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经调查及病理学观察，新疆部分地区土种驴皮肤纤维瘤的发生有地区局限性，公驴多发，以耳下、阴筒、龟头等处多见，呈外生性生长，多见结节状、球状或蕈状，未见肿瘤在不同部位同时生长。瘤体表面有毛或无毛、龟裂、出血，呈肉芽组织样，切面平整灰白，部分呈囊状并有桔黄色液体流出。组织学观察可分单纯性纤维瘤、坏死性纤维瘤、炎性纤维瘤三种类型。 相似文献
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纤维瘤是来源于纤维组织的一种良性肿瘤,依据瘤体中纤维和细胞成份的比例不同,分为硬性纤维瘤和软性纤维瘤两种.前者多见于皮肤、粘膜、肌膜、骨膜、腱;后者多发于皮肤粘膜、浆膜等部位.纤维瘤常单个发生,一般对机体影响不大,但瘤体过大,则会压迫周围的血管神经,对机体造成不良影响.兽医临床上多采用手术方法进行摘除. 相似文献
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正纤维瘤是由结缔组织增生导致的良性肿瘤,包括幼稚型纤维细胞及多时胶原等结构,常见于多种家畜。只要含有纤维性结缔组织的位置,均有概率出现纤维瘤。仅体现结缔组织性的纤维瘤被称作真性纤维瘤,多发于家畜的四肢皮肤、头部、腹部或胸部,可通过手术治疗使家畜恢复健康。下面,笔者就犬颌骨部纤维瘤的诊断、手术要点以及诊疗实例加以介绍。 相似文献
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纤维瘤是来源于纤维组织的一种良性瘸,可发生于动物全身各个部位,但以皮肤及皮下组织最为常见,口腔卵巢均可发生,根据纤维瘤内所含纤维及细胞成分的比例不同,可分为硬性纤维瘤和软性纤维瘤。临床上多 相似文献
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纤维瘤是牛及其他家畜常见的良性肿瘤之一,身体的各个部位均可发生。皮肤外伤可引起皮肤纤维瘤的发病。动物的表层皮肤发生创伤时,要及时处理,可减少皮肤纤维瘤的发生。 相似文献
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不同饲喂模式对奶牛采食和瘤胃发酵的影响 总被引：3，自引：0，他引：3
为采用4头体重(600±50)kg、装有永久性瘤胃瘘管的中国荷斯坦经产奶牛进行试验,比较传统精粗分开饲喂向全混合日粮(TMR)转换时泌乳奶牛的采食、瘤胃发酵及生产性能的变化。试验动物所用日粮组成在整个试验期完全相同。前17 d采用精粗饲料分开饲喂的模式,后13 d则采用TMR进行饲喂。在由传统的精粗分开饲喂向TMR饲喂转换过程中,试验牛的日粮干物质采食量(DMI)、中型洗涤纤维进食量(NDFI)差异极显著(P<0.01),而与之相对应的采食时间差异不显著;全天的瘤胃pH值试验期前后比较无明显差异,但pH曲线但有趋于平稳的趋势,瘤胃中氨态氮浓度在试验期结束时差异极显著(P<0.01)。 相似文献
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牛病毒性腹泻-粘膜病病毒TaqMan荧光定量RT-PCR检测方法的建立及初步应用 总被引：1，自引：0，他引：1
为建立-种牛病毒性腹泻-粘膜病病毒（BVDV）的快速检测方法,根据GenBank中登录的BVDV基因序列,针对5’UTR的保守序列,设计合成了一对特异性引物和一条TaqMan荧光探针。通过对反应条件和反应体系进行优化,建立了一种能快速定量检测BVDV的荧光定量RT—PCR检测方法。通过对20份胎牛血清样品和猪瘟细胞苗半成品进行检测,对该方法的特异性、敏感性和重复性进行试验。结果显示,建立的方法能检测到BVDV,而对CSFV、PRRSV和MDBK细胞的扩增结果均为阴性,具有高度的特异性。在所检测的20份样品中,BVDV阳性6份（30％）。对所构建的标准品进行检测,在10^2-10^8拷贝／μL的范围内可以得到良好的动力学曲线,能检测低至10^3-10^4拷贝／mL的病毒量。表明所建立的检测方法具有快速、特异、灵敏、重复性好等特点,可用于临床及科研中对BVDV的快速定量检测和对牛血清及猪瘟兔化弱毒疫苗等生物制品中是否污染BVDV进行监测。 相似文献
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旨在筛选适合生物反应器悬浮培养BHK21细胞的无血清培养基,并利用该技术培养伪狂犬病毒。通过筛选市售的无血清培养基,以细胞形态、细胞活率和增殖倍数作为驯化指标,对BHK21细胞进行无血清驯化;对使用10 L和40 L生物反应器无血清悬浮培养BHK21细胞的参数进行研究;同时利用筛选的无血清培养基和悬浮细胞进行伪狂犬病毒增殖测试。结果表明,市售培养基Ⅰ符合筛选要求,其培养的细胞形态比较均一,结团少,细胞生长2 d的增殖倍数为3.5倍,细胞活率在95%以上;生物反应器悬浮培养BHK21细胞的最适参数为转速80 r/min、pH值7.2、DO 60%;利用该工艺技术培养伪狂犬病毒,当接种细胞密度为6×106 cells/mL,接毒剂量为0.3%时,48 h收获的病毒液滴度可达109.5 TCID50/mL。该试验实现了伪狂犬病毒的无血清悬浮生产,为其生产工艺的优化提供了可能。 相似文献
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Laurent Blond Donald E Thrall Simon C Roe Nadege Chailleux Ian D Robertson 《Veterinary radiology & ultrasound》2008,49(5):425-431
A stifle magnetic resonance (MR) imaging protocol was developed based on the appearance of the cruciate ligaments and menisci in normal dogs. Proton density images were subjectively considered to have the highest likelihood of detecting a meniscal lesion. Following this initial evaluation, the accuracy of high-field MR imaging to detect meniscal tears in dogs was evaluated in 11 dogs suffering from naturally occurring cranial cruciate ligament rupture. Dogs underwent MR imaging of the affected stifle before surgery. MR imaging and surgical findings were assessed independently, and then compared. Five tears of the medial meniscus were correctly diagnosed with MR imaging and 19 normal menisci were accurately characterized as such, based on MR images. In one medial meniscus, changes consistent with meniscal degeneration were seen on MR images but this was not seen at surgery. With regard to the lateral meniscus, one false positive diagnosis of a tear was made and this likely represented a normal variation. One other lateral meniscus had changes consistent with meniscal degeneration but, as with the similar lesion seen in the medial meniscus, this was not confirmed surgically. The global sensitivity of MR imaging for the diagnosis of a meniscal tear was 100% and the specificity was 94%. High-field MR imaging is a reliable method to diagnose meniscal tears preoperatively and this may be useful in selecting the surgical approach to clinically abnormal joints and may decrease the need for arthrotomy. 相似文献
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旨在制备猪RNA聚合酶Ⅱ的单克隆抗体并进行初步应用。本研究采用生物信息方法预测免疫原，将其化学合成后免疫5只4~8周龄Bal b/c雌性小鼠，对免疫后呈现阳性的小鼠进行细胞融合试验，取其脾细胞与骨髓瘤细胞进行融合并获得能稳定分泌抗RNA PolⅡ的杂交瘤细胞。鉴定结果显示，RNA PolⅡ单克隆抗体的重链为IgG 2A型，轻链为Kappa型。利用间接ELISA方法对杂交瘤细胞进行筛选和亚克隆，获得了8株稳定分泌RNA PolⅡ单克隆抗体的杂交瘤细胞株。将其初步应用到染色质免疫共沉淀（ChIP-seq）技术，并与商业化抗体的富集性进行比较，结果表明，本研究得到的单克隆抗体富集性更强。本研究获得的猪RNA PolⅡ单克隆抗体可为表观遗传学的研究提供理论基础，并且提供重要的生物学材料。 相似文献
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用纯化的猪伪狂犬病病毒gB重组蛋白为抗原,建立了检测猪伪狂犬病血清抗体的gB-ELISA方法。最佳反应条件为：抗原包被浓度为3.15μg/mL,待检血清稀释度为1∶40。该方法对猪圆环病毒病、猪瘟、猪细小病毒病、猪繁殖与呼吸综合征（猪蓝耳病）、猪乙型脑炎、猪布氏杆菌病5种疾病阳性血清和SPF猪阴性血清检测呈阴性反应。批间、批内试验变异系数均不超过8%。用该方法与HerdChek ELISA试剂盒同时对119份血清进行了平行检测,其相对敏感性、特异性和符合率分别为：75%、80.7%和79%。试验结果表明：猪伪狂犬病血清抗体gB-ELISA检测方法具有较高的敏感性和特异性,且重复性好,可用于猪伪狂犬病毒血清抗体检测。 相似文献
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减少瘤胃甲烷排放措施的研究已成为当前的研究热点之一。本研究以酒、面包和畜禽专用酿酒酵母产品为原材料,从中分离到三株酵母菌,将其制成冻干粉,通过体外产气试验,筛选出了一株抑制瘤胃甲烷排放效果最为显著的酵母菌株。然后以该酵母菌株作为研究对象,进行分子生物学鉴定;采用分光光度计比浊法测定该酵母菌株的生长曲线,确定菌体生长规律;并以此为依据,设计单因素试验考察该酵母菌株最适宜的生长条件。结果表明,分离得到的酵母菌株均为酿酒酵母saccharomyces cerevisiae;抑制瘤胃体外甲烷排放效果最显著的1株酵母菌分离自面包中;该酿酒酵母菌株的对数生长期为8～24h,其最佳培养条件为:生长温度30℃,pH 5.5,培养基中葡萄糖的浓度4.0%。 相似文献
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The objective of this research was to develop a prototype system for national cattle evaluation that would facilitate selection for improved fertility of daughters from Hereford sires. Raw data for this analysis were the birth dates of calves as reported by breeders to the American Hereford Association. Records from females entered this analysis with the reporting of a birth date for their first calf. At that time, females were required to be in contemporary groups of at least 3 animals and to have at least 2 additional paternal half-sibs also represented in the data. To explicitly define "sustained reproductive success," the philosophy taken was that a female that maintained a calving interval of 425 d or less would be considered successful. Females failing to meet this criterion were considered to be at the end of their successful lifetime. Data were analyzed using methodology for survival analysis with grouped data. Fixed contemporary groups were modeled as being time dependent, reflecting the females exposed for breeding in the same herd-year-season. Sire effects were time independent and considered random. Also included in the analysis were time-independent covariates for maternal weaning weight and total maternal calving ease from the national cattle evaluation of the American Hereford Association. Records from females still successfully in production at the time of this analysis, those that were transferred, those with calving intervals less than 280 d, and those that were successful until becoming donor dams for embryo transfer were considered censored. A total of 36,866 females contributed to this analysis, with 14,143 of these having censored records. The median number of females in a contemporary group was 6. A total of 3,323 sires had daughters with records. The median number of daughters per sire was 7. Heritability of sustained reproductive success on the underlying scale estimated from these data was approximately 0.05. Additional data accumulated over time will improve this genetic evaluation. Sustained reproductive success is important to the commercial beef industry, and results from this evaluation are expected to enhance the assessment of economic value of Hereford seedstock. 相似文献
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褪黑激素（MLT）是调控动物季节性生殖等多种生物节律,具有复杂生理功能的一种吲哚类激素,羟基吲哚-氧-甲基转移酶（hydroxyindole-O-methyltransferase,HIOMT）是决定MLT合成波动模式的重要酶之一。采用RT-PCR法克隆并分析了牦牛、犏牛和藏黄牛HIOMT基因的CDS序列。牦牛该基因序列连续缺失123bp,通过与普通牛基因组序列比对,该缺失片断位于HIOMT基因7个外显子中的第6外显子。由于克隆所得牦牛基因组DNA相应序列未缺失上述片断,推定所获得的牦牛HIOMT基因CDS序列属可变剪接体;克隆犏牛HIOMT基因获得长、短两条件序列,长序列与藏黄牛、普通牛该序列相似,短序列与牦牛相似,但尚不能确定这两条序列各自来自父本还是母本;与普通牛HIOMT基因比较,牦牛、犏牛和藏黄牛分别有11、13（长序列17）和11个变异位点,且位于密码子第1、2位点的变异分别为4、6（7）和4个,均为非同义突变,所有非同义突变只有1处由密码子第3位变异引起,这些突变可能通过影响蛋白结构进而影响其生物学功能。还预测了牦牛、犏牛、藏黄牛和普通牛HIOMT蛋白相对分子量、理论等电点、疏水性、跨膜区等。HIOMT的保守性较强,是系统进化研究较理想的分子标记。 相似文献
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利用PCR-RFLP技术对贵州矮马、德保矮马和宁强矮马3个品种的45个个体的肌肉生长抑制素(myostatin, MSTN)基因的启动子区进行多态性分析。结果表明,大小为204 bp的扩增片段经限制性内切酶SspⅠ酶切后,不同基因型经过测序发现156T→C突变,产生AA和AB两种基因型,没有发现BB基因型。贵州矮马和宁强矮马中AB基因型频率大于AA基因型,德保矮马中AA基因型占优势,在3种矮马品种A等位基因频率均大于B等位基因。卡方检测结果发现,贵州和德保矮马在该酶切位点处于平衡状态(P＞0.05),而宁强矮马在该位点分布差异显著(P＜0.05)。 相似文献