同组织中的表达。以豫西脂尾羊脂肪组织RNA为模板，采用RT-PCR方法扩增并克隆豫西脂尾羊CIDEa基因完整CDS区序列，对测序结果进行了相关生物信息学分析，并对CIDEa基因在豫西脂尾羊心脏、肝脏、肺脏、脾脏、肾脏、瘤胃、小肠、背最长肌、皮下和内脏脂肪组织中的表达进行了分析。结果显示，豫西脂尾羊CIDEa基因CDS区序列长660 bp，编码219个氨基酸；豫西脂尾羊与绵羊、水牛、黄牛、藏羚羊、猪和人的CIDEa基因同源性分别为99.1%、96.8%、96.2%、98.8%、85.0%和79.4%。蛋白理化性质分析表明，CIDEa蛋白分子质量为24.38 ku，理论等电点（pI）为9.12，属于碱性蛋白。跨膜结构和信号肽预测分析表明，CIDEa蛋白不含跨膜结构和信号肽。亚细胞定位分析表明，豫西脂尾羊CIDEa蛋白分布在细胞质（47.8%）、线粒体（26.1%）、细胞核（17.4%）、液泡（4.3%）和内质网（4.3%）。蛋白质三级结构预测发现，CIDEa蛋白结构具有2个α-螺旋、4个β-折叠及一些无规则卷曲。实时荧光定量PCR结果表明，CIDEa基因在豫西脂尾羊脂肪组织（皮下和内脏脂肪组织）中表达量较高，其他组织中表达量从高到低依次为小肠、肝脏、脾脏、瘤胃、心脏、肾脏、肺脏、背最长肌。这些结果可为进一步研究CIDEa基因在肉羊脂质代谢中的功能及羊肉品质调控提供基础资料。  相似文献   

10.
马雪珍  徐杰  高剑峰  李刚 《中国畜牧兽医》2020,47(12):3844-3851
试验旨在对哈萨克绵羊DRB1基因外显子1和4多态性与布鲁氏菌病的相关性进行研究。使用虎红平板凝集试验（RBPT）对试羊的血清进行血清学检测，参考GenBank中绵羊MHC ClassⅡ区DRB1基因序列（登录号：NC_040271.1），对其外显子1和4片段设计引物，采用PCR-SSCP和DNA测序技术对230只哈萨克绵羊的DRB1基因进行多态性检测，分析其多态位点与哈萨克绵羊布鲁氏菌易感性之间的关系。RBPT检测发现66只哈萨克绵羊为布鲁氏菌感染阳性，阳性检出率为28.7%。外显子1片段存在一个SNP位点（F1-G22A），测序确定两种基因型（GG、GA），优势等位基因和基因型分别为G、GG，F1-G22A多态位点的易感基因型为GA。卡方检验表明，哈萨克绵羊DRB1基因F1-G22A多态位点与布鲁氏菌易感性的相关性不显著（P>0.05）。通过生物信息学在线软件分析得出，F1-G22A多态位点导致了RNA二级结构的改变和最小自由能的降低，引起了蛋白质二级结构的改变。DRB1基因外显子4片段未发现SNPs。由此得出，哈萨克绵羊DRB1基因F1-G22A多态位点与布鲁氏菌易感性可能存在一定的相关性。  相似文献   

11.

Purpose  

The purpose of this study was to evaluate the brain, renal, and hepatic effects of three NSAIDs (flunixin meglumine, ketoprofen, and phenylbutazone) when administered IV to clinically normal Iranian fat-tailed sheep.  相似文献   

12.
试验旨在研究Lpin2基因遗传变异对绵羊尾型和屠宰性状的影响。以两品种脂尾型绵羊广灵大尾羊和小尾寒羊为研究对象,采用DNA直接测序法检测Lpin2基因5'非编码区部分序列的单核苷酸多态性（SNPs）,并分析其与尾型及屠宰性状的关联性。结果发现,Lpin2基因5'非编码区起始密码子上游约1 200 bp的DNA序列中存在3个SNPs,即NC_019480.2:g.－663 dup ATT（SNP1）、g.－388 T > C（SNP2）和g.－330 T > G（SNP3）,其中SNP1为三核苷酸ATT重复扩展短串联重复序列（STR）的拷贝数变异（CNV）,在广灵大尾羊中的突变频率显著高于小尾寒羊（P<0.05）,显著降低了广灵大尾羊的胴体重与屠宰率（P<0.05）,对小尾寒羊的屠宰性状无显著影响（P>0.05）。SNP2和SNP3构成单倍型块,形成的单倍型对各性状无显著影响（P>0.05）。试验结果可为绵羊肉质性状的标记辅助选择提供参考依据。  相似文献   

13.
为探寻湖羊IGF-1基因5′侧翼区多态性及其对羊毛弯曲度性状的影响,选用128只湖羊作为实验材料,对IGF-1基因5'侧翼区进行扩增、测序和PCR-SSCP检测,探讨IGF-1基因5′侧翼区的遗传特征,寻找与羊毛弯曲度性状相关的遗传标记,为湖羊羊毛弯曲度标记辅助选择提供理论依据。结果表明:湖羊IGF-1基因5'侧翼区突变位点有AA、AB、BC三种基因型,基因型频率分别为0.609 4、0.093 8和0.296 8。AB型与AA型相比,在外显子1前的第649 bp有一处G→A突变,BC型与AA型相比,在外显子1前的第651 bp处发生G→C突变,在外显子1前的第649 bp有一处G→A突变。卡方独立性检验结果表明,不同基因型个体羊毛花纹类型和基因型之间不存在显著相关关系(P>0.05)。该研究初步认为,IGF-1基因5'侧翼区多态性与羊毛弯曲度性状之间没有显著关联(P>0.05)。  相似文献   

14.
A total of 167 sheep belonging to the Estonian whiteheaded mutton, Estonian blackheaded mutton, Lithuanian coarsewool native, Lithuanian blackface and Latvian darkheaded mutton breeds, and a population of sheep kept isolated on the Estonian island of Ruhnu, were sequence-analysed for polymorphisms in the prion protein (PrP) gene, to determine their genotype and the allele frequencies of polymorphisms in PrP known to confer resistance to scrapie. A 939 base pair fragment of exon 3 from the PrP gene was amplified by pcr and analysed by direct sequencing. For animals showing polymorphism at two nucleotide positions, both haplotypes of these double-heterozygous genotypes were further verified by pcr cloning and sequence analysis. Known polymorphisms were observed at codons 136, 154 and 171, and six different haplotypes (arr, ahq, arh, ahr, arq and vrq) were determined. On the basis of these polymorphisms, the six populations of sheep possessed the resistant arr haplotype at different frequencies. The high-risk arq haplotype occurred in high frequencies in all six populations, but vrq, the haplotype carrying the highest risk, occurred at low frequencies and in only three of the populations.  相似文献   

15.
This study was aimed to explore the effects of genetic variations of Lpin2 gene on tail type and slaughter traits in sheep. Two breeds of fat-tailed sheep such as Guangling Large Tailed sheep and Small Tailed Han sheep were chosen to identify the single nucleotide polymorphisms (SNPs) in 5' non-coding region of Lpin2 gene by using direct DNA sequencing, and the associations between SNPs and tail type and slaughter traits were analyzed. The results showed that three novel SNPs were found in 5' non-coding region about 1 200 bp upstream sequence from start codon ATG in Lpin2 gene, namely NC_019480.2: g.－663 dup ATT (SNP1), g.－388 T > C (SNP2) and g.－330 T > G (SNP3), sequencing data revealed that the trinucleotide ATT repeat expansion at SNP1 was cope number variation (CNV) of short tandem repeats (STR), the mutation frequency was significant higher in Guangling Large Tailed sheep than that in Small Tailed Han sheep (P<0.05), and caused the significant decreasing in carcass weight and dressing percentage in Guangling Large Tailed sheep (P<0.05), however this variation exhibited non-significant effects on the slaughter traits in Small Tailed Han sheep (P>0.05). SNP2 and SNP3 could be constructed into a haplotype block while the haplotypes didn't significantly affect the examined traits in two sheep breeds. This finding would provide references for marker assisted selection of the sheep meat quality traits.  相似文献   

16.
Genetic parameters for average daily gain from birth to weaning (ADGa), birth to 6 months (ADGb), weaning to 6 months (ADGc), weaning to yearling age (ADGd), and corresponding Kleiber ratios (KRa, KRb, KRc, and KRd) were estimated by using records of 3,533 Zandi lambs, descendent of 163 sires and 1265 dams, born between 1991 and 2005 at the Zandi Sheep Breeding Station at Khojir National Park, Tehran, Iran. A derivative-free algorithm combined with a series of six single-trait linear animal models was used to estimate phenotypic variance and its direct, maternal, and residual components. In addition, bivariate analyses were done to estimate (co)variance components between traits. Estimates of direct heritability (h 2 ) were 0.11, 0.15, 0.09, 0.10, 0.10, 0.10, 0.06, and 0.07 for ADGa, ADGb, ADGc, ADGd, KRa, KRb, KRc, and KRd, respectively, thereby indicating the presence of low additive genetic variation for growth rate and Kleiber ratio in this population of Zandi sheep. Maternal genetic component was found to be significant on ADGa and KRa and contributed 3% and 5%, respectively, in total phenotypic variance of ADGa and KRa. A widespread range of genetic correlations among traits studied was observed. Except for negative genetic correlations between ADGa and KRc, ADGa and KRd, and between KRa and KRc, in other cases, genetic correlations were positive and moderate to very high. Phenotypic correlations ranged from −0.49 (ADGa/KRd) to 0.94 (ADGc/KRc). These results indicate that selecting for improved growth rate or Kleiber ratio in Zandi sheep would generate a relatively slow genetic progress.  相似文献   

17.
试验旨在研究肌肉生长抑制素(myostatin,MSTN)基因多态性及其对东佛里生羊生长性状的影响。从324只东佛里生羊全血中提取DNA,对MSTN基因外显子序列和非翻译区(untranslated region,UTR)序列进行PCR扩增,采用一代测序技术鉴定单核苷酸多态性(single nucleotide polymorphism,SNP)位点,分析不同位点的遗传多态性,并将不同基因型与东佛里生羊的生长性状进行关联分析。结果显示,在东佛里生羊MSTN基因外显子中未检测到突变;在3'-UTR区的272 bp处存在G→A单碱基突变,该位点只检测到AG和AA基因型,且AA基因型频率高于AG基因型;在5'-UTR区的176 bp处存在C→A突变,该位点只检测到CC和AC基因型,且CC基因型频率高于AC基因型。遗传多样性参数分析结果表明,东佛里生羊群体MSTN基因3'-UTR-272和5'-UTR-176位点的纯合度分别为0.827和0.887,杂合度分别为0.173和0.113,2个位点的多态性均较低(PIC<0.25),其群体基因型分布均符合哈代-温伯格平衡(P>0.05)。关联分析发现,3'-UTR-272位点AG基因型体斜长显著高于AA基因型(P<0.05),5'-UTR-176位点CC基因型胸围显著高于AC基因型(P<0.05),其余指标均无显著差异(P>0.05)。综上,东佛里生羊MSTN基因3'-UTR和5'-UTR突变位点可作为其目标性状选育的分子标记。  相似文献   

18.
The objective of this study was to investigate association between GDF9 and BMP15 gene polymorphism and litter size in fat-tailed sheep, a total of 97 mature ewes from four breeds (Afshari=19; Baluchi=18; Makui=30 and Mehraban=30) were genotyped for the BMP15 HinfI and GDF9 HhaI polymorphisms by PCR-RFLP technique. The highest and lowest mutant allele frequencies were found in Makui (0.27) and Afshari (0.10) sheep for the BMP15 gene and in Afshari (0.24) and Mehraban (0.18) sheep for the GDF9 gene, respectively. Litter size was significantly influenced by genotype of the ewe for two genes (P < 0.01). Heterozygous genotypes for both loci showed higher litter size than homozygous genotypes (P < 0.01). None of the individuals carried homozygous genotype for both of the GDF9 and BMP15 variants in these breeds. The individuals carrying the mutant allele for one of the investigated candidate gene still showed fertile phenotype. Thus, existence of homozygosity at one of the BMP15 and GDF9 variant is not probably able to block normal hormonal pathway of reproduction in fat-tailed sheep.  相似文献   

19.
The aim of the present study was to investigate the normal blood parameters of Iranian fat-tailed sheep (Zandi) and their changes due to rapid liver biopsy technique with a tru-cut biopsy needle. In ten ewe lambs, blood samples were collected from jugular vein and biopsy needle was inserted through the dorsal one third of the 11th intercostal space, on the right hand side of the lambs and liver specimen was collected. Physical examinations were performed on alternate days during the experiment. Blood collection was done on both before (day 1) and after (day 17) the biopsy. All animals were slaughtered at day 17. Values were compared using paired t test. While biopsy did not make any significant changes in mean values of body temperature, heart rate, respiratory rate, PCV, WBC, neutrophil, lymphocyte, eosinophil, monocyte, total serum protein, AST, ALT, and serum calcium (p > 0.05), it made a significant difference on the values of ALP (p < 0.001), serum inorganic phosphate (p = 0.035), and magnesium (p = 0.013). Necropsy examination revealed the points of hitting the biopsy needles on the diaphragmatic surface of the livers, surrounded by a zone of intense hyperemia. Peritoneal adhesions accompanying with typical strands of fibrous connective tissue between diaphragmatic surface of the liver and adjacent abdominal wall were found in two cases.  相似文献   

20.
为了分析微卫星标记ILSTS011的遗传多态性,研究以河南省地方绵羊品种大尾寒羊、小尾寒羊和豫西脂尾羊为研究对象,采用聚丙烯酰胺凝胶电泳技术分离微卫星标记的带型.结果表明:在3个绵羊品种中均检测到微卫星标记ILSTS011有3个等位基因,微卫星标记ILSTS011的平均杂合度为0.614 1,平均有效等位基因数为2.614 0,平均多态信息含量为0.551 3.说明ILSTS011为高度多态基因座,可作为有效的遗传标记用于大尾寒羊、小尾寒羊和豫西脂尾羊的遗传多样性分析.  相似文献   

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