2. The yolk: albumen ratio of the goose egg was higher.
3. The fat concentration in the egg yolk and the protein concentration in the egg albumen were lower.
4. Deposition of dry matter in the embryo and energy expenditure during incubation were similar. In both species, the nitrogen in the embryo exceeded the nitrogen in the egg contents. This is probably due to the utilisation of egg‐shell membrane proteins.
5. Lysine concentration in the goose egg proteins was lower, which was reflected in the hatched gosling proteins.
6. There were high correlations in amino acid concentrations between chicken and goose eggs and between the efficiencies of amino acid utilisation by their embryos, implying that similar metabolic processes are involved in these two species. 相似文献
2. Resonances from adenosine triphosphate, phosphocreatine, inorganic phosphate and phosphorous storage protein were observed. These changed in relative intensity as the embryo developed.
3. No ill effects due to exposure to radiofrequency radiation or magnetic fields were observed.
4. It is concluded that topical magnetic resonance can make a useful contribution to the study of embryonic development in avian eggs. 相似文献
2. The decrease in hatchability that occurred on the high protein (27.0 g CP), low energy (1.52 MJ AME) allowance from 26 to 36 weeks of age was due to an increase in the percentage of dead embryos in the second week of incubation and an increase in the number of “pipped” eggs at the end of incubation.
3. The low hatchability of eggs from birds on the 1.88 MJ AME allowance from 37 to 64 weeks could be related to the incidence of deaths in the first 5 d of the incubation period.
4. Malformations and malpositions of the embryo were not affected by maternal energy or protein allowance. 相似文献
For all minerals the relationships between the amounts present in the embryo and the embryo dry weight were allometric over this period.
There were significant, positive, within‐day correlations between the amounts of copper, manganese, sodium and potassium and between iron, magnesium and zinc present in the embryo.
Only in the cases of iron and sodium was there evidence to suggest that the total amount of the element present in the egg was correlated with the amount transferred to the embryo. 相似文献
2. Rocking embryo cultures during the first half of incubation enhanced embryo growth.
3. Embryos explanted into ‘surrogate’ eggshells of either other individuals or other species have been successfully ‘hatched’.
4. A normal chorioallantois is formed in these surrogate eggshells. This enables a functional albumen sac to form and eggshell resorption to be achieved.
5. Embryos grown in ‘surrogate’ eggshells are slightly smaller than controls but otherwise normal.
6. The technique provides opportunities for genetic engineering experiments. 相似文献
2. Growth of both embryo and wing length during 42 d of incubation was comparable and approximately linear, with a more or less weekly doubling in size up to 35 d of incubation.
3. The embryo eye size increased more rapidly than beak length and reached a maximum of ~16.2 mm by 28 d of incubation, whereas beak length increased continuously until hatching at 42 d.
4. Linear regression equations were derived from morphometric measurements of embryos between 7 and 42 d.
5. Information stemming from these results can be used to estimate the age of dead-in-shell embryos in an attempt to identify timing of incubation problems that potentially result in low hatchability of fertile eggs. 相似文献
2. A simple technique for chicken manipulation is eggshell windowing, without or with seal, the latter having demonstrated some improvement in hatching rates.
3. Likewise, a surrogate eggshell system provides an accessible model for manipulation during chicken and quail development, with a higher hatchability compared to the simple windowing method.
4. The development of the chicken ex ovo culture systems in a synthetic environment as an efficient technique for imaging and microsurgery applications has enabled the study of important events of live chicken embryos at a specific time point.
5. This short review illustrates recent applications of well-designed whole embryo culture systems as a robust model for research into numerous biological mechanism, drug discovery, gene manipulating and production of functional proteins. 相似文献
2. Liver fat, carcass fat, liver weight and body weight increased markedly at the onset of sexual maturity.
3. Within the liver, the protein to DNA ratio did not alter significantly during development; glycogen was not consistently depleted by egg production.
4. Liver fat content was significantly correlated with carcass fat, liver weight and DNA concentration, and plasma total lipid and cholesterol, independent of stage of development; with body weight, liver protein, moisture, and protein to DNA ratio, excluding the period prior to commencement of sexual maturation; and with plasma phospholipid during lay.
5. Obesity was a feature of faster growing fowls which matured earlier, consumed more, utilised food less efficiently for egg production and produced fewer saleable eggs. 相似文献
2. The low AME was due to a reduced starch digestibility and was not improved by grinding the wheat finely or by steam pelleting the diet.
3. Higher AME values were obtained when determinations were made with adult cockerels than with growing broilers when the conventional technique was used.
4. AME values determined using a rapid bioassay technique (Farrell, 1978) with growing broilers or adult cockerels were considerably higher than values determined using the conventional procedure with growing broilers.
5. Inter‐laboratory studies showed that the true metabolisable energy (TME) assay gave reproducible values for individual and blended wheat samples, but that these values were higher than AME values determined by the conventional procedure. Similar TME values were found with growing broilers and adult cockerels. 相似文献
2. The proportion of available iodide transferred into the oocytes appeared to be more dependent on the amount of iodide injected and the total weight of growing oocytes than on a requirement for either a store of iodide for the embryo or an iodide excretory pathway for the hen.
3. The thyroid was about four times more active in accumulating iodide than the oocyte.
4. The percentage of iodide accumulated by the plasma was the same at all dose rates. 相似文献
2. The quail were kept in open‐circuit respiration calorimeters, so that diet selection could be related to energy requirement.
3. Increasing ambient temperature had no significant effect on food intake by weight, but the proportion of the high energy choice decreased and, conversely, the proportion of the lower‐energy but higher‐protein choice increased.
4. Energy intake was therefore negatively correlated with ambient temperature, but protein intake per unit of energy intake increased, allowing the birds to gain weight at about the same rate at all temperatures.
5. Heat production decreased as ambient temperature increased. Respiratory quotient decreased with increasing temperature, which indicates a reduced utilisation of carbohydrate as an energy source.
6. Water intake increased with temperature but there were no overt signs of heat stress and there was no significant change in body temperature.
7. Japanese quail selected a dietary mixture which maintained similar growth rates over a wide range of ambient temperature, by sustaining protein intake but altering energy intake in line with thermoregulatory energy demands. 相似文献
2. A combination of 3 growing climates, 3 laying climates (temperate, hot dry, hot humid), and 2 dietary energy concentrations (10.03 and 11.70 MJ ME/kg with protein concentrations proportional to energy) were tested for 46 weeks using 432 point‐of‐lay pullets.
3. Both growing and laying climates significantly affected most traits measured during the laying period. The patterns of egg production showed good persistency in all environments and differences observed reduced with age.
4. The difference between the high and low energy intake reduced at high ambient temperatures. Feeding low energy diets did not affect mean age at first egg, rate of lay or the egg output in the hot dry environment, nor egg weight in either hot dry or hot humid climates. Rather, feeding low energy diets resulted in improved body weight change in all 3 laying environments.
5. The growing climate and diet interacted significandy on body weight change, while highly significant interactions between laying climate and diet occurred on rate of lay, food and energy intake and egg weight during the laying period. 相似文献
2. Food intake was measured daily for 21 d. Body composition was determined at 42 d and gains in body mass, protein, fat and gross energy calculated by comparison with a group analysed at 21 d. Heat production was calculated by difference between AME intake and energy gain.
3. Decrease of food mass intake with increased dietary AME concentration limited the increase in AME intake to about 25%, despite the near 2‐fold range of AME concentrations.
4. There was no effect of CP concentration on food mass intake. CP intake was directly related to CP: AME ratio.
5. When body weight differences were taken into account, heat production was independent of dietary AME concentration, but increased by about 8% on the higher‐protein diets.
6. There were strong linear correlations between dietary CP:AME ratio and carcase protein: energy ratio, carcase fat content and carcase protein content.
7. It was concluded that the growing fowl responded to dietary nutrient: energy ratio, and the associated differences in nutrient and energy intakes, by varying the rate of energy deposition as fat, without regulatory variation of energy dissipation as heat. 相似文献
2. Although the protein content of all tissues varied with age, the only statistically significant effect was on the level of protein in the gizzard.
3. The moisture content of the gizzard was significantly affected by age and blood by age and sex.
4. The fat content of the gizzard was significantly affected by age, as was the effect of sex on the fat content of blood.
5. The ash contents of heart and gizzard were significantly affected by age.
6. The phosphorus contents of the gizzard, heart and liver were significantly affected by age, as were the chloride content of heart and the calcium content of the liver. Blood potassium was significantly affected by sex. Fluctuations in the contents of other elements are discussed.
7. The variability in organ composition is discussed with respect to the degree of trimming and preparation of the samples. 相似文献
2. In both lines, the increase of egg storage temperature from 12°C (standard level) to 30°C increased the egg weight loss during storage, shortened the incubation period and reduced the hatching success.
3. Similar to standard egg storage temperature, LG quail hatched earlier than HG quail after egg storage at 30°C and early and late mortality of both lines increased with a prolonged period of egg storage. In contrast to the standard egg storage conditions, no line differences in hatchability were observed.
4. The results did not identify a relationship between the decrease in hatchability or embryo viability and line differences in external egg parameters as well as any important role of undesirable changes induced by a high storage temperature on albumen viscosity.
5. The pattern of embryonic death, low developmental rate of embryos and a dichotomy between the development of the extra-embryonic vascular system and the embryo itself during egg storage at high temperature implied that an insufficient nutrient supply in consequence of developmental delay could represent a key factor in increasing early and late embryo mortality. 相似文献
2. In this experiment, carcase analyses of each of three breeds of pullets were conducted at weekly intervals throughout the growth of the pullets, to 18 weeks of age. Measurements were made of body weight, gut‐fill and feather weight, and chemical analyses consisted of water, protein, lipid and ash measurements of both the body and the feathers. Each age group comprised 10 birds of each breed.
3. Gompertz functions accurately estimated the growth of both body protein and feather protein, to 18 weeks of age, from which the rate of growth of these two components of the body could be estimated. The mature weight of pullets was overestimated by the Gompertz growth curve, which may indicate that a pullet ceases to increase in body protein content once sexual maturity has been reached.
4. Using allometric relationships between the chemical components of the body and of feathers, all the components of growth could be estimated from the growth of body protein and feather protein. These components were then added together to determine the growth rate of the body as a whole.
5. The daily amino acid requirements for 4 functions were calculated, namely, those for the maintenance of body protein and feather protein, and for the gain in body protein and feather protein. These requirements were then summed to determine the requirement of pullets on each day of the growing period.
6. Using the ‘effective energy’ system, the amount of energy required by these pullets was calculated for each day of the growing period, from which the desired daily food intake of the pullets could be predicted. By dividing the amino acid requirement by this daily food intake it was possible to determine the concentration of amino acids that would be needed in the diet in order to meet the requirements of a pullet.
7. The results indicate that the ratio between the requirement for lysine and for methionine and cysteine changes dramatically during the growing period, negating the concept of a fixed ratio between all the amino acids during growth.
8. The above process is the first step in determining the optimal feeding programme for a population of pullets of a given genotype. The constraining effects, of the diet being offered and of the environment in which the pullets are housed, on the food intake and growth rate of each pullet have to be estimated, and such a theory can then be expanded to include all the individuals in the population. Only by the use: of simulation models can all these constraining effects be considered simultaneously. 相似文献
Only the growing ration containing 1.83 per cent magnesium significantly reduced growth, percentage bone ash and food utilisation during the growing phase (9–20 weeks). In the laying phase (20–40 weeks) significantly greater gains in body weight and percentage bone ash were produced by the birds which had received the growing ration containing 1.83 per cent magnesium.
The magnesium content of the growing rations had no effect on rate of lay, egg weight or egg shell thickness.
Hens fed the laying ration containing 1.96 per cent magnesium gained less body weight, laid fewer and lighter eggs and utilised their food less efficiently than those fed other levels of magnesium.
Egg production and food required per dozen eggs were poorer with the ration containing 1.20 per cent magnesium, but were not statistically different from the rations containing 0.48 or 0.70 per cent magnesium.
Eggs with thinner shells were produced by hens fed the 1.96 per cent magnesium ration at 34 weeks of age than were produced by those fed the 0.48 or 0.70 per cent magnesium rations. 相似文献
2. Body‐weight gain and carcass fat and water content increased and fertility decreased with increasing energy allowance. Maximum egg production occurred at an energy intake of 1.73 MJ AME/bird d.
3. Differences in egg weight and hatchability were related to differences in both energy and protein intake. The highest egg weight occurred at the highest allowance of energy and protein. Hatchability was depressed where the daily allowances of protein and energy were in a ratio of more than 15 g protein: 1 MJ AME.
4. Apart from egg size no significant effects on reproductive performance were observed when dietary protein intake was varied from 27 to 19.5 g/bird d.
5. Requirements of broiler breeder hens for protein during lay may be lower than previously thought. For the strain used a protein intake of 19.5 g/bird d appeared adequate provided essential amino acid concentrations were maintained.
6. The close relationships between body weight and energy allowance and the latter and egg production make body‐weight gain a useful guide to management. A body‐weight gain of about 1.1 kg from 21 to 36 weeks of age was associated with optimum performance in this study. 相似文献
2. Fresh laid eggs were stored at 15°C for 3, 7, or 14 d (group 1) or held initially at room temperature (21°C) for 6 to 9 h and subsequently stored at 15°C for 3, 7, or 14 d (group 2).
3. Most embryos in eggs from both groups progressed developmentally to Stage‐8, just prior to onset of hypoblast formation. There were no significant differences in embryo development either within or between groups.
4. It is concluded that under the conditions of this study, egg cooling and storage augment development of the embryo, albeit only slightly. 相似文献
2. The values for fractional synthesis rate (FSR) estimated in vitro at 20, 30, 45 and 60 min during the embryo culture agreed well, ranging from 35 to 40%/d, suggesting that the method would serve as a useful model for studying the effect of growth promoters in chick embryos.
3. Bovine insulin in the synthetic medium did not affect FSR of protein in chick embryos cultured in vitro. 相似文献