共查询到19条相似文献，搜索用时 445 毫秒
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用三种不同水质配制培养基对马铃薯脱毒试管苗的影响 总被引：14，自引：3，他引：11
1 前 言马铃薯茎尖脱毒试管苗扩繁培养是一项投资大、成本高的工作。在脱毒种薯的生产中 ,我地区主要以扦插移栽为主 ,这样需要数以万株的健康茁壮的试管苗。以往我们采用蒸馏水做培养基 ,这样不但用量大 ,也浪费水。本着降低生产成本 ,又能生产壮苗的目的 ,我们进行了用雨水 (雪水 )、自来水 (开水 )、蒸馏水配制不同培养基对试管苗影响的试验 ,以蒸馏水为对照。通过观察与比较 ,找到了用雨水配制培养基最有利于试管苗生长的根据。2 材料与方法( 1)试验材料 :内薯 7号。( 2 )培养条件 :培养温度白天为 2 1℃ ,夜间为 16℃ ,光照强度 2 0… 相似文献
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马铃薯脱毒试管苗在防虫温室生产一季微型薯,生产期间通过加强肥水管理和病虫害防治获得健壮的苗,收获微型薯后,再进行大田移栽,测定其二次结薯情况。结果表明,抗疫白产量为26808kg·hm-2,润者为19715kg·hm-2。其产品可作为原种一代,既解决了马铃薯脱毒试管苗的综合利用问题,又缩短了育种年限。 相似文献
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1 前 言目前 ,马铃薯脱毒技术已经很广泛地应用于马铃薯生产 ,并取得了非常明显的增产、增收的效果。但常规利用试管苗扦插生产微型薯的技术存在成本高 ,生产出的微型薯百粒重低 ,在进一步繁育过程中会导致发芽势弱 ,容易烂薯的现象。同时 ,试管苗扦插生产出的微型薯中小于 1ｇ的薯较多 ,这些非常小的微型薯就更难在生产上直接应用。为了降低成本 ,提高微型薯百粒重 ,我们于 2 0 0 1年春季进行了试管苗扦插与微型薯苗扦插生产微型薯技术的比较试验。2 材料与方法2 1 材料供试材料为郑薯 5号脱毒微型薯 (小于 1ｇ)、脱毒试管苗 ,郑薯 6… 相似文献
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马铃薯试管薯生产技术规程 总被引：2，自引：0，他引：2
西吉县马铃薯生产研究所自1998年创建以来，一直通过试管苗移栽成基础苗，然后在基础苗上剪尖扦插的方法生产原原种，在多年的生产过程中出现两个缺点，一是试管苗移栽基础苗时成活率不高，二是在剪尖扦插的过程中脱毒苗易被染病。因此，在2003年我们设想用试管薯代替基础苗生产原原种，并尝试性进行生产试管薯，结果获得了试管薯50万粒，2004年扩大生产量收获试管薯300万粒，2005年1～3月份又成功收获试管薯100万粒。现将我们探究和总结出的试管薯生产技术介绍如下，以供马铃薯脱毒研究和生产者参考应用。 相似文献
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不同培养方式和成分对马铃薯脱毒试管苗生长的影响 总被引：10，自引：0，他引：10
1 前 言组织培养马铃薯植株 ,并进行微繁和脱毒种薯的生产 ,在实践中已得到广泛应用。甘肃省农科院脱毒中心每年生产 10 0万粒原原种 ,需要提供大批量的脱毒试管苗。为了降低成本 ,适合产业化生产的需求 ,我们对试管苗的培养基进行了研究 ,从培养方式、碳源和用水三方面进行了一系列对比试验。通过观察比较 ,找到了马铃薯试管苗较好的培养方式和优化搭配 ,提出了一套脱毒试管苗快速繁殖及优质、低成本的生产方案。2 材料与方法2 1 供试材料本中心提供的陇薯 3号脱毒试管苗。 收稿日期 :1999-0 8-152 2 方法以ＭＳ为基本培养基 ,… 相似文献
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生根粉剂诱导马铃薯脱毒试管苗生根研究 总被引：2，自引：1，他引：1
1 前 言在马铃薯脱毒试管苗生根培养中 ,通常添加ＢＡ做生根诱导 ,在生产中 ,为了缩短培养时间 ,降低生产成本 ,我们使用生根粉 (四川省兰月科技开发公司生产 )代替ＢＡ诱导试管苗生根。本试验的目的是比较添加生根粉剂和ＢＡ不同处理对马铃薯试管苗生根、生长及结薯情况的影响 ,探讨使用生根粉代替ＢＡ诱导试管苗生根的可行性。2 材料与方法2 1 试验材料津引薯 8号脱毒试管苗。2 2 试验方法2 2 1 生根诱导培养基处理 :使用ＭＳ液体培养基 ,10 0 0ｍｌ加入活性炭 2ｇ ,蔗糖 30ｇ ,ｐＨ值 5 8,做两个对照处理 :①ＭＳ 生根粉… 相似文献
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对3种不同苗源（试管薯、试管苗、扦插苗）生产脱毒马铃薯原原种的产量进行对比试验,结果表明：（1）在常规栽种季节（四川地区春季为3月20日~4月20日,秋季为8月25日~9月25日）的条件下,试管薯生产原原种的产量最高,每株平均为3.5粒,试管苗与扦插苗产量相当,每株平均为2.8粒;（2）在延迟栽种的情况下,其产量高低为试管薯〉试管苗〉扦插苗,试管薯每株平均为1.9粒,试管苗为2.0粒,扦插苗为1.8粒;（3）方差分析表明,常规栽种季节试管薯与试管苗和扦插苗的产量分别达显著水平,延迟栽种条件下3者产量均未达显著水平。因此,大规模生产脱毒马铃薯原原种企业在常规栽种季节建议使用试管薯苗源,而延迟栽种的情况下建议大量使用扦插苗进行生产。 相似文献
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Abdus Sattar Neelofar M. A. Akhtar 《Plant foods for human nutrition (Dordrecht, Netherlands)》1990,40(3):185-194
Influence of irradiation (0.05–0.20 kGy) and germination (24–120 hours) in distilled and tap water on phytate, protein and amino acids of soybean, was studied. Phytate values significantly decreased with increasing germination period and irradiation dose (P<0.01). Irradiation independently decreased the original phytate (212.0 mg/100 g) to a range value of 205.0–190.0 mg/100 g depending upon dose level. Germination of unirradiated seeds for 120 hours in distilled and tap water lowered the phytate to 55.0 and 94.9 mg/100 g (74.1 and 55.2% reduction) respectively. Maximum destruction of phytate to levels of 20.5 and 50.9 mg/100 g (90.3 and 76.0% reduction) occurred during germination of 0.20 kGy samples for 120 hours in distilled and tap water respectively. Total protein content significantly increased during germination (P<0.05) and the increase was more in tap than distilled water. Germination for 120 hours of untreated seeds in tap water increased the essential and decreased non-essential amino acids while in the 0.10 kGy sample, increases in both cases were observed. 相似文献
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Summary Experiments were conducted to evaluate the efficacy of two triazoles, triadimefon and uniconazole, compared to benzylaminopurine
(BAP) for in vitro microtuber production in three leading Indian cultivars, Kufri Jyoti, Kufri Badshah and Kufri Sindhuri.
Both triazoles were effective in microtuber production, and at the lowest concentration tested (0.01 mg 1−1) uniconazole was more effective in producing higher numbers and average weight of microtubers than 10 mg 1−1 of BAP, although the best results were obtained with 0.5 mg 1−1 of uniconazole. This is the first report of triazole-induced stimulation of in vitro microtuber production. 相似文献
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苗龄及光照对诱导马铃薯微型薯的影响 总被引：3，自引：1，他引：3
研究不同周龄的试管苗在诱导后期施加不同光照处理对马铃薯品种东农303微型薯诱导的影响。结果表明,随着苗龄的增长,诱导出的微型薯数量和重量均呈双峰抛物线型;光强增大可促进薯块的增大、薯重的增加和大薯率的提高,但对结薯数量的影响不大。 相似文献
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《Field Crops Research》2006,95(1):89-96
Tuber yields of potato plants grown from microtubers in fields are more variable than yields from conventional seed tubers (CT). One reason could be their higher susceptibility to water stress. This study clarified the effect of soil water stress from 1 month after emergence on the growth and yield of plants grown from conventional seed tubers and microtubers in fields. Microtubers (0.5–3 g) and conventional seed tubers (50 g) were grown in Hokkaido, Japan, over three field seasons. One month after emergence, poly-shelters were placed over the plots to prevent rainfall, and either irrigated (wet plot) or non-irrigated (dry plot) treatments were formed. At mid-flowering (about 50 days after emergence) leaf area index (LAI) in microtuber plants was decreased relatively more due to soil water stress than LAI in conventional seed tuber plants. However, at maximum shoot growth (about 80 days after emergence) both microtuber and conventional seed tuber plants had a similar relative decrease in LAI due to soil water stress. At mid-flowering and maximum shoot growth microtuber and conventional seed tuber plants had reduced stomatal conductance due to soil water stress, but the reduction in stomatal conductance was greater in conventional seed tuber plants than in microtuber plants. Microtuber and conventional seed tuber plants had similar root development at maximum shoot growth. Tuber production from mid-flowering until plant maturity was similarly affected by soil water stress in microtuber and conventional seed tuber plants. At harvest, plants affected by soil water stress had about 87% of the tuber dry weight of irrigated plants. We conclude, that the greater variation on tuber yield of microtuber plants cannot be attributed to soil water stress from 1 month after emergence. 相似文献
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Effects of Age and Pretreatment of Tissue-Cultured Potato Plants on Subsequent Minituber Production 总被引：1，自引：0，他引：1
Mirko Milinkovic Corina B. Horstra Brendan C. Rodoni Marc E. Nicolas 《Potato Research》2012,55(1):15-25
Potato (Solanum tuberosum L.) plantlets were conditioned in tissue culture to produce more minitubers when planted in a greenhouse. Tissue-cultured
potato plantlets, varieties Coliban and Russet Burbank, were grown for 4, 10, or 12 weeks under three temperature regimes
(22/18 °C, 16/10 °C, and 10/2 °C day/night) and a photoperiod of 16, 12, or 8 h in different stages of growth. Duration, temperature,
and photoperiod of the in vitro period affected plantlet morphology and the total number of minitubers produced per plant
in a greenhouse. Extending the growing period and introducing a hardening period with low temperatures (16/10 °C) during the
in vitro production stage resulted in 97% more minitubers (4.94 vs 2.50 minitubers per plant for the control) in variety Coliban
and up to 71% (6.50 vs 3.80 minitubers per plant for the control) in variety Russet Burbank. The total number of minitubers
produced per plant did not change significantly for both varieties when a shorter photoperiod was used instead of the standard
16-h day during the growing period in vitro. Results presented in this experiment demonstrate that the number of minitubers
can be substantially increased through the introduction of an induction period as an interstage between the in vitro stages
of potato plantlet production and minituber production. 相似文献
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Summary CIRAD developed a new apparatus for plant tissue culture, using temporary immersion in a liquid medium. This apparatus was
adapted to the microtuber production in potato. The procedure is as follows: single node cultivation on MS medium containing
30 g/l sucrose in the light for 2 weeks, induction of microtuberisation with 80 g/l sucrose over a 2 week period in the light,
followed by a further 6 weeks in the dark. All experiments were performed at 20 °C. The basic vessel had a capacity of approximately
11;30 nodes were cultivated per vessel. Depending on the cultivars tested (Bintje, Ostara and Désirée) 47 to 115 microtubers
were harvested per vessel. Between 30 and 60% of the microtubers weighted over 0.5 g and between 10 and 40% over 0.8 g. Sprouting
is still under investigation. Preliminary results indicate that the dormancy period was relatively short and several stems
were obtained per microtuber. These results seem to be better than those usually reported. Only one simple protocol has been
tested and further improvements are probably easy to obtain. 相似文献