共查询到20条相似文献，搜索用时 437 毫秒
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8-羟基喹啉和柠檬酸对切花菊生理效应的影响 总被引：3，自引：0，他引：3
以不同浓度8-羟基喹啉(8-HQ)和柠檬酸(CA)进行组合作为保鲜液,通过外部形态观察和生理指标测定,研究保鲜剂对切花菊的保鲜效果。结果表明:8-HQ和CA对延缓切花菊的衰老有明显作用,可延长其瓶插寿命,增加花枝鲜重,减缓花瓣组织中蛋白质和糖含量的分解速度。处理A 3%蔗糖+100 mg/L 8-HQ+200 mg/L CA保鲜效果最好,可使切花菊的瓶插寿命比对照延长10 d。 相似文献
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不同保鲜液对龙船花切花的保鲜效果 总被引：4，自引：1，他引：3
以龙船花（Ixora chinensis）切花为试材,通过测定瓶插寿命、鲜样质量、花瓣质膜透性和花瓣蛋白质含量,研究不同浓度配比的蔗糖、KH2PO4、8–羟基喹啉（8-HQ）和柠檬酸（CA）保鲜液对保鲜效果的影响。结果表明：2%蔗糖 + 150 mg · L-1 KH2PO4 + 120 mg · L-1 8-HQ + 30 mg · L-1 CA保鲜液处理比蒸馏水对照明显延长瓶插寿命6 d,使切花鲜样质量、蛋白质含量和相对电导率变化比较慢,适于龙船花瓶插保鲜。 相似文献
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采用4种不同瓶插保鲜剂配方对香石竹切花保鲜效果进行了比较研究。结果表明:4种瓶插保鲜剂均明显改善了香石竹切花体内水分状况,花朵开放度增大,切花瓶插寿命延长,POD酶活性提高,细胞膜透性增加延缓。处理A(3%蔗糖+200 mg/L 8-HQ+40 mg/L苯甲酸+300 mg/L青霉素)、处理B(3%蔗糖+200 mg/L 8-HQ+40 mg/L苯甲酸+100 mg/L赤霉素)、处理C(3%蔗糖+200 mg/L 8-HQ+40 mg/L苯甲酸+50 mg/L 6-BA)效果更加明显。处理A、处理B和处理C在延长切花瓶插寿命、提高POD酶活性上无明显差异,但处理A和处理C在增大花径、改善水分平衡和降低细胞膜透性方面要优于处理B。在延缓膜透性增加上,处理C的效果更佳。 相似文献
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壳聚糖对“西伯利亚”百合切花保鲜效应研究 总被引：3，自引：0，他引：3
以自来水、蔗糖、8-羟基喹啉(8-HQ)和柠檬酸不添加量为保鲜液的基本配方,分别加入不同浓度的羧甲基壳聚糖(CMCS)制成鲜切花保鲜剂,对“西伯利亚”百合切花进行瓶插试验.通过观察测量瓶插百合鲜切花外观品质、丙二醛(MDA)含量、可溶性蛋白质含量和膜透性等形态指标和生理指标,以期明确壳聚糖对百合鲜切花的保鲜效应.结果表明:添加了不同浓度.CMCS的各处理都能明显延长百合鲜切花的瓶插寿命,尤其以添加0.10 g/L CMCS的处理3保鲜效果最好,能明显延迟花朵开放、保持切花水分平衡、抑制呼吸高峰、平衡细胞膜相对透性,使百合鲜切花瓶插寿命长达15 d,比基本配方瓶插寿命延长7d,比自来水对照延长8d. 相似文献
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蒟蒻薯（Tacca chantrieri Andre）是观赏价值较高的稀有黑色切花,但瓶插寿命较短,对其进行保鲜研究具有重要意义。用蔗糖、8–羟基奎宁（8-HQ）和柠檬酸按三因素二水平的正交设计配制瓶插保鲜液,通过观察切花形态变化,测定瓶插寿命和鲜样质量,研究瓶插液的保鲜效果。结果表明,与对照相比,各处理均能不同程度地延缓切花衰老,其中A处理,即2%蔗糖 + 100 mg · L-1 8-HQ + 200 mg · L-1柠檬酸 + 50 mg · L-1 6-BA + 100 mg · L-1 K2HPO4处理效果最好,花枝寿命和花须寿命分别比对照长17 d和14 d,是蒟蒻薯切花较好的瓶插保鲜配方。 相似文献
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几种保鲜液对粉花绣线菊切花保鲜效果的研究 总被引：1，自引：1，他引：0
以蔗糖(S)、8-羟基喹啉(8-HQC)、硫代硫酸银(STS)、6-苄基腺嘌呤(6-BA)的4种溶液,根据不同的浓度和组合配制成13种保鲜液,并用蒸馏水作为对照对粉花绣线菊进行瓶插试验,研究切花绣线菊的瓶插寿命。结果表明:以8 g/L S+200 mg/L 8-HQC为配方的处理对切花绣线菊的保鲜效果最好,它能显著地延长该切花的瓶插寿命,增大开花率,增加切花的观赏价值。STS不能作为粉花绣线菊的保鲜剂,它促进切花衰亡。 相似文献
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AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
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多效唑对猕猴桃离体试管苗生长及内源激素的影响 总被引：18，自引：0，他引：18
多效唑（ＰＰ３３３）处理猕猴桃试管苗，降低了其生长强度；植株体内的ＧＡ３、ＩＡＡ和ＺＴ含量下降，ＡＢＡ的含量上升，乙烯释放率增加；并且能降低外源的ＧＡ３和ＩＡＡ促进生长的作用，而外源的ＧＡ３和ＩＡＡ又能不同程度地逆转多效唑的抑制作用，使植株恢复生长。 相似文献
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HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献
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Duncan Brean W. Boyle Shannon Breininger David R. Schmalzer Paul A. 《Landscape Ecology》1999,14(3):291-309
Historic landcover dynamics in a scrubby flatwoods (Tel-4) and scrub landscape (Happy Creek) on John F. Kennedy Space Center were measured using aerial images from 1943, 1951, 1958, 1969, 1979, and 1989. Landcover categories were mapped, digitized, geometrically registered, and overlaid in ARC/INFO. Both study sites have been influenced by various land use histories, including periods of range management, fire suppression, and fire management. Several analyses were performed to help understand the effects of past land management on the amount and spatial distribution of landcover within the study sites. A chi-squared analysis showed a significant difference between the frequency of landcover occurrence and management period. Markov chain models were used to project observed changes over a 100-year period; these showed current management practices being effective at Tel-4 (restoring historic landscape structure) and much less effective at Happy Creek. Documenting impacts of past management regimes on landcover has provided important insight into current landscape composition and will provide the basis for improving land management on Kennedy Space Center and elsewhere. 相似文献
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AIM: Previous studies performed with XBP-01 in vitro indicated that XBP-01 could inhibit vascular smooth muscle cells from being transformed into foam cell and could eliminate the atherosclerotic plaque in C57BL/6J mouse. This experiment is to investigate its mechanism of eliminating plaques in vitro. METHODS: The cultured porcine artery smooth muscle cells incubated with XBP-01 of 0.1 mg/L for 24 h after preincubated with oxidized low density lipoprotein of 15 mg/L for 72 h in vitro. The samples were analyzed by fluorescence microscope, confocal microscope system and flow cytometry. RESULTS: Apoptosis was triggered by being incubated with oxidized low density lipoprotein and this process was accelerated additionally by being incubated with XBP-01. CONCLUSION: XBP-01 can be effective in eliminating atherosclerotic plaque by accelerating the process in which oxidized low density lipoprotein induced smooth muscle cell apoptosis. 相似文献
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Species distribution models (SDMs) often use elevation as a surrogate for temperature or utilise elevation sensitive interpolations
from weather stations. These methods may be unsuitable at the landscape scale, especially where there are sparse weather stations,
dramatic variations in exposure or low elevational ranges. The goal of this study was to determine whether radiation, moisture
or a novel estimate of exposure could improve temperature estimates and SDMs for vegetation on the Illawarra Escarpment, near
Sydney, Australia. Forty temperature sensors were placed on the soil surface of an approximately 12,000 ha study site between
November 2004 and August 2006. Linear regression was used to determine the relationship with environmental factors. Elevation
was correlated more with moderate temperatures (winter maximums, summer minimums, spring and autumn averages) than extreme
temperatures (summer maximums, winter minimums). The correlation (r
2) between temperature and environmental factors was improved by up to 0.38 by incorporating exposure, moisture and radiation
in the regressions. Summer maximums and winter minimums were predominately determined by exposure to the NW and coastal influences
respectively, while exposure to the NE and SW was important during other seasons. These directions correspond with the winds
that are most influential in the study area. The improved temperature estimates were used in Generalised Additive Models for
37 plant species. The deviance explained by most models was increased relative to elevation, especially for moist rainforest
species. It was concluded that improving the accuracy of seasonal temperature estimates could improve our ability to explain
the patchy distribution of many species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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AIM: To detect the expression of cytoplasmic inhibitor of apoptosis protein 2 (c-IAP2) and growth arrest-specific gene 1 (GAS1) in Hodgkin's lymphoma (HL) and anaplastic large cell lymphoma (ALCL) and to investigate the role of two genes in the pathogenesis of HL and ALCL.METHODS: HE staining, the antibodies CD30, CD15, CD45RO and CD20 were used to screen the cases of HL and ALCL from 288 cases of lymphoma. The clarified HL and ALCL were subjected for immunohistochemical staining by SP and ABC methods to analyze the expression of c-IAP2 and GAS1. RESULTS: ①The positive rate of c-IAP2 in HL was 25/26(96.1%) while that in ALCL was 6/19(31.6%), there presented statistic significance between HL and ALCL groups(P<0.05), meanwhile the positive rate of GAS1 showed statistic significance between HL and ALCL groups(P<0.05). ②Two cases were showed to be a mixed type combined with large tumor cells of HL and relatively smaller tumor cells of ALCL.CONCLUSION: ①The different expression of c-IAP2 and GAS1 in HL and ALCL implied a different mechanism of oncogenesis and the different defects in the pathway of signal transduction of apoptosis in HL and ALCL;②Few cases showed an overlap and a likely transitional state between HL and ALCL; ③The different expressing manner of GAS1 and c-IAP2 in HL and ALCL implied the potential marks for the differential dignosis of two kinds of lymphoma. 相似文献
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Effects of patch attributes, barriers, and distance between patches on the distribution of a rock-dwelling rodent (Lagidium viscacia) 总被引：1，自引：0，他引：1
We tested whether size of habitat patches and distance between patches are sufficient to predict the distribution of the mountain
vizcacha Lagidium viscacia a large, rock-dwelling rodent of the Patagonian steppe Argentina, or whether information on other patch and landscape characteristics
also is required. A logistic regression model including the distance between rock crevices and depth of crevices, distance
between a patch and the nearest occupied patch, and whether or not there was a river separating it from the nearest occupied
patch was a better predictor of patch occupancy by mountain vizcachas than was a model based only on patch size and distance
between patches. Our results indicate that a simple metapopulation analysis based on size of habitat patches and distance
between patches may not provide an accurate representation of regional population dynamics if patches vary in habitat quality
independently of patch size and features in the matrix alter connectivity.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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β3-adrenergic receptor is the third subtype of β-adrenergic receptors. The genetic structure and pharmacological property of β3-adrenergic receptor are markedly distinguished from β1-and β2-adrenergic receptor subtypes. Recently studies show that myocardial β3-adrenergic receptor mediates negative inotropic effect through Gi-protein/NO/cGMP pathway, the expression of β3-adrenergic receptor and negative inotropic effect mediated by β3-adrenergic receptor are increased in heart failure. However, because of the low expression of β3-adrenergic receptor in the heart, the actual pathophysiological significance of β3-adrenergic receptor remains unknown. 相似文献