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SLB9基因是BES1转录因子家族中的一员,BES1在调控油菜素内酯(BR)基因响应中发挥关键作用。研究证明BES1基因受多种胁迫诱导,介导BR信号,调节植物耐旱等抗逆性。采用基因沉默(VIGS)技术,通过农杆菌介导法构建SLB9基因沉默植株,干旱胁迫处理沉默植株,通过观察沉默植株与对照植株表型差异及生理生化指标变化,研究SLB9基因表达变化对番茄抗旱性的影响。结果表明,SLB9基因沉默植株抗旱性明显低于对照植株,推测SLB9基因下调表达降低番茄抗旱性。 相似文献
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Yi ZHANG Yu SHI Hai-jun GONG Hai-liang ZHAO Huan-li LI Yan-hong HU Yi-chao WANG 《农业科学学报》2018,17(10):2151-2159
Silicon can improve drought tolerance of plants,but the mechanism still remains unclear.Previous studies have mainly concentrated on silicon-accumulating plants,whereas less work has been conducted in silicon-excluding plants,such as tomato(Solanum lycopersicum L.).In this study,we investigated the effects of exogenous silicon(2.5 mmol L~(–1))on the chlorophyll fluorescence and expression of photosynthesis-related genes in tomato seedlings(Zhongza 9)under water stress induced by 10%(w/v)polyethylene glycol(PEG-6000).The results showed that under water stress,the growth of shoot and root was inhibited,and the chlorophyll and carotenoid concentrations were decreased,while silicon addition improved the plant growth and increased the concentrations of chlorophyll and carotenoid.Under water sterss,chlorophyll fluorescence parameters such as PSII maximum photochemical efficiency(F_v/F_m),effective quantum efficiency,actual photochemical quantum efficiency(Ф_(PSII)),photosynthetic electron transport rate(ETR),and photochemical quenching coefficient(q_P)were decreased;while these changes were reversed in the presence of added silicon.The expressions of some photosynthesis-related genes including PetE,PetF,PsbP,PsbQ,PsbW,and Psb28 were down-regulated under water stress,and exogenous Si could partially up-regulate their expressions.These results suggest that silicon plays a role in the alleviation of water stress by modulating some photosynthesis-related genes and regulating the photochemical process,and thus promoting photosynthesis. 相似文献
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Identification of the DEAD-box RNA helicase family members in grapevine reveals that VviDEADRH25a confers tolerance to drought stress 
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下载免费PDF全文 YANG Sheng-di GUO Da-long PEI Mao-song WEI Tong-lu LIU Hai-nan BIAN Lu YU Ke-ke ZHANG Guo-hai YU Yi-he 《农业科学学报》2022,21(5):1357-1374
Grapevine growing areas are increasingly affected by drought, which has greatly limited global wine production and quality. DEAD-box is one of the largest subfamilies of the RNA helicase family, and its members play key roles in the growth and development of plants and their stress responses. Previous studies have shown the potential of DEAD-box genes in the drought stress responses of Arabidopsis and tomato, rice, and other crop species. However, information about DEAD-box genes in grapevine remains limited. In this report, a total of 40 DEAD-box genes were identified in grapevine and their protein sequence characteristics and gene structures were analyzed. By comparing the expression profiles of VviDEADRHs in response to drought stress in different grapevine varieties, nine candidate genes (VviDEADRH10c, -13, -22, -25a, -25b, -33, -34, -36, and -39) were screened based on expression profiling data. Combined with qRT-PCR results, VviDEADRH25a was selected for functional verification. Heterologous overexpression of VviDEADRH25a in Arabidopsis showed the transgenic plants were more sensitive to drought stress than the control. Both electrolyte permeability and malondialdehyde content were significantly increased in transgenic plants, whereas the chlorophyll content and superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) enzyme activities were significantly decreased. Furthermore, VviDEADRH25a-overexpressing plants showed down-regulated expression levels of several drought stress-related marker genes, namely AtCOR15a, AtRD29A, AtERD15, and AtP5CS1, which indicated that they participated in the drought stress response. In summary, this study provides new insights into the structure, evolution, and participation of DEAD-box RNA helicase genes in the response to drought stress in grapevines. 相似文献
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Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways 下载免费PDF全文
下载免费PDF全文 While SlPti5 has been shown to play a crucial role in the regulation of antagonistic genes in Solanum lycopersicum and Arabidopsis against pathogen infection, there have been no comprehensive studies on the effects of SlPti5 on the regulatory response mechanism of reactive oxygen species (ROS) system and hormone pathways during growth and disease resistance of tomato plants. Here, we investigated the function of SlPti5 in the defense response of tomato against Botrytis cinerea utilizing a virus-induced gene silencing (VIGS)-based system. Expression profile analysis showed that SlPti5 was significantly induced upon B. cinerea infection, with high expression levels in the leaves and fruit of tomato. VIGS-based silencing of SlPti5 inhibited early vegetative growth, increased the plant's susceptibility to infection, promoted the development of ROS, affected the expression of genes involved in the ROS scavenging system, and attenuated the expression of genes associated with pathogenesis and the ethylene/jasmonic acid signaling pathways. In sum, our data demonstrated that SlPti5 stimulates the immune response of tomato plant to Botrytis cinerea infection by involving the ethylene (ET)- and jasmonic acid (JA)-mediated pathways and modulating the expression of some key pathogenesis-related (PR) genes. 相似文献
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以15%聚乙二醇6000(PEG-6000)处理模拟干旱胁迫,研究了氢气(H2)和脱落酸(ABA)在番茄(Solanum lycopersicum L.)幼苗抵抗干旱胁迫中的作用及其相互关系。结果表明,外源H2和ABA均可促进番茄在干旱胁迫下的生长,其中富氢水(HRW,H2供体)和ABA最适浓度分别为75%和150 μmol·L-1。干旱胁迫下,HRW处理能显著增加番茄幼苗叶片内源ABA含量及其关键基因表达。同时,HRW和ABA单独及其共同处理的番茄幼苗叶片丙二醛(MDA)含量较PEG处理分别降低了34.8%、32.6%和37.0%;而超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性及其基因表达显著高于PEG处理。另外,ABA抑制剂氟啶酮(FLU)抑制了HRW对干旱胁迫下番茄幼苗生长的促进作用,说明H2促进干旱胁迫下番茄幼苗的生长可能需要ABA的参与。FLU抑制了HRW对干旱胁迫下叶片MDA含量的降低作用。此外,FLU抑制了HRW对干旱胁迫下叶片SOD、CAT和APX的活性及其基因表达的增加作用,说明H2增强番茄幼苗抗氧化酶活性及其基因表达需要ABA的参与。可见,ABA参与了H2增强番茄幼苗的抗旱性,这一过程是通过抑制MDA积累和提高抗氧化酶活性及其基因表达来实现的。 相似文献
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TaNF-YB11, a gene of NF-Y transcription factor family in Triticumaestivum,confers drought tolerance on plants via modulating osmolyte accumulation and reactive oxygen species homeostasis 下载免费PDF全文
下载免费PDF全文 ZHAO Ying-jia ZHANG Yan-yang BAI Xin-yang LIN Rui-ze SHI Gui-qing DU Ping-ping XIAO Kai 《农业科学学报》2022,21(11):3114-3130
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谷子C2H2型锌指蛋白基因SiZFP182的克隆及表达分析 总被引：1，自引：0，他引：1
以‘晋谷45’和‘晋谷42’2个品种作为试验材料,采用聚乙二醇(PEG-6000)模拟干旱胁迫处理谷子幼苗,检测不同处理时间下丙二醛(MDA)的含量,评价其抗旱性。此外,在2个品种中克隆获得SiZFP182基因的cDNA全长,分析比较它们之间的序列差异;通过定量RT-PCR的方法分析SiZFP182基因在受到干旱胁迫的谷子幼苗中的表达特性。结果表明,‘晋谷45’的抗旱性强于‘晋谷42’,2个品种中SiZFP182基因的序列存在5处单碱基差异,使得编码的氨基酸序列不同。此外,PEG胁迫处理后24h内,SiZFP182基因在2个品种中的表达水平均表现出先升高后降低的趋势,处理后6~12h,该基因在‘晋谷45’中表达水平高于‘晋谷42’。初步认为‘晋谷45’的抗旱特性与‘晋谷42’对干旱敏感的特性,可能与干旱胁迫下处于幼苗期的2个品种中SiZFP182基因序列差异及表达差异相关。 相似文献
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为了探究 GbCDPK83基因在海岛棉响应干旱胁迫中的功能。利用PCR技术克隆 GbCDPK83基因，采用生物信息学方法分析GbCDPK83蛋白的理化性质、结构特征和在细胞中的位置，通过基因重组技术构建VIGS沉默载体并侵染棉花。本研究成功构建了沉默表达载体，沉默植株中 GbCDPK83的表达明显被抑制。干旱胁迫后， GbCDPK83沉默植株叶片比对照萎蔫更严重，相对含水量显著降低，相对电导率和丙二醛含量显著上升，脯氨酸含量升高但低于空载体及非转基因植株。沉默 GbCDPK83使海岛棉耐旱性减弱。 相似文献
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SlTPP4 participates in ABA-mediated salt tolerance by enhancing root architecture in tomato 下载免费PDF全文
下载免费PDF全文 《农业科学学报》2023,22(8):2384-2396
Salinity tolerance is an important physiological index for crop breeding. Roots are typically the first plant tissue to withstand salt stress. In this study, we found that the tomato (Solanum lycopersicum) trehalose-6-phosphate phosphatase (SlTPP4) gene is induced by abscisic acid (ABA) and salt, and is mainly expressed in roots. Overexpression of SlTPP4 in tomato enhanced tolerance to salt stress, resulting in better growth performance. Under saline conditions, SlTPP4 overexpression plants demonstrated enhanced sucrose metabolism, as well as increased expression of genes related to salt tolerance. At the same time, expression of genes related to ABA biosynthesis and signal transduction was enhanced or altered, respectively. In-depth exploration demonstrated that SlTPP4 enhances Casparian band development in roots to restrict the intake of Na+. Our study thus clarifies the mechanism of SlTPP4-mediated salt tolerance, which will be of great importance for the breeding of salt-tolerant tomato crops. 相似文献
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Influence of drought hardening on the resistance physiology of potato seedlings under drought stress
In this paper, the influence of drought hardening on the growth, development, resistance physiology, leaf microstructure and stomatal behavior of potato seedlings under drought stress was studied, and the mechanism of drought hardening improvement of potato seedling drought resistance was elucidated. We found that drought stress had several adverse effects on potato seedlings, yet drought hardening alleviated the decrease in relative water content (RWC), net photosynthetic rate (Pn) and chlorophyll content and inhibited the increase in relative electric conductivity and malondialdehyde (MDA) content. Compared with contrast seedlings, drought-hardened seedlings also had enhanced root vigor, increased antioxidant enzyme activity and higher levels of abscisic acid (ABA), proline (Pro), soluble sugars and polyamines (PAs) under drought stress. In addition, the stomatal density of potato seedling leaves increased significantly, while the leaf area, stomatal size and stomatal aperture decreased with drought hardening treatment. These changes led to reduced leaf transpiration rate (Tr) and improved water utilization efficiency (WUE). The changes in leaf microstructure also had a positive effect on the drought resistance of the drought-hardened potato seedlings. So it can be concluded that through increasing the content of some endogenous hormones, osmotic regulatory substances and the activities of antioxidant enzymes, the resistance physiology of drought-hardened potato seedlings was enhanced. 相似文献
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SLPIN6基因是番茄PIN家族一员,番茄PIN家族是一类生长素运输体蛋白家族,调控生长素极性运输.研究表明,干旱胁迫改变植物生长素极性运输平衡,影响植物抗旱性.团队前期研究发现SLPIN6基因在干旱胁迫时表达量显著上调,可能参与番茄植株抗旱应答调控.为进一步明确该基因抗旱调节功能,试验采用VIGS方法对SLPIN6基因作沉默处理,通过观察番茄植株抗旱表型及测定抗逆生理指标变化,发现SLPIN6基因沉默后番茄植株与对照相比,沉默番茄植株萎蔫程度加重,抗逆生理指标差异明显,表明SLPIN6基因参与番茄抗旱应答调控,具有抗旱调节功能. 相似文献
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前期研究已完成番茄WRKY转录因子家族分析,发现番茄WRKYⅡa和Ⅱb亚族基因多与抗逆相关,因此文章选取6个WRKYⅡa和Ⅱb亚族基因Sl WRKY13、Sl WRKY24、Sl WRKY31、Sl WRKY50、Sl WRKY62、Sl WRKY63,运用q RT-PCR分析方法,分析逆境胁迫下表达模式。结果表明,Sl WRKY24干旱、盐、低温胁迫下表达受抑制,另外5个基因在三种胁迫处理中,除干旱胁迫处理Sl WRKY50基因表达量下降,其余均不同程度上调表达。低温胁迫处理下Sl WRKY50基因表达量与对照相比表现极显著差异。Sl WRKY50基因沉默分析结果表明,Sl WRKY50基因沉默后,下调Sl WRKY50基因表达,干旱、高盐胁迫下植株叶片Pro、SOD、MAD含量水平与对照相比变化较小,低温胁迫下番茄植株叶片Pro和SOD含量低于对照,MAD提高含量,植株对低温逆境胁迫耐受能力下降。研究为进一步探讨WRKY基因家族功能提供参考及理论依据。 相似文献
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同型半胱氨酸S-甲基转移酶（homocysteine S-methyltransferase,HMT）普遍存在于被子植物中,在植物合成甲硫氨酸过程中起着重要作用,其在拟南芥中的功能已有所研究,但该家族在其他植物中的功能尚不清楚。本文通过同源比对,获取番茄、拟南芥和葡萄中共8个HMT家族基因的信息,通过系统进化树分析,发现8个HMT蛋白形成2个分支;利用蛋白motif分析、蛋白结构域分析、基因外显子内含子结构分析,发现HMT蛋白在进化上较为保守。通过对拟南芥在非生物胁迫条件下HMT家族基因的表达水平进行分析,发现多个AtHMT基因对非生物胁迫尤其是冷胁迫、盐胁迫、干旱胁迫产生应答,表明HMT家族基因在非生物胁迫应答中的调控作用。另外,通过对番茄各组织在发育的各个阶段中HMT基因进行表达量分析,发现HMT基因可能在番茄种子发育、开花、果实成熟方面发挥作用。综上,本文对番茄、拟南芥和葡萄中HMT基因家族的生物信息学分析及基因表达数据进行了挖掘,对深入研究HMT基因在番茄、拟南芥和葡萄中的作用具有重要意义。 相似文献